• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1527-1533
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• 2001

Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

• Journal of Conservation Science
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• v.27 no.4
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• pp.381-393
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• 2011

This study focus on the surface deterioration diagnosis based on the material characteristics of Taean Dongmunri Maaesamjonbulipsang(rock-carved standing triad Buddha Statue) and the weathering environment analysis. Rock materials of the triad Buddha is coarse-grained biotite granite which is composed mainly of quartz, K-feldspar, plagioclase, biotite and muscovite. The triad Buddha was serious surface irregularity(13.5%), exfoliation(12.3%) and discoloration(46.6%), and the physical characteristics using ultrasonic velocity appeared fourth grade (0.59, highly weathered). Korean style wooden shelter of the triad Buddha(closed type) maintained high humidity environment (daily mean; 86.6%) than exterior environment, and appeared the possibility of deterioration by freezing-thawing because the temperature showed below zero temperatures in winter. The shelter was changed from closed-type to open-type to relieve the moisture problem recently. But the moisture problem is yet to be solved because surface water flowing along northern wayside is flowed into triad Buddha. Therefore, environmental control will need to stop inflow of water into triad Buddha.

• Journal of Conservation Science
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• v.25 no.2
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• pp.179-196
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• 2009

The Gaetaesajiseokbulibsang (Treasure No. 219) consists of light gray and coarse to medium-grained granodiorite with feldspar phenocrysts in part. Magnetic susceptibility of the rock material was measured as 12.06(${\times}10^{-3}$ SI unit), being different from the granite($0.19{\times}10^{-3}$ SI unit) in the Mt. Cheonho. This indicates the raw material has been supplied from the outside. As a result of deterioration assessment, exfoliation of the Right Buddha, cement and dust of the Main Buddha were estimated as 35.2%, 21.1% and 25.0%. The ultrasonic velocity was measured as 2850.2m/s(Main Buddha), 2648.4m/s(Left Buddha) and 2644.5m/s(Right Buddha). The compressive strength calculated from the velocity showed low in the Right Buddha among three and the all pedestal parts which corresponds to the result of deterioration assessment. The indoor mean temperature and relative humidity of the shelter were $13.7^{\circ}C$ and 79.0%. It is evaluated that the indoor microclimate was stable and the shelter functioned to reduce climatic fluctuation of the outdoor. However, water condensation was occurred on the surface of the pedestal part during spring and summer, and freezing in winter season might also be done. These factors were probable to be a main cause of the surface exfoliation of the Triad Buddha Statues. Therefore, dehumidification and heating system in the shelter should be applied to prevent further deterioration.

• Economic and Environmental Geology
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• v.42 no.3
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• pp.261-272
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• 2009

An experimental study of the accelerated weathering was performed to investigate the variations of physicomechanical properties of deteriorated rocks due to freeze-thaw weathering for the Jurassic granite specimens from Wonju, Gangwon-do. Each complete cycle of freeze and thaw was lasted 24 hours, comprising 2 hours saturating in vacuum chamber, 8 hours freezing at -20$\pm1^{\circ}C$ and 14 hours thawing at room temperature. Freeze-thaw cycles were implemented with measuring the index physical properties as well as geometries of microfractures. The seismic velocity was found to decrease with increasing freeze-thaw cycles. On the other hand, absorption tends to increase with freeze-thaw cycles. In the end, it was concluded that variations of the index properties of deteriorated specimen depend on its initial properties and flaws in rock. The size and density of the traces of the microfracture on slab specimen were changed continuously with increasing freeze-thaw weathering. The results obtained in this study show that the box fractal dimension($D_B$) has the strong capability of quantifying the combined effect of size and density of the microfractures.

• Tunnel and Underground Space
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• v.19 no.2
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• pp.146-157
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• 2009

An experimental study of accelerated weathering on mudstone sample specimens from Haman, Gyeongnam was performed to investigate the variations of physico-mechanical properties of deteriorated rocks due to freeze-thaw weathering. Each complete cycle of freeze and thaw lasted 24 hours, comprising 2 hours of saturating in vacuum chamber, 8 hours of freezing at $-16{\pm}1^{\circ}C$ and 14 hours of thawing at room temperature. Total of 55 cycles of freeze-thaw were completed with measuring the index properties as well as geometries of microfractures. The measured specific gravity and P-wave velocity found to decrease with increasing freeze-thaw cycles. On the other hand, absorption ratio and effective porosity were continuously increased with increasing freeze-thaw cycles. It was found that the index properties of deteriorated sample specimen depend on its initial properties and flaws in rock. The size and density of the traces of the microfracture on slab specimen exhibited abrupt changes after 30 cycles of freeze-thaw weathering. The results obtained in this study show that the box fractal dimension($D_B$) given in this paper has the strong capability of quantifying the size and density of the microfracture.

• Food Science of Animal Resources
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• v.33 no.3
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• pp.390-394
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• 2013

The aim of this study was to determine the optimal storage condition of pre-packed Hanwoo beef without freezing. Hanwoo loin was purchased from a local distributor at 48 h after slaughter, then sliced in $1.5{\pm}0.5$ cm thickness, and packed in a polyethylene (PE) tray covered with linear low-density polyethylene (LLDPE) film. The studied factors to set the optimal storage condition were chamber temperature (5, 2.5 and $-1^{\circ}C$ for 14 d), cooling method (direct and indirect cooling system), and ultraviolet (UV) light irradiation for beef surface sterilization (0, 30, 60, and 120 min). The changes of pH, thiobarbituric acid reactive substances (TBARS) and number of aerobic bacteria were measured during storage. Beef samples stored in $-1^{\circ}C$ showed the minimal increasing rate in TBARS and microbial growth. After 15 d of storage, there was no significant difference in pH and TBARS values. However, the microbial population of beef stored in direct type cooling chamber ($4.25{\pm}0.66$ Log CFU/g) was significantly lower than that of beef stored in indirect type chamber ($6.47{\pm}0.08$ Log CFU/g) (p<0.05). After 4 d of storage, 60 or 120 min UV light irradiated beef samples showed significantly lower microbial population, and at 14 d of storage, 60 min UV irradiated beef sample showed significantly lower microbial population ($3.14{\pm}0.43$ Log CFU/g) than control ($4.46{\pm}0.13$ Log CFU/g) (p<0.05). However, TBARS values of 60 or 120 min UV light irradiated beef samples were significantly higher than non-irradiated beef sample after 4 d of storage (p<0.05).

• Research in Plant Disease
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• v.22 no.3
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• pp.198-201
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• 2016

Recently, severe dieback of apple tree has occurred in the apple orchards of Chungbuk province. Dieback rate and its casual agents have been investigated on the Chungbuk province apple orchards in 2013-2015. Out of 29,265 apple trees in the 27 orchards throughout Chungbuk province, 4,000 apple trees (13.7%) showed dieback symptoms. The causes of dieback were Phytophthora rot (50.4%), violet root rot by Helicobasidium sp. (27.1%), rodents (10%), white root rot by Rosellinia sp. (6.3%), and freezing injury (6.3%). Compared to previous reports published in 1995 and 2006, Phytophthora rot was the most dominant disease, which is thought to be due to high temperature during growing season and the increase of lowland cultivation. Results of this study will be useful to establish of the management strategy of apple tree dieback that has been increased recently.

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.339-346
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• 1994

An understanding of the structure and function of mammalian spermatozoa requires the iso-lation of these components. In this study, frozen-thawed bovine spermatozoa were treated by physical treatments (vortexing, 26 gauge needle, strained 26 gauge needles and freezing-thawing) or chemical treatments (trypsin, dithiothreitol, sodium dodecylsulfate and $\beta$-mercaptoethanoJ) to yield free heads and tails. The most effective treatment was repeated pumping of sperm suspension through a strained 26 gauge needle conneted to a syringe. Spermatozoa by this treatment were mainly broken at the junction of the head and the tail, resulting in 90-100% yields. Also, sperm head surface did not modify during strained 26 gauge needle treatment when either spermatozoa or sperm heads were incubated in 250${\mu}\textrm{g}$/ml of FITC-UEA 1 for 1 h at room temperature to detect the modification of sperm surface components. Other physical treatments were less efficient for the breakdown of spermatozoa. The effects of chemical treatments on bovine spermatozoa are not noticeable. Dissected sperm heads and tails should be fractional leading to nearly pure components by sucrose gradient centrifugation at 1,000 rpm for 15 min. The result suggest that the established method may be useful for the biochemical study of spermatozoal components, and the understanding of oocyte activation mechanism either by spermatozoal components during fertilization or microinjection of isolated components.

• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.257-262
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• 2009

This study was to investigate pregnancy rate of IVM/IVF/IVC Korean cattle (registered in government) embryos according to transport time course. For the production of embryos, oocytes recovered from slaughtered excellent grade cow and highly motile frozen-thawed bull semen (purchased from LIMC, KPN#497) was used. In vitro produced embryos were cultured in CR1aa medium for 8 days and some of them were frozen. The rate of average cleavage (>2-cell) was 83.0% (308/371) and blastocyst rate at day 8 was 34.7% (107/308). Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. These embryos were produced in a laboratory, embryo transfer (ET) was planned in 10 areas of the remote island (Jeju) from the laboratory by airplane. Thus, we examined the pregnancy rate in recipient cow according to embryo of transport time course before ET. From embryo transferred 44 recipient cows, overall pregnancy was 40.9% (18/44), these 18 cows were all calved [single, 94% (17/18); twin, 6% (1/18)] and total embryo implantation rate was 26% (19/66). Comparing transport time in the base of 6 hr, pregnancy rate in ET group required less 4 hr (60%, 9/15) was significantly higher than that required more 6 hr (26.3%, 5/19). In direct ET of freezing embryos, the pregnancy rate was 40% (4/10). However, it was difficult to find the meaning of temperature, pH and corpus luteum quality of recipients on comparison of pregnancy rate. When the cell death level of embryos according to storage time in thermos (straw container) before ET was measured by TUNEL staining, apoptotic index was increased with storage time-dependent. These results demonstrated that long distance transfer of IVM/IVF/IVC embryos is possible and the time of embryo transport is very important for the pregnancy rate on field trial.

• Horticultural Science & Technology
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• v.16 no.4
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• pp.517-519
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• 1998

This experiment was carried out to find out the effects of heat conservation materials (burying in soil, lagging, lagging +straw, nonwoven fabric, nonwoven fabric+straw) on freezing damage, labor saving, and crown gall occurrence of 'Kyoho' grapes. Temperature differences in burying in soil and lagging with $2.8^{\circ}C$ and $6.4^{\circ}C$, respectively and were considered favorable for over-wintering of grapevines. Heat conservation index in lagging +straw and burying in soil calculated from degree-hours below $-10^{\circ}C$ was 5 to 7 times higher than that of open field. Budbreak started earlier in lagging with+straw and nonwoven fabric+straw covering, and percent budbreak was increased by 22% and 7%, respectively, as well as higher than burying in soil. Diameter of bearing mother branch and length of internode and daughter branch were gross or long with soil and lagging straw and nonwoven fabric+straw. Cane growth was enhanced by burying in soil and lagging with+straw treatment. Crown gall occured higher in soil covered grape vines Labor saving was obtained in lagging with as much as 44% compared to burying in soil.