• Journal of Korean Medicine Rehabilitation
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• v.34 no.2
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• pp.15-28
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• 2024

Objectives We used the D-galactose (D-gal) induced C2C12 myoblast senescence model to investigate whether ethanol extract of Perilla. fructescens leaves (EEPF) could delay cellular senescence and regulate related mechanisms. Methods C2C12 myogenic cells were cultured in an incubator under 37 ℃ and 5% CO₂ conditions. EEPF, dried perilla leaves were pulverized and extracted at 1:10 (v/v) at 50 ℃ for 4 hours. Cell counting kit-8 and western blot analysis was performed. Annexin V-FITC apoptosis detection kit and DAPI staining was applied. Catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde analysis kits were used. To measure the level of reactive oxygen species generation, staining and flow cytometry was used. To analyze the mitochondrial activity, membrane potential changes were measured using JC-1. 𝛽-gal activity was analyzed using SA-𝛽-gal staining solution, and DNA damage was analyzed by using 𝛾-H2AX. Quantikine ELISA kit was used to analyze inflammatory cytokine production. Results According to the results of this study, EEPF significantly alleviated the decrease in cell viability in C2C12 cells treated with D-gal and suppressed the decrease in the expression of proliferating cell nuclear antigen. EEPF also markedly blocked D-gal-induced C2C12 cell apoptosis and restored reduced activity of CAT, GSH-Px, T-AOC, SOD. In addition, EEPF suppressed the decrease in 𝛽-galactosidase activity, the induction of DNA damage and the increase in expression of senescence-associated secretory phenotype proteins such as p16, p53 and p21 in D-gal-treated C2C12 cells. Furthermore, EEPF significantly attenuated D-gal-induced production and expression of inflammatory cytokines such as interleukin (IL)-6 and IL-18. Conclusions The results of this study indicate that EEPF can be used as a potential candidate for the prevention and treatment of muscle aging.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.49-55
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• 2009

This paper describes a three-dimensional ground penetrating radar (GPR) survey carried out around a levee of the Ara River in Saitama, Japan, where deformation of the ground was observed after heavy rainfall associated with the typhoon of September 2007. The high-density 3D GPR survey was conducted as a series of closely adjacent four directional sets of 2D surveys at an area surrounding vertical cracks on the paved road caused by deformations induced by heavy rain. The survey directions of the 2D surveys were 0, 90, 45, and -45 degrees with respect to the paved road and the intervals between lines were less than 0.5 m. The 3D subsurface structure was accurately imaged by the result of data processing using Kirchhoff-type 3D migration. As a result, locations and vertical continuities of the heavy rainfall induced cracks in the paved road were clearly imaged. This will be a great help in considering the generation mechanisms of the cracks. Moreover, the current risk of a secondary disaster was found to be low, as no air-filled cavities were detected by the 3D GPR survey.

• Journal of Life Science
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• v.31 no.4
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• pp.400-409
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• 2021

Sanguinarine, a natural benzophenanthridine alkaloid, has been considered a potential therapeutic target for the treatment of cancer because it can induce apoptosis in human cancer cells; however, the underlying mechanisms of action still remain unclear. Tumor suppressor p53 deletion or mutation is an important reason for the resistance of colorectal cancer cells to anticancer agents. Therefore, in the present study, the role of p53 during apoptosis induced by sanguinarine was investigated in p53wild type (WT, p53+/+) and p53null (p53+/+) HCT116 colon carcinoma cells. Sanguinarine significantly caused greater reductions in cell viability in HCT116 (p53+/+) cells than in HCT116 (p53-/-) cells. Consistently, sanguinarine promoted more DNA damage and apoptosis in HCT116 (p53+/+) cells than in HCT116 (p53-/-) cells while increasing the expression of p53 and cyclin-dependent kinase inhibitor p21^WAF1/CIP1. Sanguinarine increased the activity of caspase-8 and caspase-9, which are involved in the initiation of extrinsic and intrinsic apoptosis pathways, respectively, and it activated caspase-3, a typical effect caspase, in HCT116 (p53+/+) cells. Sanguinarine also increased the generation of reactive oxygen species (ROS), and the Bax/Bcl-2 ratio, while destroying the integrity of mitochondria in HCT116 (p53+/+) cells, but not in HCT116 (p53-/-) cells. Overall, the results indicate that sanguinarine induced p53-dependent apoptosis through ROS-mediated activation of extrinsic and intrinsic apoptotic pathways in HCT116 colorectal cancer cells.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Journal of Energy Engineering
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• v.27 no.2
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• pp.1-13
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• 2018

The proportion of natural gas-fueled power generation is expanding due to the change of domestic energy policy pursuing reduction of dust and increasing clean energy consumption. Natural gas fuels used for the combined-cycle power plants and the district-heating power plants are operated at high temperature and high pressure in the fuel supply system. Accidents due to leakage of the gas such as fire and explosion should be prevented by applying risk management techniques. In this study, risk assessment was performed on the natural gas fuel supply system of a combined power plant based on the API RP 581 RBI code. For the application of the API RBI code, lines and segments of the evaluation target system were identified. Operational data and input information were analyzed for the calculations of probability of failure and consequence of failure. The results of the risk assessment were analyzed over time from the initial installation time. In the code-based evaluation, the gas fuel supply system was mainly affected by thinning, external damage, and mechanical fatigue damage mechanisms. As the operating time passes, the risk is expected to increase due to the external damage caused by the CUI(Corrosion Under Insulation).

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.10
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• pp.34-40
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• 2014

With the economical data service delivery, the packet transport network (PTN) technologies have emerged as an important player in the next-generation transport networks. As the PTN continues to make such considerable progress, it is being challenged by network providers who need rapid and reliable recovery capabilities to guarantee the availability of their services. This paper introduces several fault detection mechanisms for a client signal failure in packet transport networks and proposes a reliable transmission method of IP flows from routers using the combination of Ethernet services. Based on the first fault detection methods, client signal fault is detected within tens or hundreds of milliseconds. It enables the client network devices to perform their own recovery processes within one second. The second mechanism enables failed Ethernet services to be bypassed via other Ethernet services over disjoint paths, so as to contribute on reducing packet loss of IP traffic.

• Clean Technology
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• v.27 no.4
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• pp.277-290
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• 2021

A supercapacitor, also called an ultracapacitor or an electrochemical capacitor, stores electrochemical energy by the adsorption/desorption of electrolytic ions or a fast and reversible redox reaction at the electrode surface, which is distinct from the chemical reaction of a battery. A supercapacitor features high specific power, high capacitance, almost infinite cyclability (~ 100,000 cycle), short charging time, good stability, low maintenance cost, and fast frequency response. Supercapacitors have been used in electronic devices to meet the requirements of rapid charging/discharging, such as for memory back-up, and uninterruptible power supply (UPS). Also, their use is being extended to transportation and large industry applications that require high power/energy density, such as for electric vehicles and power quality systems of smart grids. In power generation using intermittent power sources such as solar and wind, a supercapacitor is configured in the energy storage system together with a battery to compensate for the relatively slow charging/discharging time of the battery, to contribute to extending the lifecycle of the battery, and to improve the system power quality. This article provides a concise overview of the principles, mechanisms, and classification of energy storage of supercapacitors in accordance with the electrode materials. Also, it provides a review of the status of recent research and patent, product, and market trends in supercapacitor technology. There are many challenges to be solved to meet industrial demands such as for high voltage module technologies, high efficiency charging, safety, performance improvement, and competitive prices.

• Journal of Wetlands Research
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• v.24 no.4
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• pp.367-376
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• 2022

LID technologies are capable of mitigating the negative impacts of non-point source (NPS) pollution generated in different land uses. Apart from the increase in point and non-point pollutant generation, highly developed and paved areas generally affect microclimate conditions. This study evaluated both the efficiency of Low Impact Development (LID) facilities in treating NPS pollutant loads as well as the unit pollutant loads (UPL) generated in various urban features (such as parking lots and highways). This investigation also looked at how LID technology helped to alleviate Urban Heat Island (UHI) conditions. As compared to the typical unit pollutant loads in South Korea, the unit pollutant loads at Kongju National University were relatively low, because of no classes, limited vehicular transmission, and low anthropogenic activities during vacation. After receiving treatment from the LID facilities, the effluent pollutant loads were significantly decreased. The sedimentation in filtration mechanisms considerably reduced the pollutant fractions in the influent. Additionally, it was shown that LID facilities' mean surface temperatures are up to 7.2℃ lower than the nearby paved environment, demonstrating the LID systems reducing the UHI impact on an urban area.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.237-246
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• 2019

The Electro-Mechanical Brake (EMB) is the next generation braking system for automobiles and railway vehicles. Current brake systems for high-speed trains generate a braking force using a pneumatic cylinder, but EMB systems produce that force through a combination of an electric motor and a gear. In this study, an EMB operation mechanism capable of generating a high braking force was proposed, and structural and vibration analyses of the gears and shafts, which are the core parts of the mechanisms, were performed. Dynamic structural analysis confirmed that the maximum stress in the analysis model was within the yield strength of the material. In addition, the design that maximizes the diameter of the motor shaft was found to be advantageous in strength, and large shear stress could be generated in the bolt fixing the gear and eccentric shaft. In addition, a test apparatus that can reproduce the mechanism of the analytical model was fabricated to measure the strain of the fixed bolt part, which is the most vulnerable part. The strain measurement results showed that the error between the analysis and measurement was within 10%, which could verify the accuracy of the analytical model.

• Journal of Life Science
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• v.21 no.2
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• pp.194-201
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• 2011

Caudal-related homeodomain proteins play critical roles in intestine development and maintenance from Drosophila to humans. The loss or reduction of CDX1 and CDX2 are known to be associated with colon cancers. It has been well known that colorectal carcinogenesis is associated with serious oxidative stress and that catalase is decreased in colon carcinomas. However, the underlying molecular mechanisms remain elusive. Here, we report that Caudal-related homeodomain proteins positively regulate catalase expression in both Drosophila and humans. We found that Drosophila caudal heterozygotes have a decreased catalase expression and increased ROS generation in the hindgut, and that the overexpression of Caudal increases catalase promoter activity and catalase mRNA levels. We also found that CDX1 and CDX2 up-regulate catalase promoter activity and protein levels in HCT116 cells - human colorectal carcinoma cell lines. The level of catalase protein in several colorectal carcinoma cell lines was associated with CDX1 expression. These results suggest that CDX1 and CDX2 may be involved in intestinal homeostasis and tumorigenesis via regulation of catalase expression.