• Journal of the Korea Society for Simulation
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• v.20 no.2
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• pp.11-17
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• 2011

The purpose of this study is 3 dimensional modeling of lower part of drilling system in offshore rig and simulation of drilling process. Recently, shipbuilding companies have been focusing on offshore rigs due to their high added-value and the reduced demand of new shipbuilding. In most cases, however, the basic design, installation and management of offshore rig are carried out by foreign companies. Therefore, it is difficult to obtain the knowledge and information of drilling system. In this study drilling devices, BOP(Blowout Preventor) and cementing job and mud circulation related components are included as the main components of offshore rig. The structure and function of them were analyzed from a viewpoint of object-oriented technique. On the basis of this analysis they were modeled in the 3 dimensional structure with 3D software tool such as CATIA and 3DVIA Composer. The drilling process was simulated according to the scenario of drilling operation. This simulation system can be effectively used for an educational tool for students and engineers in ocean plant industries.

• Journal of the Korean GEO-environmental Society
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• v.17 no.8
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• pp.5-15
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• 2016

As the increase of domestic sewage treatment plant and reinforcement of the standard of effluent water quality continues, the volumes of sewage sludge are consistently increasing. Existing treatment of sewage sludge, such as incineration and ocean dumping has been prohibited because of air pollution and prohibitions towards ocean dumping, and in turn, recycling and energy recovery from waste methods have being studied recently. However, the lack of technical level and systematic analyses of application technique are problems, that future analysis of such relative technique would be required. The present study has been investigated as follows, management technique of sludge, patent on refuse-derived fuel and increase biogas production and utilizable technology, trend of research paper. Furthermore, research development from five developed nations (Korea, Japan, China, United States, and United Kingdom), domestic and foreign have been investigated and analyzed by section. In this study, the future technical field which is required for effective management of sludge has been suggested.

• Journal of Species Research
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• v.9 no.1
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• pp.26-34
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• 2020

The phylum Bacteroidetes covers phenotypically diverse groups of Gram negative rods that do not form endospores, and currently includes 6 classes, 6 orders, 33 families and 380 genera. Members of Bacteroidetes can be aerobic and anaerobic heterotrophs, hydrogen utilizing chemolithotrophs, or methylotrophs. They can be isolated from diverse habitats including terrestrial and aquatic environments, environments with extreme physicochemical conditions, and animal and plant hosts. During a series of extensive surveys of prokaryotic species diversity in Korea, bacterial strains belonging to Bacteroidetes were isolated from various sources of aquatic and terrestrial environments. A total of 22 isolates were obtained, which represent 22 unrecorded species in Korea belonging to 14 genera of 6 families. Sixteen species among them were assigned to Flavobacteriaceae, two species were to Sphingobacteriaceae, and single species was to each of the families Bacteroidaceae, Balneolaceae, Chitinophagaceae and Cytophagaceae. At genus level, Chryseobacterium (5 species) and Flavobacterium (5 species) were the most abundant genera, and single species were obtained for the genera Bacteroides, Baloneola, Terrimonas, Dyadobacter, Aquimarina, Arenibacter, Gillisia, Gilvibacter, Salinimicrobium, Winogradskyella, Pedobacter and Sphingobacterium. The detailed descriptions of each unrecorded species are provided.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.673-677
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• 1997

It has been reported that E. senticosus is effective for strengthening the liver, reduction of blood sugar levels, antistress and rainforcement of stamina. However, most of the extraction from this medicinal plant is imported from foreign countries. Therefore, farmers have tried to grow it in this country even though it is difficult to propagate by seeding method. This study was carried out to improve seeding propagation using seeds in National Honam Agricultural Experinment Station from '92 to '93. The seeds of E. senticosus were immature embryo at the time of gathering seeds in autumn, and needed wet stratification treatment for after-ripening. Embryo elongated slowly for a few days after stratification treatment, and cotyledon started to develop from 40 days after treatment. There were significantly positive correlation between after-ripening period and the ratio of embryo length. Dehiscence was the highest in the ratio of embryo length of 60∼70％, and percentage of dehiscent seed after treatment for 150 days was 76.5％.

• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.256-262
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• 2003

To enhance the production of heavy chain immunoglobulin G (HC IgG) in the suspension cultures of transgenic tobacco cells (Nicotiana tabacum), the effects of adding various cytokinins (i.e., growth regulators) and organic nitrogen sources to culture media were investigated. Four different cytokinins including kinetin, isopentenyladenine (IPA), 6-benzylaminepurine (BA), and zeatin were tested with or without dichlorophenoxyacetic acid (2,4-D), which is a typical growth regulator supplemented in the standard Murashige and Skoog (MS) medium. The productivity of intracellular HC IgG was increased by 36 and $42\%$, compared to the control, especially when IPA (2 mg/l) or BA (0.2 mg/l) was added to the media in the presence of 2,4-D, respectively. In the study of organic nitrogen sources, addition of each casein hydrolysate and tryptone to the culture media at a final concentration of 0.01 and 1 g/l, respectively. increased the productivity or he IgG as much as 68 and $67\%$, respectively, in comparison with the control, which was is MS medium without supplementation of any organic nitrogen sources. This study shows that the optimization of media composition could offer significant improvements in the production of foreign proteins in the suspension cultures of transgenic plants.

• Journal of Species Research
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• v.7 no.3
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• pp.231-239
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• 2018

While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.115-120
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• 1990

A series of experiments were carried out for two successive years to investigate the productivity of forage turnip in mid-northern area of Korea. Fresh matter and dry matter yields of 6 foreign cultivars (Purple Top Strap Leaved, Purple Top White Globed, Seven Top, Shogoin, White Egg and Amber Globe) seeded either in early spring or in autumn after harvesting forage corn were evaluated with different seeding date and harvesting date as well as with different level of fertilization. The results obtained are summarized as follows: 1. The earlier the seeding date in spring (Mar. 29, Apr. 12, Apr. 16) was, the higher was the yield of fresh matter and dry matter as the plant growth ceased after mid June, when the temperature began to rise. 2. In spite of its high DM yield, the Japanese cultivar, Shogoin, showed poor forage quality for summer feeding due to its earlier bolting in harvesting season. 3. Of the 6 cultivars Purple Top White Globed showed highest DM yield (800 kg/ 10a) in mid area including SEOUL irrespective of planting season. 4. Although the turnips showed mostly positive yield responses to fertization, the differences were not great especially above the level of$N-P_2O_5-K_2O$: 5-3-4 kg/lOa. 5. IN TAEGWALLYONG, a northern area of Korea, the average fresh matter yield of the six cultivars tested amounted to 3,500 kg/lOa when drilled on June 30 and harvested on Aug. 30, although bulb formation during the summer was relatively poor. 6. Concluded, forage turnip is regarded to be a suitable catch-crop which has the potential to maintain and increase the total forage production in mid-northern area of Korea. The intercropping is recommendable especially for autumn planting well past the time forage corn has been harvested.

• Journal of the Korean Institute of Gas
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• v.8 no.2 s.23
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• pp.48-53
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• 2004

Korea Gas Corporation(KOGAS) is a Liquified Natural Gas(LNG) supplier through out the Korea. LNG, which is imported wholly from foreign countries, is compressed 1/600 for easy transportation and is stored in a liquid state in the storage tanks at Incheon, Pyeongtaek and Tongyeong. At LNG receiving terminals, LNG is vaporized to natural gas before supplying to City Gas Consumer or Power Plant. The secondary pump is a equipment which compress LNG from $10 kgf/cm^2$ to $70 kgf/cm^2$. The secondary pump at Tongyeong LNG receiving terminal is consisted of two pumps in one underground PIT, and is connected to supporting structures. It is therefore expected that there is a vibration problem with the pump and was found that high level vibration was occurred in a low frequency band(5${\~}$10Hz). In this paper, the vibration of secondary pump was analyzed, and the main cause of vibration was found out.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.179-185
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• 2021

BACKGROUND: Tomato genome editing using CRISPR-Cas9 is being actively conducted in recent days, and lots of plant researches have been aiming to develop high valued crops by editing target genes without inserting foreign genes. Many researchers have been involved in the manipulation of the crop ripening process because fruit ripening is an important fruit phenotype for increasing fruit shelf life, taste, and texture of crops. This paper intends to evaluate target sgRNA to edit the two ripening-related genes encoding pectate lyase (PL) and phytoene synthase (Psy) with the CRISPR-Cas9 system. METHODS AND RESULTS: The CRISPR-Cas9 expression vector was cloned to target the PL (Solyc03g111690), Psy1 (Solyc03g031860), and Psy2 (Solyc02g081330) genes, which are the ripening genes of tomatoes. Tomatoes injected with Agrobacterium containing the CRISPR-Cas9 expression vector were further cultured for 5 days and used to check gene editing efficiency. As a result of the target gene sequence analysis by the next generation sequencing method, gene editing efficiency was calculated, and the efficient target location was selected for the PL and Psy genes. CONCLUSION: Therefore, this study was aimed to establish target sgRNA data that could have higher efficiency of the CRISPR-Cas9 system to obtain the delayed ripening phenotype of tomato. The developed method and sgRNA information is expected to be utilized in the development of various crops to manage its ripening processes.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.572-575
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• 2011

A new gerbera cultivar 'Whiteny' was released by the National Institute of Horticultural & Herbal Science in 2009. A cross was made between 'Wonder King' with white semi-double and 'Ever Green' with pale greenish white and semi-double in 2007. After evaluation of the characteristics for four years (from 2007 to 2010), it was selected specially for the use of cut flower. The 'Whiteny' cultivar has pure white color (RHS W155D) with black disc center and semi-double flower. The average flower diameter is 11.5 cm, large-sized flower. And inner ray floret and disc diameter are 7.8 cm and 2.2 cm, respectively. It has a long peduncle height, 52.2 cm, and longer vase life, 12.1 days. And it is also adaptable to the local cultural environment of Korea. It is expected that if the 'Whiteny' cultivar is planted, the royalty paid to foreign varieties can be saved. And the local growers will also be benefitted since the cost of cultivars will be lower than the imported ones.