• KSBB Journal
• /
• v.23 no.6
• /
• pp.504-508
• /
• 2008

The objectives of this study were to develope the economical process for bioethanol production from domestic triticale and investigate optimal fermentation conditions such as temperature, time, and enzyme concentration used to pre-treatment process. Triticale mash, containing 148 g of total sugar per 1 L of mash, was fermented with Saccharomyces cerevisiae CHY1011 at $33^{\circ}C$. Fermentation of mash supplemented with enzyme was completed within 48-60 hours, and the ethanol yield was 410.9 L/tonne of dry base. On the other hand, fermentation of mash without enzyme addition was completed within 36-48 hours, but the ethanol yield was 342.2 L/tonne of dry base. For optimal bioethanol production from triticale, viscosity reduction enzyme was added in the pre-treatment process, and the fermentation rate of triticale was 92.0-94.2%. In addition, the results showed that bioethanol production of triticale by low-temperature pre-treatment would provide higher ethanol production efficiency and lower operating costs.

• Journal of the Korean Society of Food Culture
• /
• v.20 no.1
• /
• pp.123-129
• /
• 2005

The purpose of this study was to investigate the effect of dietary Morus alba L.(Bong-ip, B), Glycyrrhizae glabra(Gam-chei, C), Pinus densiflora(Sol-lp, S) and Angelica gigas(Dang-gi, D)powder on serum composition in rats(Sprague-Dawley male rats, 100-110g). Serum TG(triglyceride, p<0.01), total cholesterol, glucose, total protein, albumin, GGT$({\gamma}-glutamyl$ transferase, p<0.05) were significantly increased D group than that of nomal and other groups, but UA(uric acid, p<0.05) was significantly decreased, and C group(p<0.05) was significantly increased. but C group of urine(p<0.05) was significantly decreased. Also, B and S groups(p<0.05) of BUN(blood urea nitrogen), S group(p<0.05) of ALP(alkaline phosphokinase, Band C(p<0.05) of CPK(creatinine phosphokinsae, p<0.05) were significantly increased. B, S and C groups were better than D group for lipid metabolism, and pretection to liver. Also, B and C groups of glucose were same as normal diet, so Morus alba L. was good food for lipid metabolism and hypoglycemic effect.

• The Korean Journal of Physiology and Pharmacology
• /
• v.13 no.5
• /
• pp.393-400
• /
• 2009

NO released by myenteric neurons controls the off contraction induced by electrical field stimulation (EFS) in distal esophageal smooth muscle, but in the presence of nitric oxide synthase (NOS) inhibitor, L-NAME, contraction by EFS occurs at the same time. The authors investigated the intracellular signaling pathways related with G protein and ionic channel EFS-induced contraction using cat esophageal muscles. EFS-induced contractions were significantly suppressed by tetrodotoxin ($1\;{\mu}M$) and atropine ($1\;{\mu}M$). Furthermore, nimodipine inhibited both on and off contractions by EFS in a concentration dependent meaner. The characteristics of 'on' and 'off contraction and the effects of G-proteins, phospholipase, and $K^+$ channel on EFS-induced contraction in smooth muscle were also investigated. Pertussis toxin (PTX, a $G_i$ inactivator) attenuated both EFS-induced contractions. Cholera toxin (CTX, $G_s$ inactivator) also decreased the amplitudes of EFS-induced off and on contractions. However, phospholipase inhibitors did not affect these contractions. Pinacidil (a $K^+$ channel opener) decreased these contractions, and tetraethylammonium (TEA, ${K^+}_{Ca}$ channel blocker) increased them. These results suggest that EFS-induced on and off contractions can be mediated by the activations Gi or Gs proteins, and that L-type $Ca^{2+}$ channel may be activated by G-protein ${\alpha}$ subunits. Furthermore, ${K^+}_{Ca^-}$ channel involve in the depolarization of esophageal smooth muscle. Further studies are required to characterize the physiological regulation of $Ca^{2+}$ channel and to investigate the effects of other $K^+$ channels on EFS-induced on and off contractions.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.12
• /
• pp.1633-1639
• /
• 2009

Water extracts were extracted on water bath for 3 hrs at 90${^{\circ}C}$ after 5 times water was added to hot-air dried garlic (DG) and red ginseng (RG), respectively. Its extracts were dried on rotary evaporator, and then the mixture samples were prepared from RG extracts added to DG extracts at 5, 10, and 15%. To test biological activities such as DPPH, nitrite scavenging, tyrosinase, $\alpha$-glucosidase, human gastric (AGS), and human colon cancer cell (HT-29) growth, dryness of DG, RG, and mixture samples were diluted 500-10,000 $\mu$g/mL adding deionized water, respectively. DPPH scavenging activities of mixture sample were slightly higher than DG and RG sample alone. Nitrite scavenging activity was the highest in DG, and the other samples were below 45%. Inhibition activity of tyrosinase was below 35.0% in tested all samples. Inhibition activity of $\alpha$-glucosidase was lower in DG or RG alone, while its activity of mixture sample was increased in proportion to concentration of RG extracts. Inhibition of AGS cell growth was more effective in mixture samples than DG and RG alone, while inhibition of HT-29 cell growth was more effective in DG or RG alone than mixture samples.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.26 no.2
• /
• pp.69-76
• /
• 2006

To optimize tissue culture responses for genetic transformation of Kentucky bluegrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of a cultivar 'Newport' as explant tissues. The optimal concentration of 2,4-D (2.4-dichloro phenoxy acetic acid) for the induction of embryogenic callus from mature seed was 3 mg/L. Plant regeneration frequency was 54% when embryogenic callus was cultured on the regeneration medium supplemented with 1 mg/L 2,4-D and 3 mg/L of BA (6-benzyladenine). Addition of 1 g/L of casein hydrolysate and 500 mg/L of L-proline improved frequencies of embryogenic callus induction and plant regeneration up to 60.8% and 58.3%, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study. We suggest that the results may be useful for molecular breeding of Kentucky bluegrass through genetic transformation.

• Journal of Korean Philosophical Society
• /
• v.117
• /
• pp.355-372
• /
• 2011

G. Deleuze a $exerc{\acute{e}}$ sa philosophie $cin{\acute{e}}mathographique$ dans ses œuvres essentielles $Cin{\acute{e}}ma$ 1: l'Image-Mouvement(Minuit:1983) et $Cin{\acute{e}}ma$ 2: l'Image-Temps(Minuit:1985) qui n'indiquent pas l'histoire $cin{\acute{e}}mathographique$ mais la taxinomie, et par $cons{\acute{e}}quent$, de l'image et du code. Cette ${\acute{e}}tude$ consiste donc $\grave{a}$ rendre compte la de l'image que G. Deleuze touche dans cette $d{\acute{e}}finition$ et $\grave{a}$ $v{\acute{e}}rifier$ quelles images peuvent ${\hat{e}}tre$ $cat{\acute{e}}goris{\acute{e}}es$: comment. Nous distinguons l'image-mouvement et l'image-temps qui sont les $cat{\acute{e}}gories$ essentielles chez $Cin{\acute{e}}ma$. C'est surtout sur ce point que nous insistons. Dans ce cas, nous discutons aussi de la question des spectateurs du film qui conduit. Cette question permet susciter de la $mani{\grave{e}}re$ $d^{\prime}{\hat{e}}tre$ des spectateurs entre le film qui est $compos{\acute{e}}$ de l'image-mouvement dont G. Deleuze decrit comme 'classique' et le film qui est $trait{\acute{e}}$ de l'lmage-temps dont il $d{\acute{e}}finit$ comme 'moderne': quelle variation. Dans ce point $d^{\prime}{\acute{e}}tude$, G. Deuleuze ecrit cette question $mentionn{\acute{e}}e$ $\grave{a}$ travers son oeuvre $Cin{\acute{e}}ma$($\grave{a}$ voir ch.7, Volume2).

• Korean Journal of Food Science and Technology
• /
• v.36 no.6
• /
• pp.1001-1007
• /
• 2004

Chloroform layer from methanol extract of Corni fructus (Cornaceae) showed strong antiproliferation effect on human cancer cell lines by SRB assay. Anticarcinogenic-active compound was isolated and purified by silica gel column and thin layer chromatograpies, and identified as ursolic acid ($3{\beta}$-hydroxyrus-12-ene-28-oic acid, MW:456) by mass and IR spectrophotometries, and $^1H-and\;^{13}C-NMRs$. The compound inhibited proliferation of A549 (human lung cancer cell line) and MCF-7 (human breast cancer cell line) cells in dose-dependant manner when treated for 48 hr. Inhibition rates of both cells were over 40% and 90% compared with control cells at the $30\;{\mu}g/mL\;and\;100\;{\mu}g/mL$, respectively. Morphology of cells treated with the compound for 15 hr at $10\;{\mu}g/mL$ was distorted with shrinked cell mass, and cell number was lower than that of control cells. Cell cycle analysis showed sub-G1 phase arrest in both cell lines following 15 hr exposure to the compound; % of cell phase increased to 11.7 and 11.2% compared to the control of 4.0% and 2.1% in A549 and MCP-7 cells, respectively.

• YAKHAK HOEJI
• /
• v.36 no.5
• /
• pp.491-495
• /
• 1992

For the research of cytotoxic natural products, 50 medicinal plants were extracted with benzene and methanol, separately, and screened against L1210 cells. From the results(Table I), 6 samples showed cytotoxicity both in benzene and methanol extracts of 17 samples in benzene extracts and 3 samples in methanol extracts, respectively. Generally, the cytotoxicity exhibited high frequency (34%) in benzene extract but low frequency in methanol extract (6%), it meant that active cytotoxic components had less polarity. $ED_{50}$ values less than $10\;{\mu}g/ml$ were observed in 17 medicinal plants.

• Journal of Korean Society of Environmental Engineers
• /
• v.27 no.11
• /
• pp.1228-1237
• /
• 2005

In order to predict the long-term effects of pollutants in sediment on the water quality and the improvement of water quality according to dredging operation, models applied to decide the location and the propriety of dredging were developed. At first, the area was divided into several segments and the developed model was applied to simulate the behaviors of contaminants in an aquatic environment by using estimated parameters. And then through the sensitivity analysis between parameters, the optimum values were determined. The long-term modelling in the area A forecasted that PCBs concentration in the hot spot was decreased from $3.1\;{\mu}g/L$ to $2.4\;{\mu}g/L$ in 30 years. Contaminants in sediment as a source of water pollution did not reduce remarkably in the long run. Therefore it is difficult to expect the improvement of water qualities without the fundamental isolation of contaminants from sediment. It is considered that the selective dredging in the spot improves the water quality consequently.

• Fisheries and Aquatic Sciences
• /
• v.2 no.2
• /
• pp.182-188
• /
• 1999

Production of extracellular polysaccharide by Monilinia fructigena in B-I medium containing cereals was higher than that in glucose medium. Productivities in B-I medium and glucose medium were 0.7g/l nd 0.2-03g/l respectively. The maximum content of polysaccharide occurred at the rising point from the lowest pH of culture. As the apparent viscosity of the polysaccharide solution increased, the flow Index (m) decreased, and the consistency Index (Kc) also increased. The polysaccharide solution was a typical pseudoplastic fluid. The mycelium was separated from the culture solution by $300\mu m$ mesh-filter and the polysaccharide was precipitated by adding 50% of ethanol (v/v). The amount of the polysaccharide removed from the filtrated solution was 0.45 g/l and the amount adhered to the mycelium was 0.25g/l. In experiments for investigating growth enhancement of rotifer (Brachionus plicatilis) by the polysaccharide, the dose of the polysaccharide was 1mg per 10,000 organisms of rotifer. Maximum specific growth rate of rotifer with feed consisting of sea Chlorella sp. and the polysaccharide was 1.095/day in the batch culture for 10 days. A semi-continuous culture was done for 30 days, the biomass of rotifer could be harvested twice. Maximum specific growth rate with sea Chlorella sp. and the polysaccharide was 0.734/day before the first harvest, and 1.685/day before the second harvest. Productivity was 38 $cells/ml\; \cdot\; day$ with sea Chlorella sp. and the polysaccharide.