• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2949-2953
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• 2012

The glutathione S transferase (GST) family is a major part of cellular defense mechanisms against endogenous and exogenous substances, many of which have carcinogenic potential. Alteration in the expression level or structure of the glutathione-S-transferase (GST) enzymes may lead to inadequate detoxification of potential carcinogens and consequently contribute to cancer development. A member of the glutathione-S-transferase (GST) family, GSTP1, is an attractive candidate for involvement in susceptibility to carcinogen-associated colorectal cancer. An $Ag{\rightarrow}G$ transition in exon 5 resulting in an Ile105Val amino acid substitution has been identified which alters catalytic efficiency. The present study investigated the possible impact of Ile105Val GSTP1 polymorphism on susceptibility to colorectal cancer. in Jordan We examined 90 tissue samples previously diagnosed with colorectal carcinoma, and 56 non-cancerous colon tissues. DNA was extracted from paraffin embedded tissues and the status of the GSTP1 polymorphism was determined using a polymerase chain reaction restriction fragment length polymorphism (RFLP) method. No statistically significant differences were found between colorectal cancer cases and controls for the GSTP1 Ile/Ile, Ile/Val and Val/Val genotypes. The glutathione S-transferase polymorphism was not associated with risk in colorectal cancer cases in Jordan stratified by age, sex, site, grade or tumor stage. In conclusion, the GSTP1 Ile105Val polymorphism is unlikely to affect the risk of colorectal cancer.

• 한국콘텐츠학회논문지
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• 제17권10호
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• pp.289-299
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• 2017

아시아인종에서 만성골수성백혈병 (Chronic myeloid leukemia; CML)과 Glutathione S-transferase(GST) 유전자 다형성과 관련된 감수성을 검증하기 위해, 2017년 7월까지 발표된 9편의 논문들을 메타분석에 인용하였다. GST 유전자 다형성의 아형 중 M1 (GSTM1)과 T1 (GSTT1)의 유전자의 null, present 유형을 개별적으로 분석하였다. CML환자와 GST 유전자 다형성 사이에 연관성이 발견되었다.(GSTM1; OR=1.306, 95% CI=1.091-1.563, p=0.004, GSTT1; OR=1.987, 95% CI=1.438-2.746, p=0.000). 또한, CML 환자와 GSTM1-GSTT1 유전자 다형성 조합 null 유형의 연관성이 있었다(OR=4.191, 95% CI=2.833-6.201, p=0.000). 이와 같이, 아시아인종에서 GSTM1 유전자 다형성, GSTT1 유전자 다형성, GSTM1-GSTT1 유전자 다형성 조합은 CML 환자의 위험인자가 될 수 있다.

• 한국콘텐츠학회논문지
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• 제17권7호
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• pp.648-663
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• 2017

아시아인종에서 GSTM1/GSTT1 유전자 다형성과 자가면역질환과 관련된 감수성을 검증하기 위해, 2015년 12월까지 EMBASE, Google, KISS, MEDLINE, PubMed에 발표된 18편의 논문들을 메타분석에 인용하였다. GSTM1/GSTT1 유전자의 null, present 유형을 개별적으로 분석하였다. 전체 인구에서 GST 다형성과 자가면역질환 사이에 연관성이 발견되었다(GSTM1, OR=1.334, 95% CI=1.137-1.567, p=0.000, GSTT1, OR=1.212, 95% CI=1.012-1.452, p=0.037). 아시아인종에서 자가면역질환, 특히 vitiligo와 아토피 피부염(p<0.05)에서 GSTM1 유전자와의 연관성이 있었고, RA와 SLE에서 GSTT1 유전자와의 연관성은 없었다(p>0.05). GSTM1 null 유형과 GSTT1 present 유형은 아시아인종에서 자가면역질환과 연관이 있었다(p<0.05). 자가면역질환과 GSTM1-GSTT1 다형성 조합 null 유형의 빈도는 대조군보다 높았다. 이와 같이, GSTM1-GSTT1 다형성 조합 null 유형이 아시아인종에서 자가면역질환의 위험 인자가 될 수 있다.

• 대한한방내과학회지
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• 제28권3호
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• pp.453-463
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• 2007

Backgrounds : Glutathione-s-transferase (GST) is a kind of phase II metabolism enzyme and plays an important role in the detoxification of various toxic chemicals. It was reported that the genetic polymorphism of GSTM1 and GSTT1 genes may be responsible for asthma development and susceptibility to allergy. Traditional oriental medicine uses a unique diagnostic technique. differentiation-syndrome. to analyze signs and symptoms of patients synthetically. Through differentiation-syndrome. asthma patients can be divided into two groups: the deficiency syndrome group (DSG) and the excess syndrome group (ESG). Objectives : The purpose of this study was to investigate the possible association of GST gene polymorphism with clinical phenotype by differentiation-syndrome of bronchial asthma patients. Materials and Methods : One hundred and ten participants were evaluated by pulmonary function test. Patients with 53 DSG and 31 ESG by differentiation-syndrome were assessed for genetic analysis. GSTM1 and GSTT1 deletion polymorphism was performed by polymerase chain reaction (PCR). Results : GSTM1 gene deletion was detected in 43.4% of individuals in the DSG and in 38.71 % in the ESG. The distribution of GSTM1 polymorphism between DSG and ESG was not significantly different [$x^2$=0.1767, p=0.6742; OR(95% CI)=1.2139(0.4915-2.9979)]. The proportion of GSTT1 null genotypes was 41.51% in the DGS and 45.16% in the ESG. The distribution of GSTT1 polymorphism between DSG and ESG was also not significantly different [$x^2$=0.1065, p=0.7442; OR(95% CI)=0.8618(0.3525-2.1065)]. In the combined analysis of GSTM1 and GSTT1 genes, the frequency of both null type of GSTM1/GSTT1 genes was not significantly different from both positive type of GSTM1/GSTT1 genes[$x^2$=0.0768, p=0.7817; OR(95% CI)=1.2000(0.3303-4.3602)] Conclusions : These results indicate that polymorphism of the GST gene might not be associated with the symptomatic classification of DSG and ESG by differentiation-syndrome in Korean asthmatics.

• 대한한의학회지
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• 제25권4호
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• pp.36-42
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• 2004

Objective : Glutathione S-transferase polymorphism (GST) were examined in 120 cases with ischemic cerebrovascular disease (ICVD) to test the hyperthesis that GST polymorphisms confer a risk to an individual to develop ICVD. Tobacco smoking is a major cause of both cancer and vascular disease. Methods : therefore We were stratified the subjects with ICVD for smoking status, and then examined whether polymorphisms in this detoxification enzyme gene, GST, influence risk of ICVD Results : Neither GSTM1 nor GSTT1 genotypes in the ICVD group was significantly different from the control group (n=207), even in smokers. We attempted the combined analyses for GSTM1 and GSTT1 genotypes in ICVD for smoking status. No significant association observed between the combined genotypes and ICVD Conclusion : Our observation do not confirm the effect of the GSTM1 and GSTT1 genotypes as a risk factor for ICVD, even in smokers.

• 수면정신생리
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• 제22권1호
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• pp.25-29
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• 2015

목 적 : 하지불안증후군(restless legs syndrome ; RLS)의 병인은 아직 불명확하지만, 유전적 질환으로 알려져 있다. 산화스트레스는 RLS, 지연성운동장애, 파킨슨병, 뚜렛장애 등의 운동장애에서 주요한 원인 중의 하나로 생각되고 있다. 본 연구에서는 조현병환자에서 항정신병약물에 의해 유발된 RLS 증상이 산화손상의 해독효소인 glutathione S-transferase (GST) 유전자의 다형성과 연관이 있는지를 밝히고자 하였다. 방 법 : International Restless Legs Syndrome Study Group의 진단기준으로 190명의 한국인 조현병 환자들을 대상으로 RLS에 대해서 평가하였다. 유전자형분석은 중합효소연쇄반응기법을 사용하여 GST-M1, GST-T1, GST-P1의 세 가지 단일염기다형성(single nucleotide polymorphism, SNP)에 대해서 시행되었다. 결 과 : RLS 증상군 96명과 무증상군 94명으로 피험자들을 분류하였다. GST-M1 (${\chi}^2=3.56$, p = 0.059), GST-T1 (${\chi}^2=0.51$, p = 0.476), GST-P1 (${\chi}^2=0.57$, p = 0.821)의 유전자형 빈도에 두 군간에 통계적으로 유의한 차이가 없었다. 유전자형에 따른 RLS 척도의 점수도 GST-M1 (t = -1.54, p = 0.125), GST-T1 (t = -0.02, p = 0.985), GST-P1 (F = 0.58, p = 0.560)의 세 가지 SNP에서 통계적으로 유의한 차이를 보이지 않았다. 결 론 : 본 연구의 결과 GST 유전자 다형성이 항정신병약물로 유발된 RLS 증상 발생의 민감성을 증가시킨다는 증거는 발견할 수 없었다. 산화손상과 관련된 다른 후보 유전자들에 대한 향후 연구가 필요할 것으로 사료된다.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2000년도 제52차 추계학술대회 연제집
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• pp.22-22
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• 2000

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1076-1079
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• 2002

The DNA fingerprint polymorphism and the genetic relationship were studied by RAPD technology on Chaidamu goat (CG), Chaidamu Cashmere goat (CCG) and Liaoning Cashmere goat (LCG) from Chaidamu Basin of Qinghai province, China. The results showed that: The amplified bands were all 94 in 3 goat populations by using 8 random primers, and the DNA polymorphism frequencies of CG, CCG and LCG were 0.8404, 0.8617 and 0.8511, respectively, and the length of these DNA fragments were 176-2937 bp. The mean heterozygosities of the 3 goat populations were 0.5148, 0.5142 and 0.5075, respectively. The genetic relationship between CCG and CG or LCG were similar (Gst=4.37% and 3.79%; $D_{ij}=0.0109$ and 0.0106), and that between CG and LCG was further (Gst=13.14%; $D_{ij}=0.0230$). These results also showed that the genetic relationship between CCG and LCG was the closest, then CG and LCG, and CG and CCG was distant.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1998년도 제50차 추계 학술대회 연제집
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• pp.285-286
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• 1998

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3325-3328
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• 2012

Aim: To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection. Methods: We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP. Results: The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer. Conclusion: GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.