• 한국해안·해양공학회논문집
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• 제28권1호
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• pp.44-52
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• 2016

항주파 파봉의 위치를 예측하는 Kelvin(1887)의 이론해는 심해에서만 적용 가능한 한계가 있다. 최대파향각을 예측하는 Havelock(1907)의 이론해는 모든 수심에서 적용 가능하지만 파향각에 따라 다르게 나타나는 파봉의 위치를 예측하지 못하는 한계가 있다. 본 연구에서 항주파의 분산관계식을 온전하게 이용하여 모든 수심에 적용 가능한 항주파 파봉식을 개발하고, 이 식을 이용하여 항주파의 최대파향각을 예측하였다. FLOW-3D를 이용하여 Johnson(1958)의 수리모형실험을 수치적으로 재현한 후 본 연구에서 제안하는 최대파향각의 이론해가 모든 수심에서 수치해, 수리실험결과와 유사함을 확인하였다. 여러 조건에서 항주파를 수치적으로 재현한 후 파봉선 간의 거리를 측정하여 이론해와 비교하였다. 그 결과 선박의 속도가 ${\sqrt{gh}}$보다 작은 경우 이론해와 유사하였다. 선박의 속도가 ${\sqrt{gh}}$보다 큰 경우 첫 번째 항주파의 거리를 결정하는 상수 $C_1$은 0에 가까운 값이었고 첫 번째 항주파를 제외한 경우 이론해와 유사하였다.

• 한국수자원학회논문집
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• 제45권1호
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• pp.39-52
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• 2012

유역내에서 발생하는 유출은 지표 유출과 지표하 유출이 있으며, 서로 상호작용 상태를 유지하게 된다. 일반적으로 지표와 지표하 둘 중 한 가지 알고리즘으로 해석이 힘든 유역에 대해 지표와 지표하 사이의 동적인 관계를 상세하게 모의해야 하는 경우 상호작용에 관한 요소를 고려하여야 한다. 동적인 상호작용 시스템의 구동에서는 시 공간적인 매개변수가 중요하며, 적절한 모의를 위해시 공간적인 매개변수는 시스템 상에서 지표와 지표하 항에 대한 복합적인 메카니즘으로 구성되어야 한다. 본 연구에서는 이러한 지표 및 지표하 유출의상호작용에 관한 알고리즘을 위해 2차원 확산파 방정식을 이용하여 지표 유출을 해석하고, Darcy의 법칙과 Dupuit-Forchheimer의 가정을 이용한 Boussinesq 방정식을 적용하여 포화상태의 지표하 유출의 알고리즘을 구성하였다. 커플링 방정식으로 공간에 대해서는 유한체적법을 사용하고, 시간에 대해서는 Crank-Nicolson 방법을 이용하였으며, 지표와 지표하 흐름의 상호작용에 대해서는 질량보존의 법칙에 기반하여 구성하였다. 이상의 과정을 통하여 지표 유출해석, 지표하 유출해석, 상호작용, 수치해석 부분의 4가지 주요 모듈을 만들었으며, 4가지 주요 모듈을 통합하여 복합유역의 지표 및 지표하 유출해석 모듈을 개발하였다.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.347-355
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• 2020

기상이변으로 인한 식물 스트레스를 평가하기 위해 토양과 식물에 적용할 수 있는 여러 센서가 개발되었다. 따라서 본 연구의 목적은 식물의 온도 스트레스를 평가하기 위해 다양한 센서를 사용하여 기후 및 토양 상태와 식물 반응을 모니터링하는 것이다. 식물 반응을 평가하기 위한 식물 센서로 식물 줄기의 전기전도도, sap flow rate를 모니터링하였다. 식물 줄기의 전기전도도는 물과 이온 수송을 포함한 식물의 생리적 활성을 반영한다. 완전히 자란 Brassica oleracea var. italica를 온도 20/15 ℃ (낮/밤), 16시간 광주기로 하여 대조구로 설정하였으며 저온 15/10 ℃ 및 고온 35/30 ℃에 노출시키면서 토양 및 식물 상태를 모니터링하였다. 식물 줄기의 전기전도도, sap flow rate는 낮에는 증가하고 밤에는 감소하였다. 저온 스트레스 하에서 Brassica oleracea var. italica 줄기의 전기전도도는 대조구보다 낮았고, 고온 스트레스 하에서는 대조구보다 높아 물과 이온 수송이 온도에 의해 영향을 받았음을 나타낸다. 그러나 엽록소 a와 b 함량은 저온 스트레스를 받은 잎에서 증가했고 고온 스트레스를 받은 잎에서는 대조구와 차이가 없었다. 잎의 프롤린 함량은 저온 스트레스에서는 대조구와 차이가 없는 반면, 고온 스트레스에서는 증가했다. 프롤린 합성은 환경 스트레스 하에서 식물의 방어 메커니즘으로 작용한다. 따라서 Brassica oleracea var. Italica는 저온보다 고온 스트레스에 더 민감한 것으로 판단된다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 추계학술대회 초록집
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• pp.111.1-111.1
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• 2010

This paper focuses on a systematic configuration of steam reforming fuel processor, particularly designed for small and medium sized hydrogen production application. In a typical integration of the fuel processor, there exist significant temperature gradients over the entire system which has negative effect on both catalyst life-time and system performance. Also, the volumetric inefficiency should be avoided to obtain the possible compactness for the commercial purpose. In the present work, the computational analysis will be performed to gain the fundamental insight on the transport phenomena and chemical reactions in the reformer consisting of preheating, steam reforming (SR), and water gas shift (WGS) reaction beds in the flow direction. Also, the fuel processing system includes a top-fired burner providing necessary thermal energy for endothermic catalytic reactor. A fully two-dimensional numerical modeling for a integrated fuel processing system is introduced for in-depth analysis of the heat and mass transport phenomena based on surface kinetics and catalytic process. In the model, water gas shift reaction and decomposition reaction were assumed to be at equilibrium. A kinetic model was developed and then computational results were compared with the experimental data available in the literature. Finally, the case study was done by considering the key parameters, i.e. steam to carbon (S/C) ratio and temperature. The computer-aided models developed in this study can be greatly utilized for the design of advanced fast-paced compact fuel processors research.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.243-247
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• 2004

The dynamometers which had made in a long time ago could not control the input/output quantity of water minutely and was sensitive to a noise since it was controlled by an analog control method. Therefore, a fully digital controlled system was urgently required to be robust against various noises. In this paper, the new system which can control the amount of circulated water in dynamometer was developed. This system is consisted of an industrial digital type controller and a servo motor. The industrial PLC was used as a main controller for the developed system, and the actuator and servo motor were used to control the inlet and outlet valve independently. The torque signal of load cell was fed back to the main controller to regulate the diesel engines load. Generally, an input/output valve position of the old dynamometer was fixed with a proper situation for an engine output test and the torque was changed according to the time interval. However, the torque value for the dynamometer could not be constantly kept because of the variation of the input water flow and fluid characteristic. Therefore, the automatic control of an inlet and outlet valve should be performed to keep the constant torque. So, the PID control method was applied to solve this problem. Also, the development of a web-based remote control system was described in this paper. This software will give us the convenience of operation, the more efficient operations, and the reduced operator workload for operation of the dynamometer. The application results of the system have been verified at actual diesel engine field.

• 소성∙가공
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• 제15권9호
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• pp.667-672
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• 2006

Agglutination is one of the most commonly employed reactions in clinical diagnosis. In this paper, we have designed and fabricated nickel mold insert for injection molding of a microfluidic lab-on-a-chip for the purpose of the efficient detection of agglutination. In the presented microfluidic lab-on-a-chip, two inlets for sample blood and reagent, flow guiding microchannels, improved serpentine laminating micromixer(ISLM) and reaction microwells are fully integrated. The ISLM, recently developed by our group, can highly improve mixing of the sample blood and reagent in the microchannel, thereby enhancing reaction of agglutinogens and agglutinins. The reaction microwell was designed to contain large volume of about $25{\mu}l$ of the mixture of sample blood and reagent. The result of agglutination in the reaction microwell could be determined by means of the level of the light transmission. To achieve the cost-effectiveness, the microfluidic lab-on-a-chip was realized by the injection molding of COC(cyclic olefin copolymer) and thermal bonding of two injection molded COC substrates. To define microfeatures in the microfluidic lab-on-a-chip precisely, the nickel mold inserts of lab-on-a-chip for the injection molding were fabricated by combining the UV photolithography with a negative photoresist SU-8 and the nickel electroplating process. The microfluidic lab-on-a-chip developed in this study could be applied to various clinical diagnosis based on agglutination.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.193-196
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• 2008

The vortical structures in a turbulent boundary layer (TBL) developed over a flat plate have been investigated experimentally. The flow conditions tested in this study were Re$_{\theta}$ = 3700, Re$_{\delta}$ = 11${\times}$105 and the shape factor H = 1.3. Instantaneous velocity fields in the streamwise-wall-normal planes were measured by using a dynamic PIV system. A trip-wire and sandpapers were placed behind the leading edge to promote the turbulent transition. 1000 velocity fields were obtained consecutively with a time interval of 1 millisecond. Streamwise u-velocity components were temporally averaged in the measuring plane. In addition, 2000 velocity fields were obtained randomly and ensemble-averaged to get the fully-developed turbulent characteristics. Profiles of the normalized u-component, turbulent intensities and Reynolds shear stress were evaluated. The structures of spanwise vortices were extracted from the instantaneous velocity fields by determining the swirling strength, ${\lambda}_{ci}$. The wall-normalized locations of vortices were temporally averaged in the measuring plane with respect to their rotational direction. The correlations between the temporally averaged u and the temporally averaged $y^+$ of vortices were evaluated. For the case of positive vortices, the correlation is not significant. However, the negative vortices show a strong negative correlation. The y-location of negative vortices tends to increase, as the averaged u decreases and vice versa. These findings indicate that the number of negative vortices in the outer layer increases during the outward bursting events.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2006년도 추계학술대회
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• pp.65-74
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• 2006

Modem drug discovery requires rapid pharmacokinetic evaluation of chemically diverse compounds for early candidate selection. This demands the development of analytical methods that offer high-throughput of samples. Naturally, liquid chromatography / tandem mass spectrometry (LC-MS/MS) is choice of the analytical method because of its superior sensitivity and selectivity. As a result of the short analysis time(typically 3-5min) by LC-MS/MS, sample preparation has become the rate- determining step in the whole analytical cycle. Consequently tremendous efforts are being made to speed up and automate this step. In a typical automated 96-well SPE(solid-phase extraction) procedure, plasma samples are transferred to the 96-well SPE plate, internal standard and aqueous buffer solutions are added and then vacuum is applied using the robotic liquid handling system. It takes only 20-90 min to process 96 samples by automated SPE and the analyst is physically occupied for only approximately 10 min. Recently, the ultra-high flow rate liquid chromatography (turbulent-flow chromatography)has sparked a huge interest for rapid and direct quantitation of drugs in plasma. There is no sample preparation except for sample aliquotting, internal standard addition and centrifugation. This type of analysis is achieved by using a small diameter column with a large particle size(30-5O ${\mu}$m) and a high flow rate, typically between 3-5 ml/min. Silica-based monolithic HPLC columns contain a novel chromatographic support in which the traditional particulate packing has been replaced with a single, continuous network (monolith) of pcrous silica. The main advantage of such a network is decreased backpressure due to macropores (2 ${\mu}$m) throughout the network. This allows high flow rates, and hence fast analyses that are unattainable with traditional particulate columns. The reduction of particle diameter in HPLC results in increased column efficiency. use of small particles (<2 urn), however, requires p.essu.es beyond the traditional 6,000 psi of conventional pumping devices. Instrumental development in recent years has resulted in pumping devices capable of handling the requirements of columns packed with small particles. The staggered parallel HPLC system consists of four fully independent binary HPLC pumps, a modified auto sampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The process of Bioanalytical Method Validation is required by the FDA to assess and verify the performance of a chronlatographic method prior to its application in sample analysis. The validation should address the selectivity, linearity, accuracy, precision and stability of the method. This presentation will provide all overview of the work required to accomplish a full validation and show how a chromatographic method is suitable for toxirokinetic sample analysis. A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method developed to quantitate drug levels in dog plasma will be used as an example of tile process.

• 한국조경학회지
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• 제44권5호
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• pp.103-115
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• 2016

본 연구는 낙동정맥의 녹색에어컨 기능을 활용 확대하기 위해 낙동정맥의 찬공기 생성과 흐름에 대한 특징을 분석하고, 낙동정맥의 중요한 찬공기 생성지역 보호 및 인근 도시로의 찬공기 활용 극대화를 위한 관리방안을 제안하였다. 이를 위해, 낙동정맥 인근에 위치한 부산 구덕산 일대, 울산 고헌산 일대, 포항 운주산 일대를 대상지로 선정하여 정맥의 지형적 특징 및 토지이용에 따른 찬공기 흐름의 특징을 분석하였고, 이를 바탕으로 녹색 에어컨 기능 관리체계 구축을 위한 전략을 제시하였다. 부산 구덕산 일대는 정맥 경계에 인접한 지역에서의 고밀도 개발로 인해 생성된 찬공기가 충분히 발달하지 못하며, 시가지로 확산되지 못하고 있다. 향후 정맥 접경지역에 대한 개발 압력이 매우 커질 것으로 예상되므로, 정맥의 녹색서비스 기능 강화를 위해 높은 보전정책이 요구된다. 울산 고헌산 일대는 넓은 계곡에 위치한 농공단지와 산업단지에는 충분히 발달된 찬공기가 원활하게 유입되고 있어 현재의 찬공기 유입을 유지할 수 있는 관리방안이 필요하며, 넓고 평평한 지형으로 인해 향후 개발이 확대될 가능성이 있기 때문에 보전이 반드시 필요하다. 마지막으로 포항 운주산 일대는 복잡한 산악 및 계곡지형으로써 생성된 찬공기가 좁은 계곡을 통해 이동하면서 계곡 하단부로 갈수록 점차 발달하고 있으므로, 찬공기 생성지역 가까이에 위치한 계곡 지형을 활용해야 한다. 특히, 영천호 주변은 찬공기층이 가장 높으면서도 흐름이 가장 낮으므로 찬공기로 인한 피해를 예방하는 계획이 필요하다. 본 연구의 결과는 기후변화에 대응하기 위한 낙동정맥의 체계적인 관리계획을 수립하는데 기초자료로 활용될 수 있으며, 향후 녹색에어컨 기능산지의 양적 확대 및 보전을 위한 정맥관리 강화 등 산지의 녹색에어컨 기능을 확대하는 정책 마련에 활용될 수 있을 것으로 기대한다.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.253-261
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• 2007

Molecular and cultivation techniques were used to characterize the bacterial communities of biobead reactor biofilms in a sewage treatment plant to which an Aerated Up-Flow Biobead process was applied. With this biobead process, the monthly average values of various chemical parameters in the effluent were generally kept under the regulation limits of the effluent quality of the sewage treatment plant during the operation period. Most probable number (MPN) analysis revealed that the population of denitrifying bacteria was abundant in the biobead #1 reactor, denitrifying and nitrifying bacteria coexisted in the biobead #2 reactor, and nitrifying bacteria prevailed over denitrifying bacteria in the biobead #3 reactor. The results of the MPN test suggested that the biobead #2 reactor was a transition zone leading to acclimated nitrifying biofilms in the biobead #3 reactor. Phylogenetic analysis of 16S rDNA sequences cloned from biofilms showed that the biobead #1 reactor, which received a high organic loading rate, had much diverse microorganisms, whereas the biobead #2 and #3 reactors were dominated by the members of Proteobacteria. DGGE analysis with the ammonia monooxygenase (amoA) gene supported the observation from the MPN test that the biofilms of September were fully developed and specialized for nitrification in the biobead reactor #3. All of the DNA sequences of the amoA DGGE bands were very similar to the sequence of the amoA gene of Nitrosomonas species, the presence of which is typical in the biological aerated filters. The results of this study showed that organic and inorganic nutrients were efficiently removed by both denitrifying microbial populations in the anaerobic tank and heterotrophic and nitrifying bacterial biofilms well-formed in the three functional biobead reactors in the Aerated Up-Flow Biobead process.