• Title/Summary/Keyword: Freezing of water

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Studies on the Ultrarapid Freezing of In vitro Fertilized Bovine Embryos I. Studies on the Survival Rates after Slow and Ultrarapid Frozen -Thawing of In Vitro Fertilized Bovine Embryos (소 체외수정란의 초급속동결에 관한 연구 I. 소 체외수정란의 완만 및 초급속동결 융해후의 생존성에 관한 연구)

  • 김상근;이봉구;이규승
    • Korean Journal of Animal Reproduction
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    • v.15 no.2
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    • pp.133-139
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    • 1991
  • This stduy was carried out in order to investigate the effects of concentration and equilibration time of cryoprotective agents on survival rate of slow and ultrarapidly frozen in vitro fertilized bovine embryos. In vitro fertilized bovine embryos, following dehydration by cryoprotective agents and sucrose, were slowly freezed(from 2$0^{\circ}C$ to -7$^{\circ}C$/-1$^{\circ}C$/min., from -7$^{\circ}C$ -35$^{\circ}C$/-0.2$^{\circ}C$/min. from -35$^{\circ}C$ to -38$^{\circ}C$/-0.3$^{\circ}C$/min.) by cell freezer and directly plunged into liquid nitrogen and thawed in 38$^{\circ}C$ water. Survival rate was defined by development rate to the morula and blastocyst stage after in vitro cultured and FDA test. The results are summarized as follows : 1. The survival rates of in vitro fertilized bovine embryos after slow frozen-thawing in the freezing medium of 0.25M sucrose added 2.5M glycerol, 3.0M DMSO, 2.0M propanediol and 2.5M glycerol+2.0M propanediol were 84.3%, 85.9%, 77.8%, 74.3%, respectively. 2. The survival rates of in vitro fertilized bovine embryos after slow frozen-thawing in the freezing of 0.50M sucrose added 2.5M glycerol, 3.0M DMSO, 2.0M propanediol and 2.5M glycerol+2.0M propanediol were 83.8%, 85.1%, 71.4%, 74.6%, respectively. 3. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing of 0.25M sucrose added 2.5M glycerol, 3.0M DMSO, 2.0M propanediol and 2.5M glycerol+3.0M propanediol were 69.3%, 70.8%, 63.2%, 67.1%, respectively. 4. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing of 0.25M sucrose added 2.5M glycerol, 3.0M DMSO, 2.0M propanediol and 2.5M glycerol+2.0M propanediol were 69.4%, 70.1%, 62.3%, 63.5%, respectively. 5. The survival rates of in vitro fertilized bovine embryos after slow and ultrarapid fromthawing in the freezing medium of sucrose added cryoprotective agents were not significant difference between 5min. and 10min. of equilibration time.

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An Experimental Study on Resistance of rapid Freezing and Thawing of Chloride-inhibiting Low-Heat Cement (차염성 저발열시멘트의 급속동결융해 저항성에 관한 실험적 연구)

  • Sim, Jong-Sung;Park, Cheol-Woo;Park, Sung-Jae;Kang, Tae-Sung;Ju, Min-Kwan;Kim, Tae-Soo
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.589-592
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    • 2008
  • This study was conducted to assess the durability of Chloride-inhibiting Low-Heat Cement while being subjected to freezing-thawing during winter seasons. Although durability varies slightly depending on the conditions of the jobsite, frost damage to concrete resulting from repeated freezing and thawing over the course of seasonal changes is the leading cause behind lowered concrete durability. in addition, concrete that has been subjected to freezing and thawing during the winter season develops a significant amount of expansive force at the core and begins to exhibit signs of damage, such as cracking, peeling, and detachment from the aggregate. Therefore, this study fabricated test specimens using a Chloride-inhibiting Low-Heat Cement(CLC) and the widely used blast furnace slag cement(BFS) and Ordinary Portland Cement(OPC) with water-to-cement ratios of 35%, 40% and 45%, respectively, to assess the durability index of the CLC as per resistance to freezing-thawing. The specimens were then tested using the KS F 2456 method (Testing method for resistance of concrete to rapid freezing and thawing) to measure the dynamic modulus of elasticity. The dynamic modulus of elasticity measurements were then used to derive the durability indices. By comparing the durability indices, it was confirmed that CLC, BFS, and OPC all had superior durability.

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The study of developing the freezing seal isolation method for the pre insulated heat transfer pipe (이중보온 열수송관에 대한 동결차수공법개발에 관한 연구)

  • You, Byounghee;Ahn, Changkoo;Kim, Woocheol;Shin, Ikho
    • Journal of Energy Engineering
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    • v.26 no.3
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    • pp.105-112
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    • 2017
  • A lot of piping systems have been used from nuclear power systems to water supply systems. The maintenance of the piping systems is needed to ensure proper operation of the piping systems. Failure of the large pipe systems especially such as KDHC(Korea District Heating Corporation) can be a matter directly related to the enterprise productivity and profitability. It can also lead to very important issues in promoting public safety and convenience. Therefore a method of quick and safety repairs have been emerged as the most important problem. In this study, freezing seal isolation method using liquid nitrogen cryogenic refrigerant was developed for the maintenance of a pre insulated heat transport pipe of KDHC with a diameter of 300 mm. In this study, by employing computational analysis techniques we performed the flow and heat transfer analysis for the targeted pre insulated heat transfer pipe and freezing seal jacket(ice-Plug) and have selected for optimal system. The detailed design model based on the results of the computational analysis finally was produced. A laboratory-scale test apparatus were designed and the freezing seal isolation self-test carried out. Also the performance assessment tests in the test bed of KDHC were carried out for on-site application.

Freeze Protection for Passive Solar Water Heating System (자연순환형 태양열온수기 동파방지기술)

  • Kim, Jong-Hyun;Hong, Hi-Ki;Chung, Jae-Dong
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.23 no.5
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    • pp.327-333
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    • 2011
  • In the present work, a new freeze protection method has been proposed for a natural circulation system of solar water heater. Though electrothermal wire is popularly used for the purpose, there are freezing troubles by wire cut-off and shortage of excessive electric power consumption. In the experimental device, hot water in storage tank was used to heat the outlet pipe from the tank if the pipe surface temperature falls lower than a set point. The cold water pipe to the storage tank was installed to directly contact the hot water pipe surface temperature rose by transferred heat.

Evaluation of Applicability of penetrating-type Nano-Coat for Preventing Deterioration of Concrete (침투형 Nano-Coat를 이용한 콘크리트 열화 방지 적용성 평가)

  • Lee, Jun Hee;Kim, Jo Soon;Sim, Yang Mo;Lee, Seung Woo
    • International Journal of Highway Engineering
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    • v.19 no.2
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    • pp.7-15
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    • 2017
  • PURPOSES : Infiltration of moisture, polluted material, and deicer into concrete, accompanied by freeze and thaw can cause significant deterioration of concrete pavement. In order to protect concrete from deterioration, it is necessary to prevent the infiltration of these concrete external materials. The moisture-repellent agent, which is a surface treatment and maintenance material added to concrete structures to render them water resistant, has advantages such as prevention of water infiltration and security against air permeation. Nano-coat, which is referred to as silicon hydride, is typically used as a moisture-repellent agent. Therefore, in this study, an attempt is made to use penetration-type Nano-coat as an alternative in order to evaluate its applicability through environmental resistance tests. METHODS : This study aimed to evaluate the applicability of penetration-type Nano-coat, which can provide water repellency to concrete, in concrete pavements, through various environmental resistance tests such as freezing and thawing resistance, chloride ion penetration resistance, and surface scaling resistance tests. The applicability of penetration-type Nano-coat was demonstrated based on the specification of KS F 2711, KS F 2456, and ASTM C 672. RESULTS :In the case of penetration-type Nano-coat applied on sound concrete, an increase in concrete durability was demonstrated by the negligible chloride ion penetrability and the absence of scaling, as revealed by visual observation of the surface, after 50 cycles of scaling resistance test. In addition, test result of the application of penetration-type Nano-coat on deteriorated concrete established that concrete surface pretreated by grinding provided improved durability than non-treated concrete. CONCLUSIONS :This study indicates that penetration-type Nano-coat is applicable as an effective alternative, to increase the durability of concrete structures. In addition, it was known that pretreatment of deteriorated concrete surface, such as grinding, is required to improve the long-term performance of concrete pavement.

Resistance to Freezing and Thawing of Concrete Subjected to Carbonation (탄산화를 받은 콘크리트의 동결융해 저항성)

  • Lee, Seung-Tae;Park, Kwang-Pil
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.623-631
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    • 2018
  • In this study, the degree of deterioration of concrete was investigated in the laboratory under conditions of carbonation and freeze-thaw cycling, which are the major causes of the deterioration of its performance. In this test, the carbonated concrete was subjected to combined freeze-thaw deterioration tests for up to 300 cycles, and its dynamic elastic modulus and compressive strength were measured. The evaluation of the effect of the water-binder ratio on normal concrete subjected to combined carbonization and freezing-thawing showed that its resistibility against such combined deterioration decreased more rapidly in the concrete with a water-binder ratio of 55 % compared with that having a water-binder ratio of 35 %. In the case where the concrete was blended with a mineral admixture consisting of fly ash and blast furnace slag at the same water-binder ratio, it showed an increase of its resistibility against combined deterioration.

Comparison of the Effects of Straight and Twisted Heat Trace Installations Based on Three-dimensional Unsteady Heat Transfer (열선의 직선시공과 감기시공의 동파방지 효과 비교를 위한 3차원 비정상 수치해석)

  • Choi, Myoung-Young;Jeon, Byoung-Jin;Choi, Hyoung-Gwon
    • Fire Science and Engineering
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    • v.30 no.1
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    • pp.49-56
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    • 2016
  • This paper numerically examines, straight and twisted electrical heat trace installations for their anti-freezing effects on water inside a pipe. The unsteady incompressible Navier-Stokes equations coupled with an energy equation were solved to compare the two installation methods. The heat conduction of the pipe with a heat source interacts with the natural convection of the water, and the conjugate heat transfer was considered using a commercial code (ANSYS-FLUENT) based on a SIMPLE-type algorithm. Numerical experiments, were done to investigate the isotherms and the vector fields in the water region to extract the evolutions of the minimum and maximum temperatures of the water inside the pipe. There was no substantial difference in the anti-freezing effects between the straight and twisted. Therefore, the straight installation is recommended after considering the damage and short circuit behavior of the electrical heat trace.

Analysis on Adequacy of the Satellite Soil Moisture Data (AMSR2, ASCAT, and ESACCI) in Korean Peninsula: With Classification of Freezing and Melting Periods (인공위성 기반 토양 수분 자료들(AMSR2, ASCAT, and ESACCI)의 한반도 적절성 분석: 동결과 융해 기간을 구분하여)

  • Baik, Jongjin;Cho, Seongkeun;Lee, Seulchan;Choi, Minha
    • Korean Journal of Remote Sensing
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    • v.35 no.5_1
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    • pp.625-636
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    • 2019
  • Soil moisture is a representative factor that plays a key role in hydrological cycle. It is involved in the interaction between atmosphere and land surface, and is used in fields such as agriculture and water resources. Advanced Microwave Scanning Radiometer 2 (AMSR2), Advanced SCATterometer (ASCAT), and European Space Agency Climate Change Initiative (ESACCI) data were used to analyze the applicability and uncertainty of satellite soil moisture product in the Korean peninsula. Cumulative distribution function (CDF) matching and triple collocation (TC) analysis were carried out to investigate uncertainty and correction of satellite soil moisture data. Comparisons of pre-calibration satellite soil moisture data with the Automated Agriculture Observing System (AAOS) indicated that ESACCI and ASCAT data reflect the trend of AAOS well. On the other hand, AMSR2 satellite data showed overestimated values during the freezing period. Correction of satellite soil moisture data using CDF matching improved the error and correlation compared to those before correction. Finally, uncertainty analysis of soil moisture was carried out using TC method. Clearly, the uncertainty of the satellite soil moisture, corrected by CDF matching, was diminished in both freezing and thawing periods. Overall, it is expected that using ASCAT and ESACCI rather than AMSR2 soil moisture data will give more accurate soil moisture information when correction is performed on the Korean peninsula.

Study on Ice Making Behavior of Water Solution with Surfactant (계면활성제 첨가수용액의 제빙에 관한 기초연구)

  • ;Hideo Inaba;Akihiko horibe
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.13 no.12
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    • pp.1175-1183
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    • 2001
  • Recently, a great attention has been paid to the ice thermal storage system for the purpose of energy saving and reduction in peak electrical demand. In the present study, it has been investigated the freezing behavior of several kinds of water solutions with nonionic surfactant. In order to prevent ice blockage in a cooled pipe, the amount and wall adhesion behavior of ice of the test fluids were observed experimentally under different concentration of water solution with surfactant, temperature of cooled wall, and the shear velocity of test fluids. The results showed that the size of ice crystal became smaller at higher shear velocity at wall. And the lowest limit of wall adhesion of ice in water solution with surfactant was found at 230 W/$m^2$ of heat flux.

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Numerical Study on the Production of Methane Hydrate by Depressurization Method (감압법을 이용한 메탄하이드레이트 생산에 대한 수치적 연구)

  • Kim, Jin-Hong;Chun, Won-Gee;Kim, Nam-Jin
    • 한국신재생에너지학회:학술대회논문집
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    • 2007.06a
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    • pp.519-523
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    • 2007
  • Gas(or methane) hydrates are solid solutions when water molecules are linked through hydrogen bonding and create host lattice cavities that can enclose a large variety of guest gas molecules. The natural gas hydrate crystal may exist at low temperature above the normal freezing point of water and high pressure greater than about 30 bars. A lot of quantities of natural gas hydrates exists in the earth and many production schemes are being studied. In the present investigation, depressurization method was considered to predict the production of gas and the simulation of the two phase flow - gas and water - in porous media is being carried out. The simulation show about the fluid flow in porous media have a variety of applications in industry. Results provide the appearance of gas and water production, the pressure profile, the saturation of gas/ water/ hydrates profiles and the location of the pressure front.

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