• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.18 no.2
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• pp.77-80
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• 1982

The maneuverabilities of 240 GT class skipjack pole and liner were studied, based on the maneuvering indices and the data which were obtained from Z test of the experimental ship, the m.s. KWANACKSAN, the training ship of the National Fisheries University of Busan. The maneuvering indices K' of the experimental ship were 0.611(at 10$^{\circ}$Z test), 0.622(at 20$^{\circ}$Z test) and 0.556(at 30$^{\circ}$Z test). Her maneuvering indices T' were 1.094(at 10$^{\circ}$Z test), 1.695(at 20$^{\circ}$Z test) and 1.367(at 30$^{\circ}$Z test). The above calculated values K', T' showed that her turning ability at helm angle 20$^{\circ}$ and her obeying ability at helm angle 10$^{\circ}$ were most effective. As it appeared that the calculated K'-values of the experimental ship were smaller than the standard K'-values of different kinds of ships and her overshoot angles at her Z test were greater than those of them, her turning ability was found to be lower. When the experimental ship took a turn at her 10$^{\circ}$Z test, running distance was about 8.3 times her own length and didn't exceed the standard maneuvering distance, 5 to 11 times ship's own length, therefore she was considered to have good maneuverabilities synthetically.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.93-98
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• 2005

Total tocopherol and phenol contents were determined for virgin and pure oilve oil, and their autoxidation and thermal oxidation were compared with those of commercial soybean oil, in the presence or absence of fluorescent light. The total tocopherol contents of virgin, pure olive oil, and soybean oil were 15.7, 11.2, and 80.7 mg/100 g, respectively. Their total phenol contents were 10.4, 1.6 and 0.5 mg/l00 g, respectively. In autoxidation under dark place at 45$^{\circ}C$, the oxidative stability of the substrate oils decreased in order of virgin oilve oil, pure olive oil, and soybean oil. The average temperature coefficients of the virgin, pure olive oil and soybean oil in the range of 45∼$65^{\circ}C$ were 1.73, 1.83 and 1.64, and the activation energies were 26.86, 29.49, and 24.07 KJ/mol, respectively. In temperature range of 45∼$65^{\circ}C$, pure olive oil was the most susceptible to temperature change, whereas soybean oil the least. In autoxidation under fluorescent light at 45$^{\circ}C$, the oxidative stability of substrate oils decreased in the order of soybean oil, pure olive oil, and virgin olive oil. In thermal oxidation at 18$0^{\circ}C$, the oxidative stability of substrate oils decreased in order of pure olive oil, virgin olive oil, and soybean oil.

• Development and Reproduction
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• v.4 no.1
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• pp.125-131
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• 2000

This study has used differential display-PCR, to amplify genes transcribed during the ovarian maturation induced by human chorionic gonadotropin (hCG). The cDNA expressed at the times of acquisition of oocyte maturational competence in red seabream (Pagrus major) following treatment with hCG was amplified and cloned. A full-length of cDNA for p. major was isolated using differential display-PCR and 5'RACE. This cDNA clone contained 2,662 nucleotides including the open reading frame that encoded 434 amino acids. Homology analyses, using the GenBank and EMBL general database searches, indicated that the nucleotides sequence of the cDNA does not have high homology with any other genes. This cDNA was judged to be a gene, which induction of maturational competence coincides with increase of mRNA related ovarian maturation. Consensus sequences which were consistent with protein kinase C phosphorylation sites and casein kinase II phosphorylation sites were identified. in vitro, the transcription level of mRNA related ovarian maturation increased between 9hr and 24hr following treatment of ovarian follicles with hCG. It was also increased after GtH-II (300 ng/ml) stimulation. Furthermore, in vivo, mRNA related ovarian maturation was rarely expressed prior to the acquisition of oocyte maturational competence, but was strongly expressed after the acquisition of oocyte maturational competence, suggesting that the hCG induction of maturational competence is brought about by the de novo synthesis of the mRNA related ovarian maturation in p. major.

• Journal of Life Science
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• v.24 no.11
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• pp.1168-1173
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• 2014

The homogentisate 1,2-dioxygenase (HGD) gene, which consists of 14 exons and spans approximately 42630bp on Bos taurus autosome 1 (BTA 1), is one of the six enzymes required for catabolism of the aromatic amino acids tyrosine and phenylalanine. It has been reported that BTA1 harbors quantitative trait loci that effect marbling score (MS), carcass weight (CW), and longissimus muscle area (LMA) in cattle. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) in the HGD gene and to analyze their association with economic traits in Korean cattle (Hanwoo). Genetic polymorphisms were screened by direct sequencing, which detected 10 SNPs (T11187C, T11301A, T11398G, G29833A, G34256T, G34257C, T34284C, T42333G, T42348C, and T42468C). Six polymorphic sites were selected for genotyping, and economic traits were analyzed using a general linear model in Korean cattle (n=90). The observed genotype frequencies for G34256T were 0.5843(GG), 0.3708(GT), and 0.0449(TT). In addition, 0.3596(GG), 0.3708(GC), and 0.2697(CC) were observed for the G34257C mutation. Statistical association analysis revealed that G34256T polymorphisms were significantly associated with MS, and G34257C polymorphisms were significantly associated with MS and LMA (p<0.05). Further study is needed in order to use the genetic variant as a marker for marker-assisted selection in Korean cattle.

• Biomedical Science Letters
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• v.9 no.4
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• pp.177-181
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• 2003

The baculovirus/Spodoptera frugiperda (Sf) cell system has become popular for the production of large amounts of the human erythrocyte glucose transporter, GLUT1, heterologously. However, it was not possible to show that the expressed transporter in insect cells could actually transport glucose. The possible reason for this was that the activity of the endogenous insect glucose transporter was extremely high and so rendered transport activity resulting from the expression of exogenous transporter very difficult to detect. Sf21-AE cells are commonly employed as the host permissive cell line to support the baculovirus AcNPV replication and protein synthesis. The cells grow well on TC-100 medium that contains 0.1 % D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, unlike the human glucose transporter, very little is known about properties of the endogenous sugar transporter(s) in insect cells. Thus, the uptake of 2-deoxy-D-glucose (2dGlc) by Sf21-AE cells and the inhibition of 2dGlc transport in the insect cells by fructose and cytochalasin B were investigated in the present work. The binding assay of cytochalasin B was also performed, which could be used as a functional assay for the endogenous glucose transporter(s) in the insect cells. Sf21-AE cells were infected with the recombinant virus AcNPV-GT or no virus, at a multiplicity of infection (MOI) of 5. Infected cells were resuspended in PBS plus and minus 300 mM fructose, and plus and minus 20 $\mu$M cytochalasin B for use in transport assays. Uptake was measured at 28$^{\circ}C$ for 1 min, with final concentration of 1 mM deoxy-D-glucose, 2-[1,2-$^3$H]- or glucose, L-[l,$^3$H]-, used at a specific radioactivity of 4 Ci/mol. The results obtained demonstrated that the sugar uptake in uninfected cells was stereospecific, and was strongly inhibited by fructose but only poorly inhibitable by cytochalasin B. It is therefore suggested that the Sf21-AE glucose transporter has very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter.

• Korean Journal of Poultry Science
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• v.31 no.4
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• pp.255-263
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• 2004

Two experiments were conducted to evaluate the feeding values of sesame oil meal (SOM) and to investigate the effects of its dietary supplementation on egg production in laying hens. In experiment I, the values of true metabolizable energy (TME), nitrogen corrected true metabolizable energy (TMEn) and true amino acid availability (TAAA) were determined by force-feeding 16 ISA-Brown roosters and collecting the total excreta from the birds, The TME and TMEn of SOM were 2.30 and 1.99 kcal/g, respectively, and the average TAAA of 15 amino acids was $76.93\%$. In experiment 2, a total of ninety, 48 weeks old ISA-Brown layer were randomly divided into 9 groups of 10 birds each and assigned to three experimental diets containing 0, 5 and $10\%$ SOM for 4 weeks (30 birds per treatment). The inclusion of SOM into laying hen diets at the 5 and $10\%$ level did not affect production and quality of egg. The C18:3 $\omega$3 content of egg yolks in the $10\%$ SOM group was higher than the other groups, but not significantly. There were no adverse effects on blood parameters in layers fed treated diets containing $5\%$ or $10\%$ SOM, The results indicate that SOM can be used for layers diet up to $10\%$ without any significant negative effects on egg production and quality.

• Korean Journal of Microbiology
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• v.28 no.2
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• pp.91-97
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• 1990

Complementary DNA (cDNA) coding for human cytoplasmic superoxide dismutase (SOD1) (superoxide: superoxide oxidoreductase E.C.1.15.1.1) was isolated from human liver cDNA library of $\lambda$gt11 by in situ plaque hybridization. The insery cDNA gas the 5' untranslational region (UTR) and 3'UTR of SOD1 gene. Polymerase Chain Reaction (PCR) method was used fro subcloning of SOD1 structural gene. Using synthetic sense strand primer (24mer) containing a start codon and antisense strand primer (24mer), SOD1 structural gene was selectively amplified. Amplified DNA was directly cloned into the HincII site of pUC19 plasmid. Insery cDNA was subcloned into M13 mp19 and sequenced by dideowy chain termination method with Sequenase. The nucleotide sequence of insert cDNA had an open reading frame (ORF) coding for 153 amino acid residues. The structural gene of cytoplasmic SOD was placed under the control of bacteriophage $\lambda P_{L}$ regulatory sequences, generating a highly efficient expression plasmid. The production of human SOD1 in E. coli cells was about 7% of total cellular proteins and recombinant human SOD1 possessed its own enzymatic acitivity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.677-683
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• 2016

In urban areas, underground tunnel construction sites have spread widely to accommodate rapidly increasing traffic volume along with a high-degree economic growth. Earth tunneling might be adapted frequently for the underground space securing, and various tunneling methods have been developed to stabilize the tunnel face and crown. Among them, the DSM (divided shield method) is gaining popularity for its enhanced stability and construction efficiency. This method has its foundation from the Messer Shield method, which is one of the trenchless special tunneling methods. This study examined the effects of face reinforcement on construction the sequence through a large scale soil chamber test and numerical analyses. The chamber has a size of a 1/2 scale of the real tunnel. Surface settlements were measured according the tunneling process. Commercially available software, MIDAS GTS, was used for numerical analysis and its result was compared with the values obtained from the chamber test. The results of the study show that both settlements of the embanked soils and the stress of the tunnel girder are located within the safe criteria. Overall, this study provides basic data and the potential of using a reinforced tunnel face to enhance DSM applications.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.8
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• pp.3353-3359
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• 2011

The co-generation system consisted of gas a turbine, a steam turbine, heat recovery steam generator and a heat exchangers for district heating was investigated in the present study. A back-pressure steam turbine (non-condensing type) was used. A partial load analysis according to the outdoor temperature in winter was conducted and optimal thermal load and power conditions was examined using the commercial computing software Thermoflex. As a result, under a constant thermal load, the power outputs of gas turbine and overall system increased as an outdoor temperature decreased. On the other hand, the reduction in exhaust gas temperature led to the decrease in output of steam turbine. Considering the portion of gas turbine in overall system in terms of the power output, it can be known that the tendency in power output of overall system was similar to that of the gas turbine.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.1
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• pp.1-5
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• 1987

The authors carried out an experiment to determine the vertical opening of the midwater trawl, which is the same used in the former experiment in this series of studies. To determine the vertical opening of otter board and front weight, three fish finders were used. A 200 KHz fish finder set on board the research vessel was used to sound the depth of water. A transmitter of 50 KHz fish finder was set through the shoe plate of otter board to determine the height of otter board from the sea bed, and a transmitter of another 50 KHz fish finder was set downwardly on the net pendant right before the front weight to determine the height of weight from the sea bed. The depth of otter board and weight were calculated by subtract the height of those from the depth of water, respectively. To determine the vertical opening of mouth, a transmitter of net recorder was set on the head rope and the vertical opening of that to ground rope was directly read on the recording paper. The results obtained can be summarized as follows: 1. The rate of the depth of otter board to the length of warp was in the range of 0.44 to 0.25, and the depth was linearly shoaled about 5m per 0.1m/sec of the towing speed or per 20rpm of the main engine. The rate of the observed depth to the calculated depth of otter board was in the range of 0.92 to 0.080 with a decreasing tendancy in accordance with the increase of towing speed. 2. The depth of head rope was 2 to 3m deeper than that of otter board, and the vertical opening of net mouth was in the range of 22 to 19m, with a decreasing tendancy in accordance with the increase of towing speed, 3. The difference of depth between front weight and otter board was about 20m and 22m respectively in the length of warp 100m and 150m without distinct change in accordance with the towing speed. The depth of front weight was 2 to 3m shallower than that of ground rope. 4. The changing range of depth of head rope according to the revolution of main engine was about 4m per 20rpm.