• Title/Summary/Keyword: Fusion device

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Expression and Purification of GFPuv/Cytochrome c-552 Fusion Protein in E. coli

  • Hong, Eul-Jae;Lee, Sang-On;Choe, Jeong-U;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.550-553
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    • 2003
  • The genes of GFPuv and Cytochrome c-552 were amplified by using PCR, and then, fused each other. Fusion gene of GFPuv and Cytochrome c-552 was inserted into the pTrcHis B vector and transferred to E. coli. A fusion protein of GFPuv and Cytochrome c-552 was expressed in JM109 and BL21. This fusion protein was composed of a His-tag for the rapid one-step purification using an immobilized metal affinity chromatography.

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Investigation on High Transmittance Vertical Alignment (VA) display associated with Surface Polymer Stabilization (표면 고분자 안정화를 이용한 고 광효율 VA 디스플레이 연구)

  • Kim, Woo-Il;Kim, Sung-Min;Cho, In-Young;Kim, Dae-Hyun;Kwon, Dong-Won;Jeong, Yeon-Hak;Ryu, Jae-Jin;Kim, Kyeong-Hyeon;Lee, Seung-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.39-40
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    • 2009
  • The patterned vertical alignment (PVA) display showed good electro-optic properties such as wide viewing angle, fast response time, high Contrast Ratio. However, the device has patterned electrode on both top and bottom electrodes which requires high accuracy of assembling of top and bottom substrates in order to exhibit a high performance. So, they have disadvantage about low yield. In order to resolve these problem, in this paper, we studied about top substrate pattern free vertical alignment device associated with surface polymer stabilization using the UV curable monomer. This method shows simple progress, low cost and good electro-optic properties such as high transmittance and fast response time.

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p-contact resistivity influence on device-reliability characteristics of GaN-based LEDs (p-contact 저항에 따른 GaN기반 LED의 device-reliability 특성)

  • Park, Min-Jung;Kim, Jin-Chul;Kim, Sei-Min;Jang, Sun-Ho;Park, Il-Kyu;Park, Si-Hyun;Cho, Yong;Jang, Ja-Soon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.159-159
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    • 2010
  • We conducted bum-in test by current stress to evaluate acceleration reliability characteristics about p-resistivity influence of GaN-based light-emitting diodes. The LEDs used in this study are the polarization field-induced LED(PF-LED) having low p-resistivity and the highly resistive LED(HR-LED) having high p-resistivity. The result of high stress experiment shows that current crowding phenomenon is occurred from the center of between p-bonding pad and n-bonding pad to either electrodes. In addition, series resistance and optical power decrease dramatically. These results means that the resistance of between p-bonding pad and p-GaN affect reliability. That's why we need to consider the ohmic contact of p-bonding pad when design the high efficiency and high reliability LEDs.

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Controls on KSTAR Superconducting Poloidal Field (PF) Magnets

  • Hahn, Sang-Hee;Kim, K.H.;Choi, J.H.;Ahn, H.S.;Lee, D.K.;Park, K.R.;Eidietis, N.W.;Leuer, J.A.;Walker, M.L.;Yang, H.L.;Kim, W.C.;Oh, Y.K.
    • Progress in Superconductivity and Cryogenics
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    • v.10 no.4
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    • pp.23-28
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    • 2008
  • As a part of the plasma control system (PCS) for the first plasma campaign of KSTAR, seven sets of fast feedback control loop for the superconducting poloidal field magnet power supply (PF MPS) have been implemented. A special real-time digital communication interface has been developed for the simultaneous exchanges of the current/voltage data from the 7 sets of 12-thyristor power supplies in a 200 microsecond control cycle. Preliminary power supply tests have been performed before actual cooldown of the device. A $29mH/50m{\Omega}$ solenoid dummy has been fabricated for a series of single power supply tests. Connectivity and response speed of the plasma control system have been verified. By changing hardware cabling, this load was also used to estimate mutual inductance coupling effects of two geometrically adjacent solenoid coils on each power supply. After the cooldown was complete, each pair of the up/down symmetric PF coils has been serially connected and tested as part of the device commissioning process. Bipolar operation and longer pulse attempts have been investigated. The responses of the coils and power supplies corresponding to the plasma magnetic controls in plasma discharges are also analyzed for the future upgrades.

A Basic Study for the Fabrication of Micro Cell-Fusion-Device (초소형 세포융합기구의 제작을 위한 기초연구 (원형질체 준비 및 전계인가방식에 관한 연구를 중심으로))

  • Lee, Sang-Hoon;Cha, Hyoun-Chul;Kim, Young-Uk;Kim, Young-Kweon
    • Proceedings of the KIEE Conference
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    • 1993.07a
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    • pp.287-289
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    • 1993
  • Recently semiconductor technology is widely used in biotechnology fields and one of the represetatives is electric cell-fusion-device. In this paper. some basic researches required in the development of this fusion system was performed and they are as follows: 1) preparation of protoplast and selection of the fittest plant cell, 2) development of high frequency AC power supply for cell-alignment by using class-E resonant circuit. As a result, cabbage cell is selected and pearl-chain, which shows cell-alignment. can be observed by the applied AC field.

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A Vehicle Recognition Method based on Radar and Camera Fusion in an Autonomous Driving Environment

  • Park, Mun-Yong;Lee, Suk-Ki;Shin, Dong-Jin
    • International journal of advanced smart convergence
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    • v.10 no.4
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    • pp.263-272
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    • 2021
  • At a time when securing driving safety is the most important in the development and commercialization of autonomous vehicles, AI and big data-based algorithms are being studied to enhance and optimize the recognition and detection performance of various static and dynamic vehicles. However, there are many research cases to recognize it as the same vehicle by utilizing the unique advantages of radar and cameras, but they do not use deep learning image processing technology or detect only short distances as the same target due to radar performance problems. Radars can recognize vehicles without errors in situations such as night and fog, but it is not accurate even if the type of object is determined through RCS values, so accurate classification of the object through images such as cameras is required. Therefore, we propose a fusion-based vehicle recognition method that configures data sets that can be collected by radar device and camera device, calculates errors in the data sets, and recognizes them as the same target.

Design and Manufacture of Fusion Sensor Mechanism (융합센서 기구 설계 및 제작)

  • Shin, Seong-Yoon;Cho, Gwang-Hyun;Cho, Seung-Pyo;Shin, Kwang-Seong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.10a
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    • pp.418-419
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    • 2022
  • In this paper, the design of the fusion sensor mechanism was divided into primary and secondary design, and the device design and case design were divided to facilitate the safety of the sensor, sensor coupling, and connection with power.

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Investigating the effect of changing parameters in the IEC device in comparative study

  • H. Ghammas;M.N. Nasrabadi
    • Nuclear Engineering and Technology
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    • v.56 no.1
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    • pp.292-300
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    • 2024
  • Kinetic simulations have been performed on an Inertial Electrostatic Confinement Fusion (IECF) device. These simulations were performed using the particle-in-cell (PIC) method to analyze the behavior of ions in an IEC device and the effects of some parameters on the Confinement Time (CT). CT is an essential factor that significantly contributes to the IEC's performance as a nuclear fusion device. Using the PIC method, the geometry of a two-grided device with variable grid radius, the number of cathode grid rings, variable pressure and different dielectric thickness for the feed stalk was simulated. In this research, with the development of previous works, the interaction of particles was simulated and compared with previous results. The simulation results are in good agreement with the previous results. In these simulations, it was found that with the increase of the dielectric thickness of the feed stalk, the electric field was weakened and as a result, the confinement time was reduced. On the other hand, with the increase of the cathode radius, the confinement time increased. Using the results, an IEC device can be designed with higher efficiency and more optimal CT for ions.

Risk Factors and Surgical Treatment for Symptomatic Adjacent Segment Degeneration after Lumbar Spine Fusion

  • Cho, Kyoung-Suok;Kang, Suk-Gu;Yoo, Do-Sung;Huh, Pil-Woo;Kim, Dal-Soo;Lee, Sang-Bok
    • Journal of Korean Neurosurgical Society
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    • v.46 no.5
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    • pp.425-430
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    • 2009
  • Objective : The present study analyzed the risk factors, prevalence and clinical results following revision surgery for adjacent segment degeneration (ASD) in patients who had undergone lumbar fusion. Methods : Over an 8-year period, we performed posterior lumbar fusion in 81 patients. Patients were followed a minimum of 2 years (mean 5.5 years). During that time, 9 patients required revision surgery due to ASD development. Four patients underwent autogenous posterolateral arthrodesis and extended transpedicle screw fixation, 4 patients underwent decompressive laminectomy and interspinous device implantation, and 1 patient underwent simple decompression. Results : Of the 9 of patients with clinical ASD, 33.3% (3 of 9) of patients did not have radiographic ASD on plain radiographs. Following revision surgery, the clinical results were excellent or good in 8 patients (88.9%). Age > 50 years at primary surgery was a significant risk factor for ASD development, while number of fusion levels, initial diagnosis and type of fusion were not. Conclusion : The incidence of ASD development after lumbar surgery was 11.1% (9 of 81) in this study. Age greater than 50 was the statistically significant risk factor for ASD development. Similar successful clinical outcomes were observed after extended fusion with wide decompression or after interspinous device implantation. Given the latter procedure is less invasive, the findings suggest it may be considered a treatment alternative in selected cases but it needs further study.

Cylindrical Object Recognition using Sensor Data Fusion (센서데이터 융합을 이용한 원주형 물체인식)

  • Kim, Dong-Gi;Yun, Gwang-Ik;Yun, Ji-Seop;Gang, Lee-Seok
    • Journal of Institute of Control, Robotics and Systems
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    • v.7 no.8
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    • pp.656-663
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    • 2001
  • This paper presents a sensor fusion method to recognize a cylindrical object a CCD camera, a laser slit beam and ultrasonic sensors on a pan/tilt device. For object recognition with a vision sensor, an active light source projects a stripe pattern of light on the object surface. The 2D image data are transformed into 3D data using the geometry between the camera and the laser slit beam. The ultrasonic sensor uses an ultrasonic transducer array mounted in horizontal direction on the pan/tilt device. The time of flight is estimated by finding the maximum correlation between the received ultrasonic pulse and a set of stored templates - also called a matched filter. The distance of flight is calculated by simply multiplying the time of flight by the speed of sound and the maximum amplitude of the filtered signal is used to determine the face angle to the object. To determine the position and the radius of cylindrical objects, we use a statistical sensor fusion. Experimental results show that the fused data increase the reliability for the object recognition.

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