• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.126-126
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• 2014

Label-free biomolecular assay based localized surface plasmon resonance (LSPR) of noble metal nanoparticles enables simple and rapid detection with the use of simple equipment. Nanosized metal nanoparticles exhibit a strong absorption band when the incident light frequency is resonant with the collective oscillation of the electrons, which is known as the LSPR. Here we demonstrate localized surface plasmon resonance (LSPR) substrates such as plasmonic Au nanodisks fabricated by a nanoimprinting process and gold nanorod-immobilized surfaces and their applications to highly sensitive and/or label-free biosensing. To increase detection sensitivity various bioreceptors weree designed. A single chain variable fragment (scFv) was used as a receptor to bind C-reactive protein (CRP). The results of this effort showed that CRP in human serum could be quantitatively detected lower than 1 ng/ml. Aptamers, which were immobilized on gold nanorods, were used to detect mycotoxins. The specific binding of ochratoxin A (OTA) to the aptamer was monitored by the longitudinal wavelength shift of LSPR peak in the UV-Vis spectra resulting from the changes of local refractive index near the GNR surface induced by accumulation of OTA and G-quadruplex structure formation of the aptamer. According to our results, OTA could be quantitatively detected lower than 1 nM level. Additionally, aptamer-functionalized GNR substrate was quite robust and can be regenerated many times by rinsing at 70 OC to remove bound target. During seven times of washing steps, the developed OTA sensing system could be reusable. Moreover, the proposed biosensor exhibited selectivity over other mycotoxins with an excellent recovery for detection in grinded corn samples, suggesting that the proposed LSPR based aptasensor plays an important role in label-free detection of mycotoxins.

• 대한조선학회논문집
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• 제29권4호
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• pp.114-131
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• 1992

본 논문은 2차원 자유표면파문제에서 시간영역해법을 이용하여 2차원 운동문제에 적용할 수 있는 수치해석을 하였다. 경계조건으로는 엄밀한 물체표면 경계조건과 비선형 자유표면경계 조건을 부과했다. 수치해를 구하는데는 코시이론을 이용하여 제2종 프레드흘름 경계적분방정식을 도출하고 이를 이산화시켜 처리하였다. 수치계산을 위해 전영역을 유한한 영역으로 제한하여야 한다. 제한된 영역에서 방사해의 부과를 위해 전영역을 수치해석영역과 외부영역으로 나누고, 외부영역의 해는 그린 제2정리를 이용하고, 선형자유표면조건을 만족하는 과도그린함수를 사용한다. 위의 그린 제2정리를 이용한 식으로 부터 초기조건, 선형 자유표면조건, 무한원방조건을 이용하여 단순화시킨 다음 포텐셜과 유동함수의 관계식으로 치환하면 비선형해와 정합할 수 있는 정합행렬을 구할 수 있다. 본 논문에서 개발한 정합방법을 이용하여 적용할 문제로서 첫째는 주상체가 상하동요, 수평동요하는 경우 계산이고 두번째로는 수면하에서 타원형실린더가 일정속도로 항진할 때 계산을 수행한 결과를 고차스펙트럴방법과 비교하였다.

• The Plant Pathology Journal
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• 제33권5호
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• pp.441-449
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• 2017

Citrus canker is an important bacterial disease of citrus in several regions of the world. Strains of Xanthomonas citri type-A (Xc-A) group are the primary pathogen where citrus canker occurs. After Xc-A entered the Northeast of Argentina in 1974, the disease spread rapidly from 1977 to 1980 and then slowed down and remained moving at slow pace until 1990 when it became endemic. Citrus canker was detected in Northwest Argentina in 2002. This paper presents the main steps in the fight of the disease and the management strategies that have been used to control citrus canker at this time. We think the process might be usefull to other countries with the same situation. Results from more than 40 years of research in Northeast (NE) Argentina indicate that we are at the limit of favorable environment for the disease. The severity of citrus canker is greatly affected by the environment and El $Ni{\tilde{n}}o$ Southern Oscillation (ENSO) phenomenon which causes cyclic fluctuations on the disease intensity in the NE region. Weather-based logistic regression models adjusted to quantify disease levels in field conditions showed that the environmental effect was strongly modulated by the distance from a windbreak. Production of healthy fruits in citrus canker endemic areas is possible knowing the dynamics of the disease. A voluntary Integrated Plan to Reduce the Risk of Canker has been in place since 1994 and it allows growers to export unsymptomatic, uninfested fresh fruit to countries which are free of the disease and require healthy, pathogen free fruits. The experience from Argentina can be replicated in other countries after appropriate trials.

• 세라미스트
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• 제22권3호
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• pp.281-300
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• 2019

Surface enhanced Raman scattering (SERS) was first discovered in 1974 by an unexpected Raman signal increase from Pyridine adsorbed on rough Ag electrode surfaces by the M. Fleishmann group. M. Moskovits group suggested that this phenomenon could be caused by surface plasmon resonance (SPR), which is a collective oscillation of free electrons at the surface of metal nanostructures by an external light source. After about 40 years, the SERS study has attracted great attention as a biomolecule analysis technology, and more than 2500 new papers and 500 review papers related to SERS topic have been published each year in recently. The advantages of biomaterials analysis using SERS are as follows; ① Molecular level analysis is possible based on unique fingerprint information of biomolecule, ② There is no photo-bleaching effect of the Raman reporters, allowing long-term monitoring of biomaterials compared to fluorescence microscopy, ③ SERS peak bandwidth is approximately 10 to 100 times narrower than fluorescence emission from organic phosphor or quantum dot, resulting in higher analysis accuracy, ④ Single excitation wavelength allows analysis of various biomaterials, ⑤ By utilizing near-infrared (NIR) SERS-activated nanostructures and NIR excitation lasers, auto-fluorescence noise in the visible wavelength range can be avoided from in vivo experiment and light damage in living cells can be minimized compared to visible lasers, ⑥ The weak Raman signal of the water molecule makes it easy to analyze biomaterials in aqueous solutions. For this reason, SERS is attracting attention as a next-generation non-invasive medical diagnostic device as well as substance analysis. In this review, the principles of SERS and various biomaterial analysis principles using SERS analysis will be introduced through recent research papers.

• 한국전자파학회논문지
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• 제22권5호
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• pp.522-527
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• 2011

본 논문에서는 54 GHz 대역의 위상 고정 루프에서 사용되기 위한 Ring 발진기를 이용한 3 분주 주입 동기 주파수 분주기(Injection-Locked Frequency Divider: ILFD)를 0.13-${\mu}M$ Si RFCMOS 공정을 이용하여 설계, 제작한 결과를 보인다. 1.8 V의 공급 전압에 대해서 buffer단을 포함하여 70 mW의 전력을 소비하며, 입력 신호가 없을 때 0~1.8 V의 varactor 조정 전압 범위에 대하여 18.92~19.31 GHz에서 자유 발진(free-running oscillation)을 하였다. 0 dBm의 입력 전력에 대해서 1.02 GHz(54.82~55.84 GHz)의 동기 범위(locking range)를 가지며 varactor 조정(0~1.8 V)을 포함한 동작 범위(operating range)는 약 2.4 GHz(54.82~57.17 GHz)를 보였다. 제작된 회로의 크기는 측정 pad를 포함하여 0.42 mm${\times}$0.6 mm이며, pad를 제외한 실제 동작 영역의 크기는 0.099 mm${\times}$0.056 mm이다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.196-196
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• 2012

Graphene, two-dimensional one-atom-thick planar sheet of carbon atoms densely packed in a honeycomb crystal lattice, has grabbled appreciable attention due to its extraordinary mechanical, thermal, electrical, and optical properties. Based on the graphene's high carrier mobility, high frequency graphene field effect transistors have been developed. Graphene is useful for photonic components as well as for the applications in electronic devices. Graphene's unique optical properties allowed us to develop ultra wide-bandwidth optical modulator, photo-detector, and broadband polarizer. Graphene can support SPP-like surface wave because it is considered as a two-dimensional metal-like systems. The SPPs are associated with the coupling between collective oscillation of free electrons in the metal and electromagnetic waves. The charged free carriers in the graphene contribute to support the surface waves at the graphene-dielectric interface by coupling to the electromagnetic wave. In addition, graphene can control the surface waves because its charge carrier density is tunable by means of a chemical doping method, varying the Fermi level by applying gate bias voltage, and/or applying magnetic field. As an extended application of graphene in photonics, we investigated the characteristics of the graphene-based plasmonic waveguide for optical signal transmission. The graphene strips embedded in a dielectric are served as a high-frequency optical signal guiding medium. The TM polarization wave is transmitted 6 mm-long graphene waveguide with the averaged extinction ratio of 19 dB at the telecom wavelength of $1.31{\mu}m$. 2.5 Gbps data transmission was successfully accomplished with the graphene waveguide. Based on these experimental results, we concluded that the graphene-based plasmonic waveguide can be exploited further for development of next-generation integrated photonic circuits on a chip.

• 한국해안·해양공학회논문집
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• 제22권1호
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• pp.47-57
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• 2010

Woo and Liu (2004)의 확장형 Boussinesq FEM 수치모형에서 한계점으로 지적되었던 수치진동현상과 계산 효율성이 크게 개선되었다. 수치진동을 해결하기 위해 subgrid scale stabilization method를 사용하였고, 계산효율성을 높이기 위해서 Hessian 연산자를 도입하였으며, 유속벡터에 대한 행렬 구성을 하나의 행렬로 구성하였다. 또한 추가변수에 대한 행렬은 mass lumping technique을 사용하여 대각행렬로 구성하였다. Vincent and Briggs(1989)의 파랑 굴절 및 회절에 대한 수치실험 결과 수치진동현상이 확연히 줄어 들은 것을 관찰할 수 있었으며, 수리실험 결과와도 상당히 일치하는 결과를 보였다. 이전 모형에 비해 약 10배의 계산소요시간이 줄어 향후 항만부진동이나 퇴적물 이동과 같은 현실적인 문제에 적용이 가능할 것으로 기대된다.

• 한국전산유체공학회지
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• 제13권4호
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• pp.126-134
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• 2008

We developed a three dimensional Navier-Stokes code based on the level set method to simulate two phase flows with high density ratio. The Navier-Stokes equations with consideration of the surface tension effects are solved by using SIMPLE algorithm on a non-staggered grid. The present code is validated by simulating two test problems. First one is to simulate a rising bubble inside a cube. The thickness of the interface of the bubble is shown to affect the pressure distribution around the interface. As the thickness decreases, the pressure field around the interface becomes more oscillatory. As the bubble rises, a ring vortex is shown to form around the interface and the bubble eventually develops into an ellipsoidal shape. Merge of two bubbles inside a container is secondly tested to show the robustness of the present code for two phase flow simulation. Numerical results show stable and reliable behavior during the process of merging of two bubbles. The velocity and pressure fields around the interface of bubbles are shown oscillation free during the merging of two bubbles.

• Wind and Structures
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• 제14권5호
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• pp.413-434
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• 2011

Without output covariance estimation, one reference-based Stochastic Subspace Technique (SST) for extracting modal parameters and flutter derivatives of bridge deck is developed and programmed. Compared with the covariance-driven SST and the oscillation signals incurred by oncoming or signature turbulence that adopted by previous investigators, the newly-presented identification scheme is less time-consuming in computation and a more desired accuracy should be contributed to high-quality free oscillated signals excited by specific initial displacement. The reliability and identification precision of this technique are confirmed by a numerical example. For the 3-DOF sectional models of Sutong Bridge deck (streamlined) and Suramadu Bridge deck (bluff) in wind tunnel tests, with different wind velocities, the lateral bending, vertical bending, torsional frequencies and damping ratios as well as 18 flutter derivatives are extracted by using SST. The flutter derivatives of two kinds of typical decks are compared with the pseudo-steady theoretical values, and the performance of $H_1{^*}$, $H_3{^*}$, $A_1{^*}$, $A_3{^*}$ is very stable and well-matched with each other, respectively. The lateral direct flutter derivatives $P_5{^*}$, $P_6{^*}$ are comparatively more accurate than other relevant lateral components. Experimental procedure seems to be more critical than identification technique for refining the estimation precision.

• 센서학회지
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• 제26권1호
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• pp.66-72
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• 2017

The three dimensional ultrasonic anemometer was constructed to reduce the disadvantages of the two-dimensional anemometer and to be free from the use environment. Three pairs of transmitting and receiving ultrasonic sensors were designed to face each other at an angle of $45^{\circ}$ to the upper and lower surfaces at intervals of $120^{\circ}$. 200 kHz ultrasonic sensor Oscillation, transmission and reception, level detection, power supply circuit were designed and U, V, W wind speed vector components were obtained by measuring the time of first received ultrasonic pulse by transmitting pulse ultrasound. It is implemented as firmware in ARM Coretex-M3 processor so that horizontal and vertical wind direction and wind speed can be converted into digital signal by vector calculation. In this study, The three-dimensional ultrasonic anemometer can complement the disadvantages of the two-dimensional anemometer (mechanical and ultrasonic), and it is expected to gradually replace the two-dimensional anemometer due to its high utilization rate by collecting additional information such as vertical wind.