• Journal of Korean Society of Water and Wastewater
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• v.26 no.6
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• pp.767-777
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• 2012

Annular Biofilm Reactor (ABR) equipped with coupons of three different pipe materials (STS 304, PVC, PE) was used to generate drinking water biofilm samples. The level of assimilable organic carbon (AOC) during the sample generation period was $37.3{\mu}g/L$, and this level did not seem to be low enough to limit the formation of biofilm in this study. Terminal-restriction fragment length polymorphism (T-RFLP) analyses determined T-RF profile as early as 3 h of exposure on PVC coupons. Average surface roughness ($R_a$) measured by atomic force microscopic analyses was 125.7 nm for PVC, and this value was higher than for STS (71.6 nm) and PE (74.0 nm). However, biofilm formation was faster on STS (6 h) than on PE (12 h), which indicated that surface roughness might not be the only factor that controlled the initiation of biofilm development. Upon detection of the T-RF peaks, richness (S) and diversity indices such as Shannon (H) and Simpson (1/D) demonstrated a rather slow increase until 48 h followed by rapid increase regardless of the pipe materials. Differences of microbial community structures among the biofilm samples were determined based on the cluster analysis using Jaccard coefficients (Sj). Biofilm communities could be divided into two distinct groups according to the exposure time regardless of the pipe materials. First group contained a young (< 48 h) biofilm samples (10 out of 11) but second group contained a mature (${\geq}$ 48 h) samples (11 out of 14). Results suggested that, due to the complexity of biofilm, the targeting of the first group of cluster was crucial for optimizing the management of drinking water distribution systems and controlling microbial growth.

• Journal of Aquaculture
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• v.19 no.4
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• pp.299-304
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• 2006

This study was conducted to investigate the effects of protein and lipid levels of extruded pellet on growth and body composition of juvenile flounder. Six extruded dry pellets were formulated to contain two protein levels (50 and 55%) and three lipid levels (8, 12 and 15%). Triplicate groups of fish (initial mean weight 21 g) were hand-fed to apparent satiation two times a day for 9 weeks. Survival, weight gain and daily feed intake were not significantly affected by dietary protein and lipid levels. Feed efficiency and protein efficiency ratio were significantly (P<0.001) affected by dietary lipid level, and tended to increase as dietary lipid level increased. Feed efficiency ratio of fish fed the 55% protein diet with 15% lipid was not significantly different from that of fish fed the 50% protein diet with 15% lipid, but significantly (P<0.05) higher than that of fish fed the other diets. Protein efficiency ratio of fish fed the 55% protein diet with 15% lipid was significantly (P<0.05) higher than that of fish fed the 50% protein diet with 8% lipid and 55% protein diets with $8{\sim}12%$ lipids. The contents of moisture, crude protein and lipid in the liver was significantly (P<0.01) affected by dietary lipid level. The results of this study indicate that an increase in lipid level at 50-55% protein in extruded pellet can improve feed efficiency for juvenile flounder.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1066-1074
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• 2015

In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five YSNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (${\pi}=5.6{\times}10^{-4}$) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (${\pi}=0.00000$) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.1
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• pp.51-69
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• 2000

This dissertation was to research how some metal level within SipJeonDaeBo - Decoction, one of oriental prescriptions, influence Sprague-Dawley animals. 1. Under the experiment with drinking waters there was no metal ${\sim}0.65\;mg/L$ detected. A metal with feed found 0.001-376.983mg/kg. 2. In the mice's kidney, brain, bones used experiment, As searched 0.474 mg/kg, 0.486 mg/kg, 0.314 mg/kg 0.834 mg/kg respectively ; Cd 0.060 mg/kg, 0.045 mg/kg, 0.030 mg/kg, 0.353 mg/kg, ; Co 0.105 mg/kg, 0.063 mg/kg, 0.030 mg/kg, 0.399 mg/kg, ; Cr 0.292 mg/kg, 0.304 mg/kg, 0.234 mg/kg, 0.962 mg/kg, ; Cu 4.201 mg/kg, 3.759 mg/kg, 1.923 mg/kg, 0.484 mg/kg, ; Fe 57.535 mg/kg, 150.571 mg/kg, 17.178 mg/kg, 281.506 mg/kg, ; no Hg, Mn 0.612 mg/kg, 2.968 mg/kg, 0.528 mg/kg, 4.205 mg/kg, ; Ni 0.094 mg/kg, 0.072 mg/kg, 0.078 mg/kg, 27.714 mg/kg, ; Pb 0.269 mg/kg, 0.293 mg/kg, 0.283 mg/kg, 43.142 mg/kg ; Zn 4.149 mg/kg, 21.861 mg/kg, 8.088 mg/kg, 226.283 mg/kg respectively. 3. In level of hazardous metal within idney control group searched 0.194 {\pm}\; 0.052 mg/kg, experimental I g개up $0.189{\pm}0.036\;mg/kg$, experimental I group $0.264 {\pm}{\pm}\;0.179\;mg/kg$. In level of non hazardous metal control group searched $15.917{\pm}5.575\;mg/kg$, experiment I group $17.064{\pm}2.246\;mg/kg$, experiment II group $16.892{\pm}3.586\;mg/kg$. Besides in total level of metal control g.cup detected $6.484{\pm}2.258\;mg/kg$, experiment I group $6.940{\pm}0.914\;mg/kg$, experiment II group $6.915{\pm} 1.508\;mg/kg$ There all was no statistical significance. 4. In level of hazardous metal within the liver control group searched $0.187{\pm}0.048\;mg/kg$, experiment I g개up $0.168[\pm}0.079\;mg/kg$, experiment II group $0.277{\pm}0.159\;mg/kg$. In level of non hazardous heavy metal control group detected $44.925{\pm}18.468\;mg/kg$, experiment I group $39.917{\pm}12.772\;mg/kg$, experiment II group $49.525{\pm}33.484\;mg/kg$. Besides in total concentration control group searched $18.082{\pm}7.395\;mg/kg$, experiment I group $16.068{\pm}5.128\;mg/kg$, experiment II group $19.977{\pm}13.443\;mg/kg$. There was no statistical significance but hazardous metal gets more level in the experilnent group than in the control group. 5. In level of hazardous metal within brain control group searched $0.145{\pm}0.056\;mg/kg$, experiment I group $$0.167{\pm}0.030\;mg/kg, erperiment II group $0.172{\pm}0.123\;mg/kg$. In level of non hazardous heavy metal control group detected $6.488{\pm}0.965\;mg/kg$, experiment I group $7.290{\pm}0.588\;mg/kg$, experiment II group $7.010{\pm}1.627\;mg/kg$. Besides in total concentration control group searched $2.683{\pm}7.395\;mg/kg$, experiment I group $3.017{\pm}0.238\;mg/kg$, experiment II group $2.908 {\pm} 0.711\;mg/kg$. Therefore there was no statistical significance. 6. In level of hazardous metal within bone control group searched $8.172{\pm}5.195 \;mg/kg$, experiment I group $9.128{\pm}4.143\;mg/kg$, experiment II group $9.401{\pm}6.924\;mg/kg$. There is statistical significance(p<0.05). In level of non hazardous metal control group detected $94.065{\pm}36.035\;mg/kg$, experiment I group $147.563 {\pm}79.939\;mg/kg$, experiment II group $142.730{\pm}77.374\;mg/kg$. Besides in total level control group searched $48.530{\pm}16.523\;mg/kg$, experiment I group $64.502{\pm}31.078\;mg/kg$, experiment II group $62.733 {\pm}34.641\;mg/kg$. Therefore there was no statistical significance. 7 In the correlative research as to how each metal influences to ingestion Cd and Co searched 0.954 and Pb and Ni -0.0884 from kidney. Co and Cd was 0.995 and Zn and As -0.190 from liver. Co and Cd were 0.995 and Zn and Cu -0.393 from brain. Co and Cd were 0.998 and Zn and Mn -0.206 from bones

• Korean Journal of Medicinal Crop Science
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• v.18 no.5
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• pp.298-304
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• 2010

For the investigation of possibility as a useful functional material, different parts of Lythrum salicaria L. harvested at four growth stages were studied in the aspect of bleeding characteristics, chemical composition and in vitro activity. Weights (g/plant) of L. salicaria plant parts were high in order to stem > leaf > flower > root at the best growth time. Crude lipids (3.59~4.30%) and crude proteins (14.7~23.5%) of L. salicaria leaves were the highest among the other plant parts showed from 0.08~3.54%, and 4.0~21.9%, respectively. Free sugars (2.9~4.2%) and crude ash (11.9~14.8) of leaves also showed the highest value. Free radical scavenging activities of L. salicaria root on 2,2-diphenyl-1-picrylhydrazyl showed from $43.5\;{\mu}g/m{\ell}$ to $47.6\;{\mu}g/m{\ell}$ as $IC_{50}$ which were followed by those of flower, leaf, and stem. Root of L. salicaria tested at $100\;{\mu}g/m{\ell}$ also showed the most efficient inhibitory effect on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in murine macrophage RAW264.7 cells. Cell viability of the plant parts tested by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazoliumbromide (MTT) assay was high in order to flower, leaf, root, and stem. Total phenol content measured as tannic acid equivalent showed the highest value in flower. In conclusion, among the plant parts, especially leaf of L. salicaria, was rich in the chemical components, and showed efficient antioxidant/inhibitory activity on free radical and NO production, and was expected to be a functional material candidate.

• The Korean Journal of Nuclear Medicine
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• v.32 no.3
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• pp.298-304
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• 1998

Purpose: We evaluated the usefulness of Re-188 sulfur colloid for radiation synovectomy and therapy of intraperitoneal metastasis. Materials and Methods: We investigated the labeling efficiency of Re-188 sulfur colloid on various conditions. The stability of Re-188 sulfur colloid was observed at room temperature for 24 h and in human serum and synovial fluid for 72 h. The particle size distribution of Re-188 sulfur colloid was measured by filtering with various pore size filters. Animal experiment was performed in mice and rabbits. Results: The labeling efficiency of Re-188 sulfur colloid was $64.5{\pm}5.8%$ (n=5) at the conditions of sodium thiosulfate 40 mg, EDTA $Na_2.2H_2O$ 0.8 mg, $KReO_4$ 0.8 mg at pH 1. After purification, the radiochemical purity was higher than 99%. The stability of Re-188 sulfur colloid was high (>99%) at room temperature for 24 h and in human serum and synovial fluid for 72 h. The particle size distribution of Re-188 sulfur colloid was 0.3% ($<1{\mu}m$), 11.2% ($1{\sim}5{\mu}m$), 25.8% ($5{\sim}10{\mu}m$) and 52.8% ($>10{\mu}m$). In mice, 1 h postinjection of Re-188 sulfur colloid into tail vein, uptakes in lung, liver and muscle were $37.30{\pm}5.36$, $32.33{\pm}1.79$, $6.60{\pm}0.02%$ ID/organ respectively. After i.p. injection in mice, the uptakes of extraperitonial organs of Re-188 sulfur colloid at 1 and 24 h were $0.1{\pm}0.1$, $0.4{\pm}0.1%$ ID/organ, and the excretions through urine and feces (${\sim}70 h$) were low ($2.68{\pm}0.80$, $0.95{\pm}0.17%$). When Re-188 sulfur colloid was injected to synovial space of rabbit, the uptake in other organs except knee was very low. Conclusion: Re-188 sulfur colloid showed high labeling efficiency, stability and potency for clinical use.

• The Journal of Korean Academic Society of Nursing Education
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• v.6 no.2
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• pp.376-389
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• 2000

The Purpose of this study was to grasp the status of drug uses in college students, and to investigate the knowledge and attitude when they use it. We collected through questionnaires from Sep. 1 to Oct. 30 in 1999. The subjects were 490 college students in Seoul and northern of the Kyunggi-do. The data analysis was performed using SPSS (version 7.0) and ANOVA. The results of this study were as follows. 1. There was no correlation for all factors in the relationship between general characteristics of subjects and knowledge for drugs. On the contrary, in the relation of the general characteristics and attitude for drug of subjects, some factors are statistically significant e.g., department (F=3.049, p<.010), satisfaction for school life (F=6.167,p<.002), body shape(F=8.092, p<.000), and the relationship with ones parents (F=3.728, p<.005). 2. In the analysis of drug use status and knowledge, only in the factor of administration control was statistically significant(F=4.304, p<.014) and in the relation of attitude for drug uses, drug administration was statistically significant (F=4.521, p<.004). 3. In the mean scores for the drug knowledge analysis, the highest factor was 'A narcotic make deformed persons through poisoning of physical and mental' (M=4.14), the lowest factor was 'If catch the flu during the pregnancy, should be take drug as possible as quickly to reduce negative effect for fetal' (M=1.94). 4. In the analysis for drug attitude, the highest factor was 'A drug is alike a poison' (M=3.48), 'Should be keep the usage and dosage of drug' was the lowest (M=1.48). 5. From the investigation for status of drug use, it was revealed that the most subjects (73.6%) were purchase drugs after explain their symptoms to pharmacist. And they take drugs only when they felt painful in 43.1%. The most students (70.4%) were experienced control of drug administration. It was inquired that subjects were mainly obtained information about drugs from pharmacist and television (or radio) advertisement, 33.5% and 33.1%, respectively. In the examination for existence of long-term administrators in their family, 'none' and 'only parents' are 49.6% and 37.3%, respectively. When their parents have illness, the persons go to drugstore and hospital for heath-care, 47.8% and 44.3%, respectively. On the basis of results of this study, we suggest as follows. 1. This study was analyzed data from questionnaires for college students in a part of local areas, so we suggest that the next research should be perform for national-wide students as subjects to generalize the results. 2. It is need more intensive research methodologies such as interview and observation. 3. Additional research is required for knowledge and behaviors of drug uses that will how impact on ones health behavior.

• Journal of the East Asian Society of Dietary Life
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• v.20 no.6
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• pp.853-862
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• 2010

This study was conducted as a nationwide project in order to obtain data on the content and intake of sodium in school meals. For this purpose, we selected two elementary and two middle schools in Daejeon as well as four elementary schools in the Chungcheong area. We measured the sodium and potassium contents of 842 dishes as well as their intake levels over 154 days. Sodium content was analyzed using an AAS (Atomic Absorption Spectrometer). The average meal intakes of second and fifth grade elementary school students were 244 g/meal and 304 g/meal, respectively. The meal intake of middle school students was 401 g/meal. Boys tended to eat slightly more than did girls, and students tended to eat more in the spring than in the summer. The average sodium intake per meal was 642 mg (570 mg by elementary school students, 1068 mg by middle school students), which was 32.1% of the UL (Tolerable Upper Intake Level, 2,000 mg per day) according to the KDRIs (Dietary Reference Intakes for Koreans). The sodium intake per meal was not significantly differ between boys and girls or between spring and summer (p>0.05). This study provides fundamental data that can be used to establishment a nutrition policy concerning the adequate content of sodium in school meals.

• Tuberculosis and Respiratory Diseases
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• v.47 no.3
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• pp.304-310
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• 1999

Background: IFN-$\gamma$ plays an important role in host response to intracellular organisms such as mycobacterium. Human infection with mycobacterium leads to a wide variety of outcomes, ranging from asymptomatic infection to widespread and rapidly fatal disease. Recent reports suggest that alteration of the function of IFN-$\gamma$ caused by a defective IFN-$\gamma$ receptor gene can explain different host response to mycobacterium. In this study, we investigated the role of IFN-$\gamma$ in the development of chronic refractory tuberculosis. Methods: The LPS-induced TNF-$\alpha$ production with or without IFN-$\gamma$ priming was compared by using monocytes taken from recently diagnosed tuberculosis, chronic refractory tuberculosis patients and controls. And the IFN-$\gamma$ receptor was measured by indirect fluorescent antibody technique to know whether change in the priming effect of IFN-$\gamma$ is related to IFN-$\gamma$ receptor deficiency or not. Results: The ratio of TNF-$\alpha$ produced in response to stimulation with INF-$\gamma$ and LPS to LPS alone was $13.5{\pm}7.6$ in controls, $10.8{\pm}6.4$ in recently diagnosed tuberculosis patients and $6.7{\pm}3.9$ in chronic refractory tuberculosis patients. The priming effect of IFN-$\gamma$ significantly decreased in chronic refractory tuberculosis patients compared with that in controls(p=0.002). However, IFN-$\gamma$ receptor deficiency was detected in one of chronic refractory tuberculosis patients. Conclusion: The decrease of the priming effect of IFN-$\gamma$ may play an important role in the development of chronic refractory tuberculosis, and in some patients, this may be related to the IFN-$\gamma$ receptor deficiency.

• Journal of the Korean Society for Nondestructive Testing
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• v.18 no.6
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• pp.455-463
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• 1998

RCCA(rod cluster control assembly) End-Tip suffers from neutron irradiation and constant vibration due to high-speed internal flow of primary coolant during plant operation. Such operating conditions cause the RCCA end-tip crackings around tile circumferential weldment of the end-tip, and in some cases, the defective end-tips were completly broken loose. However, no reliable inspection techniques for end-tip crackings were developed in the past, although some techniques exist for inspecting RCCA control rod wears. Therefore, NDE group at KEPRI has developed an ECT technique for the detection and the sizing of the end-tip crackings. The technique uses a specially designed surface-riding probe that can detect size of circumferential crackings with an accuracy of ${\pm}5.31%$ RMS error. This paper describes the ECT instrumentation including the ECT probes, calibration bars, as well as technical approaches.