• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.265-274
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• 1995

Characteristics of vegetative spur leaf, spur bud, fruiting supr leaf and flowering in relation to canopy position was investigated on order to promote better spur bud and flower bud differentiation on Citrus junos. There was no difference in vegetative spur leaf characteristics among directions. Vegetative spur leaf number and leaf area showed no difference between top and bottom position. There was no difference in spur bud size among directions, but spur bud at top was larger than that at bottom. Specific leaf weight of vegetative spur was mostly influenced by light interception, and leaf dry weight per spur average leaf dry weight, spur bud length and diameter had also a very high correlation with light interception rate. Shading and GA treatment in spur bud and flower bud differentiation was ineffective than natural light. Fruit thinning enhanced fruit quality as well as flower bud differentiation through an increase of leaf number per fruit, over 40 in Citrus junos.

• Journal of Ecology and Environment
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• v.41 no.9
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• pp.256-264
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• 2017

Background: The aim of this study was to clarify the relationship between the sexual reproduction and the resource allocation in a natural Polygonatum humile population grown in a temperate mixed forest gap. For this aim, the plant size, the node which flower was formed, the fruiting rate, and the dry weight of each organ were monitored from June 2014 to August 2015. Results: Firstly, in 3-13-leaf plants, plants with leaves ${\leq}8$ did not have flowers and in plants with over 9 leaves the flowering rate increased with the number of leaves. Among plants with the same number of leaves, the total leaf area and dry weight of flowering plants were larger than those of non-flowering plants. The minimum leaf area and dry weight of flowering plants were $100cm^2$ and 200 mg, respectively. Secondary, the flowers were formed at the 3rd~8th nodes, and the flowering rate was highest at the 5th node. Thirdly, cumulative values of leaf properties from the last leaf (the top leaf on a stem) to the same leaf rank were greater in a plant with a reproductive organ than in a plant without a reproductive organ. Fourthly, fruit set was 6.1% and faithful fruit was 2.6% of total flowers. Biomasses of new rhizomes produced per milligram dry weight of leaf were $0.397{\pm}190mg$ in plants that set fruit and $0.520{\pm}0.263mg$ in plants that did not, and the difference between the 2 plant groups was significant at the 0.1% level. Conclusions: P. humile showed that the 1st flower formed on the 3rd node from the shoot's base. And P. humile showed the minimum plant size needed in fruiting, and fruiting restricted the growth of new rhizomes. However, the fruiting rate was very low. Thus, it was thought that the low fruiting rate caused more energy to invest in the rhizomes, leading to a longer rhizome. A longer rhizome was thought to be more advantageous than a short one to avoid the shading.

• Korean Journal of Community Nutrition
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• v.20 no.1
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• pp.21-29
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• 2015

Objectives: Maintaining a balanced diet is very crucial for adolescents. However, adolescents, who may have a short notion about the amount of food, find it difficult to plan daily meals by applying the target pattern proposed by the Korean Nutrition Society. This study was carried out to revise the target pattern based on cooked dishes instead of raw material food groups as an easier way for Korean adolescents to plan their meals. Methods: Target pattern for Korean adolescents were revised based on the following: $1^{st}$, categorize dish groups, $2^{nd}$, calculate representative values of each dish based on the adolescent' intake amount. $3^{rd}$, assign the recommended number of intake for each dish. Validity of the target pattern for Korean adolescent meal plan was examined by the energy content, energy contribution ratio, and NAR & INQ of nutrients. Results: The 11 dish groups categorized were bab; gook tang gigae; side dishes of meat, fish, egg, legume, kimchi, vegetable, seaweed; and between meal of fruit, and milk dairy product. Based on the representative energy values, recommended number of intake were assigned to each dish. For boys, bab and gook tang gigae: 3 each; meat, fish, egg, and legume: 1 each; kimchi and vegetable: 3 each; seaweed: 1; fruit and milk dairy product: 2 each were assigned. For girls, bab and gook tang gigae: 2 each; meat, fish, egg, and legume: choice of 3 dishes, 1 each; kimchi and vegetable: 3 each; seaweed: 1; fruit and milk dairy product: 2 each were assigned. Energy contents, energy contribution ratio of carbohydrate, protein, and fat for boys and girls were in adequate range. The NARs were 1.0 and INQs were ${\geq}1.0$ for all nutrients examined. Conclusions: Revised dish-based, instead of food-based adolescent target patterns for boys and girls were easier and a valid way of Korean adolescent meal planning.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.815-826
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• 2018

The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.109-116
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• 2018

We investigated the effects of honey bee (Apis mellifera L.) colony size on the pollination of greenhouse-cultivated watermelon grown under the forcing cultivation system. The highest pollination activity of bees was observed ($14.3{\pm}5.0$ honey bees/day) when the bee colony size was 10,000 followed by 7,500 and 5,000 honey bees. There was a positive correlation between the bee colony size and pollination activity (R = 0.262) but insignificant difference in fruit set with different honey bee colony sizes (88%-91%). Evaluation of physical properties revealed that the weight and shape of watermelon were also not significantly different among different colony sizes. However, larger the bee colony size, higher the number of seeds were fertilized and rate of seed fertilization (p > 0.05). Number of seeds and content of sugar were negatively correlated (R = -0.714). Fertilized seeds showed a significant increase in mealy flesh, which has a negative effect on fruit quality, compared with that of the unfertilized seeds. Overall, we found that a colony size of 5,000 honey bees was the most effective for the pollination of watermelon grown under forcing cultivation. A comparison of the effects of bee pollination with those of artificial pollination suggested that artificial pollination can be effectively replaced by bee pollination in the forcing cultivation of watermelon, because fruit set, weight, and shape by bee pollination were similar to those achieved by artificial pollination.

• BMB Reports
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• v.37 no.3
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• pp.275-281
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• 2004

Rhodanese was isolated and purified from the cytosolic fraction of liver tissue homogenate of the fruit bat, Eidolon helvum, by using ammonium sulphate precipitation and CM-Sephadex C-50 ion exchange chromatography. The specific activity was increased 130-fold with a 53% recovery. The $K_m$ values for KCN and $Na_2S_2O_3$ as substrates were $13.5{\pm}2.2\;mM$ and $19.5{\pm}0.7\;mM$, respectively. The apparent molecular weight was estimated by gel filtration on a Sephadex G-100 column to be 36,000 Da. The optimal activity was found at a high pH (pH 9.0) and the temperature optimum was $35^{\circ}C$. An Arrhenius plot of the heat stability data consisted of two linear segments with a break occurring at $35^{\circ}C$. The apparent activation energy values from these slopes were 11.5 kcal/mol and 76.6 kcal/mol. Inhibition studies on the enzyme with a number of cations showed that $Mg^{2+}$, $Mn^{2+}$, $Ca^{2+}$, and $Co^{2+}$ did not affect the activity of the enzyme, but $Hg^{2+}$ and $Ba^{2+}$ inhibited the enzyme.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1084-1091
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• 2012

Xylooligosaccharides are functional foods mainly produced during the hydrolysis of xylan by physical, chemical, or enzymatic methods. In this study, production of xylobiose was investigated using oil palm empty fruit bunch fiber (OPEFB) as a source material, by chemical and enzymatic methods. Xylanase-specific xylan hydrolysis followed by xylobiose production was observed. Among different xylanases, xylanase from FXY-1 released maximum xylobiose from pretreated OPEFB fiber, and this fungal strain was identified as Aspergillus terreus and subsequently deposited under the accession Number MTCC- 8661. The imperative role of lignin on xylooligosaccharides enzymatic synthesis was exemplified with the notice of xylobiose production only with delignified material. A maximum 262 mg of xylobiose was produced from 1.0 g of pretreated OPEFB fiber using FXY-1 xylanase (6,200 U/ml) at pH 6.0 and $45^{\circ}C$. At optimized environment, the yield of xylobiose was improved to 78.67 g/100 g (based on xylan in the pretreated OPEFB fiber).

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1063-1066
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• 2016

Longan (Dimocarpus longan Lour.) has been used as a traditional oriental medicine and possesses a number of physiological activities. In this study, we used cell-based herbal extract screening to identify longan fruit extract (LFE) as an activator of osteoblast differentiation. LFE up-regulated alkaline phosphatase (ALP) activity, induced mineralization, and activated Runx2 gene expression in MC3T3-E1 cells. Furthermore, treatment of MC3T3-E1 cells with LFE promoted the phosphorylation of extracellular signal-regulated kinase1/2 (Erk1/2); however, abrogation of Erk1/2 activation with PD98059 resulted in down-regulation of the phospho-SMAD1/5/8 and Runx2 levels, which in turn reduced the ALP activity. Our findings suggest that LFE exerts its osteogenic activity through activation of the ERK signaling pathway and may have potential as an herbal therapeutic or a preventive agent for the treatment of osteoporosis.

• Korean Journal of Pharmacognosy
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• v.34 no.3 s.134
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• pp.206-209
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• 2003

The fruit of Evodia officinalis (Rutaceae) is known for containing a number of indoloquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of Evodiae Fructus. These alkaloids were isolated and determined by forming complex compounds from Evodiea Fructus. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate [II] complex ion. The absorbance of alkaloidal complex in 1.2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Evodia Fruit was linear over the concentration range of $1.0{\sim}6.0\;mg/ml$. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Evodiae Fructus.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.577-583
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• 2014

Benzyladenine (BA, 99% purity), MaxCel$^{(R)}$ (1.9% BA), Fruitone (3.5% NAA), MaxCel$^{(R)}$ + Fruitone, a nd s imazine were applied postbloom as fruitlet thinning agents to mature 'Hongro' and 'Fuji' apple (Malus domestica Borkh.) trees. BA and MaxCel$^{(R)}$ were applied at $100mg{\cdot}L^{-1}$ a.i. while Fruitone at $0.1mg{\cdot}L^{-1}$ a.i. and simazine at $400mg{\cdot}L^{-1}$ a.i. All PGRs were applied at 8 days after full bloom (DAFB, 6 mm fruit diameter) in both cultivars, while simazine was treated twice at 7 and 14 DAFB. In 'Hongro', the number of total fruit set per flower cluster in terminal buds was 1.67, 1.84, and 1.81 in MaxCel$^{(R)}$ + F ruitone, MaxCel$^{(R)}$, and simazine applications, respectively, when compared with 2.35 of water control. These reductions in fruit set were mainly attributed to the increased ratio of defruited clusters by the thinning agents. In 'Fuji' apple, the number of total fruit set per flower cluster in terminal buds was 1.29, 1.60, and 1.76 in MaxCel$^{(R)}$ + Fruitone, Fruitone, and MaxCel$^{(R)}$, respectively, when compared with 2.56 of water control in 'Fuji' apple. The addition of Fruitone to the MaxCel$^{(R)}$ promoted the thinning efficacy in both cultivars, compared to MaxCel$^{(R)}$ only. The thinning efficacies were similarly observed with lateral flowers in both cultivars. A significant increase of fruit weight by the postbloom thinning treatments was observed only in the BA application in 'Hongro', while the effect was observed in BA and MaxCel$^{(R)}$ in 'Fuji'. While the soluble solids content increased in the BA, MaxCel$^{(R)}$ and MaxCel$^{(R)}$+Fruitone treatments in both cultivars, other fruit quality attributes were not affected by the application of post-bloom thinning agents.