• International Journal of Industrial Entomology and Biomaterials
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• v.40 no.1
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• pp.22-27
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• 2020

Silkworm powder was prepared from 3^rd day of 5^th instar silkworm through various drying technology including freeze drying, hot wind drying, infrared drying, and microwave drying. The shape of silkworm dried was different with the drying methods. Freeze drying and microwave drying silkworm looked its original form, but hot wind drying and infrared drying silkworm looked shriveled and crumpled. The color of silkworm powder freeze-dried changed from yellowish green to hazel with lowering freezing temperature. Heavy metals including Pb, Cd, As, and Hg were lower the food criteria. The results of 1-deoxynojirimycin analysis was shown that BaekOkJam and GoldenSilk silkworm powder was satisfied the criteria of functional food, but YeonNokJam silkworm powder was lower than the criteria. The amino acid composition of silkworm was similar regardless of the frozen temperature in freeze drying process.

• Journal of the Korea Concrete Institute
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• v.19 no.6
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• pp.701-706
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• 2007

This study evaluated the durability of high-performance shotcrete mixed in the proper proportions using alkali-free and cement mineral accelerators as a permanent support that maintains its strength for the long term. Durability tests were performed the chloride permeability, repeated freezing and thawing, accelerated carbonation, and the effects of salt environments. Test results showed that all the shotcrete mixes included silica fume had low permeability. In addition, after 300 freeze/thaw cycles, the shotcrete mix had excellent freeze/thaw resistance more than the 85% relative dynamic modulus of elasticity. The accelerated carbonation test results were no effect of accelerator type but, the depth of carbonation was greater in the shotcrete mix containing silica fume. No damage was seen in a salt environments. Therefore, the high performance shotcrete mix proportions used in this study showed excellent durability.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.1
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• pp.173-178
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• 1993

The most appropriate mixing ratio of fly ash to bottom ash obtained from compaction characteristics and CBR values is varying from 5:5 to 6:4. But these mixed ashes are frost-susceptible materials according to judging by the paticle-size distribution because of a lot of silty-size paticles. In this study, the frost-susceptibility of compacted coal ash with proper mixing ratio is examined experimentally for use of subgrade materials. And, the efforts have been made to find proper cement addition in making these mixed ashes frost-insusceptible. It was revealed that these mixed ashes are frost-susceptible, and 4% of cement content is required to be made frost-insusceptable. It was found that amount of frost heave of these mixed ashes decreases with the unconfined compressive strength, and increases with log k if the permeability coefficient k is as low as the water into the freezing front of these mixed ashes is restricted.

• Ocean and Polar Research
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• v.33 no.2
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• pp.159-169
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• 2011

Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

• Journal of the Korea Concrete Institute
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• v.17 no.5 s.89
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• pp.743-750
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• 2005

High performance concrete containing silica fume for use in bridge deck overlay emerged as a viable type of bridge deck overlay that economic advantage in construction. They have gained acceptance in Europe, America and Canada in a relatively short time due to their low cost. In this study, high-performance concretes containing silica fume were tested and evaluated in the laboratory to assess their applicability for use in bridge deck overlay. It was conducted with experiments of mechanical and durability characteristics in compressive strength, flexural strength, chloride permeability, abrasion resistance, repeated freezing and thawing cycles and deicing salt scaling resistance. Laboratory test result describe that high-performance concrete containing silica fume for bridge deck overlay application shows most outstanding capacity.

• Ocean and Polar Research
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• v.29 no.3
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• pp.263-271
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• 2007

The survival strategies of polar organisms at permanently or extremely cold temperatures and their application to cryobiology were reviewed here. In addition, ongoing studies on psychrophiles also were described. Psychrophiles are extremophiles that can grow and reproduce in cold temperatures, typically at -10 to $20^{\circ}C$. These organisms developed various mechanisms of adaptation to extremely cold environments. Polar organisms cope with these extreme physicochemical conditions using strategies such as avoidance, protection and partnership with other organisms. Understanding on the strategies adopted by polar organisms may provide insight on the physiological process that cells can go through during freezing. Cryopreservation may be able to take advantage of the findings described above. Currently, genomes of many cold-loving organisms have been sequenced and comparative genomics has revealed, at a molecular level, the characteristics of these organisms. The investigation of microorganisms on the polar glaciers may expand our understanding on the origin of life on Earth and other planets.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.3
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• pp.201-205
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• 2015

This paper describes thermal flow characteristic in various pipelines: straight pipeline and curved pipeline. In the Arctic and ocean area, pipelines are exposed to a extremely low temperature ($0{\sim}-40^{\circ}C$). In this situation, three-dimensional flow analysis should be analyzed to investigate thermal effects such as pressure drop, temperature change, velocity deficit and distribution change of liquid droplet of internal fluid. Also, due to freezing of water droplet, impingement erosion is expected in the curved pipeline. The stability of the pipelines can be influenced by impingement erosion. In this paper, multi-phase and multi-species analysis was introduced to analyze the flow characteristics and impingement erosion of Arctic and ocean pipelines.

• Magazine of the Korean Society of Agricultural Engineers
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• v.26 no.3
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• pp.68-80
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• 1984

This study was attempted in order to investigate the effects of superplasticizers on fresh and hardened concrete. The experimental program included tests on the slump and slump loss, bleeding, time of set, air content, the compacting, factor Vee Dee, compressive strength, tensile and flexural strength, permeability, shrinkage and freege-thaw durability. The major conclusions that can be drawn on the study are as follows. 1. Superplasticizers were observed to have an appreciable fluidifying action in fresh concrete so that tinder appropriate conditions, they either considerably improved its workability or permitted a water reduction of at least 8-12% to be made while maintaining normal workability. 2. The bleeding ratios of base and S,P. Concrete were much lower than those of the conventional concrete. Differences between the base and S.P. Concrete were insignifician. 3. The setting time was the longest for conventionla concrete, followed by S.P. concrete and base concrete in thatorder. And AE water reduction admixtures showed an appreciable influence on the setting and hardening characteristics of concrete and prolonged the stiffening times. 4. The high initial slump values of S.P. concrete generally decreased rapidly with increased standing time. CF values showed increasing tendencies with the increase of S.P. content, and excessive addition of S.P. caused the segregation of fresh concrete, resulting in its rejection. 5. Though there was a slight increase in strength, no significant differences are observed between base and S.P. concrete in terms of the compressive, tensile and flexural strength. 6. The permeability of S.P. concrete was significantly less than that of the conventional concrete, and the shrinkage of S.P. concrete was considerably smaller than that of the conventional concrete, but there were no significant differences between base and S.P. concrete. 7. Compared to base concrete, S.P. concrete without entrained air tended to slightly increase freeze-thaw durability, and S.P. concrete with an appropriate entrained air gave satisfactory resistance to freezing and thawing.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.17-17
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• 2003

The milk of transgenic animals may provide an attractive vehicle for large-scale production of hEPO. Since glycosylation is cell type specific, recombinant human EPO (rhEPO) produced in different host cells contain different patterns of oligosaccharides, which could affect the biological functions. However, there have been no reports on the characteristics of rhEPO derived from milk of transgenic animals. To address this objective, several transgenic mice by using pWAPhEPO and/or pBC1hEPO expression vector were produced. However, 2 lines of pWAPhEPO founder female mouse died during late gestational day (day 18) before offspring could be obtained. They showed a severe splenomegaly, Unlike those of pWAPhEPO, mammary gland epithelial cells from biopsies of lactating pBC1hEPO transgenic mice had marked immunoreactivity to EPO and any activity was not detected in other tissues. The expression level of rhEPO is about 0.7% of mammary gland cellular total soluble proteins and an amount of 300~500 mg/L rhEPO is secreted into milk. Furthermore, the pBC1hEPO transgenic mice transmitted this character to their progeny in mendelian manner. In order to determine the extent of glycosylation variation, N-linked oligosaccharide structures present in the milk-derived rhEPO were characterized. Most of milk-derived rhEPO is fully glycosylated. the biological activity of milk-derived rhEPO was comparable to that of purified CHO-derived rhEPO, and milk-derived rhEPO showed relatively stable after freezing and thawing. Taken together, the results illustrate the potential of transgenic animals in the large-scale production of biopharmaceuticals.

• Magazine of the Korean Society of Agricultural Engineers
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• v.24 no.2
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• pp.67-75
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• 1982

A study on the effect of water reducing agent on the various characteristics of concrete has been conducted. The experimental results of the study are summarized as follows. 1. Slump test for the concrete added water reducing setretarding agent in proper quantity have been conducted. According to the test results, the decreasing rate of slump value become bigger than plain concrete with increase of the unit weight of cement and elapse of time 2. In case the proper quantity content of maximum compressive strength in Fig. 5 of water reducing set retarding agent is added, unit weight of water is decreased about 15% or so as compared with plain concrete. with the increase of water reducing set accelerating agent content unit weight of water is decreased much more, And other hand, amount of air entraining shows the increasing tendency with the increase of water reducing agent content. 3. The adding rate of water reducing agent which produce maximum strength shows that WR-CH and WR-SA which is water reducing set-starding agent is 0.2% and WR-CO is 0.5% and that WS-PO which is water reducing set accelerating agent is 0.5 4. compressive strength jof the concrete made of sulfate resistant cement shows less than the strength of normal portland cement at initial strength but the strength of both cement shows almost same at curing age of 28 days. 5. when proper quantity of water reducing set retarding agent is used, boned strength is increased about 15% at curing age of 28days. 6. According to the result of durability test, dynamic young's mudulus of elasticity at plain concrete is decreased about 50% as compared with initial step at 300 cycle of freezing and thawing after curing age of days. on the contarary the concrete used water reducing agent is decreased less than 7%.