Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

The Algicidal Effect of Antimicrobial Peptide, Mastoparan B

항균성 펩타이드인 mastoparan B의 살조효과

  • Published : 20031200
Download PDF
⟨ Previous Next ⟩

Abstract

Mastoparan B (MPB), an antimicrobial cationic peptide isolated from the venom of the hornet Vespa basalis, is a basic amphipathic α-helical peptide composed of fourteen amino acid residues. In this study, we have investigated the algicidal effect of MPB against harmful algae blooms (HABs) casative Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides and Gymnodinium catenatum. The algicidal effect of MPB showed in the concentration of 31.3 $\mu{g}$/mL to 500 $\mu{g}$/mL against 4 HAB species and observed cell lysis or cell ecdysis by microscopy. MPB reacted more sensitive to C. marina and C. polykrikoides than A. tamarense and G. catenatum. The algicidal study of MPB against HABs will provides much insight into development of new algicidal substances.

Mastoparan B (MPB)는 벌독으로부터 정제된 양친매성 $\alpha$-helical 구조를 취하면서 14개의 아미노산 잔기로 구성된 염기성 항균성 펩타이드로서 여러가지 생물막과 작용한다. 본 연구에서는 우리 나라 연안의 적조 (HABs, harmful algal blooms)를 일으키는 4종의 적조생물 (Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides 및 Gymnodinium catenatum)에 대한 MPB의 살조효과를 조사하였다. MPB의 4종의 적조생물에 대한 살조효과는 31.3 $\mu{g}$/mL부터 500 $\mu{g}$/mL에서 세포의 lysis 또는 ecdysis와 같은 형태로 현미경으로 관찰할 수 있었다. 또한 MPB는 C. marina 및 C. polykrikorides에 대해서 A. tamarense와 G. catenatum보다 더욱 강한 살조효과를 나타내었다. 이러한 MPB의 HABs에 대한 살조효과연구는 새로운 살조물질을 개발하기 위한 자료가 될 것으로 생각된다.

Keywords

References

  1. Choi, H. G., Kim, D. J., Lee, W. C., Yun, S. J., Kim, H. G. and Lee, H. J. : Removal efficiency of Cochlodinium polykrikoides, by yellow loess. J. Korean Fish. Soc., 31 : 109-113, 1998
  2. Goutx, M., Momziko, A., Striby, L., Andersen, V., Marty, J. C. and Vescovali, I. : High-frequency fluxes of labile compounds in the central ligurian Sea, northwestern Mediterranean. Dee-Sea Research. 47 : 533-556, 2000 https://doi.org/10.1016/S0967-0637(99)00101-6
  3. Fukami, K., Yuzawa, A., Nishijima, T. and Hata, Y. : Isolation and properities of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi. 58(6) : 1073-1077, 1992 https://doi.org/10.2331/suisan.58.1073
  4. Hallegraeff, G. M. : A review of harmful algal blooms and their apparent global increase. Phycologia, 32 : 79-99, 1993 https://doi.org/10.2216/i0031-8884-32-2-79.1
  5. Han, M. S., Jeon, J. K. and Yoon, Y. H. : Distribution and toxin profiles of Alexandrium tamarens (Lebour) Balech (diouglagellate) in the southeastern coastal waters, Korea. Korean J. Physic., 8(1) : 7-13, 1993
  6. Ho, C-.L. and Hwang, L-L. : Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis. Biochem. J. 274 : 453-456, 1991 https://doi.org/10.1042/bj2740453
  7. Imai, I., Ishda, Y. and Hata, Y. : Killing of marine phytoplankton by a gliding bacterium Cytophaga sp., isolated from the coastal sea of Japan. Mar. Biol., 116 : 527-532, 1993
  8. Ishio, S., Mangidan, R. E., Kuwahara, M. and Nakagawa, H. : Abacterium hostile to flagellates: identification of species and characters. In Red tide-biology, Environmental science and toxicology, Okaichi, D., M. Anderson and T. Nemoto, ends. Elsevier Sci., N. Y., : 205-208, 1989
  9. Koji, K., Yuki, M. and Naganuma, J. : Removal of biofouling and red tide alage by Triosyn. Abstract of 2nd Meeting for Japan Marine Biotechnology. : 89, 1998
  10. Na, G. H., Choi, W. J., and Chun, Y. Y. : A study on red tide control with loess suspenson. Kor. J. Aquacult. 9 : 239-245, 1996
  11. Park, N. G., Seo, J-K., Ku, H-J., Kim, S-H., Lee, S., Sugihara. G., Kim, K-H., Park, J-S. and Kang, S-W. : Interaction of mastoparan B and its Ala-substituted analogs with phospholipid bilayers. Bull. Korean Chem. Soc., 18(9) : 933-938, 1997
  12. Park, N. G., Kim, C. H., Chung, J. K., Huh, M-D., Park, J-S. and Kang, S-W. : Morphological changes of biomembranes by amphiphilic basic peptides mastoparan B and 43. J. Microbiol., 179-183, 1998
  13. Park, J. S. : Red tide occurrence and countermeasure in Korea. In Recent approaches on red tides, J. S. Park and H. G. Kim, Eds. Department of Oceanography and Marine Resources, National Fisheries Research & Deveolpment Agency, Korea, : pp. 1-24. 1991
  14. Park. Y. T., Park, J. B., Chung, S. Y., Song, B. C., Lim, W. A., Kim, C. H., and Lee, W. J. : Isolation of marine bacteria killing red tide microalgae-I. Isolation and algicidal properties of Micrococcus sp. Lg-I possessing killing activity for harmful dinoflagellate, Cochlodinium polykrikoides. J. Korean Fish. Soc., 31(5) : 767-773, 1998
  15. Ryu, H. Y., Shim, J. H., Bang, J. D. and Lee, C. : Experimental chemical treatment for the control of dinoflagellate, Cochlodinium polykrikoides in the land-based culture of olive flounder Paralichthys olivaceus. Kor. J. Aquacult. 11 : 285-294, 1998
  16. Sakata, T., Fujita, Y. and Yasumoto, H. : Plaque formation by algicidal Saprospira sp. on a lawn of Chaetoceros ceratosporum. Nippon Suisan Gakkaishi. 57 : 1147-1152, 1991 https://doi.org/10.2331/suisan.57.1147
  17. Seo, P-S., Lee, S-J., Kim, Y., Lee, J-H., Kim, H-G. and Lee, J-D. : Axenic culture production and growth of a Dinoflagellate, Cochlodinium polykrikoides. J. Korean Fish. Soc. 31(1) : 71-76, 1998
  18. Steidinger, K. A. : A re-evalution of toxic dinoflagellate biology and ecology. Prog. Phycol. Res. 2 : 147-188, 1983
  19. Yoshinaga, I., Kim, M. C., Katanozaka, N., Imai, I., Uchida, A. and Ishisa, Y. : Population structure of algicidal marine bacteria targeting Heterosigma akashiwo (Raphidophyceae) through restriction fragment length polymorphin analysis of the bacterial 16S ribosomal RNA genes, during H. akashiwo red tide. Mar. Ecol. Prog. Ser., 170 : 33-44, 1998 https://doi.org/10.3354/meps170033
  20. 김학균, 이삼근, 안경호, 윤성화 : 한국연안의 유독성 Cochlodinium 적조 발생과 변천, 제 2회 연구발표 및 귀국보고, 국립수산진흥원 : pp. 23-24. 1996