• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2007년도 Proceedings of The Convention of The Korean Society of Applied Pharmacology
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• pp.25-44
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• 2007

The glucocorticoid receptor (GR) is an intracellular protein that is widely distributed throughout hippocampal and neocortical brain tissue. Mifepristone (RU486) is a potent GR antagonist that has also been shown to exhibit partial agonist-like effects. The precise location of the GR domain involved in the agonist-like activity of RU486 is unknown. Here, we examine this aspect of GR signaling by comparing human GR (hGR) construct with a Guyanese squirrel monkey GR (gsmGR) construct in which nuclear translocation and transactivation are known to be impaired. Using an objective translocation scoring method, we found that both hGR and gsmGR are translocated by RU486, and that nuclear translocation of hGR is significantly increased compared to gsmGR at 10 nM, 100 nM and 1000 nM RU486 in transiently transfected COS1 cells. While addition of RU486 to the cells transfected with hGR results in a 16-fold dose-dependent increase in transactivation compared to non-treated cells, no significant change in transactivation is observed with gsmGR at doses up to 100 nM RU486. Further experiments using six GR chimeras indicate that replacement of the hGR carboxyl-terminus of tau-1 transactivation domain (C-AF1, amino acids 132-428) with that from gsmGR diminishes hGR transactivation by RU486. These results demonstrate that RU486-induced transactivation of GR is determined in part by amino acids in the C-AF1 domain.

• 한국임상수의학회지
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• 제30권5호
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• pp.353-358
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• 2013

본 연구는 오배자(Galla Rhois, GR)가 이유자돈의 성장과 설사발생에 미치는 영향을 조사하였다. 100두의 28일령 이유자돈을 무작위적으로 5개의 실험군(NC, 대조군; PC, chlortetracycline 0.3 g/kg feed; GR 1, GR 1.0 g/kg feed; GR 2, GR 2.0 g/kg feed; GR 3, GR 4.0 g/kg feed3)으로 나누어 실험을 수행하였다. 28일 동안 실험군별로 처리한 후, PC, GR 2 그리고 GR 3의 최종 체중과 사료요구율은 NC와 비교하여 통계적으로 유의성 있게 증가하였다(p < 0.05). 또한, PC와 GR를 급여한 모든 군의 일평균증체량과 일평균사료섭취량도 NC와 비교하여 통계적으로 유의한 차이를 보였다(p < 0.05). 특히, GR 2와 GR 3의 최종 체중, 일일평균증체량, 일평균사료섭취량 그리고 사료요구율은 PC와 비교하여 유의한 차이를 보이지 않았다. 분변지수와 설사지속시간에 있어서, PC와 GR를 급여한 모든 군이 NC와 비교하여 통계적으로 유의한 차이를 나타내었다(p < 0.05). 혈액학적 그리고 혈액생화학적 분석 결과, NC와 GR를 급여한 모든 군들 사이에서 혈액학적 그리고 혈액생화학적 지표값들의 통계학적 유의한 차이가 보이지 않았다. 본 연구결과로부터, GR은 자돈에 있어서 설사발생과 성장증체를 개선시키기 위한 강력한 사료첨가제로 사료된다.

• 한국작물학회지
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• 제23권2호
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• pp.141-144
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• 1978

패모린경의 무게를 15gr, 3gr, 5gr, 7gr, 9gr의 5등급별로 나뉘어 파종하였을 때에 그에 따른 발아상태, 초장, 개화, 자구 및 수량에 대하여 조사하였다. 1. 인경의 무게가 1.5gr 및 3gr되는 것은 발아시, 발아기간이 5gr, 7gr, 및 9gr되는 것에 비하여 발아시가 늦고 발아기간이 긴 경향을 나타냈고, 초장도 3∼5분지 1밖에 되지 않었다. 2. 개화는 인경이 무겁고 영양상태가 양호한 7gr∼9gr 상태에서 50％∼75％ 였으나, 3gr이하의 것은 1％에 불과했다. 또한 자구는 5gr이상에서 현저한 증가를 보였다. 3. 수량을 증가시키기 위하여서는 5gr이상의 패모를 사용하여야 한다는 사실을 알게 되었다.

• Molecules and Cells
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• 제40권10호
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• pp.731-736
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• 2017

Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a-Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a-Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a-Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a-Gr64f genes.

• Applied Microscopy
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• 제11권1호
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• pp.39-50
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• 1981

성숙하고 정상인 포유류 6종의 동물들을 이용하여 위에 분포하는 비만세포의 형태와, 세포내 과립의 구조적 형태의 종간차이를 비교하고자 전자현미경으로 관찰을 하였다. 저자는 특히 관찰된 세포질내 과립을 편의상 다음과 같이 분류하였다. 1) 동질성 과립 (homogeneous granule, GR1) 2) 미세 입자과립 (particulate granule, GR2) a. 고전자밀도 미세 입자과립 (dark dense particulate granule, GR2-1) b. 중전자밀도 미세 입자과립 (less dense particulate graunle, GR2-2) 3) 망상구조과립 (reticular granule, GR5) a, 고전자말도 망상구조과립(dark dense reticular granule, GR5-1) b. 저전자밀도 망상구조과립(light dense reticular granule, GR5-2) 포유류인 염소, 개, 고양이, 햄스터에서는 대부분 세포질내 소기관중 골지체 및 사립체가 나타났으며 세포질내 과립의 대부분이 전자밀도가 높은 동질성인 것 (GR1)과 미세입자형으로 된 것 (GR2)이 나타났다. 그러나 Guinea pig에서는 망상구조형의 과립들 (GR5-1, GR5-2)이 나타났다. 또한 개와 Guinea pig 에서 과립의 한쪽이 함몰되었거나 반월형인 것이 나타났다. 위 결과로 보아 표유류 각 종 간의 비만세포, 세포내 과립은 형태학적으로 차이가 있음을 알 수 있으며, 이러한 차이는 아마도 종 간의 기능에 따른 과립의 구조적 차이와 구성성분 및 성숙도등과 상관관계가 있는 것으로 추측된다.

• Journal of Applied Biological Chemistry
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• 제59권3호
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• pp.179-188
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• 2016

본 연구의 목적은 증숙 및 발효과정을 거친 천마추출물이 LPS로 유도된 간 손상에 미치는 효능을 평가하고자 하였다. 증숙 횟수 및 발효균을 달리한 25가지 시료 중 DPPH와 ABTS 라디칼 소거능을 측정하여 생 천마(GR0F0)와 6회 증숙한 후, Saccharomyces cerevisiae균으로 발효한 GR6F4간의 비교실험이 가능할 것으로 사료되었다. 마우스는 6마리씩 4그룹(정상군, 대조군, GR0F0 투여군, GR6F4투여군)으로 나누어 실험을 진행하였다. LPS를 투여하기 전, 각 시료를 200 mg/kg/day로 8일간 경구 투여하였으며, 간 손상을 유발하기 위하여 LPS를 20 mg/kg로 복강투여하였다. 혈액에서 AST, ALT를 측정한 결과, 대조군에서 그 수치가 증가하였고, GR6F4군에서 감소하였을 뿐 아니라, 혈액 및 간에서 측정한 ROS 수치 또한 유의하게 감소하였다. 간조직에서 AP-1, COX-2, TNF-${\alpha}$와 INOS를 측정한 결과, GR0F0 또한 감소하였으나, 증숙 및 발효과정을 거친 GR6F4군에서 더욱 큰 효과를 보이는 것을 확인하였다. 이러한 결과들은 GR6F4의 뛰어난 항산화 및 항염증 효과를 증명하며, 그러므로 증숙 및 발효과정을 거친 천마는 간 손상에 보호효과가 있다고 보여진다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1557-1567
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• 2012

Glutathione reductase (GR, E.C. 1.6.4.2) is an important enzyme that reduces glutathione disulfide (GSSG) to a sulfydryl form (GSH) in the presence of an NADPH-dependent system. This is a critical antioxidant mechanism. Owing to the significance of GR, this enzyme has been examined in a number of animals, plants, and microbes. We performed a study to evaluate the molecular properties of GR (OsGR) from rice (Oryza sativa). To determine whether heterologous expression of OsGR can reduce the deleterious effects of unfavorable abiotic conditions, we constructed a transgenic Saccharomyces cerevisiae strain expressing the GR gene cloned into the yeast expression vector p426GPD. OsGR expression was confirmed by a semiquantitative reverse transcriptase polymerase chain reaction (semiquantitative RT-PCR) assay, Western-blotting, and a test for enzyme activity. OsGR expression increased the ability of the yeast cells to adapt and recover from $H_2O_2$-induced oxidative stress and various stimuli including heat shock and exposure to menadione, heavy metals (iron, zinc, copper, and cadmium), sodium dodecyl sulfate (SDS), ethanol, and sulfuric acid. However, augmented OsGR expression did not affect the yeast fermentation capacity owing to reduction of OsGR by multiple factors produced during the fermentation process. These results suggest that ectopic OsGR expression conferred acquired tolerance by improving cellular homeostasis and resistance against different stresses in the genetically modified yeast strain, but did not affect fermentation ability.

• 한국초등과학교육학회지:초등과학교육
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• 제30권4호
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• pp.535-552
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• 2011

The purpose of this study was to investigate elementary school teachers' conceptions on the general remarks (GR) in the new science teacher's guide, the teachers' conceptions on inservice training program of the GR, and their opinions of improvement on the GR. Also, the teachers' utilization of the GR was examined. The participants were 152 elementary school teachers who were teaching grade 3~6. A questionnaire was used to collect data. The results of this study are as follows; First, at least 13.2~17.1% of the teachers did not have specific conception of the purpose of the GR, and more than 90% of the teachers expressed that the GR is necessary in the teachers' guide. Second, about 60% of the teachers responded that the training program on the GR is necessary, and when the program is open, their most favorite agency and speaker were a provincial education office and a textbook developer(or author), respectively. Their most favorite time and period of the training program were the vacation and 15~30 hours, respectively. Third, the mean values on the frequency of use were lower than 3 point of the five-point Likert scale at both the GR for grade 3~4 and the GR for grade 5~6, and the teachers' main use of the GR was the planning a science lesson for an open class. Fourth, the teachers suggested various opinions and there was difference between the opinions about the GR for grade 3~4 and the opinions about the GR for grade 5~6.

• Nuclear Engineering and Technology
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• 제56권1호
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• pp.1-8
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• 2024

In this study, the influence of thermal aging on structural integrity is investigated for Gr. 91 steel. A commercial grade Gr. 91 steel is used for the virgin material, and service-exposed Gr. 91 steel is sampled from a steam pipe of a super critical plant. Time versus creep strain curves are obtained through creep tests with various stress levels at 600 ℃ for the virgin and service-exposed Gr. 91 steels, respectively. Based on the creep test results, the improved Omega model is characterized for describing the total creep strain curve for both Gr. 91 steels. The proposed parameters for creep deformation model are used for predicting the steady-state creep strain rate, creep rupture curve, and stress relaxation. Creep-fatigue damage is evaluated for the intermediate heat exchanger (IHX) in a large-scale sodium test facility of STELLA-2 by using creep deformation model with proposed creep parameters and creep rupture curve for both Gr. 91 steels. Based on the comparison results of creep fatigue damage for the virgin and service-exposed Gr. 91 steels, the thermal aging effect has been shown to be significant.

• Korean Journal of Mathematics
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• 제20권3호
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• pp.315-321
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• 2012

In this paper, we provide the necessary and sufficient conditions for a faithful graded module to be a graded multiplication module and for a graded submodule of a faithful gr-multiplication to be gr-essential.