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Ultrastructure of the flagellar apparatus in Rhinomonas reticulata var. atrorosea (Cryptophyceae, Cryptophyta)

  • Nam, Seung Won;Go, Donghee;Son, Misun;Shin, Woongghi
    • ALGAE
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    • v.28 no.4
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    • pp.331-341
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    • 2013
  • Rhinomonas reticulata var. atrorosea G. Novarino is a photosynthetic marine flagellate that is known to have typical characteristics of cryptomonads. We examined the flagellar apparatus of R. reticulata var. atrorosea by transmission electron microscopy. The major components of the flagellar apparatus of R. reticulata var. atrorosea consisted of four types of microtubular roots (1r, 2r, 3r, and mr), a non-keeled rhizostyle (Rhs), mitochondrion-associated lamella (ML), two connections between basal bodies, a striated fibrous root (SR) and a striated fiber-associated microtubular root (SRm). Four types of microtubular roots originated near the ventral basal body and extended toward the left side of the basal bodies. The non-keeled Rhs originated at the Rhs-associated striated fiber, which was located between two basal bodies and extended into the middle of the cell. The ML was a plate-like fibrous structure associated with mitochondria and originating from a Rhs-associated fiber. It split into two parts and extended toward the dorsal-posterior of the cell to a mitochondrion. The SR and SRm extended parallel to the anterior lobe of the cell. The overall configuration of the flagellar apparatus in R. reticulata var. atrorosea was similar to the previously reported descriptions of those of Cryptomonas paramecium, C. pyrenoidifera, C. ovata, Hanusia phi, Guillardia theta, and Proteomonas sulcata. However, the flagellar apparatus system of R. reticulata var. atrorosea was more complex than those of other cryptomonad species due to the presence of an additional microtubular root and other distinctive features, such as a rhizostyle-associated striated fiber and large ML.

Experimental & Numerical Result of the filling of Micro Structures in Injection Molding (미세 구조물의 충전에 관한 실험 및 수치해석)

  • Lee J.G.;Lee B.K;Kwon T.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.10a
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    • pp.111-114
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    • 2005
  • Experimental and numerical studies were carried out in order to investigate the processability and the transcriptability of the injection molding of micro structures. For this purpose, we designed a mold insert having micro rib patterns on a relatively thick base part. Mold insert has a base of 2mm thickness, and has nine micro ribs on that base plate. Width and height of the rib are $300{\mu}m\;and\;1200{\mu}m$, respectively. We found a phenomenon similar to 'race tracking', due to 'hesitation' in the micro ribs. As the melt flows, it starts to cool down and melt front located in the ribs near the gate cannot penetrate further because the flow resistance is large in that almost frozen portion. When the base is totally filled, the melt front away from the gate is not frozen yet. Therefore, it flows back to the gate direction through the ribs. Consequently, transcriptability of the rib far from the gate is better. We also verified this phenomenon via numerical simulation. We further investigated the effects of processing conditions, such as flow rate, packing time, packing pressure, wall temperature and melt temperature, on the transcriptability. The most dominant factor that affects the flow pattern and the transcriptability of the micro rib is flow rate. High flow rate and high melt temperature enhance the transcriptability of micro rib structure. High packing time and high packing pressure result in insignificant dimensional variations of the rib. Numerical simulation also confirms that low flow rate causes a short shot of micro ribs and high wall temperature helps the filling of the micro ribs.

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Pressure Drop and Vibration Characteristics of the Capsule with the Modification of Bottom Structures (캡슐 하단부 구조변경에 따른 압력강하 및 진동특성)

  • Choi, M.H.;Choo, K.N.;Cho, M.S.;Lee, K.H.;Kim, B.G.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.11a
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    • pp.782-787
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    • 2005
  • The bottom structure of an instrumented capsule is a part which is joined at the receptacle of the flow tube in the reactor in-core. A geometrical change or the bottom structure has an effect on the pressure drop and the vibration of the capsule. The out-pile test to evaluate the structural Integrity of the material capsule called 04M-l7U was performed by using a single channel and a half core test loop. From the pressure drop test, the optimized diameter of the cone shape's bottom structure which satisfies HANARO's flow requirement (19 6 kg/s) is 71 mm. The maximum displacement of the capsule measured at the half core test loop is lower than 1.0 mm. From the analysis results, it is found that the test hole will not be interfered with near the flow tubes because its displacement due to the cooling water is very small at 0.072 mm. The fundamental frequency of the capsule under water is 9.64 Hz. It is expected that the resonance between the capsule and the fluid flow due to the cooling water in HANARO's In-core will not occur. Also, the new bottom structure of a solid cone shape with 71 mm in diameter will be applicable to the material and special capsules in the future.

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Effects of Plant Growth regulators on Rapid in vitro Propagation of Camptotheca acuminata from Axillary Buds

  • Kang, Seung-Mi;Min, Ji-Yun;Park, Dong-Jin;Jeong, Mi-Jin;Song, Hyun-Jin;Heo, Chang-Mi;Moon, Hyun-Shik;Kim, Jong-Gab;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of agriculture & life science
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    • v.45 no.1
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    • pp.33-40
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    • 2011
  • An efficient method for the rapid micropropagation of Camptotheca acuminata from axillary buds was established by application of various plant growth regulators. Among various cytokinins, $0.5mg\;L^{-1}$ BA showed the best performance on shoot multiplication, number average multiple shoots up to 10.8. The propagated shoot cuttings in vitro were elongated on NN basal medium without plant growth regulators. The secondary multiple shoots were induced at the site of initially induced buds. Rooting was induced directly near the base of the shoot on half-strength NN medium containing $0.5mg\;L^{-1}$ of IBA, whereas high concentration of $1.0mgL^{-1}$ IBA could induce callus at the base of the shoot. The camptothecin content, anticancer compound of the micropropagated plants was contained in various tissues. Camptothecin contents were 1.8 and $2.5mg\;g^{-1}$ dry weight in stems from propagated in vitro and mother plant, respectively. This result may be used to develop strategies for large-scale propagation of elite C. acuminata trees.

Development of PKNU3: A small-format, multi-spectral, aerial photographic system

  • Lee Eun-Khung;Choi Chul-Uong;Suh Yong-Cheol
    • Korean Journal of Remote Sensing
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    • v.20 no.5
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    • pp.337-351
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    • 2004
  • Our laboratory originally developed the compact, multi-spectral, automatic aerial photographic system PKNU3 to allow greater flexibility in geological and environmental data collection. We are currently developing the PKNU3 system, which consists of a color-infrared spectral camera capable of simultaneous photography in the visible and near-infrared bands; a thermal infrared camera; two computers, each with an 80-gigabyte memory capacity for storing images; an MPEG board that can compress and transfer data to the computers in real-time; and the capability of using a helicopter platform. Before actual aerial photographic testing of the PKNU3, we experimented with each sensor. We analyzed the lens distortion, the sensitivity of the CCD in each band, and the thermal response of the thermal infrared sensor before the aerial photographing. As of September 2004, the PKNU3 development schedule has reached the second phase of testing. As the result of two aerial photographic tests, R, G, B and IR images were taken simultaneously; and images with an overlap rate of 70% using the automatic 1-s interval data recording time could be obtained by PKNU3. Further study is warranted to enhance the system with the addition of gyroscopic and IMU units. We evaluated the PKNU 3 system as a method of environmental remote sensing by comparing each chlorophyll image derived from PKNU 3 photographs. This appraisement was backed up with existing study that resulted in a modest improvement in the linear fit between the measures of chlorophyll and the RVI, NDVI and SAVI images stem from photographs taken by Duncantech MS 3100 which has same spectral configuration with MS 4000 used in PKNU3 system.

Relating Hyperspectral Image Bands and Vegetation Indices to Corn and Soybean Yield

  • Jang Gab-Sue;Sudduth Kenneth A.;Hong Suk-Young;Kitchen Newell R.;Palm Harlan L.
    • Korean Journal of Remote Sensing
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    • v.22 no.3
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    • pp.183-197
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    • 2006
  • Combinations of visible and near-infrared (NIR) bands in an image are widely used for estimating vegetation vigor and productivity. Using this approach to understand within-field grain crop variability could allow pre-harvest estimates of yield, and might enable mapping of yield variations without use of a combine yield monitor. The objective of this study was to estimate within-field variations in crop yield using vegetation indices derived from hyperspectral images. Hyperspectral images were acquired using an aerial sensor on multiple dates during the 2003 and 2004 cropping seasons for corn and soybean fields in central Missouri. Vegetation indices, including intensity normalized red (NR), intensity normalized green (NG), normalized difference vegetation index (NDVI), green NDVI (gNDVI), and soil-adjusted vegetation index (SAVI), were derived from the images using wavelengths from 440 nm to 850 nm, with bands selected using an iterative procedure. Accuracy of yield estimation models based on these vegetation indices was assessed by comparison with combine yield monitor data. In 2003, late-season NG provided the best estimation of both corn $(r^2\;=\;0.632)$ and soybean $(r^2\;=\;0.467)$ yields. Stepwise multiple linear regression using multiple hyperspectral bands was also used to estimate yield, and explained similar amounts of yield variation. Corn yield variability was better modeled than was soybean yield variability. Remote sensing was better able to estimate yields in the 2003 season when crop growth was limited by water availability, especially on drought-prone portions of the fields. In 2004, when timely rains during the growing season provided adequate moisture across entire fields and yield variability was less, remote sensing estimates of yield were much poorer $(r^2<0.3)$.

Optimized cultivation of Ettlia sp. YC001 in eutrophic pond water for nutrient removal and biomass production

  • Oh, Hyung-Seok;Ahn, Chi-Yong;Srivastava, Ankita;Oh, Hee-Mock
    • ALGAE
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    • v.33 no.4
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    • pp.319-327
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    • 2018
  • Ettlia sp. YC001, a highly settleable and productive microalga, was shown to be effective in removing nutrients and capturing suspended solids from eutrophic pond water. The optimum conditions for the Ettlia sp. YC001 cultivation were investigated using water from a landscape pond. The pond water was supplemented with different N : P ratios by weight, and the biomass production and nutrient removal compared in batch cultures. The maximum removal rate of N and P was with an N : P ratio of 16 : 1. Plus, the turbidity dropped to near zero within 4 days. Meanwhile, chemostat cultivation showed that the biomass productivity and nutrient removal rate increased when increasing the dilution rate, where a dilution rate of $0.9d^{-1}$ showed the highest N and P removal rate at $32.4mg\;L^{-1}\;d^{-1}$ and $1.83mg\;L^{-1}\;d^{-1}$, respectively, and highest biomass and lipid productivity at $0.432g\;L^{-1}\;d^{-1}$ and $67.8mg\;L^{-1}\;d^{-1}$, respectively. The turbidity was also reduced by 98% in the chemostat cultivation. Moreover, auto-flocculation and pH were closely connected to the turbidity removal. As a result, this study identified the optimal N : P ratio for small pond water treatment using an Ettlia sp. YC001, while also establishing the optimal conditions for nutrient removal, turbidity reduction, and biomass production.

Passive 3D motion optical data in shaking table tests of a SRG-reinforced masonry wall

  • De Canio, Gerardo;de Felice, Gianmarco;De Santis, Stefano;Giocoli, Alessandro;Mongelli, Marialuisa;Paolacci, Fabrizio;Roselli, Ivan
    • Earthquakes and Structures
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    • v.10 no.1
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    • pp.53-71
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    • 2016
  • Unconventional computer vision and image processing techniques offer significant advantages for experimental applications to shaking table testing, as they allow the overcoming of most typical problems of traditional sensors, such as encumbrance, limitations in the number of devices, range restrictions and risk of damage of the instruments in case of specimen failure. In this study, a 3D motion optical system was applied to analyze shake table tests carried out, up to failure, on a natural-scale masonry structure retrofitted with steel reinforced grout (SRG). The system makes use of wireless passive spherical retro-reflecting markers positioned on several points of the specimen, whose spatial displacements are recorded by near-infrared digital cameras. Analyses in the time domain allowed the monitoring of the deformations of the wall and of crack development through a displacement data processing (DDP) procedure implemented ad hoc. Fundamental frequencies and modal shapes were calculated in the frequency domain through an integrated methodology of experimental/operational modal analysis (EMA/OMA) techniques with 3D finite element analysis (FEA). Meaningful information on the structural response (e.g., displacements, damage development, and dynamic properties) were obtained, profitably integrating the results from conventional measurements. Furthermore, the comparison between 3D motion system and traditional instruments (i.e., displacement transducers and accelerometers) permitted a mutual validation of both experimental data and measurement methods.

Analysis of End-Plated Propellers by Panel Method (패널법에 의한 날개끝판부착 프로펠러의 해석)

  • C.S. Lee;I.S. Moon;Y.G. Kim
    • Journal of the Society of Naval Architects of Korea
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    • v.32 no.4
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    • pp.55-63
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    • 1995
  • This paper describes the procedure to analyze the performance of the end-plated propeller(EPP) by a boundary integral method. The screw blade(SB) and end-plate(EP) are represented by a set of quadrilateral panels, where the source and normal dipole of uniform strength are distributed. The perturbation velocity potential, being the only unknown via the potential-based formulation, is determined by satisfying the flow tangency condition on the blade and the end-plate at the same time. The Kutta condition is satisfied through an iterative process by requiring the null pressure jump across the upper and lower sides of the trailing edges of both the SH and the EP. Sample calculations indicate that the EP increases the loading near the tip of the SB while spreading the trailing vortices along the trailing edge of the EP, thus avoiding the strong tip-vortex formation. Predicted performance of the EPP shows good correlations with the experimental results. The method is therefore considered applicable in designing and analyzing the EPP which may be an alternative for energy-saving propulsive devices.

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Physico-chemical and mineralogical study of ancient mortars used in Harran area (Turkey)

  • Binici, Hanifi;Akcan, Mehmet;Aksogan, Orhan;Resatoglu, Rifat
    • Advances in concrete construction
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    • v.5 no.6
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    • pp.639-658
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    • 2017
  • Very limited studies have been accomplished concerning the historical structures around Harran area. Collected mortar samples from the historic structures in the area were tested to explore their mechanical, chemical and mineralogical properties. Mortar samples from three different points of each historical structure were taken and specified in accordance with the related standards taking into consideration their mechanical, chemical and mineralogical properties. By means of SEM-EDX the presence of organic fibres and calcite, quartz, plagioclase and muscovite minerals has been examined. Additionally, by means of XRF analysis, oxide ($SiO_2$, $Al_2O_3$, and $Fe_2O_3$) percentages of mortar ingredients have been specified, also. According to the test results obtained, it was confirmed that the mortars had densities ranging between $1.51-2.10g/cm^3$, porosity values ranging between 8.89-35.38% and compressive strengths ranging between 5.02-5.90 MPa. Specimen HU, which has the highest durability and lowest water absorption and porosity, was the mortar taken from the most intact building in the mosque complex. This result is most likely due to the very little fine aggregate content of HU. In contrast, HUC mortars with a small amount of fine particles and brick contents yielded slightly lower compressive strengths. The interesting point of this study is the mineralogical analysis results and especially the presence of ettringite in these historic mortars linked to the use of pozzolanic materials. Survival of these historic structures in Harran Area through centuries of use and, also, having been subjected to many earthquakes can probably be explained by these properties of the mortars.