• 대한수의학회지
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• 제49권4호
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• pp.285-290
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• 2009

This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{\times}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.

• 한국가축번식학회지
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• 제11권1호
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• pp.22-25
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• 1987

This study was carried out to investigate the effects of imported pellet frozen semen on sperm motility and NAR acrosomes after thawing, and the farrowing rates, litter sizes and preweaning body weights after artificial insemination. A total of 28 sows of Landrace, Large White and Duroc were inseminated at the Chungnam Provincial Animal Breeding Station. The results obtained are summarized as follows: 1. Landrace andLarge White had higher sperm motility than Duroc by about 20% and had higher NAR acrosomes by about 10%. 2. The farrowing rates of Landrace, Large White and Duroc were 63.6, 55.6 adn 50.0%, respectively. The number of pigs born alive per litter were larger in Landrace and Large White as compared with Duroc (p<.01). 3. Duroc had the highest mean pig weight at birth, followed by Landrace and Large White (p<.01). The mean pig weights at 21 days and 56 days had no significant differences between the breeds.

• 한국가축번식학회지
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• 제8권2호
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• pp.87-91
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• 1984

This study was conducted to determine the optimum time and the insemination site of frozen in Cheju native cows, and 660 cows first service inseminated with rectovaginal methods by 3 skillful technicians as 3 districts near Cheju City and Sogwipo City areas obtained the following results. Optimum time for insemination was 16-20 hours after the onset of estrus and 80.6% of 67 conceived to first insemination. In the rectovaginal technique used widely at present conception rate for the palpation of ovaries showed 6.6% (index) higher than that of non-palpation. Conception rate of the insemination site; the intra-uterine horn, intra-uterus, just inside the cervix and depositing in mid-cervix were 62.5, 78.4, 74.6, and 80.9% respectively, however, there was no difference in conception rate between cervical and uterine deposition of forzon semen except for the intra-uterine horn.

• 한국가축번식학회지
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• 제17권2호
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• pp.127-132
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• 1993

Frozen semen obtained from 5 different Korean Native Cattle were used for in vitro fertilization of oocytes. The proportions(3∼11%) of oocytes penetrated in the basic medium with 13.9 mM glucose were very low in different semen. However, the penetration rates of oocytes in the presence of caffeine and heparin increased to 68∼84%. The proportions(10∼52%) of oocytes penetrated in the presence of caffeine alone were low at various glucose concentrations(0∼27.8 mM). In the medium with heparin, significantly(P<0.05 at least) higher penetration rate was obtained in the absence(94%) than in the presence(28∼45%) of 0∼27.8 mM glucose concentrations. The medium with caffeine and heparin, however, produced similar penetration rates(84∼97%) regardless of the various glucose concentrations.

• 한국수정란이식학회지
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• 제11권1호
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• pp.81-83
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• 1996

This study was carried out to investigate the effect of sperm concentration of 5ml maxi-straw on farrowing rate and number of pigs born alive per litter in deep frozen boar semen. We did not find out the effect of sperm concentration on post-thaw sperm motility and NAR acrosome. However, farrowing rate and number of pigs born alive per litter of 7. 5 x 10˚ /5ml and 10.0 x 10˚ /5m1 sperm concentrations were higher than those of 5. 0 /10˚ /5ml sperm concentration.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1276-1281
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• 2010

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than $3{\times}10^3$ sperm/ml were used for cryopreservation. The selected semen samples were then pooled and diluted 1:4 with Tris Citrate Fructose Yolk (TCFY) extender supplemented with different concentrations of BHT (0.5, 10, 2.0 and 3.0 mM). As the control, semen was diluted and frozen in the diluent without BHT. Motility, progressive motility, viability, membranes and acrosome integrity were evaluated after dilution (part 1), cooling (part 2) and freezing and thawing (part 3). The results of the first part of the experiment showed that there were no significant difference between treatments in the motility, progressive motility, viability, membranes and acrosome integrity of spermatozoa, but the results with 2.0 mM BHT were slightly better than obtained with other levels of BHT and control extender. Significantly better results (p<0.05) were observed in the second part of the experiment for cooled spermatozoa characteristics, when extender was supplemented with 2.0 and 3.0 mM BHT. Furthermore, the results obtained in the third part of the experiment indicated that, after freezing and thawing, all evaluated semen characteristics were improved significantly (p<0.05) by increasing BHT levels, with the best results obtained for extender containing 2 mM BHT. Comparison of these results with those of control diluent, the effects of supplementation were significantly (p<0.01) better. However, the higher concentration of BHT (3.0 mM) reduced the motility, acrosomal integrity, viability and hypo-osmotic swelling response of spermatozoa compared to extender containing 2.0 mM BHT. In conclusion, the results obtained in this study showed that the semen quality of rams was improved when BHT was added to extender used before the freezing process.

• 한국수정란이식학회지
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• 제25권4호
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• pp.237-245
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• 2010

Only an optimum number of viable spermatozoa in a frozen-thawed insemination dose can ensure conception at artificial insemination (AI). We report here the percentages of normal, abnormal and viable spermatozoa present in the frozen-thawed semen of 20 Black Bengal bucks used for commercial AI. Bucks in this experiment were of 19.3~46.1 months old and 25~42 kg body weight. Four semen straws (0.25 ml) from each buck were collected for evaluation of their kinetic parameters. Scrotal circumference was measured by using a scrotal tape, sperm motility was estimated on eye estimation and sperm concentration was determined by using a haemocytometer. Sperm morphology was studied in paraformaldehyde fixed spermatozoa under differential interference contrast (DIC) microscope. To determine the proportion of live (plasma membrane intact) spermatozoa, semen was stained with SYBR-14 and propidium iodide and examined under fluorescent microscope. Scrotal circumference, post-thaw sperm motility, sperm concentration per insemination dose and proportion of normal spermatozoa were $21.5{\pm}0.7\;cm$, $43.5 {\pm}5.4%$, $83.5{\pm}6.7$ million and $88.3{\pm}4.1%$, respectively. The percentages of spermatozoa with head shape and acrosome abnormalities were lower ($2.7{\pm}1.1$ and $1.4{\pm}1.3$, respectively), whereas higher percentages of abnormalities ($7.0{\pm}1.8$) were observed in mid piece and tail portion. The proportion of live spermatozoa was $28.5{\pm}5.4$. It is concluded that although a good number of morphologically normal spermatozoa are present in the insemination dose, the proportion of live spermatozoa is low, which warrants further improvements of buck semen freezing procedures to ensure good quality at AI.

• Animal Bioscience
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• 제34권2호
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• pp.198-204
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• 2021

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination. Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12). Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.181-186
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• 2020

본 연구에서는 동결정액을 활용한 농가에서 사육중인 교잡종 염소의 인공수정 효율을 조사하고자 실시하였다. 교잡종 염소들의 인공수정을 위하여 전기자극을 주어 정액을 채취한 후 동결정액을 조제하고 정자농도, 정자활력, 융해 후 정자의 활력을 조사하였다. 그 결과 교잡종 염소 수컷의 개통별 정액채취량은 2.5~3 ml 내외였고 정액의 농도는 21~25 × 10⁸/ml로 나타났다. 동결된 정액을 융해 하였을 때 정자의 활력도 90% 이상으로 높게 나타났다. 동결정액을 활용하여 교잡종 염소 사육 3 농가에서 인공수정을 실시한 후 임신진단 키트(IDEXX Rapid Visual Pregnancy Test kit)로 임신유무를 조기에 진단한 결과 각 농가별 인공수정 후 수태률은 A농장 45%, B농장 50%, C농장 68%로 조사되었다. 또한 인공수정 후 약 150일 후 출산한 교잡종 염소들의 번식성적을 조사한 결과 C농장이 68%로 분만율이 가장 높게 조사 되었고 A농장이 45%로 가장 낮게 조사되었다. 일반적으로 인공수정 후 수태율과 분만율은 약간의 차이가 나타나는 경향이 있는데 본 실험에서는 인공수정 후 임신을 조기에 진단한 결과와 분만된 개체의 분만율이 동일하게 조사되었다. 본 연구의 결과 동결정액을 활용한 교잡종 염소의 인공수정시 농가별 효율의 차이는 있으나 인공수정 효율이 45~68% 범위로 조사되었다. 본 연구결과는 일반 염소 사육 농가뿐만 아니라 축산연구기관에서 염소의 개량과 번식을 위한 기초 자료로 활용 될 수 있을 것이다.

• 한국가금학회지
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• 제45권4호
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• pp.237-244
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• 2018

본 연구에서는 닭 동결 정액의 융해 방법에 따라 정자 운동성의 변화도를 분석하였고, 적절한 융해방법에 대한 자료를 확보하였다. 동결정액을 융해하는 방법은 축종에 따라 서로 다른 열전달 효율을 제시하기도 하나, 닭 동결 정액에 대한 연구는 미진한 상태이다. 특히 닭 정액은 고 농도의 정액을 필요하기 때문에 이러한 요인에 대한 정자 운동성은 변이가 많은 것으로 알려져 있다. 그러므로, 닭 정액 융해에 필요한 온도는 $5^{\circ}C$임을 알 수 있었으며, 닭 농장에서 동결정액의 융해를 실시할 때, 알코올이 함유된 냉각수를 이용하게 되면 냉각수의 과냉각 생태를 방지할 수 있음을 관찰하였다. 또한, 동결정액을 이용한 인공수정을 실시할 때, 현장에서 직접 닭 정액을 융해하여 시간을 절약할 수 있으며, 인공 수정 작업시간이 30분을 넘기게 되면 정자의 운동성은 감소하여 수정율에 영향을 미치는 것으로 판단된다. 그러므로, 농가에서 동결정액을 활용할 경우, 융해에 신경을 써야 하며 정확한 방법을 적용하여야 수정란의 부화율 감소현상을 막을 수 있을 것으로 보인다. 이와 같이 본 연구에서 제시된 방법으로 동결정액을 이용할 때, 동결정액의 수정율이나 부화율의 변이를 막을 수 있고, 동결 정액을 활용한 가금종축생산 효율이 높아질 것으로 판단된다.