• 대한전자공학회논문지TE
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• 제39권1호
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• pp.83-90
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• 2002

본 논문은 2인용 보드게임의 정보에 대한 전략을 학습할 수 있는 방법을 역전파 신경회로망과 Q학습알고리즘을 이용하여 제안하였다. 학습의 과정은 단순히 상대프로세스와의 대국에 의하여 이루어진다. 시스템의 구성은 탐색을 담당하는 부분과 기물의 수를 발생하는 부분으로 구성되어 있다. 수의 발생부분은 보드의 상태에 따라서 갱신되고, 탐색커널은 αβ 탐색을 기본으로 역전파 신경회로망과 Q학습을 결합하여 게임에 대해 양호한 평가함수를 학습하였다. 학습의 과정에서 일련의 기물의 이동에 있어서 인접한 평가치들의 차이만을 줄이는 Temporal Difference학습과는 달리, 기물의 이동에 따른 평가치에 대해 갱신된 평가치들을 이용하여 평가함수를 학습함으로써 최적의 전략을 유도할 수 있는 Q학습알고리즘을 사용하였다. 일반적으로 많은 학습을 통하여 평가함수의 정확도가 보장되면 승률이 학습의 양에 비례함을 알 수 있었다.

• 한국콘텐츠학회논문지
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• 제10권7호
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• pp.88-97
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• 2010

최근 유비쿼터스 기술의 발달과 도시의 패러다임 변화에 따라 효율적인 광고의 정보 전달을 위하여 첨단 기술과 매체간 융합을 통해 버스의 도착시간안내, 스마트폰을 활용한 광고 등 도시민들에게 더욱더 편리한 서비스를 제공하고 있다. 그러나 뉴미디어를 이용한 실시간 이동형광고의 경우 대용량 데이터의 전달방법과 통신료 부담 등의 어려움이 많아 이를 개선시키는 방법이 필요하다. 본 논문은 이러한 어려움을 극복하고자 연구 되었다. 이동형광고 중 u-버스광고 비즈니스 모델과 이동시 실시간 정보제공, 대용량 데이터전송 등 매체의 특성 분석을 통한 차별화 사업전략 방안을 제시하며, 운영센타에서 네트워크를 통해 차량 단말부를 통한 광고송출까지 구체적인 시스템 구축방안을 제시한다. 본 논문은 첨단기술과 다양한 매체와의 융합을 통한 실무적으로 활용 가능한 비즈니스모델로서 시사점을 제공한다는 측면에서 큰 의의가 있다.

• BMB Reports
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• 제45권6호
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• pp.331-336
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• 2012

The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.

• Biomolecules & Therapeutics
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• 제22권2호
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• pp.114-121
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• 2014

Refractoriness of acute myeloid leukemia (AML) cells to chemotherapeutics represents a major clinical barrier. Suicide gene therapy for cancer has been attractive but with limited clinical efficacy. In this study, we investigated the potential application of herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) based system to inhibit chemoresistant AML cells. We first generated Ara-C resistant K562 cells and doxorubicin-resistant THP-1 cells. We found that the HSV-TK/GCV anticancer system suppressed drug resistant leukemic cells in culture. Chemoresistant AML cell lines displayed similar sensitivity to HSV-TK/GCV. Moreover, HSV-TK/GCV killing of leukemic cells was augmented to a mild but significant extent by all-trans retinoic acid (ATRA) with concomitant upregulation of Connexin 43, a major component of gap junctions. Interestingly, HSV-TK/GCV killing was enhanced by expression of vesicular stomatitis virus G glycoprotein (VSV-G), a fusogenic membrane protein, which also increased leukemic cell fusion. Co-culture resistant cells expressing HSV-TK and cells stably transduced with VSV-G showed that expression of VSV-G could promote the bystander killing effect of HSV-TK/GCV. Furthermore, combination of HSV-TK/GCV with VSV-G plus ATRA produced more pronounced antileukemia effect. These results suggest that the HSV-TK/GCV system in combination with fusogenic membrane proteins and/or ATRA could provide a strategy to mitigate the chemoresistance of AML.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2004년도 Proceedings of the 4th Korea-China Joint Workshop on Nuclear Waste Management
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• pp.220-237
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• 2004

China, as a developing country with a great number of population and relatively less energy resources, reasonably emphasizes the nuclear energy utilization development. For the long term sustainable energy supply, as for nuclear application the basic strategy of PWR-FBR-Fusion has been settled and envisaged. Due to the economy and experience reasons the nuclear power and technology development with a moderate style are kept in China up to now. In China mainland apart from two NPPs with the total capacity of 2.1 GWe in operation, four NPPs are under construction and two NPPs are planned for the Tenth Five Year Plan(2001-2005). Also another one or two NPPs are still in discussion. It could be foreseen that the total nuclear power capacity will reach 8.5GWe before the year 2005 and 14-15 GWe before 2010 respectively. As the first step for the Chinese fast reactor engineering development the 65MWt China Experimental Fast Reactor(CEFR) is under construction. The main components of primary, secondary and tertiary circuits and of fuel handling system have been ordered. The reactor building under construction has reached the top namely 57m above the ground. More than one hundred components and shielding doors have been installed. It is planned that the construction of reactor building with about 40,000$m^2$ floor surface will be completed in the end of the year 2002 and envisaged that the first criticality of the CEFR will be in the end of 2005. The second step of the Chinese fast reactor engineering development is a 300MWe Prototype Fast Breeder Reactor which is only under consideration up to now. Some important technical selections have been settled, but its design has not yet started.

• 한국동물생명공학회지
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• 제35권3호
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• pp.215-222
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• 2020

Mesenchymal stem cells (MSCs) have been widely used as donor cells for somatic cell nuclear transfer (SCNT) to increase the efficiency of embryo cloning. Since replicative senescence reduces the efficiency of embryo cloning in MSCs during in vitro expansion, transfection of telomerase reverse transcriptase (TERT) into MSCs has been used to suppress the replicative senescence. Here, TERT-transfected MSCs in comparison with early passage MSCs (eMSCs) and sham-transfected MSCs (sMSCs) were used to evaluate the effects of embryo cloning with SCNT in a porcine model. Cloned embryos from tMSC, eMSC, and sMSC groups were indistinguishable in their fusion rate, cleavage rate, total cell number, and gene expression levels of OCT4, SOX2 and NANOG during the blastocyst stage. The blastocyst formation rates of tMSC and sMSC groups were comparable but significantly lower than that of the eMSC group (p < 0.05). In contrast, tMSC and eMSC groups demonstrated significantly reduced apoptotic incidence (p < 0.05), and decreased BAX but increased BCL2 expression in the blastocyst stage compared to the sMSC group (p < 0.05). Therefore, MSCs transfected with telomerase reverse transcriptase do not affect the overall development of the cloned embryos in porcine SCNT, but enables to maintain embryo quality, similar to apoptotic events in SCNT embryos typically achieved by an early passage MSC. This finding offers a bioengineering strategy in improving the porcine cloned embryo quality.

• 한국콘텐츠학회논문지
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• 제9권5호
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• pp.118-127
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• 2009

현재 전 세계적으로 공동주거문화의 확산과 국내 신규 분양 아파트 환경의 연속적인 증가 추세가 각 주거공간과 세대간에 홈 네트워크 기기의 수요를 증대시키고 있다. 유비쿼터스의 계속적 진화와 홈 네트워크 시스템 도입과 발전이 제품 디자인의 수요를 촉진시키고 있다. 주거문화의 변화와 아파트 실내디자인의 변화는 생활자의 라이프 스타일과 제품의 디자인에 상호 영향을 미치고 있으며, 새로운 제품디자인을 요구하고 있다. 실내 제품과의 조화, 인테리어 스타일과 제품, 제품과 패션디자인, 제품과 제품간의 요소적인 인자의 재구성과 융합이 본 디자인 연구의 핵심적 요소이다. 이것을 바탕으로 디자인 프로세스는 통합적인 디자인 전략과 컨셉트의 조사와 분석으로 연구되었으며, 디자인의 기능성, 외관의 심미적인 디자인이 일치될 수 있는 3차원 디자인으로 표현하였다. 실질적인 디자인 안을 발표함으로서 향후 발전 전망의 단면을 구체적으로 예측할 수 있기 때문이다.

• 한국기술혁신학회:학술대회논문집
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• 한국기술혁신학회 2003년도 춘계학술대회
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• pp.413-425
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• 2003

Information Technology is the kernel technology deciding the industrial standard of one nation, and biotechnology will be the main technology of next generation. Based on this fact, a lot of efforts were made to industrialize them. Nano Technology is beginning to position itself as the kernel fusion technology, and its usage and popularity is expanding. Environmental and Energy Technology is a must-have strategic technology considering the increase demand of new energy development, the international environment correspondence, the environment-friendly production, and so forth. Space Technology is the field, which will contribute to raise the domestic component and system technology to the next level. In 2001, new technology research development costs total of 1 trillion 32 billion won in the following fields; 437.82 billion won in IT, 88.457 billion won in BT, 46.799 billion won in NT, 315.682 billion won in ET, and 112 billion won in ST. from component ratio, IT forms 42% which is the most, 31% for ET and in order of BT, ST and NT. ETRI and KISTI are concentrating on IT, KIBB is on BT, KAERI, KIER, KERI and KBSI are focusing on ET, and KIMM, KRISS, KRICT and KORDI is participating together in 4∼5 new technology such as IT, BT, NT and ET. Funds for research development costs in 5 new technology fields of 13 contribution (year) are consisted as follows; The Office for Government Policy Coordination has contributed 131 billion won (13%), 387 billion won (37%) by MOST, 256 billion won (25%) by Ministry of Information and Communication, 67 billion won (6%) by Ministry of Commerce, Industry and Energy, 19% by others and the industrial world. < Strategy for Technology Advancing > o Promotion of comprehensive contributing (year) new technology development research plan project o Increase research efficiency by promoting new technology development project connected with peculiar projects of organization by contribution (year) o Formation of superior research group by technology and introduction of operation system for research accumulation are needed. o Technology demand-oriented assignment deduction and promotion of research development project connected with intermediate long term objective o National will and investment extension of research development costs, training and popularization of professionals, commercialization promotion with efficient control for research plan and result.

• 기술혁신연구
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• 제22권4호
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• pp.117-143
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• 2014

기존의 기술 진화 경로 연구는 주로 거시적 동향 분석 수준에서 이루어져 왔으며 포괄적인 기술정책 방향 수립에는 시사점이 있었으나, 기업 기술 및 특허전략에는 활용가치가 낮았다. 2000년대부터 논문 및 특허 등의 데이터를 활용해 미시적인 기술의 진화 경로를 분석하고, 기업 기술전략에 적용하려는 연구가 증가하고 있다. 그러나 대부분 과거 진화 경로의 서술에 그치고 있으며 기술의 진화 또는 파생-융합 등의 변화에 대한 분석은 전문가의 정성적 판단에 상당부분 의존하고 있다. 본 연구에서는 특허 인용 네트워크를 구축해 미시적 기술의 진화 경로를 도출하고, 기술의 진화와 파생을 동적 기술트리를 통해 분석하는 방법론을 제시한다. 동적 기술트리 분석은 기술의 핵심요소를 체계화하고, 신기술 요소의 신규성과 확장성을 평가해 차세대 기술진화와 파생에 대한 정량적 판단을 가능하게 한다. 이를 통해 차세대 신기술과 파생기술에 대하여 파악, 평가, 비교하고 나아가 예측의 토대를 구축한 것이 본 연구의 의의이다. 본 연구의 결과는 기술 및 특허전략과 포트폴리오 구축의 신뢰성을 높일 수 있는 좋은 도구가 될 것이다. 본 연구에서 제안한 방법론을 이용하여 최근 전력산업에서 기존 교류 송전의 대안으로 주목을 받고 있는 초고압 직류송전 시스템 기술을 대상으로 실증분석을 수행하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.173-177
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• 2006

In this study, a novel strategy was developed for the highly selective immobilization of proteins, using the polyhydroxyalkanoate (PHA) depolymerase substrate binding domain (SBD) as an active binding domain. In order to determine the appropriacy of this method for immunodiagnostic assays, the single-chain antibody (ScFv) against the hepatitis B virus (HBV) preS2 surface protein and the severe acute respiratory syndrome coronavirus (SARS-CoV) envelope protein (SCVe) were fused to the SBD, then directly immobilized on PH A-coated slides via microspotting. The fluorescence-labeled HBV antigen and the antibody against SCVe were then utilized to examine specific interactions on the PHA-coated surfaces. Fluorescence signals were detected only at the spotted positions, thereby indicating a high degree of affinity and selectivity for their corresponding antigens/antibodies. Furthermore, we detected small amounts of ScFv-SBD (2.7 ng/mL) and SCVe-SBD fusion proteins (0.6ng/mL). Therefore, this microarray platform technology, using PHA and SBD, appears generally appropriate for immunodiagnosis, with no special requirements with regard to synthetic or chemical modification of the biomolecules or the solid surface.