• Title/Summary/Keyword: Function Separation

Search Result 631, Processing Time 0.027 seconds

Nuclear Bodies Built on Architectural Long Noncoding RNAs: Unifying Principles of Their Construction and Function

  • Chujo, Takeshi;Hirose, Tetsuro
    • Molecules and Cells
    • /
    • v.40 no.12
    • /
    • pp.889-896
    • /
    • 2017
  • Nuclear bodies are subnuclear, spheroidal, and membraneless compartments that concentrate specific proteins and/or RNAs. They serve as sites of biogenesis, storage, and sequestration of specific RNAs, proteins, or ribonucleoprotein complexes. Recent studies reveal that a subset of nuclear bodies in various eukaryotic organisms is constructed using architectural long noncoding RNAs (arcRNAs). Here, we describe the unifying mechanistic principles of the construction and function of these bodies, especially focusing on liquid-liquid phase separation induced by architectural molecules that form multiple weakly adhesive interactions. We also discuss three possible advantages of using arcRNAs rather than architectural proteins to build the bodies: position-specificity, rapidity, and economy in sequestering nucleic acid-binding proteins. Moreover, we introduce two recently devised methods to discover novel arcRNA-constructed bodies; one that focuses on the RNase-sensitivity of these bodies, and another that focuses on "semi-extractability" of arcRNAs.

Effect of X-ray Exposure on Phase Separation of Lysozyme-Water Mixture (X선 쬐임이 단백질-물 상분리에 미치는 영향)

  • Cho, Chang-Ho;Sung, Syng-Hoon;Lee, Sang-Soo;Cho, Kun-woo
    • The Journal of Natural Sciences
    • /
    • v.11 no.1
    • /
    • pp.23-26
    • /
    • 1999
  • Phase separation temperature of lysozyme-water mixture increased with X-ray exposure on lysozyme and decreased with impurity of saponin. The intensity of light scattering in lysozyme-water mixture with X-ray exposure on lysozyme decreased as a function as a function of temperature, and decreased with impurity of saponin.

  • PDF

Study on the Electro-Optic Characteristics Depending on UV Condition in the Normally Scattering(NS)/Normally Transparent(NT) Polymer Dispersed Liquid Crystal Mode (Normally Scattering(NS)/Normally Transparent (NT) 고분자 분산형 액정 모드에서 UV 조건에 따른 전기 광학특성 연구)

  • Kim, M.S.;Seo, Y.H.;Lee, M.H.;Rhee, J.M.;Lee, T.S.;Lee, S.H.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2003.07b
    • /
    • pp.1050-1053
    • /
    • 2003
  • Polymer dispersed liquid crystal mode can be classified into two cases, normally scattering mode (NS) and normally transparent (NT) mode according to an initial state. Electro-optic characteristic NS/NT PDLC mode can be improved by controlling the factors to influence the phase separation. In case of NS PDLC mode, we inspected change of a phase separation as a function of curing temperature for the mixture of E7 and NOA65 with different ratios. When the LC concentration is less than polymer such as LC : NOA65 = 40 : 60wt%, the PDLC cell is influenced strongly by the curing temperature. However, when LC concentration is much less than polymer such as LC : NOA65 : 80 : 20wt%, it is influenced slightly by the curing temperature. In case of NT PDLC mode, we observed change of a phase separation as a function of the ratio of the mixture and UV conditions such as curing temperature, UV intensity The cell made with strong UV intensity and curing temperature of $20^{\circ}C$, in case that the ratio of LC to polymer is 70:30wt%, showed good electro-optic characteristics.

  • PDF

Design and Implementation of test system for safety separation and performance verification of aircraft weapons/equipment (항공기 무장/장착물의 안전 분리 및 성능 검증을 위한 점검 시스템 설계 및 구현)

  • Kim, Hyo-joung;Kim, Yang-won;Kwon, Byung-Gi
    • Journal of Advanced Navigation Technology
    • /
    • v.26 no.2
    • /
    • pp.47-53
    • /
    • 2022
  • With the recent development of defense technology, various aircraft armaments/equipments are being developed. Among them, cruise guided weapons(missile) for launching aircraft can be installed on aircraft aftersecuring reliability and safety through varioustests. In order to avoid collision with aircraft, missile separated from aircraft must release restraint of wing at the specified time, control wing in the specified direction. In order to confirm this, a test system that can control MIL-STD-1760 according to safety separation procedure and verify release performance is required. test system needs a function to check circuit for release of restraints and a function to check driving performance by controlling MIL-STD-1760. In addition, test system should be simulate environment separated from aircraft. This paper presented a testsystemthat can verify circuit and driving performance mounted on missile according to the safety separation procedure, and it was confirmed that it was normally separated from aircraft through flight tests.

An Analysis on Achievement-assessing Style for Serious Game (기능성게임의 성취도 평가 유형 분석)

  • Yoon, Seon-Jeong
    • Journal of Korea Game Society
    • /
    • v.12 no.1
    • /
    • pp.15-24
    • /
    • 2012
  • Serious games are purposive game. So game-developers want to achieve the desired purposes, and game-users also want to play to achieve the goals of playing games. The function of achievement-assessing is an important area of serious game. In this study, achievement-assessing style was defined as three types, that is, 'all-in-one', 'complex', and 'separation'. Accordingly, we analyzed which type of serious games use which type of achievement-assessing style. As a result, the survey shows that those who want to play game itself preferred 'all-in-one' above all, and those who want to stick to the purpose of game preferred 'separation' or 'complex'. In addition, the investigation of preference of achievement-assessing function shows that users, as a whole, preferred 'all-in-one'. We hope this study will be useful data to design of achievement-assessing function which achieve the purpose of game and provide users with higher satisfaction.

Effect of internal stress on cyclic fatigue failure in K3 (K3에 축적된 내부 응력이 피로 파절에 미치는 영향)

  • Kim, Jun-Young;Kim, Jin-Woo;Cho, Kyung-Mo;Park, Se-Hee
    • Restorative Dentistry and Endodontics
    • /
    • v.37 no.2
    • /
    • pp.74-78
    • /
    • 2012
  • Objectives: This study aimed to evaluate the relationship between the cyclic fatigue of a K3 file and internal stress intentionally induced until the activation of the autostop function of the torque-controlled motor. Materials and Methods: K3 (Sybron Endo) .04 and .06 taper, size 25, 30, 35, 40 and 45 were used in this study. To give the internal stress, the K3 files were put into the .02 taper Endo-Training-Bloc (Dentsply Maillefer) until the activation of the auto-stop function of the torque-controlled motor. The rotation speed was 300 rpm and torque value was 1.0 $N{\cdot}cm$. K3 were grouped by the number of induced internal stress and randomly distributed to 4 experimental groups (n = 10, Stress 0 [control], Stress 1, Stress 2 and Stress 3). For measuring the cyclic fatigue failure, the K3 files were worked against a sloped glass block and time for file separation was recorded. Data was statistically analyzed Statistical analyses were performed using two-way ANOVA and Duncan post-hoc test at p < 0.05 level. Results: Except .04 taper size 30 in Stress 1 group, there were statistically significant differences in time for file separation between control and all experimental groups. K3 with .04 taper showed higher cyclic fatigue resistance than those of .06 taper. Conclusion: In the limitation of this study, the cyclic fatigue of the K3 file was influenced by the accumulated internal stress from use until the auto-stop function was activated by the torque-controlled motor. Therefore, clinicians should avoid the reuse of the K3 file that has undergone auto-stops.

An Analysis on the Geomagnetic Transfer Function at Yongin Observatory Using by RR (Remote Reference) and SNS (Signal Noise Separation) Technique (원격참조(RR: Remote Reference) 기법과 신호잡음분리(SNS: Signal Noise Separation)기술을 이용한 용인 관측소의 지자기 전달함수 분석)

  • Yang Junmo;Lee Duk-Kee;Kwon Byung-Doo;Ryu Yong-Gyu;Youn Yong-Hoon
    • Economic and Environmental Geology
    • /
    • v.38 no.2 s.171
    • /
    • pp.155-163
    • /
    • 2005
  • For an unbiased TF (Tansfer Function) estimations we investigate geomagnetic TF derived from ICHEON and YONGIN sites, employing RR (Remote Reference) and SNS (Signal Noise Separation) techniques. The Rh technique, which requires synchronized field variations recorded at a clean remote site, is a reliable method to minimize the bias of TF by uncorrelated noises in magnetic channels. Meanwhile, SNS technique based on the assumption of noise-free remote data can improve the signal-noise level by separating signal TF and noise TF, which is successfully applied to the environments with strong correlated noises. In this study, TF at YONGIN is analyzed using geomagnetic data from ICHEON site as a remote reference, which seem to have somewhat better data quality. The application of Rh technique reduces the bias of TF, which appears in single site robust estimation, and makes curves in the amplitude and phase of TF more smooth as frequency. Futhermore, in order to investigate noise source quantitatively, SNS technique is applied. The results of SNS suggest that dominant noise source seems to be located at western region of YONGIN. This noise source is considered to originate from railway system such as KTX and national subway. which passes through the west regions of YONGIN.