• Animal cells and systems
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• 제16권4호
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• pp.313-320
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• 2012

Genetic variation was surveyed at four microsatellite loci and 1416 base pairs (bp) of the mitochondrial DNA (mtDNA) cytochrome c oxidase I gene (COI) to clarify the genetic structure of the small yellow croaker, Larimichthys polyactis, in the Yellow and East China Seas, especially regarding four provisional populations, (one Korean and three Chinese populations). Based on microsatellite DNA variations, the estimated expected heterozygosity ($H_E$) in each population ranged from 0.776 to 0.947. The microsatellite pairwise $F_{ST}$ estimates showed no significant genetic differentiation between the populations. MtDNA variations also indicated no genetic structure in L. polyactis, but very high variability. The absence of genetic differentiation among and within populations of L. polyactis may either result from the random migration of the adult or the passive dispersal of the eggs and larvae.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권1호
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• pp.32-41
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• 2021

South Korea presently harbors less than 800 long-tailed gorals (Naemorhedus caudatus), an endangered species. I report for the first time on the taxonomic status and genetic diversity of the Korean species using non-invasive fecal sampling based on mitochondrial cytochrome b gene sequence analyses. To determine the taxonomic status of this species, I reconstructed a consensus neighbor-joining tree and generated a minimum spanning network combining haplotype sequences obtained from feces with a new goral-specific primer set developed using known sequences of the Korean goral and related species (e.g., Russian goral, Chinese goral, Himalayan goral, Japanese serow, etc.). I also examined the genetic diversity of this species. The Korean goral showed only three different haplotypes. The phylogenetic tree and parsimony haplotype network revealed a single cluster of Korean and Russian gorals, separate from related species. Generally, the Korean goral has a relatively low genetic diversity compared with that of other ungulate species (e.g., moose and red deer). I preliminarily showcased the application of non-invasive fecal sampling to the study of genetic characteristics, including the taxonomic status and genetic diversity of gorals, based on mitochondrial DNA. More phylogenetic studies are necessary to ensure the conservation of goral populations throughout South Korea.

• ALGAE
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• 제37권2호
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• pp.135-147
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• 2022

The red algal genus Chondrus have long been used as raw materials for carrageenan and dietary fiber in health foods. Despite the importance of genetic information in safeguarding natural seaweed resources, knowledge of the population genetics of Chondrus in the northwest Pacific is limited. In this study, genetic diversity and phylogeographic structure of 45 populations (777 specimens) of Chondrus from Korea, China, and Japan were evaluated based on mitochondrial COI-5P gene sequences, and phylogenetic relationships were confirmed based on plastid rbcL gene sequences. Molecular analyses assigned the specimens in this study to three Chondrus species: C. nipponicus, C. ocellatus, and C. giganteus; phenotype-based species classification was impossible owing to their high morphological plasticity. We found moderate intraspecific genetic diversity and a shallow phylogeographic structure in both for C. nipponicus and C. ocellatus, and low intraspecific genetic diversity in C. giganteus. Each of the three species exhibited high-level intraspecific gene flow among regions based on the most common haplotypes (CN1 for C. nipponicus, CO1 for C. ocellatus, and CG1 for C. giganteus). Our comprehensive genetic information provides insights into the phylogeographic patterns and intraspecific diversity of the economically important Chondrus species. It also highlights the need to conserve existing natural Chondrus resources through continuous monitoring of genetic diversity and phylogeographic pattern.

• 대한수학교육학회지:수학교육학연구
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• 제9권1호
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• pp.133-150
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• 1999

In this study, we tried to make histo-genetic analyses necessary to identify epistemological obstacles on the function concepts. Historical development on the function concept was analysed. From these analyses, we obtain epistemological obstacles as follows: the perception of changes in the surrounding world, mathematical philosophy, number concepts, variable concepts, relationships between independent variables and dependent variables, concepts of definitions.

• Parasites, Hosts and Diseases
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• 제55권4호
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• pp.367-374
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• 2017

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.

• 한국방재학회 논문집
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• 제10권4호
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• pp.9-16
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• 2010

구조물 상시모니터링에서 센서위치 최적화는 모니터링 결과에 중요한 영향을 준다. 따라서 본 논문은 구조계수 또는 손상도를 추정하기 위한 목적으로 동특성 자료를 계측하고자 할 때, 충분한 정보를 획득할 수 있는 최적의 계측점을 선정하는 방법을 제안하였다. 제안된 방법은 유전자 알고리즘을 계측점 선정을 위한 최적화 기법으로 사용하는 것이다. 유전자 알고리즘의 핵심인 적합도함수를 구조계수에 의한 모드벡터의 민감도와 모드벡터의 직교성을 고려할 수 있도록 구성하였다. 간단한 타워 구조물에 대한 예제 해석을 통해 제안된 방법의 타당성을 확인하였다. 적합도함수를 구성하고 있는 모드 민감도와 모드 직교성이 최적 계측점 선정에 어떤 영향을 주는지 예제해석을 통하여 분석하였다. 결론적으로, 제안된 적합도 함수를 사용하면 계측 목적에 타당한 계측점을 선정할 수 있음을 확인하였다.

• Animal Systematics, Evolution and Diversity
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• 제28권3호
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• pp.215-219
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• 2012

To examine genetic divergences of two endemic Sorex caecutiens subspecies from Korea (S. c. hallamontanus in Korean Jeju Island and S. c. annexus in the mainland Korean Peninsula), we obtained partial cytochrome oxidase I (COI) sequences (429 bp) and complete cytochrome b sequences (1,140 bp) from the two Korean subspecies, and we compared these sequences to the corresponding sequences of S. caecutiens, obtained from GenBank. We found that Jeju S. c. hallamontanus is one of three clades within S. caecutiens, with an average Jukes-Cantor distance of 1.57% in the COI sequences and the distance of 2.07% and 11 fixed site differences in the cytochrome b sequences, indicating that Jeju S. c. hallamontanus is one endemic subspecies with concordant genetic distinctness, although further analyses with nuclear DNA sequences are necessary to confirm these findings. However, S. c. annexus from the mainland Korean Peninsula was not divergent from S. c. macropygmaeus from northeastern China and adjacent Russia, indicating that S. c. annexus from the mainland Korean Peninsula is another endemic subspecies with only morphological differences, although it is necessary to reexamine the subspecies status of S. c. annexus.

• 생명과학회지
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• 제14권4호
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• pp.593-607
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• 2004

유해성 적조생물 Cochlodinium polykrikoides/Gyrodinium aurelum을 포함한 43 종류의 와편조류를 대상으로 SSU 부위 유전자를 분석했다. 유전자 염기서열에 의거한 상호 계통수는 parisomny, distance, maxium 방법으로 실행했다. Noctilura scintillans, Gonyaulax spinifera와 Crythecodinium cohnii 종들은 와편모조류 중 가장 유전적으로 먼 것으로 보였다. Alexandrium과 Symbiodinium 종간의 bootstrap는 70% 이상의 상호 단일 계통도를 보인 반면에, Gymnodinium과 Gyrodinium은 근립절약계수와 최대 유사도 방법에서 다형 계통도를 나타내었다. Gyroddinium aurelum과 G. dorsum/G. galathenum의 유전적 분화율은 7.4% (45 bp) 였고, G. aurelum과 G. mikimotoi 상호간에는 0.9% (5bp) 밖에 나타나지 않았다. 또한 C. polykrikoides와 G. aurelum도 5.2% (31bp)로 낮은 유전적 분화율을 보였다. 계통도를 분석한 결과 G. aureolum과 C. polykrikoides는 Gyrodinium 보다 Gymnodinim 속에 훨씬 더 근접하게 나타났다.

• Molecules and Cells
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• 제43권1호
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• pp.86-95
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• 2020

The red-crowned crane (Grus japonensis) is an endangered, large-bodied crane native to East Asia. It is a traditional symbol of longevity and its long lifespan has been confirmed both in captivity and in the wild. Lifespan in birds is known to be positively correlated with body size and negatively correlated with metabolic rate, though the genetic mechanisms for the red-crowned crane's long lifespan have not previously been investigated. Using whole genome sequencing and comparative evolutionary analyses against the grey-crowned crane and other avian genomes, including the long-lived common ostrich, we identified redcrowned crane candidate genes with known associations with longevity. Among these are positively selected genes in metabolism and immunity pathways (NDUFA5, NDUFA8, NUDT12, SOD3, CTH, RPA1, PHAX, HNMT, HS2ST1, PPCDC, PSTK CD8B, GP9, IL-9R, and PTPRC). Our analyses provide genetic evidence for low metabolic rate and longevity, accompanied by possible convergent adaptation signatures among distantly related large and long-lived birds. Finally, we identified low genetic diversity in the red-crowned crane, consistent with its listing as an endangered species, and this genome should provide a useful genetic resource for future conservation studies of this rare and iconic species.

• 한국정보통신학회논문지
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• 제11권5호
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• pp.969-976
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• 2007

유전자 알고리즘을 이용한 다양한 특징의 분석이 필요한 퍼지 분류기의 설계 방법을 제안한다. 본 논문에서 제안한 퍼지 분류기는 퍼지 논리를 이용한 분류 부분과 유전자 알고리즘을 이용한 규칙 생성 부분으로 구성된다. 유전자 알고리즘을 이용한 규칙 생성 부분에서는 최적의 퍼지 멤버쉽 함수를 결정하고, 각 특징이 규칙에 포함되는지 포함되지 않는지의 여부도 결정하게 된다. 또한, 특정 대상에 대한 인식률을 분석하여 큰 오인식률을 갖는 부분에 세부 특징을 추가하는 방법과 문자열과 population의 최소 크기, 인식률 개선을 위한 반복적 분석 방법을 사용한다. 제안된 퍼지 분류기의 적용 예로서, 아이리스 데이터와 갑상선 종양 세포의 식별을 든다. 본 논문에서 제안한 퍼지 분류기는 아이리스 데이터에 대해 98.67%의 인식률을, 갑상선 종양 세포에 대해서 98.25%의 인식률을 얻었다.