• Title/Summary/Keyword: G/2D

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Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]-1 Complex

  • Im, Yu-Jin;Jung, Sang-Mi;Kang, Ye-Song;Kim, Ho-Tae
    • Bulletin of the Korean Chemical Society
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    • v.34 no.4
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    • pp.1232-1236
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    • 2013
  • The Cu cation binding sites of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex have been investigated to explain the $[Cu{\cdot}DNA]$ biological activity caused by the Cu association to DNA. The structure of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex was investigated by electrospray ionization mass spectrometry (ESI-MS). The fragmentation patterns of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex were analyzed by MS/MS spectra. In the MS/MS spectra of $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex, three fragment ions were observed with the loss of d(CpG), {d(CpG) + Cyt}, and {d(CpG) + Cyt + dR}. The Cu cation binds to d(CpG) mainly by substituting the $H^+$ of phosphate group. Simultaneously, the Cu cation prefers to bind to a guanine base rather than a cytosine base. Five possible geometries were considered in the attempt to optimize the $[Cu(I){\cdot}d(CpG){\cdot}d(CpG)-2H]^{-1}$ complex structure. The ab initio calculations were performed at B3LYP/6-31G(d) level.

Access Control for D2D Systems in 5G Wireless Networks

  • Kim, Seog-Gyu;Kim, Jae-Hyun
    • Journal of the Korea Society of Computer and Information
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    • v.26 no.1
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    • pp.103-110
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    • 2021
  • In this paper, we compare two access control mechanisms for D2D(Device-to-Device) systems in 5G wireless networks and propose an effective access control for 5G D2D networks. Currently, there is no specified access control for 5G D2D networks but there can be two access control approaches for 5G D2D networks. One is the UE-to-Network Relay based access control and the other is the Remote UE(User Equipment) based access control. The former is a UE-to-Network Relay carries out the access control check for 5G D2D networks but the latter is a Remote UE performs the access control check for 5G D2D networks. Through simulation and evaluation, we finally propose the Remote UE based access control for D2D systems in 5G wireless networks. The proposed approach minimizes signalling overhead between the UE-to-Network Relay and the Remote UE and more efficiently performs the access control check, when the access control functionalities are different from the UE-to-Network Relay in 5G D2D networks.

Ultraviolet (UV)-B Irradiation Increased Vitamin D2 Contents in the Fruit Bodies of Pleurotus eryngii var. ferulae (자외선(UV)-B 조사에 의한 아위느타리버섯(Pleurotus eryngii var. ferulae) 자실체의 비타민 D2 함량 증가)

  • Rho, Jae-Young;Park, Sang-Don
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.191-194
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    • 2013
  • The fresh fruit bodies of Pleurotus eryngii var. ferulae was irradiated with ultraviolet (UV)-B (280-320 nm) in order to increase vitamin $D_2$ contents, which was assayed using HPLC (Waters 1525, USA). The vitamin $D_2$ contents were $3.5{\mu}g/g$ after 3 min UV-B irradiation ($21.6KJ/m^2$) and $6.02{\mu}g/g$ after 5 min UV-B irradiation ($36KJ/m^2$), respectively, which showed the significant increase considering the vitamin $D_2$ content ($0.01{\mu}g/g$) before UV-B irradiation. This increasing effect was confirmed also for other edible mushrooms; Pleurotus eryngii, from $0.09{\mu}g/g$ to $2.75{\mu}g/g$ (3 min) and $5.21{\mu}g/g$ (5 min); Lentinus edodes, from $0.021{\mu}g/g$ to $3.02{\mu}g/g$ (3 min) and $3.78{\mu}g/g$ (5 min); Pleurotus ostreatus, from $0.19{\mu}g/g$ to $9.63{\mu}g/g$ (3 min) and $11.6{\mu}g/g$ (5 min). Although the original content of vitamin $D_2$ was the highest in P. ostreatus, the extent of increase by UV irradiation was remarkably high in P. eryngii var. ferulae.

Seasonal Change of Rice-mediated Methane Emission from a Rice Paddy under Different Water Management and Organic Amendments (물 관리와 유기물 시용이 다른 논에서 벼 식물체를 통한 메탄 배출의 계절변화)

  • Shin, Yong-Kwang;Lee, Yang-Soo;Ahn, Jong-Woong;Koh, Mun-Hwan;Eom, Ki-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.1
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    • pp.41-49
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    • 2003
  • Methane emission was measured in a rice paddy under different water management and organic amendments. Methane emission from planted chambers and unplanted chambers was monitored to evaluate the rice-mediated methane emission. In flooding methane emission from planted chambers with NPK, NPK(+P), was $0.174g\;CH_4\;m^{-2}\;d^{-1}$ while that from unplanted chambers with NPK, NPK(-P), was $0.046g\;CH_4\;m^{-2}\;d^{-1}$ Methane emission from planted chambers with rice straw compost amendment, RSC(+P), was $0.214g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw compost amendment, RSC(-P), was $0.076g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in Fehruary, RS2(+P), was $0.328g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chambers with rice straw amendment in February, RS2(-P), was $0.1g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from planted chambers with rice straw amendment in May, RS5(+P), was $0.414g\;CH_4\;m^{-2}\;d^{-1}$, while that from unplanted chamhers with rice straw amendment in May, RS5(-P), was $0.187g\;CH_4\;m^{-2}\;d^{-1}$. In intermittent irrigation methane emission from NPK(+P) was $0.115g\;CH_4\;m^{-2}\;d^{-1}$, while that from NPK(-P) was $0.041g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RSC(+P) was $0.137g\;CH_4\;m^{-2}\;d^{-1}$, while that from RSC(-P) was $0.06g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS2(+P) was $0.204g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS2(-P) was $0.09g\;CH_4\;m^{-2}\;d^{-1}$. Methane emission from RS5(+P) was $0.273g\;CH_4\;m^{-2}\;d^{-1}$, while that from RS5(-P) was $0.13g\;CH_4\;m^{-2}\;d^{-1}$. Methane transport via rice plant under flooding for NPK plot, RSC plot, RS2 plot and RS5 plot was 73.6%, 64.5%, 69.5% and 54.8%, respectively, and mean was 65.6%. Methane transport via rice plants under intermittent irrigation for NPK plot, RSC plot, RS2 plot and RS5 plot was 64.3%, 59.2%, 55.9% and 52.4%, respectively, and mean was 58.0%.

Optimization of Culture Conditions for D-Tagatose Production from D-Galactose by Enterobacter agglomerans. (Entrobacter agglomerans에 의한 D-Galactose로부터 D-Tagatose 생산조건의 최적화)

  • 오덕근;노회진;김상용;노봉수
    • Microbiology and Biotechnology Letters
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    • v.26 no.3
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    • pp.250-256
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    • 1998
  • D-Tagatose production from D-galactose was investigated using 35 type strains of American Culture Type Collection (ATCC) and Korean Collection for Type Cultures (KCTC) which have potential to produce D-tagatose. Enterobacter agglomerans ATCC 27987 was selected as a D-tagatose producing strain due to its short fermentation time and high production of D-tagatose. Optimization of the culture conditions for D-tagatose production by E. agglomerans ATCC 27987 was performed. Among various carbon sources, D-galactose was the most effective carbon source for D-tagatose production. As the D-galactose concentration was increased, cell growth and D-tagatose production increased. Effect of nitrogen sources on D-tagatose production was studied. Of inorganic nitrogen sources, ammonium sulfate was effective one for D-tagatose production and yeast extract was the most suitable organic nitrogen nutrient. The concentrations of inorganic compounds such as KH$_2$PO$_4$, K$_2$HPO$_4$, and MgSO$_4$$.$7H$_2$O were also optimized for D-tagatose production. The optimal medium was determined to contain D-galactose of 20 g/l, yeast extract of 5.0 g/l, (NH$_4$)$_2$SO$_4$ of 2.0 g/l, KH$_2$PO$_4$ of 5.0 g/l, K$_2$HPO of 5.0 g/l, and MgSO$_4$$.$7H$_2$O of 5 mg/l. The optimal environmental conditions in a 250-$m\ell$ flask were found to be pH of 6.0, temperature of 30$^{\circ}C$, and agitation speed of 150 rpm. D-tagatose of 0.41 g/l could be obtained in 24 h from 20 g/l D-galactose at the optimal culture condition without induction and cell concentration.

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Effect of Neighbor Base Sequences on the Base Pair Stabilities at d(CXG) and d(GXC) in Human ε-globin Promoter (사람의 ε-글로빈 프로모트에서 d(CXG)와 d(GXC)의 안정성에 인접한 염기 서열들의 영향 에 관한 연구)

  • Chung, In-Ae;Gang, Jong-Back
    • Journal of Life Science
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    • v.12 no.2
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    • pp.208-212
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    • 2002
  • Human $\varepsilon$-globin DNA fragment was used to determine the thermal stabilities of base pairs at d(CXG) and d(GXC) by Temperature Gradient Gel Electrophoresis(TGGE). The base pair stability depends on the hydrogen bonding interaction and base stacking interaction of neighbor base sequence. The orders of base pair stabilities were T.AG.A = A.G>C.T>T.C>C.A>A.C for d(GXC).d(GYC).

Development of a Flood Model GUI using Open Source Software (오픈소스 소프트웨어를 이용한 침수해석 모형 GUI 개발)

  • Choi, Yun-Seok;Park, Sang Hoon;Kim, Joo Hun;Kim, Kyung-Tak
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.372-372
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    • 2019
  • 본 논문에서는 격자 기반의 2차원 침수해석 모형인 G2D(Grid based 2-Dimensional land surface flood model)의 GUI 개발에 대해서 기술하였다. G2D 모형은 ASCII 래스터 포맷의 DEM을 이용하여 정형 사각격자로 구성되는 침수모의 도메인을 설정하고, 수위, 수심, 유량 등의 경계조건과 강우와 유량을 연속방정식의 생성항으로 사용하여 2차원 침수모의를 한다. 주요한 침수모의 결과는 ASCII 래스터 포맷을 가지는 수심과 수위 등이다. 이와 같이 G2D 모형은 ASCII 래스터 파일을 주로 이용하고 있다. 본 연구에서는 우선 래스터 파일의 전후처리와 침수모의 결과의 가시화에 대한 편의성을 높이기 위해서 GIS 소프트웨어를 이용하여 GUI를 개발하고자 하였다. 이와 더불어 사용자들이 소프트웨어 구매 비용에 대한 부담을 없애고, 편리하게 사용할 수 있는 오픈소스 소프트웨어를 이용하고자 하였으며, 이 두 가지 조건을 만족할 수 있는 QGIS를 이용해서 G2D 모형의 GUI인 QGIS-G2D를 개발하였다. QGIS-G2D는 QGIS의 plug-in으로 실행된다. QGIS-G2D는 G2D 모형의 실행에 필요한 프로젝트 파일(.g2p)을 GUI를 이용해서 만들 수 있으며, 모의결과를 애니매이션 등으로 가시화 할 수 있는 후처리 기능을 포함하고 있다. 또한 QGIS-G2D는 DEM 수정 기능과 같이 G2D 모형의 입력자료 전처리를 위해서 QGIS plug-in으로 제공되는 여러 가지 기능을 함께 이용할 수 있다. 또한 물리적 분포형 강우-유출 모형인 GRM(Grid based Rainfall-runoff Model)의 QGIS plug-in인 QGIS-GRM과 연계하여, 유역 유출모의와 침수모의를 QGIS 환경에서 함께 수행할 수도 있다. 개발된 소프트웨어는 오픈소스 플랫폼인 GitHub(https://github.com/floodmodel/)를 통해서 제공된다. 본 연구를 통해서 홍수해석에 필요한 강우-유출 모의와 침수모의를 위한 모형을 제공하고, 이를 편리하게 활용할 수 있는 오픈소스 소프트웨어를 제공할 수 있었다. 이러한 연구들은 홍수 분야의 전문가들에 의해서 다양한 분야의 홍수해석에 사용될 수 있을 것으로 기대한다.

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Effects of Media and Elicitor on Betalain and Phytolaccosides Production in Hairy Root Cultures of Phytolacca esculenta van Houtte (자리공(Phytolacca esculenta van Houtte) 모상근의 Betalain 및 Phytolaccosides 생성에 미치는 배지 및 Elicitor 영향)

  • 양덕조;김용해;윤길영
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.6
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    • pp.507-514
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    • 1998
  • The effects of media and elicitors on betalain, phytolaccoside G and D2 production were examined in the hairy roots of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Phytolaccoside G and D2 from Phytolacca hairy roots PEH2 were identified by TLC, HPLC, IR, Mass, $^1$H-NMR, and ^(13)C-NMR. Among the culture media tested, SH medium was the best for hairy root growth of hairy roots. White medium was the most suitable medium for betalain production, while MS medium was for phytolaccoside G and D2 production. Although the growth of hairy roots was supped by light (1,000 Lux), the production of betalain, phytolaccoside G and D2 was enhanced by the same light treatment. Addition of elicitors such as NaF, chitosan, and yeast extract to the culture medium increased the content of betalain, phytolaccoside G and D2, suggesting the importance of culture condition for the production of those componds.

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Determinations of Ergocalciferol and Cholecalciferol in Mushrooms (버섯의 Ergocalciferol(Vit D$_2$)과 Cholecalciferol(Vit D$_3$)의 함량 측정)

  • 이진실;안령미;최희숙
    • Korean journal of food and cookery science
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    • v.13 no.2
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    • pp.173-178
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    • 1997
  • The contents of ergocalcife.of (vit D$_2$) and cholecalciferol (vit D$_3$) in mushrooms (Lentinus edodes, Agaricus bisporus, Pleurotus ostreatus, Flammulina velutipes, Auricularia auricular, Gyropora esculenta, Romaria botftis, Coriorus versicolar, Ganoderma lucidum) were determined by high-performance liquid chromatography (HPLC), using external standard method. The methods included saponification, extraction, drying, filtering and quantification with analytical HPLC (waters Inc.). The contents of vit D$_2$ and D$_3$ found in different mushroom species. A. auricular, and L. edodes contained high amounts of vit D, 167.8, 72.6 $\mu\textrm{g}$/100 g of edible portion, respectively. On the other hand A. bisporus showed the lowest contents of vit D among analyzed mushrooms.

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Cooperative Priority-based Resource Allocation Scheduling Scheme for D2D Communications Underlaying 5G Cellular Networks (5G 셀룰러 네트워크 하의 D2D통신을 위한 협력적 우선순위 기반의 자원할당 스케줄링)

  • Lee, Chong-Deuk
    • Journal of Digital Convergence
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    • v.18 no.10
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    • pp.225-232
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    • 2020
  • The underlaying communication scheme in 5G cellular network is a very promising resource sharing scheme, and it is an effective scheme for improving service performance of 5G and reducing communication load between a cellular link and a device to device (D2D) link. This paper proposes the algorithm to minimize the resource interference that occurs when performing 5G-based multi-class service on gNB(gNodeB) and the cooperative priority-based resource allocation scheduling scheme (CPRAS) to maximize 5G communication service according to the analyzed control conditions of interference. The proposed CPRAS optimizes communication resources for each device, and it optimizes resource allocation according to the service request required for 5G communication and the current state of the network. In addition, the proposed scheme provides a function to guarantee giga-class service by minimizing resource interference between a cellular link and a D2D link in gNB. The simulation results show that the proposed scheme is better system performance than the Pure cellular and Force cellular schemes. In particular, the higher the priority and the higher the cooperative relationship between UE(User Equipment), the proposed scheme shows the more effective control of the resource interference.