• Title/Summary/Keyword: Freeze/Thaw

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UPTAKE OF α-AMINOISOBUTYRIC ACID (AlB) BY ROOSTER SPERMATOZOA

  • Fujihara, N.;Koga, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.2
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    • pp.91-96
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    • 1990
  • This experiment was designed to determine whether ${\alpha}$-aminoisobutyric acid (AIB) can be used to predict membrane function of spermatozoa by measuring the uptake of AIB by fresh, stored and frozen-thawed rooster spermatozoa. When spermatozoa were stored at low temperature ($0{\sim}3^{\circ}C$) for 24 h. no difference was found in AIB uptake compared with fresh spermatozoa, whereas storage for 48 h resulted in a slight increase in AIB uptake by spermatozoa. On the one hand, the uptake of AIB by frozen-thawed spermatozoa was less than that by fresh spermatozoa. This suggests possibility of a different membrane transport system between spermatozoa preserved at low temperature ($0{\sim}3^{\circ}C$) and those frozen-thawed. Glycerol used as cryoprotectant may modify rooster sperm membrane in a different manner from cold preservation. Ouabaine ($10^{-4}M$) caused a slight decrease in AIB uptake, but caffeine ($10^{-2}M$) did not influence spermatozoal AIB uptake. These results indicate a successful application of AIB to rooster spermatozoa as a mean for measuring sperm membrane function and suggest a possible alteration of membrane transport system in rooster spermatozoa between cold ($0{\sim}3^{\circ}C$) and cryopreservation ($-196^{\circ}C$).

Studies on Semisolid Infant Foods (III)-Rheological Properties of the Products- (반고체 이유보충식에 관한 연구 (III)-생물학적 조사-)

  • 이영춘
    • Journal of Nutrition and Health
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    • v.19 no.1
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    • pp.3-15
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    • 1986
  • This study was intended to investigate the rheological properties of the development formula of infant foods and the results are summarized as follow ; 1) Consistency of starch solutions measured by Brabender Anylograph and Brookfield viscometer showed that waxy rice and riece with malt had lower consistency and more rheological stability.The flow type of tested raw materials and formula was found to ? pseudoplastic, as judged by n-value of 0.332 -0.692, and no yield value. 2) The influenced of temperature on consistency could be accounted for by the equation, In (n) = K(1/T) + const. this relationship indicated that consistency of tested sample increased as temperature decreased. The consistency of waxy rice, rice with malt and formula A were less affected by the temperature change. 3) Infant foods with malt(formula A ) ad with waxy rice maintained better freeze-thaw stability in terms of consistency and starch aggregation indicating that this infant food could be stored for a long term in the freezer section of the home refrigeratory without adverse effect on the product quality. 4) From the above experiments, it would necessarily follow that infant food can be easily made at home the food-stuffs generally available around us, and that the easiest and safest way to store them lies in making them into semisolid state, and in keeping them in frozen state.

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On the Spatial and Temporal Variability of L-band Polarimetric SAR Observations of Permafrost Environment in Central Yakutia

  • Park, Sang-Eun
    • Korean Journal of Remote Sensing
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    • v.33 no.1
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    • pp.47-60
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    • 2017
  • The permafrost active layer plays an important role in permafrost dynamics. Ecological patterns, processes, and water and ice contents in the active layer are spatially and temporally complex depending on landscape heterogeneity and local-scale variations in hydrological processes. Although there has been emerging interest in the application of optical remote sensing techniques to permafrost environments, optical sensors are significantly limited in accessing information on near surface geo-cryological conditions. The primary objective of this study was to investigate capability of L-band SAR data for monitoring spatio-temporal variability of permafrost ecosystems and underlying soil conditions. This study exploits information from different polarimetric SAR observables in relation to permafrost environmental conditions. Experimental results show that each polarimetric radar observable conveys different information on permafrost environments. In the case of the dual-pol mode, the radar observables consist of two backscattering powers and one correlation coefficient between polarimetric channels. Among them, the dual-pol scattering powers are highly sensitive to freeze/thaw transition and can discriminate grasslands or ponds in thermokarst area from other permafrost ecosystems. However, it is difficult to identify the ground conditions with dual-pol observables. Additional backscattering powers and correlation coefficients obtained from quad-pol mode help understanding seasonal variations ofradar scattering and assessing geo-cryological information on soil layers. In particular, co-pol coherences atHV-basis and circular-basis were found to be very usefultools for mapping and monitoring near surface soil properties.

The Moisture Migration of Compacted Clay Liners in the Landfill on Winter Condition (겨울철 조건하의 폐기물매립지 점토층의 수분이동)

  • 이재영;최상일
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1997.05a
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    • pp.47-52
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    • 1997
  • The experimental investigations considered in this paper are similar in many respects to those of Lee$^1$, with some key differences. First, there is no layering of the soils in a heterogeneous liner. The only soil investigated is the clay component of the cover liner. This ensures that the clay is exposed to freezing and that frost propagation in the clay can be investigated separate from other processes. Second, a closed system approach to the simulation was adopted. According to Jones$^2$, closed-system freezing occurs when there is no source of water available beyond that originally present in the soil voids. Freezing under such conditions results in very thin or non-existent ice lenses. One of tile objectives of the experiments described in this paper was the moisture migration and the changing of moisture contents of the compacted clay liner in landfill. The closed-system was used to limit tile variables in the experimental simulation to make these calculations more direct, although the final results could be applied to an open system also. As a result, the moisture content decreased about 45%-46% after two freeze/thaw cycles.

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A Study on the Estimation of Adhesive Stability of the Improved Direct Setting Method using Tile Bond for Application of Porcelain Tile under 1 percent absorptance (흡수율이 1% 이하인 자기질 타일의 타일접착제를 사용한 벽체 개량 떠붙임 공법의 부착안정성 평가 연구)

  • Jung, Yang-Hee;Jung, Eun-Hye;Seo, Sin-Seok;Kim, Ook-Jong;Lee, Do-Bum
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2008.11a
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    • pp.13-16
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    • 2008
  • This paper is to present the performance data for improved direct setting method using tile bond for application of porcelain tile under 1 percent absorptance. For this purpose, improved direct setting method type 1 & 2(tile bond curing time 0, 24H) were compared with the conventional setting methods(including direct setting method and improved pressure setting method) in the sight of the adhesive stability of porcelain tile. It tested for tiles after 14, 28days under standard condition and severe conditions. The severe conditions were water immersion, heat ageing(70℃) and freeze-thaw cycle. On the basis of test results, the adhesive strength of direct setting method was lowest for the conventional setting methods. But improved direct setting method using tile bonds(A, B) came close to the result of improved pressure setting method using tile bonds.

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An Experimental Study on the Freeze-Thaw Resistance of Concrete Incorporating Waste Foundry Sand (폐주물사를 혼입한 콘크리트의 동결-융해 저항성에 관한 실험적 연구)

  • Yong, Suk-Ung;Lee, Joo-Hyung;Hong, Chang-Woo;Yun, Kyong-Ku;Park, Je-Seon
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.04a
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    • pp.117-122
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    • 1998
  • Concrete structures has been deteriorated by the freezing and thawing due to temperature gap. This study was conducted to evaluate durabilite of concrete which are increasingly demanded recently. Therefore the research of durability must be executed for application of waste foundry sand concrete real structures. Concrete durability must be executed for application of waste foundry sand concrete real structures. Concrete durability properties incorporating waste foundry sand was performed with the variable of W/C ratio, Sand/Waste foundry sand ratio and Air entrainment-Non air entrainment. Cylinder specimens were made and subjected to freezing and thawing cycle at $-18^{\cire}C$ and $4^{\cire}C$. Dynamic modulus of elasticity were evaluated as F/T cycle increase. The results show that strength of concrete is increased the W/C ratio decrease, the Sand/Waste foundry sand ratio increase when the concrete contains AE agent and decreasing WC ratio and AE concrete makes improved resistance of freezing and thawing improved. Especially, resistance of freezing and thawing is improved by Fine aggregate/Waste foundry sand ratio which is 50%, 25%, 0% in a row. Therefore it is turn out the waste foundry sand could be applied to concrete from the experiment.

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Moisture Sensitivity and Aging Effects of Recycled Wastepaper fiber Cement Composites (폐지섬유보강 시멘트 복합체의 수분민감성 및 열화특성)

  • 원종필;배동인;박찬기;박종영
    • Journal of the Korea Concrete Institute
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    • v.14 no.2
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    • pp.148-155
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    • 2002
  • This research investigates the moisture and aging effects of wastepaper fiber-cement composites. Wastepaper fibers is obtained by a dry process. Wastepaper fiber-cement composites was manufactured by the hatscheck process. The effects of moisture and aging on the performance of wastepaper fiber-cement composites were investigated through accelerated laboratory tests simulating the effects of moisture sensitivity and wet-dry cycles as well as freeze-thaw cycles and long-term drying. They were shown to possess acceptable moisture and aging performance compared with virgin fiber cement composites.

A Study on Cooking Method of Millet Nochi (기장 노치 제조 방법에 관한 연구)

  • 김희정;이혜수
    • Korean journal of food and cookery science
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    • v.7 no.2
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    • pp.75-80
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    • 1991
  • Millet Nochi is a kind of Korean traditional food which is not well known to the general public. This study was crried out in order to elucidate the cooking method and investigate the changes of textural properties during retrogradation. Cooking method for millet Nochi were as follows. 1. Millet is soaked in water for 3 hours, mashed, sifted by 20 mesh 3 screen. 2. The millet flour is mixed with malt (amount of malt is 0.025 times of millet flour by weight) 3. This mixture is steamed for 20 minutes. 4. Steamed mixture is again mixed with the same amount (0.025 times of millet by weight) of malt. 5. The mixture is incubated at $60^{\circ}C$ for 2 hours. 6. After incubation, the mixture is panfried. 7. The changes of textural properties during 30 freeze-thaw cycles, except adhesiveness were not significantly appeared.

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Investigation of steel fiber effects on concrete abrasion resistance

  • Mansouri, Iman;Shahheidari, Farzaneh Sadat;Hashemi, Seyyed Mohammad Ali;Farzampour, Alireza
    • Advances in concrete construction
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    • v.9 no.4
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    • pp.367-374
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    • 2020
  • Concrete surfaces, industrial floors, sidewalks, roads and parking lots are typically subjected to abrasions. Many studies indicated that the abrasion resistance is directly related to the ultimate strength of the cured concrete. Chemical reactions, freeze-thaw cycles, and damages under abrasion are among many factors negatively affecting the concrete strength and durability. One of the major solutions to address the abrasive resistance of the concrete is to use fibers. Fibers are used in the concrete mix to improve the mechanical properties, strength and limit the crack propagations. In this study, implementation of the steel fibers in concrete to enhance the abrasive resistance of the concrete is investigated in details. The abrasive resistance of the concrete with and without steel fibers is studied with the sandblasting technique. For this purpose, different concrete samples are made with various hooked steel fiber ratios and investigated with the sandblasting method for two different strike angles. In total, 144 ASTM verified cube samples are investigated and it is shown that those samples with the highest steel fiber ratios have the highest abrasive resistance. In addition, the experiments determine that there is a meaningful correlation between the steel fiber percentage in the mix, strike angle and curing time which could be considered for improving structural behavior of the fiber-reinforced concrete.

Direct Extraction of DNA from Soil for Amplification of 16S rRNA Gene Sequences by Polymerase Chain Reaction

  • Cho, Jae-Chang;Lee, Dong-Hun;Cheol, Cho-Young;Cho, Jang-Cheon;Kim, Sang-Jong
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.229-235
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    • 1996
  • Microgram quantities of DNA per gram soil were recovered with SDS- based and freeze-and thaw procedures. The average DNA fragment size was > 23 Kb. This method generated minimal shearing of extracted DNA. However, the DNA extracts still contained considerable amounts of humic impurities sufficient to inhibit PCR. Several approaches were used to reduce the interferences with the PCR (use of CTAF in extraction step, Elutip-d column purification, addition of BSA to PCR buffer) to accomplish PCR with DNA extract as a template. Most of the DNA extracts were not digested completely by restriction endonuclease, and CTAB-TREATED ane Elutip-d column purified DNA extracts were partially digested. Regarding as restriction enzyme digestion, all PCRs failed to amplify 16S rRNA gene fragments in the DNA extracts. In the case of DNA extracts only where BSA was added to PCR buffer, PCR was successfully conducted whether the DNA extracts were treated with CTAB or purified with columns. However, these two treatments were indispensable for humic impurity-rich DNA extracts to generate the PCR-compatible DNA samples. Direct extraction of DNA, coupled with these procedures to remove and relieve interferences by humic impurities and followed by the PCR, can be rapid and simple method for molecular microbiological study on soil microorganisms.

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