• Title/Summary/Keyword: GI/G/l

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Production of DFAIII by Fermentation and Enzyme Reaction and its Recovery (발효 및 효소반응을 통한 DFAIII의 생산 및 회수)

  • Lee, Jae-Chan;Lee, Gi-Yeong;Song, Gi-Bang;Lee, Yong-Bok
    • KSBB Journal
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    • v.14 no.5
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    • pp.539-542
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    • 1999
  • For the mass production of DFAIII and for the development of techniques of separation and purification of it, the methods of production of DFAIII and its recovery was investigated by fermentation with the strain of Arthrobacter ureafaciens KCTC 3387 and by enzyme reaction. In the first method, DFAIII was produced by fermentation with the strain of Arthrobacter ureafaciens KCTC 3387 and recovered from culture supernatant with silica gel gy filtration, in the second method, it was produced by enzyme reaction and recoverd with the same method of the first, and in third method it was produced by fermentation and recovered by addition of ethanol to the culture supernatnat.Against 25g/L of initial concentration of inulin, 1.57, 4.40, 0.34 g/L of powder of DFAIII was recovered respectively and the rate of recovery was 6.3, 17.6 1.4% and the purity was estimated at 81, 97, 87% respectively. For the production of DFAIII and its recovery, enzyme reaction method was the highest in the rate of recovery and its purity. By fermentation method, DFAIII was produced with 50% fo initial concentration of substrate but th rate of recovery was lower than enzyme reaction method and purity was lowest among the three methods. Ethanol pricipitation method showed the lowest rate of recovery.

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Effect of Saline Concentrations on Biological Nitrification in Batch Reactor

  • Lee, Young Joon;Nguyen, Viet Hoang;Nguyen, Hong Khanh;Pham, Tuan Linh;Kim, Gi Youn
    • Journal of Integrative Natural Science
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    • v.4 no.2
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    • pp.103-112
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    • 2011
  • This study was carried out on 4 batch reactors to determine the specific ammonium oxidizing rate (SAOR), specific nitrate forming rate (SNFR) and inhibitory degree of nitrifying activities with saline concentrations. Under salt free condition ammonia was consumed during the reaction period within 200 min. When the salt level increased to 10, 20 and 30 g $NaClL^{-1}$ in reactor, ammonia depletion took 250, 300 and above 350 min, respectively. During concentration above 10 g $NaClL^{-1}$, there was nitrite accumulation. Also, at 30 g $NaClL^{-1}$ ammonia did not depleted and $NO_2{^-}$-N accumulated until the final reaction. Nitrate formation rates decreased with increasing salt concentration. SAOR and SNFR showed a decreasing trend as salinity concentrations were increased. The SAOR was reduced from 0.2 to 0.08 mg $NH_4{^+}$-N $g^{-1}VSS\;day^{-1}$ as the salt concentration increased from 0 to 30 g $NaClL^{-1}$. Similarly, the SNFR decreased from 0.26 kg $NO_3{^-}$-N $kg^{-1}VSS\;day^{-1}$ at saline free to 0.1 kg $NO_3{^-}$-N $kg^{-1}VSS\;day^{-1}$ at saline 30 g L-1. A severe inhibition of nitrifiers activity was observed at increased salt concentrations. The inhibition ratio of specific ammonium oxidation rates were 17, 47 and 60% on the reactor of 10, 20 and 30 g $NaClL^{-1}$ added, respectively. The inhibition ratio of specific nitrate forming rates also were inhibited 30, 53 and 62% on the reactor of 10, 20 and 30 g $NaClL^{-1}$ added, respectively. As the salinity concentrations increased from 0 to 30 mg $NaClL^{-1}$, the average MLSS concentration increased from 1,245 to 1,735 $mgL^{-1}$. The SS concentration of supernatant in reactor which settled about 30 minutes was not severely difference between concentration of salt free reactor and one of those high salt contained reactors.

Pilot Scale Production of Poly (3-Hydroxybutyrate-co-3-hydroxy-valerate) by Fed-batch Culture of Recombinant Escherichia coli

  • Park, Jong-il;Lee, Sang-Yup;Kyungsup Shin;Lee, Woo-Gi;Park, Si-Jae;Chang, Ho-Nam;Chang, Yong-Keun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.6
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    • pp.371-374
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    • 2002
  • Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)[P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhy-droxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only, In a 30 L fermentor having a XLa value of 0.11 S­$^1$, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a XLa of 0.03 S­$^1$, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively giving a productivity of 1.06g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

The Algicidal Effect of Antimicrobial Peptide, Mastoparan B (항균성 펩타이드인 mastoparan B의 살조효과)

  • Seo, Jeong-Gil;Kim, Chan-Hui;Bae, Yun-Jeong;Mun, Ho-Seong;Kim, Geun-Yong;Park, Hui-Yeon;Yun, Ho-Dong;Kim, Chang-Hun;Byeon, Dae-Seok;Hong, Yong-Gi;Park, Nam-Gyu
    • Journal of fish pathology
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    • v.16 no.3
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    • pp.193-201
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    • 2003
  • Mastoparan B (MPB), an antimicrobial cationic peptide isolated from the venom of the hornet Vespa basalis, is a basic amphipathic α-helical peptide composed of fourteen amino acid residues. In this study, we have investigated the algicidal effect of MPB against harmful algae blooms (HABs) casative Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides and Gymnodinium catenatum. The algicidal effect of MPB showed in the concentration of 31.3 $\mu{g}$/mL to 500 $\mu{g}$/mL against 4 HAB species and observed cell lysis or cell ecdysis by microscopy. MPB reacted more sensitive to C. marina and C. polykrikoides than A. tamarense and G. catenatum. The algicidal study of MPB against HABs will provides much insight into development of new algicidal substances.

Evaluation for Impacts of Nitrogen Source to Groundwater Quality in Livestock Farming Area

  • Lee, Gyeong-Mi;Park, Sunhwa;Kim, Ki-In;Jeon, Sang-Ho;Song, Dahee;Kim, Deok-hyun;Kim, Tae-Seung;Yun, Seong-Taek;Chung, Hyen Mi;Kim, Hyun-Koo
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.5
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    • pp.345-356
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    • 2017
  • We investigated 52 livestock farming complexes in Gyeong-Gi and Incheon provinces based on low, medium, and high livestock density and groundwater quality. The objective of this study was to evaluate a relationship between nitrate N concentration in groundwater and animal factors, such as livestock density and animal species. 2,200 groundwater samples for 3 years from 2012 to 2014 at Gyeong-Gi and Incheon provinces were collected and analyzed for pH, EC, DO, ORP, temperature, major anions and cations, such as $NO_3-N$, ${HCO_3}^-$, ${PO_4}^-$, ${SO_4}^{2-}$, $Cl^-$, $NH_4-N$, $K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, T-N, and TOC. Average concentration of total N for generated load density was $23,973g\;day^{-1}\;km^{-2}$ for cattle, $51,551g\;day^{-1}\;km^{-2}$ for pig, and $52,100g\;day^{-1}\;km^{-2}$ for poultry. For animal feeding species, average ratio for generated load over discharge load was 16.1% for cattle, 7.8% for pig, and 7.1% for poultry. Therefore, cattle feeding region is highly vulnerable for water pollution compared to pig and poultry feeding areas. The concentrations of chloride, nitrate, and total N in the groundwater samples were higher at high animal farming regions than other regions. The average concentration of nitrate, and chloride in groundwater samples was $5.0mg\;L^{-1}$, $16.6mg\;L^{-1}$ for low livestock density, $6.9mg\;L^{-1}$, $17.7mg\;L^{-1}$ for medium livestock density and $7.6mg\;L^{-1}$, $22.7mg\;L^{-1}$ for high livestock density and total nitrogen (T-N) was $7.7mg\;L^{-1}$ for low livestock density, $9.4mg\;L^{-1}$ for medium livestock density, $10.7mg\;L^{-1}$ for high livestock density. In conclusion, based on this research, for managing groundwater quality near livestock farming regions, $Ca-(Cl+NO_3)$ group from the Piper diagram is more efficient than using 19 factors for water quality standard.

Plant Regeneration from Mature Seed-Derived Callus in Bermudagrass (Cynodon dactylon) (난지형 목초 버뮤다그라스의 종자유래 캘러스로부터 식물체 재분화)

  • Lee, Ki-Won;Park, Hyung-Soo;Choi, Gi-Jun;Kim, Ki-Yong;Ji, Hee-Chung;Kim, Kyung-Hee;Lee, Byung-Hyun;Lee, Sang-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.3
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    • pp.223-228
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    • 2011
  • The present study was conducted to determine the optimum in vitro culture condition for callus induction and plant regeneration from mature seeds of bermudagrass (Cynodon dactylon cv. Common). It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/L 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (39.2%). The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.5 mg/L 2,4-D, 2 mg/L BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (57.7%). The frequency of callus induction and plant regeneration were the highest on sucrose, followed by maltose. The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium. Regenerated plants were morphologically uniform with normal growth pattern.

A Study on the Total Mercury (Hg) Monitoring and Methylmercury (MeHg) Analysis method and Exposure Assessment of Methylmercury (MeHg) in Marine Products (수산물 중 총수은 모니터링 및 메틸수은 분석법 고찰)

  • Kwak, Shin-Hye;Kim, Ki-Cheol;Kim, Kyung-A;Kang, Suk-Ho;Kwon, Hye-Jung;Cho, Yun-Sik;Kang, Kyung-Ja;Lee, Pil-Suk;Cho, Wook-Hyun;Moh, Ara;Park, Yong-Bae;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.33 no.3
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    • pp.168-175
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    • 2018
  • The use of microwave-assisted extraction and an acid-base clean-up process to determine the amount of methylmercury (MeHg) in marine products was suggested in order to improve the complicated sample preparation process. The optimal conditions for microwave-assisted extraction was developed by using a 10% NaCl solution as an extraction solution, setting the extraction temperature at $50^{\circ}C$, and holding for 15 minutes to extract the MeHg in marine products. A NaOH solution was selected as a clean-up substitute instead of L-cysteine solution. Overall, 670 samples of marine products were analyzed for total mercury (Hg). Detection levels were in the range of $0.0006{\sim}0.3801{\mu}g/kg$. MeHg was analyzed and compared using the current food code and the proposed method for 49 samples which contained above 0.1 mg/kg of Hg. Detection ranges of methylmercury followed by the Korea Food Code and the proposed method were $75.25(ND{\sim}516.93){\mu}g/kg$ and $142.07(100.14{\sim}244.55){\mu}g/kg$, respectively. The total analytical time of proposed method was reduced by more than 25% compared with the current food code method.

Bioaccumulation of Pb and Cd in Blue Mussel (Mytilus edulis) and Oliver Flounder (Paralichthys olivaceus) Exposed to Rearing Media (노출실험을 통한 진주담치(Mytilus edulis) 넙치(Paralichthys olivaceus)의 Pb 및 Cd 축적에 관한 연구)

  • Cho, Yeong-Gil;Kim, Gi-Beum
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.1
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    • pp.21-28
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    • 2007
  • The bioaccumulation of Pb and Cd dissolved in seawater was assessed measuring the concentrations recorded within blue mussel (Mytilus edulis) and oliver flounder (Paralichthys olivaceus) after two weeks exposure period. The Pb and Cd concentration within the whole body of two testing organisms increased according to the exposure concentrations, and the such tendency was clear specially from the mussel. Maximum metal concentration reached $5,260({\pm}70)\;{\mu}g/g$ for Pb reared under 5.0 mg/L Pb, $1,040({\pm}40)\;{\mu}g/g$ for Cd reared under 1.0 mg/L Cd in the mussel, and indicated that the bioaccumulation of Pb and Cd was directly related to the rearing medium concentrations. Bioconcentration factors (BCF) reached very high values for Pb (maximum value: $12,100{\pm}1,400$) in the mussel reared under lowest Pb concentration (0.01 mg/L). The BCF value for Cd in the mussel were also far higher at exposure to low Cd concentration than high Cd concentration. At higher external concentrations, the BCF for Cd and Pb declined. This demonstrated the ability of two testing organisms to rapidly uptake heavy metals particularly when exposed to low external concentration. The mean Pb concentration was slightly higher in the gill of mussel than in the digestive gland, while Cd showed a higher level in the digestive gland than in the gill.

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Some Trace Metals and their Ratios in Aloe (Aloe vera L.), Cucumber (Cucumis sativus L. var. tuberculatus Gabaj.) and Sponge-gourd (Luffa cylindrica L.) in Korea

  • Lee, Chang-Jun;Park, Jung-Sang;Jang, Gi-Chul;Kim, Sang-Deog A.
    • Korean Journal of Plant Resources
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    • v.22 no.6
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    • pp.540-545
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    • 2009
  • Aloe (Aloe vera L.), cucumber (Cucumis sativus L. var. tuberculatus Gabaj.) and sponge.gourd (Luffa cylindrica L.) are well utilized in Asian countries as traditional medicines and cosmetics, or foods. And we carried out an experiment to see if there are some specific ratios among trace metals on the plants. Though the metal concentrations varied significantly depending on the sampling periods, aloe gel is high in iron (Fe) and calcium (Ca). Cucumber fruit juice is also high in iron (Fe) and potassium (K). Sponge.gourd sap contains a high amount of zinc (Zn) and calcium (Ca). The uses of the previous 3 plant parts are considered to have some relations to their different ratios and of their trace metals.

Antibiotic Resistance and Genetic Diversity of Listeria monocytogenes Isolated from Chicken Carcasses in Korea

  • Jang Sung-Sik;Choo Eui-Young;Han Ki-Seon;Miyamoto Takahisa;Heu Sung-Gi;Ryu Sang-Ryeol
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1276-1284
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    • 2006
  • Listeria monocytogenes is a well-known high-risk foodborne pathogen that grows at refrigeration temperature and is responsible for outbreaks of listeriosis. We report here the incidence of L. monocytogenes in fresh chicken carcasses and present genetic diversity of L. monocytogenes isolates. In this study, 25 g of chicken carcasses from markets in Korea were examined according to the FDA method, and presumptive isolates were confirmed by multiplex PCR assay. L. monocytogenes isolates were analyzed by Pulsed-Field Gel Electrophoresis using restriction enzymes, ApaI and AscI, to obtain strain-specific DNA fragments profiles. Antimicrobial resistance of L. monocytogenes strains against generally used antibiotics (Penicillin G, Kanamycin, Tetracycline, Vancomycin, Cephalothin, Rifampicin, Erythromycin, Ampicillin, Gentamicin, Streptomycin, and Chloramphenicol) were analyzed by NCCLS protocols to examine the presence of antimicrobial resistance in natural L. monocytogenes. Of a total 274 chicken samples, 81 samples (29.6%) were positive for L. monocytogenes. Listeria innocua (50.1%), Listeria welshimeri (6.9%), and Listeria grayi (11.3%) were also detected. PFGE analysis, using restriction enzymes ApaI and AscI, showed 27 pulsotypes of L. monocytogenes. Antimicrobial resistance analysis confirmed the existence of antimicrobial resistance for penicillin G and tetracycline in isolated L. monocytogenes strains.