• Genomics & Informatics
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• v.21 no.1
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• pp.7.1-7.11
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• 2023

The gram-positive bacterium Listeria monocytogenes is an important foodborne intracellular pathogen that is widespread in the environment. The functions of hypothetical proteins (HP) from various pathogenic bacteria have been successfully annotated using a variety of bioinformatics strategies. In this study, a HP Imo0888 (NP_464414.1) from the Listeria monocytogenes EGD-e strain was annotated using several bioinformatics tools. Various techniques, including CELLO, PSORTb, and SOSUIGramN, identified the candidate protein as cytoplasmic. Domain and motif analysis revealed that the target protein is a PemK/MazF-like toxin protein of the type II toxin-antitoxin system (TAS) which was consistent with BLASTp analysis. Through secondary structure analysis, we found the random coil to be the most frequent. The Alpha Fold 2 Protein Structure Prediction Database was used to determine the three-dimensional (3D) structure of the HP using the template structure of a type II TAS PemK/MazF family toxin protein (DB ID_AFDB: A0A4B9HQB9) with 99.1% sequence identity. Various quality evaluation tools, such as PROCHECK, ERRAT, Verify 3D, and QMEAN were used to validate the 3D structure. Following the YASARA energy minimization method, the target protein's 3D structure became more stable. The active site of the developed 3D structure was determined by the CASTp server. Most pathogens that harbor TAS create a crucial risk to human health. Our aim to annotate the HP Imo088 found in Listeria could offer a chance to understand bacterial pathogenicity and identify a number of potential targets for drug development.

• International Journal of Aeronautical and Space Sciences
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• v.15 no.4
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• pp.383-395
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• 2014

According to the requirement of wing weight estimation and frequent adjustments during aircraft conceptual design, a wing weight estimation method considering the constraints of structural strength and stiffness is proposed to help designers make wing weight estimations rapidly and accurately. This method implements weight predictions on the basis of structure weight optimization with stiffness constraints and strength constraints, which include achievement of wing shape parametric modeling, rapid structure layout, finite element (FE) model automated generation, load calculation, structure analysis, weight optimization, and weight computed based on modeling. A software tool is developed with this wing weight estimation method. This software can realize the whole process of wing weight estimation with the method and the workload of wing weight estimation is reduced because much of the work can be completed by the software. Finally, an example is given to illustrate that this weight estimation method is effective.

• Journal of the Korean association of regional geographers
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• v.8 no.2
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• pp.247-255
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• 2002

In recent decades, the characteristics of natural hazard has been changed and the global cost of natural disasters has increased substantially. There are several trends in society and nature that suggest this pattern may continue, with more frequent mega-disasters occurring in the future. In particular, risk perception that is at odds with the "real" risk underlies the process of risk transference increases long-term vulnerability. Therefore investigating the correct risk transference and adjustments according to regional socio-economic structure can mitigate and reduce environmental, so called man-made hazard.

• Journal of Korean Public Health Nursing
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• v.31 no.1
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• pp.5-18
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• 2017

Purpose: This study was conducted to suggest a method for financial projection of health insurance expenditures that reflects future changes in demographic structure. Methods: Using data associated with the number of patients and health insurance cost per patient, generalized linear models (GLM) were fitted with demographic explanatory variables. Models were constructed separately for individual medical departments, types of medical service, and types of public health insurance. Goodness-of-fit of most of the applied GLM models was quite satisfactory. By combining estimates of frequency and severity from the constructed models and results of the population projection, total annual health insurance expenditures were projected through year 2060. Results: Expenditures for medical departments associated with diseases that are more frequent in elderly peoples are expected to increase steeply, leading to considerable increases in overall health insurance expenditures. The suggested method can contribute to improvement of the accuracy of financial projection. Conclusion: The overall demands for medical service, medical personnel, and relevant facilities in the future are expected to increase as the proportion of elderly people increases. Application of a more reasonable estimation method reflecting changes in demographic structure will help develop health policies relevant to above mentioned resources.

• BMB Reports
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• v.33 no.3
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• pp.268-275
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• 2000

In contrast to the pyrimidine (6-4)pyrimidone photoproduct [(6-4) adduct], its Dewar valence isomer (Dewar product) is low mutagenic and produces a broad range of mutations with a 42 % replicating error frequency. In order to determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar·lesion DNA decamer duplex, which contains a mismatched base pair between the 3'-T residue and an opposed G residue. The 3'-T of the Dewar lesion forms stable hydrogen bonds with the opposite G residue. The helical bending and unwinding angles of the DW/GA duplex, however, are much higher than those of the DW/AA duplex. The stable hydrogen bonding of the G 15 residue does not increase the thermal stability of the overall helix. It also does not restore the distorted backbone conformation of the DNA helix that is caused by the forming of a Dewar lesion. These structural features implicate that no thermal stability, or conformational benefits of G over A opposite the 3'-T of the Dewar lesion, facilitate the preferential incorporation of an A. This is in accordance with the A rule during translesion replication and leads to the low frequent $3'-T{\rightarrow}C$ mutation at this site.

• Structural Engineering and Mechanics
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• v.67 no.1
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• pp.1-8
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• 2018

Shake table tests performed on five 1:3 reduced scale two story RC moment resisting frames having construction defects, have shown severe joint damageability in deficient RC frames, resulting in joint panels' cover spalling and core concrete crushing. Haunch retrofitting technique was adopted herein to upgrade the seismic resistance of the deficient RC frames. Additional four deficient RC frames were built and retrofitted with steel haunch; both axially stiffer and deformable with energy dissipation, fixed to the beam-column connections to reduce shear demand on joint panels. The as-built and retrofitted frames' seismic response parameters are calculated and compared to evaluate the viability of haunch retrofitting technique. The haunch retrofitting technique increased the lateral stiffness and strength of the structure, resulting in the increase of structure's overstrength. The retrofitting increased response modification factor R by 60% to 100%. Further, the input excitation PGA was correlated with the lateral roof displacement to derive structure response curve that have shown significant resistance of retrofitted models against input excitations. The technique can significantly enhance the seismic performance of deficient RC frames, particularly against the frequent and rare earthquake events, hence, promising for seismic risk mitigation.

• The KIPS Transactions:PartD
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• v.13D no.2 s.105
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• pp.147-156
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• 2006

Event logging plays increasingly an important role in system and network management, and syslog is a de-facto standard for logging system events. However, due to the semi-structured features of Common Log Format data most studies on log analysis focus on the frequent patterns. The extensible Markup Language can provide a nice representation scheme for structure and search of formatted data found in syslog messages. However, previous XML-formatted schemes and applications for system logging are not suitable for semantic approach such as ranking based search or similarity measurement for log data. In this paper, based on ranked keyword search techniques over XML document, we propose an XML tree structure through a new data modeling approach for syslog data. Finally, we show suitability of proposed structure for semantic retrieval.

• Speech Sciences
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• v.10 no.4
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• pp.41-55
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• 2003

This paper investigates the tonal patterns of English information structure composed of topic and focus. It has been argued in previous theories that there is a significant relationship between English topic-focus structure and intonation. The English topic is marked with L+H* pitch accent and focus is marked with H* pitch accent. These theories, however, are oversimplified ones since they do not consider the contextual differences of topic and focus. To examine more concrete tonal patterns of English topic and focus, we classified topic into two subcategories of reminding topic and old-information topic. Focus was categorized into three: information focus, contrastive focus, and reference focus. The overall results show that native English speakers are inclined to use both the L+H* and H* pitch accent for the topic and focus of an utterance. We also observe a tendency to deaccentuate the topics given as old information and to mark the topics given as noun phrase with H* pitch accent. As for the intonation of focus, H* pitch accent is the most frequent type of accent, but L+H* also shows a high percentage of implementation especially in the context of correction or contrast.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.783-785
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• 2003

In recent years, the use of video in GIS is considered to be an important subject and many related studies result in VideoGIS. The virtual navigation is an important function that can be applied to various VideoGIS applications. For virtual navigation by video, the following problems must be solved. 1) Because the video route may be not exactly coincided with route that user wants to navigate, parts of several video clips may be required for single navigation. Virtual navigation should allow the user to move from one video to another at the proper position. We suggest the video segmentation method based on geographic data combined with video. 2) From a point to a destination, the change frequency of video must be minimized. The frequent change of video make user to mislead navigation route and cause the wasteful use of computing resource. We suggest methods that structure video segments and calculate weight value of each node and link.

• Geomechanics and Engineering
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• v.34 no.5
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• pp.529-545
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• 2023

The coupled soil-pile-structure seismic response is recently in the spotlight of researchers because of its extensive applications in the different fields of engineering such as bridges, offshore platforms, wind turbines, and buildings. In this paper, a simple analytical model is developed to evaluate the dynamic performance of seismically isolated bridges considering triple interactions of soil, piles, and bridges simultaneously. Novel expressions are proposed to present the dynamic behavior of pile groups in inhomogeneous soils with various shear modulus along with depth. Both cohesive and cohesionless soil deposits can be simulated by this analytical model with a generalized function of varied shear modulus along the soil depth belonging to an inhomogeneous stratum. The methodology is discussed in detail and validated by rigorous dynamic solution of 3D continuum modeling, and time history analysis of centrifuge tests. The proposed analytical model accuracy is guaranteed by the acceptable agreement between the experimental/numerical and analytical results. A comparison of the proposed linear model results with nonlinear centrifuge tests showed that during moderate (frequent) earthquakes the relative differences in responses of the superstructure and the pile cap can be ignored. However, during strong excitations, the response calculated in the linear time history analysis is always lower than the real conditions with the nonlinear behavior of the soil-pile-bridge system. The current simple and efficient method provides the accuracy and the least computational costs in comparison to the full three-dimensional analyses.