• 한국어류학회지
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• 제26권4호
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• pp.249-258
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• 2014

금강에서 채집된 Acheilognathus sp.의 분류학적 위치를 밝히기 위하여 이들의 형태적 및 유전학적 특성을 검토하고 속 내의 유사종인 줄납자루 및 큰줄납자루와 비교하였다. 이들은 많은 형태적인 특징에서 거의 비슷하였으나 수컷의 혼인색인 등지느러미와 뒷지느러미 가장자리에 붉은 띠가 있고, 배지느러미 가장자리에 흰 띠가 있으며, 최대 체장이 줄납자루보다 큰 점, 계수형질에서는 척추골수가 다소 많은 점과 새파수는 적은 점 등의 특징에서 A. sp.가 다른 두 종과 차이가 있었다. 계통 분석에 의하면 A. sp.는 큰줄납자루 및 A. cyanostigma와 단계통을 형성하였으며 줄납자루와는 Acheilognathus속 내의 다른 종들보다도 더 먼 관계를 가지고 있었다. 따라서 금강의 A. sp.는 줄납자루, 큰줄납자루 및 일본의 A. cyanostigma와 형태, 분포 및 유전적 특성에 의하여 구별되는 다른 종의 수준에 있다고 생각된다.

• 자원환경지질
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• 제8권4호
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• pp.183-201
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• 1975

The south and southwestern parts of Jeonra-namdo has been known as an alunite province in Korea. The alunite deposits investigated for the present study are Okmaisan, Seongsam, Bugog, Gasado south, Gasado north, Jangsando, Dogcheon and Jungyongri deposits. The main purpose of this study is to depict the genetical origin of the alunite deposits. The rocks distributed in the areas mentioned above consist chiefly of rhyolitic tuff, breccia tuff and andesitic tuff of Cretaceous age which represent different episodes of volcanic activities during Cretaceous epoch. The attitude of bedding of the tuffaceous rocks varies from place to place but generally dips very gently. The alunite deposits are embedded mostly in the rhyolitic tuff so that they appear as layered deposits, this occurrence may be the result of stratigraphic and lithologic controls. The result of this study can be summarized as below. The mineral sequence studied by the mineral paragenesis and the result of the spectrograph anlyses is such that (1) alunite was formed at first and pyrophyllite was nearly contemporaneous with alunite but pyrophyllite formation can be recognized as a secondary mineralization products, (2) kaoline was succeeded to form later and hematite finally deposited, and (3) pyrite was deposited from the begining to the end of the above mineralization period. The compositional change of host rocks is such that CaO, $SiO_2$ and $Na_2O$ were largely removed from the parent rocks and some $Al_2O_3$ and $SO_3$ were transported by the solution so as to enrich the rocks. The sequencial process of such mineralization has resulted in forming those distinguish mineral zones; alunite, kaoline, pyrophyllite, silicifide and sulphide zone which manifest irregular shape. These deposits were formed by hydrothermal solution which was possibly low temperature and contained sulphuric acid originated from $H_2S$ and $SO_2$ gases.

• Journal of Animal Science and Technology
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• 제46권5호
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• pp.841-848
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• 2004

살균된 우유에 오염된 Salmonella의 신속한 검출 방법을 공중 보건의 보호를 위하여 중요하다. RT-PCR 방법은 생균과 사균을 분별하여 검출할 수 있는 분자유전학적 방법이다. 본 연구의 RT-PCR 방법은 Salmonella의 type 1 fImbriae의 단량체 단백질을 암호화하는 fimA 유전자의 mRNA를 주형으로 하여 DNA를 증폭하는 방법으로서 활력이 있는 Salmonella를 검출할수 있기 위하여 개발되었다. RNA 시료를 RQRNase-free DNase로 처리하여 오염된 DNA를 파괴하여 RT-PCR 반응에서 주형으로서 DNA가 합성되는 것을 방지할 수 있었다. 이 RT-PCR 방법은 Salmonella 7균주를 검출하였으나 Escherichia coli, Shigella sonnei, Citrobacter freundii, 및 Klebsiella pneumoniae 는 검출하지 않았다. 우유 내에서 열처리된 $10^7/ml과 10^6/ml$ 세균수의 Salmonella는 검출되 었으나 $10^5{\sim}100/ml$는 검출되지 않았다. RT-PCR를 검출할 수 있는 활력이 있는 Salmonella의 최소 세균수는 100/ml이었다.

• 미생물학회지
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• 제38권3호
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• pp.173-179
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• 2002

발광 박테리아인 Photobacterium species의 lux 오페론에서 발견된 riboflavin 생합성에 관여하는 유전자들(ribI,II,III,IV)의 기능을 조사하였다. 대장균에서 이들 유전자가 포함된 재조합 플라스미드를 발현시켰을 때 상당량의riboflavin이 합성되는 것을 확인하였으며, 또한 이들 유전자들(ribI,II,III,IV)의 기능을 riboflavin에 대하여 종속 영양체인 대장균 돌연변이주(BSV 11,18)를 이용한 유전학적인 방법과 생화학적 방법으로 분석한 결과, 이들은 각각 riboflavin synthase, 3,4-dihydroxy-2-butanone 4-phosphate (DHBP) synthase, lumazine synthase, GTP cyclohydrolase II활성도를 갖는 단백질을 코드하는 것으로 밝혀졌다. 이는Photobacterium species의 riboflavin 유전자 체계가 riboflavin 생합성에 관여하는 모든 5개의 유전자들이 한 오페론에 존재하는 Bacillus subtilis와 주요 riboflavin 유전자들이 분리되어 있는 대장균과는 다른, 중간적인 형태를 갖는다는 것을 나타낸다.

• 한국잔디학회:학술대회논문집
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• 한국잔디학회 2002년도 제15차 한국잔디학회 정기총회 및 춘계학술발표회
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• pp.3-5
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• 2002

This study was carried out to get better understandings about morphological, ecological, and genetical characteristics of annual bluegrass collected from different golf courses in Korea and eventually to establish a successful control strategy. Twenty five local lines of annual bluegrass collected from 20 golf courses in Korea were classified into annual or perennial type on the basis of morphological characteristics. Twelve local lines showing obvious morphological differences were selected and then genetically assessed using RAPD analysis. Classification of the 12 local lines through RAPD analysis were considerably similar to that determined by both of morphological differences and phenotype. Responses of the two types of annual blugrass to herbicides were also examined. Shoot growth of annual bluegrass was significantly suppressed by flazasulfuron and the annual type was more susceptible than perennial type, regardless of flazasulfuron concentrations used. By pendimethalin treatment, there was no clear difference in susceptibility between the two types of annual bluegrass. However, by the treatment of dithiopyr, annual type was more sensitive than perennial type in both shoot and root growth. Nine tree species were screened to detect their allelopathic potential on turfgrasses and annual bluegrass. Acacia (Robinia pseudo-acacia) leaves showed selective inhibition in the shoot and root growth as well as their seed germination when treated with 2% and 10%(v/v) of the extract. However, the other leaf extracts except acacia inhibited non-selectively the growth of three turfgrass species such as bentgrass, perennial ryegrass and zoysiagrass and annual bluegrass. The PAL activities of annual bluegrass increased at 24 h after treatment of acacia leaf extract and peaked at 36 h and then decreased till 60h. The highest PAL activity was observed at 36h after treatment of 10%. The highest activity of CA4H in annual bluegrass was observed at 2h after treatment of acacia extract and the level was 4 times greater than that of the control. The phenolic acids such as p-coumaric acid, salicylic acid and ferulic acid were increased with the treatment of acacia leaf extract. The chloroplast membrane and cell wall of annual bluegrass were destroyed by treatment of acacia leaf extract and its inner materials were released. The membranes in annual bluegrass cells might be destroyed by phytotoxic compounds from acacia leaf extract.

• 암석학회지
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• 제1권1호
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• pp.58-70
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• 1992

한국 남서부의 점토광상들과 주변암에 대한 K-Ar 연대측정 결과, 점토광물이 75.1∼81.4 Ma, 백악기 화산암류(황산층)가 81.4∼86.4 Ma, 백악기 화강암류는 77.1∼81.5 Ma의 연대로 나타났다. 이 결과는 점토광상들이 백악기 후기의 산성마그마티즘과 성인적으로 밀접한 관계가 있음을 지시한다. 월각산 화강암은 K-Ar 흑운모의 연령이 140.g∼144.8 Ma로 밝혀져, 이의 관입연대는 쥬라기로 판명되었다. 이곳의 점토광상들과 백악기 화강암류의 밀접한 관계는 점토광상들이 화산활동에 수반된 열수작용에 의한 것이 아니라 화강암류의 관입에 따른 열적 효과(열수 순환)에 기인된 열수변질작용으로 형성되었다.

• 한국물환경학회지
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• 제34권6호
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• pp.661-668
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• 2018

Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was $17,535ngL^{-1}$ and $14,311ngL^{-1}$ respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin.

• Fisheries and Aquatic Sciences
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• 제21권12호
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• pp.37.1-37.10
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• 2018

The larvae and juveniles of Porocottus leptosomus belonging to the family Cottidae were collected (n = 95, 3.9-16.5 mm in body length, BL) from Busan, Korea, in March 2015. The larvae and juvenile were identified using DNA barcoding as P. leptosomus, and their morphological description was presented in detail. The yolk-sac larvae (3.9-5.6 mm BL) body was slightly compressed, the head was large, the eye was round and large, and the anus was before the middle of the body. The preflexion larvae (5.2-10.0 mm BL) body length drastically increased; caudal fin rays began to occur. The flexion larvae (9.4-11.8 mm BL) notochord flexion started; dorsal, pectoral, and anal fin rays began to occur; pelvic fin buds are seen; they possessed a pair of parietal spine; and a pair of supraocular cirri was first to develop. At 12 mm BL, the notochord was completely flexed. The larva stage (3.9-12.6 mm SL) had the stellate melanophores in the head, isthmus, gut, and tail (along to the ventral midline). During the juvenile stage (11.4-16.5 mm BL), melanophores covered the head and began to form five black bands on the side of the body. The larvae of P. leptosomus spent pelagic life, but moved to the bottom during the juvenile stage. The larvae and juveniles of P. leptosomus differ from other cottid larval fishes by body shape, melanophore head pattern, and spine development. P. leptosomus can be distinguished from Porocottus allisi by morphological development and the occurrence of larval fish: preopercular spine development, melanophore pattern, and caudal fin development.

• Biomolecules & Therapeutics
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• 제31권5호
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• pp.484-495
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• 2023

Idiopathic pulmonary fibrosis (IPF) can be defined as a progressive chronic pulmonary disease showing scarring in the lung parenchyma, thereby resulting in increase in mortality and decrease in the quality of life. The pathophysiologic mechanism of fibrosis in IPF is still unclear. Repetitive microinjuries to alveolar epithelium with genetical predisposition and an abnormal restorative reaction accompanied by excessive deposition of collagens are involved in the pathogenesis. Although the two FDA-approved drugs, pirfenidone and nintedanib, are under use for retarding the decline in lung function of patients suffered from IPF, they are not able to improve the survival rate or quality of life. Therefore, a novel therapeutic agent acting on the major steps of the pathogenesis of disease and/or, at least, managing the clinical symptoms of IPF should be developed for the effective regulation of this incurable disease. In the present review, we tried to find a potential of managing the clinical symptoms of IPF by natural products derived from medicinal plants used for controlling the pulmonary inflammatory diseases in traditional Asian medicine. A multitude of natural products have been reported to exert an antifibrotic effect in vitro and in vivo through acting on the epithelial-mesenchymal transition pathway, transforming growth factor (TGF)- β-induced intracellular signaling, and the deposition of extracellular matrix. However, clinical antifibrotic efficacy of these natural products on IPF have not been elucidated yet. Thus, those effects should be proven by further examinations including the randomized clinical trials, in order to develop the ideal and optimal candidate for the therapeutics of IPF.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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• pp.37-43
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• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7~8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr. Hashiyada(2001), 296 pairs of split-half embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs. Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1988, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a glaf of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us as effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle. 6. Farm animal cloning is one of the most dreamful technologies of 21th century. It is necessary to develop this technology more efficient and stable as realistic technology of the farm animal production. We are making researches related to the best condition of donor cells for high productivity of cloning, genetic analysis of cloned animals, growth and performance abilities of clone cattle and pathological and genetical analysis of high rates of abortion and stillbirth of clone calves (about 30% of periparutum mortality). 7. It is requested in the report of Ministry of Health, labor and Welfare to make clear that carbon-copy cattle(somatic cell clone cattle) are safe and heathy for a commercial market since the somatic cell cloning is a completely new technology. Fattened beef steers (well-proved normal growth) and milking cows(shown a good fertility) are now provided for the assessment of food safety.