Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Genetic Diversity and Genetic Structure of Phellodendron amurense Populations in South Korea

황벽나무 자연집단의 유전다양성 및 유전구조 분석

  • Lee, Jei-Wan (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Hong, Kyung-Nak (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kang, Jin-Taek (Center for Forest & Climate Change, Korea Forest Research Institute)
  • 이제완 (국립산림과학원 산림유전자원과) ;
  • 홍경낙 (국립산림과학원 산림유전자원과) ;
  • 강진택 (국립산림과학원 기후변화센터)
  • Received : 2013.09.11
  • Accepted : 2014.03.14
  • Published : 2014.03.31
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Abstract

Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendron amurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon's diversity index(I) was 0.421 and the expected heterozygosity($H_e$) was 0.285, which was similar to the heterozygosity (hs =0.287) inferred by Bayesian method. In AMOVA, 7.6% of total genetic variation in the populations was resulted from the genetic difference among populations and the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(${\theta}^{II}$) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first group included Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of the geographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel's test. For conservation of the phellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.

본 연구는 ISSR 표지자를 이용하여 국내 분포하는 황벽나무 7개 집단의 유전다양성과 유전구조를 분석하였다. 6개의 ISSR primer를 이용하여 분석한 결과 primer 당 평균 4.5개의 다형성 band를 확인하였고, 각 집단의 다형성 유전자좌의 비율은 평균 78.8%로 나타났다. Shannon의 유전다양성 지수(I)는 0.421로 나타났고, 이형접합체 기대치($H_e$)는 평균 0.285로 베이즈 방법을 이용한 평균 이형접합체 기대치(hs=0.287)와 유사하였다. AMOVA에서 전체 유전변이의 92.4%가 집단내 개체간 차이에 기인하며, 7.6%는 집단간 차이에 기인하였다. 베이즈 방법을 이용한 유전분화(${\theta}^{II}$)는 0.066으로 추정되었으며, 전체 집단의 근친교배율(f)은 0.479로 계산되었다. 유연관계 분석과 베이즈 군집분석결과 우리나라 황벽나무 집단은 가평, 화천, 봉평, 용평이 하나의 군집을 형성하였고, 산청 지역의 2개 집단(삼장 및 시천)이 다른 하나의 군집을 형성하였으며, 무주 집단이 산청지역의 집단과 지리적으로 근접함에도 불구하고 독립적인 군집을 나타내었다. Mantel's test 결과 집단간 유전적 유연관계와 지리적 분포의 상관성은 나타나지 않았다. 황벽나무의 유전자원보존을 위한 대상 집단 선정 시 생태적 및 생활사적 특징과 함께 본 연구결과에서 나타난 유전다양성과 군집구조 분석결과를 고려하는 것이 효과적일 것으로 사료된다.

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