Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Longan (Dimocarpus longan Lour.) Fruit Extract Stimulates Osteoblast Differentiation via Erk1/2-Dependent RUNX2 Activation

  • Park, Seoyoung (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Kim, Joo-Hyun (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Son, Younglim (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Goh, Sung-Ho (Cancer Genomics Branch National Cancer Center) ;
  • Oh, Sangtaek (Department of Bio and Fermentation Convergence Technology, Kookmin University)
  • Received : 2016.02.01
  • Accepted : 2016.03.08
  • Published : 2016.06.28
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Abstract

Longan (Dimocarpus longan Lour.) has been used as a traditional oriental medicine and possesses a number of physiological activities. In this study, we used cell-based herbal extract screening to identify longan fruit extract (LFE) as an activator of osteoblast differentiation. LFE up-regulated alkaline phosphatase (ALP) activity, induced mineralization, and activated Runx2 gene expression in MC3T3-E1 cells. Furthermore, treatment of MC3T3-E1 cells with LFE promoted the phosphorylation of extracellular signal-regulated kinase1/2 (Erk1/2); however, abrogation of Erk1/2 activation with PD98059 resulted in down-regulation of the phospho-SMAD1/5/8 and Runx2 levels, which in turn reduced the ALP activity. Our findings suggest that LFE exerts its osteogenic activity through activation of the ERK signaling pathway and may have potential as an herbal therapeutic or a preventive agent for the treatment of osteoporosis.

Keywords

References

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