• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.611-616
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• 2006

Genetic parameters for performance traits on 409 bulls and growth and carcass traits on 591 of their steer progeny were estimated in Japanese Black cattle with Gibbs sampling. Traits of bulls included body weight at the start (BWS) and finish (BWF) of test, daily gain (DG), concentrate, roughage and TDN intake, and TDN conversion ratio. Progeny traits were BWS, BWF, DG, rib eye area, marbling score (MSR), dressing percentage and subcutaneous fat thickness (SFT). In bulls, heritabilities were high for BWS (0.50) and BWF (0.63) and moderate for concentrate (0.48) and TDN intake (0.45), while in progeny, the heritability for all the studied traits was moderate to high (ranging from 0.30 to 0.73), highlighting the potential for genetic improvement of these traits. Genetic correlations between TDN intake and growth traits (BWS, BWF and DG) in bulls were highly positive (ranging from 0.77 to 0.94). The weak but negative genetic correlation (-0.20) between MSR and SFT in progeny indicated that improvement of beef marbling without increasing subcutaneous fat deposition could be possible. The estimated genetic correlations of roughage intake of bulls with body weights (BWS and BWF) and MSR of their progeny were moderate (ranging from 0.35 to 0.52). On the basis of the selection for bulls, growth traits and TDN intake correlated positively with SFT (ranging from 0.43 to 0.53) of their progeny, suggesting the necessity of controlling the increase of SFT in selection programs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.446-451
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• 2019

This study investigated the application of artificial insemination and pregnancy diagnosis kit for Korean native black goats. Semen was collected by electrical ejaculation, followed by semen analysis and artificial insemination in three goat strains (Dangjin, Jangsu and Tongyoung). Pregnancy was confirmed using a cow pregnancy test kit (IDEXX Rapid Visual Pregnancy Test kit) and ultrasound diagnosis. Analysis revealed that semen collected from male Korean native black goats by electrical ejaculation was about 1~1.5 ml in volume, $18{\sim}25{\times}10^8/ml$ concentration, and having > 97% motility. Furthermore, confirmation of pregnancy by pregnancy test kit and ultrasound diagnosis after artificial insemination were similar. In addition, the efficiency of pregnancy was 20~40% for all three strains: Tongyoung was the highest with 44%, followed by Dangjin (%), and Jangsu (20%). This study determines the artificial fertilization efficiency and the feasibility of using a cow pregnancy test kit for early pregnancy diagnosis in Korean native black goats. Although further research is required for validation, the results of the current study contribute to the breeding and improvement of Korean native black goat in research institutions as well as in general farms.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.3
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• pp.154-161
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• 2005

The durability of a vehicle is a very important performance which can be evaluated from endurance test. This study developed a procedure for determination of a duty cycle theoretically. Vehicle load data is classified and rearranged using standard test road profile. A load pattern and a duty cycles are extracted from classified vehicle data using genetic algorithm. A duty cycle could be utilized in dynamo test to meet required test mileage. The derived duty cycles have been verified by fatigue test through the dynamometer test.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.6
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• pp.759-767
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• 2016

The aim of this study was to compare two random regression models (RRM) fitted by fourth ($RRM_4$) and fifth-order Legendre polynomials ($RRM_5$) with a lactation model (LM) for evaluating Holstein cattle in Brazil. Two datasets with the same animals were prepared for this study. To apply test-day RRM and LMs, 262,426 test day records and 30,228 lactation records covering 305 days were prepared, respectively. The lowest values of Akaike's information criterion, Bayesian information criterion, and estimates of the maximum of the likelihood function (-2LogL) were for $RRM_4$. Heritability for 305-day milk yield (305MY) was 0.23 ($RRM_4$), 0.24 ($RRM_5$), and 0.21 (LM). Heritability, additive genetic and permanent environmental variances of test days on days in milk was from 0.16 to 0.27, from 3.76 to 6.88 and from 11.12 to 20.21, respectively. Additive genetic correlations between test days ranged from 0.20 to 0.99. Permanent environmental correlations between test days were between 0.07 and 0.99. Standard deviations of average estimated breeding values (EBVs) for 305MY from $RRM_4$ and $RRM_5$ were from 11% to 30% higher for bulls and around 28% higher for cows than that in LM. Rank correlations between RRM EBVs and LM EBVs were between 0.86 to 0.96 for bulls and 0.80 to 0.87 for cows. Average percentage of gain in reliability of EBVs for 305-day yield increased from 4% to 17% for bulls and from 23% to 24% for cows when reliability of EBVs from RRM models was compared to those from LM model. Random regression model fitted by fourth order Legendre polynomials is recommended for genetic evaluations of Brazilian Holstein cattle because of the higher reliability in the estimation of breeding values.

• Journal of Korean Society of Forest Science
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• v.103 no.1
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• pp.51-58
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• 2014

Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendron amurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon's diversity index(I) was 0.421 and the expected heterozygosity($H_e$) was 0.285, which was similar to the heterozygosity (hs =0.287) inferred by Bayesian method. In AMOVA, 7.6% of total genetic variation in the populations was resulted from the genetic difference among populations and the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(${\theta}^{II}$) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first group included Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of the geographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel's test. For conservation of the phellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.

• Toxicological Research
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• v.34 no.3
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• pp.259-266
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• 2018

Glycomacropeptide (GMP), a whey protein of milk, has functions including differentiation and development of nervous system, and anticancer and antiviral effects. To develop new functions, N-acetylneuraminic acid (NANA) containing 7% sialic acid was separated from GMP to produce G-7%NANA. N-glycolylneuraminic acid (Neu5Gc) is another type of sialic acid separated from GMP, which has been linked to immune disorders and chronic inflammation-mediated diseases. Therefore, safety was a concern in the use of G-7%NANA in functional foods. To ensure safety, in this study, three genetic toxicity tests on G-7%NANA were conducted. In the reverse mutation test using Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2uvrA, and in the chromosome aberration test using CHO-K1 cells, no significant differences from negative control were found at all dose levels. Similarly, no dose-related differences were evident compared to negative control in the micronucleus test using ICR mice. There was no evidence of G-7%NANA-related genetic toxicity.

• Biomolecules & Therapeutics
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• v.15 no.3
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• pp.192-198
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• 2007

Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a major constituent of rhubarb. Although it has been claimed to have a wild spectrum of therapeutic value, its side effects, especially in human kidney cells have not been well characterized. In this study, we have carried out in vitro genetic toxicity test of emodin and microarray analysis of differentially expressed genes in response to emodin. The result of Ames test showed mutations with emodin treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, emodin showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with emodin treatment showed DNA damage both with and without exogenous metabolic activation. Emodin did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially expressed genes in response to emodin by microarray analysis and these genes would be candidate biomarkers of genetic toxic action of emodin.

• Journal of the Korea Society of Computer and Information
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• v.10 no.6 s.38
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• pp.85-92
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• 2005

In this Paper, we develope an effective web-based learning contents using interactive genetic algorithms with modified mutation operation. In the interactive genetic algorithm, reciprocal exchange mutation is used. But. we modify the mutation operator to improve the learning effects. The new web-based learning contents using interactive genetic algorithm provide the dynamic learning contents providing and real-time test system. Especially, learners can execute the interactive genetic algorithm according to the learners' characters and interests to select the efficient learning environments and contents sequences.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.198-202
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• 2004

This study proposes a new optimization algorithm which is combined with genetic algorithm and ANOM. This improved genetic algorithm is not only faster than the simple genetic algorithm, but also gives a more accurate solution. The optimizing ability and convergence rate of a new optimization algorithm is identified by using a test function which have several local optimum and an optimum design of rocker arm shaft. The calculation results are compared with the simple genetic algorithm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.1
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• pp.35-42
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• 1998

Eleven Italian ryegrass cultivars were examined for their genetic polymorphisms and phylogenetic relationships using randomly amplified polymorphic DNA (RAPD) markers. In RAPD analysis of 34 random primers, 96 of total 162 bands obtained from 16 primers were polymorphic and sizes of polymorphic band ranged between 0.5 and 1.5kb. Number of bands amplified per primer was varied from 3 to 16 and average number was 14.8. Phylogenetic relationship among cultivars based on the RAPD analysis was examined using UPGMA computer program. In pairwise genetic similarity test of 11 Italian ryegrass cultivars, Grazer and Orlando showed highest coefficient of genetic similarity as 0.740, whereas Marshall and Orlando was lowest as 0.438. Eleven Italian ryegrass cultivars were grouped into 3 major clusters and genetic distance of clusters ranged between 0.567 and 0.646, indicating low level of genetic variation.