• 생약학회지
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• 제35권4호통권139호
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• pp.380-383
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• 2004

The purpose of this study was to evaluate mushroom Tyrosinase inhibitory activity of Cuscuta japonica Choisy and C. australis R.Be. The experimental materials were expressed juice from their stems and flowers, both water and ethanol extracts, their seeds, and two kinds of commercially available cosmetic packing Wontosa and Bupjetosa (made from seeds of C. japonica). The 50% inhibitory concentration $(IC_{50})$ of C. Japonica juice was 5.4 mg/ml. However, C. australis juice showed negligible mushroom tyrosinase inhibitory activity. The $IC_{50}$ of water extracted C. japonica seed was $54.0\;{\mu}g/ml$, water extracted product of Wontosa $50.0\;{\mu}g/ml$ and Bupjetosa $40\;{\mu}g/ml$. The $IC_{50}$ of ethanol extracted C. japonica seed was $10\;{\mu}g/ml$, Wontosa $10\;{\mu}g/ml$ and Bupjetosa $20\;{\mu}g/ml$.

• Natural Product Sciences
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• 제21권1호
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• pp.20-24
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• 2015

Cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside (C3R) were isolated by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (1 : 3 : 1 : 5 : 0.01, v/v) to give pure C3G (34.1 mg) and C3R (14.3 mg) from 1.5 g crude mulberry fruit extract. Using the pure C3G and C3R, a reliable high-performance liquid chromatography (HPLC) method was developed and validated to determine the C3G and C3R contents in mulberry fruit. C3G and C3R were separated simultaneously using an Eclipse XDB-C18 column ($4.6{\times}250mm$ I.D., $5{\mu}m$) coupled with a photodiode array detector (PDA). The gradient elution of the mobile phase consisting of acetonitrile (0.5% formic acid) and water (0.5% formic acid) was applied (1.0 mL/min), and the detection wavelength was 520 nm. The calibration curves of C3G and C3R showed good linearity (both with $r^2=0.9996$) in the concentration range $15.625-500{\mu}g/mL$, and the relative standard deviations (RSD%) of intra- and inter-day variability were in the ranges 2.1 - 8.2% and 4.1 - 17.1%, respectively. The accuracies were ranged 96.5 - 102.6% for C3G and C3R, respectively. The developed HPLC method was used to determine the contents of C3G and C3R in newly harvested mulberry from eight different provinces of Korea.

• 한국컴퓨터정보학회논문지
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• 제16권7호
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• pp.85-93
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• 2011

본 논문은 지금까지 NP-완전인 난제로 알려진 정점 색칠 문제를 선형시간 복잡도로 해결한 알고리즘을 제안하였다. 제안된 알고리즘은 그래프 G=(V,E)의 최소 채색수 ${\chi}(G)$=k를 결정하기 위해 사전에 k값을 알지 못한다는 가정에 기반하고 있다. 단지 주어진 그래프를 독립집합 $\overline{C}$와 정점 피복 집합 C로 정확히 양분하여 $\overline{C}$에 색을 배정하는 방법을 적용하였다. 독립집합 $\overline{C}$의 원소는 ${\delta}(G)$인 정점 ${\upsilon}$가, C의 원소는 정점 ${\upsilon}$의 인접 정점들 u가배정된다. 축소된 그래프 C는 다시 $\overline{C}$와 C로 양분되며, 이 과정을 C의 간선이 없을 때까지 수행한다. 26개의 다양한 그래프를 대상으로 제안된 알고리즘을 적용한 결과 정점 ${\upsilon}$를 선택하는 횟수는 정점의 수 n보다 작은 값을 나타내었으며, ${\chi}(G)$=k를 찾는데 성공하였다.

• 한국환경과학회지
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• 제10권4호
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• pp.293-298
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• 2001

The toxicity values heavy metals were evaluated by immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange or water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3~1.4 and DO also increased. 2) The $E_iC_{50}$ values of immobilization to Daphnia magna in artificial fresh water were $0.030mg/{\ell}(Cu),\;0.054mg/{\ell}(Cd),\;0.12mg/{\ell};(Cr),\;0.74mg/{\ell}(Pb),\;3.4mg/ {\ell}(As)$ and the $NOE_iC$ values were $0.010mg/{\ell}(Cu),\;0.018mg/{\ell}(Cd),\;0.010mg/{\ell}(Cr),\;0.10mg/{\ell}(Pb),\;and\;$1.8mg/{\ell}(As)$. 3) The $E_rC_{50}$ values of reproductive impairment to Daphnia magna were $13.8\mu\textrm{g}/{\ell}(Cu),\;2.9\mu\textrm{g}/{\ell}(Cd),\;15.5\mu\textrm{g}/{\ell}(Cr),\;61.7\mu\textrm{g}/{\ell}(Pb),\;759\mu\textrm{g}/{\ell}(As)$, and $NOE_rC$ values were $0.95\mu\textrm{g}/{\ell}(Cu),\;$0.54\mu\textrm{g}/{\ell}(Cd),\;1.2\mu\textrm{g}/{\ell}(Cd),\;$7.4\mu\textrm{g}/{\ell}(Pb),\;110mu\textrm{g}/{\ell}(As)$. The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of marterials.

• Natural Product Sciences
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• 제22권4호
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• pp.282-286
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• 2016

Cyanidin-$3{\beta}$-D-glycoside (C3G), which is widely distributed in herbal medicines and functional foods, exhibits anti-inflammatory, anti-oxidant, and anti-scratching behavioral effects. Orally administered C3G is metabolized to protocatechuic acid (PA) by gut microbiota. Therefore, we compared the anti-colitic effect of C3G to that of PA in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Orally administered C3G and PA preventively and curatively ameliorated TNBS-induced colitis parameters, including macroscopic colitis score, colon shortening, and increase of myeloperoxidase activity. Treatment with C3G or PA also inhibited the expression of cyclooxygenase-2, inducible NO synthatase, $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ and the activation of $NF-{\kappa}B$ in the colon of mice with TNBS-induced colitis. Furthermore, these also inhibited lipopolysaccharide-induced $NF-{\kappa}B$ activation and $TNF-{\alpha}$ expression in peritoneal macrophages. The anti-colitic effect of PA was more effective than C3G. Orally administered PA more potently attenuate colitis than C3G by inhibiting $NF-{\kappa}B$ activation and the anti-colitic efficacy of C3G may be dependent on the biotransformation of C3G to PA by gut microbiota.

• Journal of Information Technology Applications and Management
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• 제16권4호
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• pp.205-222
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• 2009

Considering time-lag in the performance evaluation of information system (IS) investment is important because its effect reveals after certain period of time passed. Particularly it is more in the systems of e-government informatization projects which the amount of investment and the scale of business are huge. Many methods to solve this issue have been proposed such as system dynamics methods, simulations, structural equations etc. However, it is still difficult and unsolved problem because collecting practical data for time-lag analysis is very hard. In this paper, we analyze IS time-lag effect through factor analysis using the accumulated practical operational DB data. For the performance evaluation of the G4C system, the representative e-government web portal, we selected eleven factors reflecting time passing in G4C DB data. With these factors this paper conduct time-lag analysis in four view points. First, we conducted 'Stabilizing of G4C system' and got a result that IS is needed about three years for the stabilization. Second, we conducted 'Utilization of G4C system' and got a result that the utilization reaches appropriate level after in three years later after the introduction of G4C system. Third, we conducted 'Cost reduction effect' and got a result that cost reduction is stable in the third year after the introduction of G4C system. Lastly, we conducted 'System maturity effect' and got a result that the system reaches to the quality level that users expect after third to fourth years. According to the results of this research, we found that performance of IS improv continuously not immediately, and it needs three or four years of time-lag.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1689-1695
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• 2008

Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p<0.05). Analysis of the combined genotype effect in both SNPs showed that animals with the AC SSCP genotype had higher BF value than animals with BB or AA SSCP genotypes (p<0.05). These results suggest that SNP of the leptin promoter region may be useful markers for selection of economic traits in Korean cattle.

• Korean Journal of Mathematics
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• 제29권1호
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• pp.41-55
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• 2021

Woven frames are motivated from distributed signal processing with potential applications in wireless sensor networks. g-frames provide more choices on analyzing functions from the frame expansion coefficients. The objective of this paper is to introduce woven g-frames in Hilbert C∗-modules, and to develop its fundamental properties. In this investigation, we establish sufficient conditions under which two g-frames possess the weaving properties. We also investigate the sufficient conditions under which a family of g-frames possess weaving properties.

• 호남수학학술지
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• 제10권1호
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• pp.23-30
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• 1988

Let C[C, D] and $S^{*}[C,\;D]$ denote the classes of functions g, g(0)=1-g'(0)0=0, analytic in the unit disc E such that $\frac{(zg{\prime}(z)){\prime}}{g{\prime}(z)}$ and $\frac{zg{\prime}(z)}{g(z)}$ are subordinate to $\frac{1+Cz}{1+Dz{\prime}}$ $z{\in}E$, respectively. In this paper, the classes K[A,B;C,D] and $C^{*}[A,B;C,D]$, $-1{\leq}B<A{\leq}1$; $-1{\leq}D<C{\leq}1$, are defined. The functions in these classes are close-to-convex. Using the properties of convolution operators, we deal with some problems for our classes.

• 약학회지
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• 제57권5호
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• pp.309-315
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• 2013

Cyanidin-3-glucoside (C3G), an anthocyanin, is one of the most widespread dietary flavonoids. We investigated the effects of C3G in PMA/ionomycin (PI)-induced RBL-2H3 cells. C3G inhibited the production of IL-4 and IL-13 and also decreased the level of mRNA in a dose-dependent manner. Furthermore, western blot analysis implied that C3G down-regulated the protein level of c-Jun, NF-ATc1 and NF-${\kappa}B$ but not c-Fos. Taken together, we suggest that C3G may have suppressive effects on Th2 cytokines and will be studied further to develop as functional foods that help alleviate allergy symptoms.