Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetical identification and morphological description of the larvae and juveniles of Porocottus leptosomus (Pisces: Cottidae) from Korea

  • Shin, Ui Cheol (Dokdo Fisheries Research Center, National Institute of Fisheries Science) ;
  • Jeong, Yeon Kyu (Dokdo Fisheries Research Center, National Institute of Fisheries Science) ;
  • Yoon, Sang Chul (Marine and Fisheries Bio-resources Division, Minister of Oceans and Fisheries) ;
  • Choi, Kwang Ho (Dokdo Fisheries Research Center, National Institute of Fisheries Science) ;
  • Kim, Jin-Koo (Department of Marine Biology, Pukyong National University)
  • Received : 2018.10.14
  • Accepted : 2018.12.07
  • Published : 2018.12.31
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Abstract

The larvae and juveniles of Porocottus leptosomus belonging to the family Cottidae were collected (n = 95, 3.9-16.5 mm in body length, BL) from Busan, Korea, in March 2015. The larvae and juvenile were identified using DNA barcoding as P. leptosomus, and their morphological description was presented in detail. The yolk-sac larvae (3.9-5.6 mm BL) body was slightly compressed, the head was large, the eye was round and large, and the anus was before the middle of the body. The preflexion larvae (5.2-10.0 mm BL) body length drastically increased; caudal fin rays began to occur. The flexion larvae (9.4-11.8 mm BL) notochord flexion started; dorsal, pectoral, and anal fin rays began to occur; pelvic fin buds are seen; they possessed a pair of parietal spine; and a pair of supraocular cirri was first to develop. At 12 mm BL, the notochord was completely flexed. The larva stage (3.9-12.6 mm SL) had the stellate melanophores in the head, isthmus, gut, and tail (along to the ventral midline). During the juvenile stage (11.4-16.5 mm BL), melanophores covered the head and began to form five black bands on the side of the body. The larvae of P. leptosomus spent pelagic life, but moved to the bottom during the juvenile stage. The larvae and juveniles of P. leptosomus differ from other cottid larval fishes by body shape, melanophore head pattern, and spine development. P. leptosomus can be distinguished from Porocottus allisi by morphological development and the occurrence of larval fish: preopercular spine development, melanophore pattern, and caudal fin development.

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References

  1. Blood DM, Matarese AC. Larval development and identification of the genus Triglops (Scorpaeniformes: Cottidae). NOAA Prof. Paper, NMFS. 2010;10:1-43.
  2. Cartwright RL. Description of early life history stages of the northern sculpin (Icelinus borealis Gilbert) (Teleostei: Cottidae). Fish Bull. 2009;107:175-86.
  3. Choi Y, Lee HH, Jang JH. Ichthyofauan of the intertidal zone around the nuclear power plants off Sinweolseong, Eastern Coast, Korea. Korean J. Ichthyol. 2008;20:313-7.
  4. Choi Y, Yang ZF. Intertidal fishes from the Shandong Peninsula, China. Korean J Ichthyol. 2008;20:54-60.
  5. Eschmeyer WN. Western Atlantic scorpionfishes of the genus Scorpaena, including four new species. Bull Mar Sci. 1965;15:84-164.
  6. Eschmeyer WN. A field guide to Pacific coast fishes: North America. Houghton Mifflin, Boston: Mass; 1999.
  7. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp. 1999;41:95-8.
  8. Han KH, Kim YU. Development of larvae and Juvenils of the Cottid fish, Porocottus tentaculatus (Kner). Bull. Yosu. Nat'l fish. Univ. 1997;11(2):119-29.
  9. Ivanova NV, Zemlak TS, Hanner RH, Hebert PDN. Universal primer cocktails for fish DNA barcoding. Mol Ecol Notes. 2007;7:544-8. https://doi.org/10.1111/j.1471-8286.2007.01748.x
  10. Kendall AW Jr, Lenarz WH. Status of early life history studies of northeast Pacific rockfishes. In: Proceedings of the International Rockfish Symposium. Anchorage, Alaska: University of Alaska, Alaska Sea Grant College Program Report AK-SG-87-2; 1986. p. 99-128.
  11. Kendall AW Jr. Identification of eggs and larvae of marine fishes: Tokai Univ Press; 2011.
  12. Kim JK, Ryu JH, Kim S, Lee DW, Choi KH, Oh TY, Hwang KS, Choi JH, Kim JN, Kwun HJ, Ji HS, Oh JN. An identification guide for fish eggs, larvae and juveniles of Korea. Busan: Hanguel Graphics Publishing Co Ltd; 2011.
  13. Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111-20. https://doi.org/10.1007/BF01731581
  14. Knope ML. Phylogenetics of the marine sculpins (Teleostei: Cottidae) of the North American Pacific coast. Mol Phylogenet Evol. 2013;66:341-9. https://doi.org/10.1016/j.ympev.2012.10.008
  15. Kurawaka K. 1976. Study of speciation in fish. Unpubl. Ph. D. Dissertation, Kyoto Univ., 55: pls. 1-31.
  16. Kyshin K. Embryonic development and larvae of Gymnocanthus herzensteini Jordan and Starks. Japan J Ichthyol. 1970;17:74-9.
  17. Marliave JB. Lack of planktonic dispersal of rocky intertidal fish larvae. Trans Am Fish Soc. 1986;115:149-54. https://doi.org/10.1577/1548-8659(1986)115<149:LOPDOR>2.0.CO;2
  18. Matarese AC, Kendall AW Jr, Blood DM, Vinter BM. Laboratory guide to early life history stages of Northeast Pacific fishes. NOAA Tech Rep, NMFS. 1989;80:1-652.
  19. Moser HG, Ahlstrom EH. Larvae and pelagic juveniles of blackgill rockfish, Sebastes melanostomus, taken in midwater trawls off southern California and Baja California. J Fish Res Bd Can. 1978;35:981-96. https://doi.org/10.1139/f78-160
  20. Muto F, Choi Y, Yabe M. Porocottus leptosomus sp. Now., from the west coast of Korea, Yellow Sea (Scorpaeniformes: Cottidae). Ichthyol Res. 2002;49:229-33. https://doi.org/10.1007/s102280200032
  21. Muto F, Yabe M, Amaoka K. A new cottid fish, Artediellus neyelovi, from the southeastern coast of the Oshima Peninsula, Hokkaido, Japan., Jpn. J Ichthyol. 1994;41:275-80.
  22. Nakabo T, Kai Y. Cottidae. In: Nakabo T, editor. Fishes of Japan with pictorial keys to the species. third ed. ToKyo, JP: Tokai Univ Press; 2013. p. 628-50.
  23. Nelson JS. Fishes of the world. fourth ed. New York: Wiley; 2016.
  24. NIBR (National Institute of Biological Resources). National lists of species of Korea. Incheon: Vertebrate Natl. Inst. Biol. Res; 2011. p. 81-6.
  25. Okiyama M. An atlas of the early stage fishes in Japan. Tokyo: Tokai University Press; 2014.
  26. Ramon ML. Molecular ecology of intertidal sculpins, PhD thesis, UCSC; 2007.
  27. Richardson SL. Current knowledge of larvae of sculpins (Pisces: Cottidae and allies) in Northeast Pacific genera with notes on intergeneric relationships. Fish Bull. 1981;79(1):103-21.
  28. Sado T, Tachibana Y, Kimura S. Developmental morphology of the cottid fish Pseudoblennius marmoratus. Ichth Res. 2005;52:292-6. https://doi.org/10.1007/s10228-005-0275-5
  29. Shin UC, Park JH, Kim JK. New Korean record of Artediellus fuscimentus (Scorpaeniformes: Cottidae). Korean J. Ichthyol. 2016;28(3):207-12.
  30. Song YS, Kim JK, Ryu JH, Kim HJ, Kweon SM, Choi SH. First record of Icelus toyamensis (Scorpaeniformes: Cottidae) from the East Sea, Korea. Korean J Syst Zool. 2012;28(4):304-7.
  31. Stein R. Description of lavoratory-reared larvae of Oligocottus maculosus Girard (Pisces: Cottidae). Copeia. 1973;1973(2):373-7. https://doi.org/10.2307/1442990
  32. Takeshita N, Onikura N, Matsui S, Kimura S. Embryonic, larval and juvenile development of the roughskin sculpin, Trachidermus fasciatus (Scorpaeniformes: Cottidae). Japan. J. Ichthyol. 1997;44(3):257-66.
  33. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  34. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673-80. https://doi.org/10.1093/nar/22.22.4673
  35. Waples RS. A multispecies approach to the analysis of gene flow in marine shore fishes. Evolution. 1987;41:385-400. https://doi.org/10.1111/j.1558-5646.1987.tb05805.x
  36. Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD. DNA barcoding Australia's fish species. Philos Trans R Soc B. 2005;360:1847-57. https://doi.org/10.1098/rstb.2005.1716
  37. Watanabe M. Fauna Japonica: Cottidae (pisces). Tokyo: Tokyo News Service; 1969.
  38. Yabe M. Comparative osteology and myology of the superfamily Cottoidea (Pisces: Scorpaeniformes), and its phylogenetic classification. Faculty of Fisheries. Hakodate: Hokkaido University; 1985.
  39. Yoshiyama RM, Gaylord KB, Philippar MT, Moore TR, Jordan JR, Coon CC, Schalk LL, Valpey CJ, Tosques I. Homing behavior and site fidelity intertidal sculpins (Pisces: Cottidae). J Exp Mar Biol Ecol. 1992;160:115-30. https://doi.org/10.1016/0022-0981(92)90114-P