• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.411-418
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• 2012

This study was conducted to develop the technique for cultivation of forage crops using swine slurry in fallow paddy land located in the mid-mountain area (FPL). The field experiments were carried out from 2007 to 2009 on FPL at Kumsan, Chungbuk province in Korea. Swine slurry was prepared which decayed for 6 months. The experimental plots were consisted of seven treatments; tall fescue-based mixed pasture applied with chemical fertilizer (Control), mono-tall fescue pasture (MTF), tall fescue-based mixed pasture (TFBM), mono-Perennial ryegrass (MPR), mono-Italian ryegrass (MIR), mono-Red clover (MRC and mono), Reed canarygrass (MRCG) applied with swine slurry. The field of tall fescue-based pasture had been sown with a grasses mixture containing 'Fawn' tall fescue, 'potomac' orchardgrass, 'Reveille' Perennial ryegrass, and 'kenblue' Kentuky bluegrass, 'Kenland' Red clover. Seeding rates were 16, 6, 4, 2 and 2 (kg) per ha, respectively. DM yields of forages and rates of grass coverage were higher in MTF, TFBM and MRCG as compared with control treatment. This result means that FPL has contained with favorable conditions for growing grass, because forage productivity is more than 14.5 tons per ha per year in fallow paddy land. In addition, the farmer can save the trouble of repeated plowing and sowing every year, with the introduction of perennial grasses. The farmer must conduct the re-seeding and induce the improvement of management methods for the elevation of the persistence of red clover and perennial ryegrass, because both red clover and perennial ryegrass having high nutritive value and palatability was less persistent. Therefore, we suggest that FPL may be the good land for forage production utilizing swine slurry and swine slurry can be applied on FPL without any negative effects on DM production and the property of soil. FPL of Korea can be better utilized by applying swine slurry to the mono and/or mixed swards.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.1-10
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• 2004

This pot experiment was conducted to investigate the effects of combined micronutrient application($T_1$;control, $T_2$; Fe, $T_3$; Fe＋Mn, $T_4$; Fe＋Mn＋Cu, $T_5$ ; Fe＋Mn＋Cu＋Zn, $T_6$ ; Fe＋Mn＋Cu＋Zn＋Mo, $T_7$ ; Fe＋Mn＋Cu＋Zn＋Mo＋B) on forage performance of pure and mixed cultures of orchardgrass and white clover. The third part was concerned with the changes in the contents and yields of N-compounds (crude/pure protein and soluble N-compounds) in forages. The results obtained are summarized as follows: 1. The contents of N-compounds(crude/pure protein and soluble N-compounds) were generally different according to the forage species, whether it was a pure or mixed culture, and additional fertilization, especially N. In orchardgrass, these contents were relatively low at the $T_3$ and $T_6$ in both pure and mixed cultures. In white clover, these contents were relatively decreased by the $T_1$, $T_3$, and $T_6$ in mixed culture. 2. The treatments influenced relatively more on the yields of crude/pure protein than on the dry matter yields of forages, and this tendency was more significant in white clover than in orchardgrass. 3. In white clover, the great differences in the yields of crude protein by the treatments occurred especially in mixed culture and at 5th cut without no additional fertilization. In white clover, the positive effects of optimum treatments on the yields of crude protein seemed to be decreased by the additional fertilization, especially N. In mixed culture, the favorable growth of white clover by the optimum treatments tended to be positively related to the favorable contents and yields of N-compounds. The changes in the yields of pure protein were similar to the tendency of crude protein

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.11-20
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• 2004

This pot experiment was conducted to investigate the effects of combined micronutrient application($T_1$;control, $T_2$; Fe, $T_3$; Fe＋Mn, $T_4$: Fe＋Mn＋Cu, $T_5$ ; Fe＋Mn＋Cu＋Zn, $T_6$ ; Fe＋Mn＋Cu＋Zn＋Mo, $T_7$ ;Fe＋Mn＋Cu＋Zn＋Mo＋B) on forage performance of pure and mixed cultures of orchardgrass and white clover. This 4th part was related to the changes in the contents of micronutrients(Fe, Mn, Cu, Zn, Mo, and B) in forages. The results obtained are summarized as follows: 1. General differences have been showed in the contents of micronutrients based on the treatments, forage species, pure/mixed culture, cutting order, and additional fertilization, especially N. Compared to pure culture, orchardgrass showed relatively high contents of Mn and Zn, and low contents of B and Fe in mixed culture. White clover, however, tended to be exactly opposed to the above trends. The contents of Cu and Mo did not show any differences between pure and mixed cultures. 2. In relative comparison, the $T_7$ influenced negatively on the contents of Cu, Zn, and Mo in orchardgrass. The $T_7$ also influenced negatively on the contents of Mo in white clover. However, the $T_7$ influenced positively on the contents of Mn in orchardgrass, and also influenced positively on the contents of Fe, Mn, and Cu in white clover. Because of the antagonism between Fe and Mn, the Fe contents in both forages were significantly decreased by the $T_3$. Under the various conditions, the differences among Fe contents tended to be more significant in white clover than in orchardgrass. 3. At the $T_6$ and $T_7$, the Mo contents in both forages tended to be relatively high. The Mo contents, however, were somewhat decreased by the $T_7$ 7/. The Mo-toxicity, which was caused by the high Mo-contents, tended to be diminished, and was likely to be prevented by the optimum B/Mo ratio and B application($T_7$ ).

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.121-128
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• 1991

Forage productivity of two sorghum hybrids, Pioneer(P) 931 and P 956 and a sorghum-sudangrass hybrid, P 988, was studied. The 1st-cut of sorghun hybrids was made for silage 6 times from heading stage at a week-interval and regrowth (2nd-cut) was harvested on Oct. 13 although plants were not reached the same growth stage of 1st-cut. However, the 1st- and 2nd-cut of sorghum-sudangrass were made for green fodder 6 times from 4 weeks before heading stage at a week-interval. 1. Percent dry matter(DM) of 1st-cut of sorghum and 1st- and 2nd-cut of sorghum-sudangrass increased as growth stage advanced. Percent DM of 2nd-cut of sorghum decreased as the 1st-cut date delayed due to insufficient growing period on Oct. 13. 2. In the 1st-cut of sorghum the proportion of leaf blade (LB) or culm+leaf sheath (LS) to total DM decreased, but that of panicle increased as growth stage advanced. The panicle proportion of P 956 increased and culm+LS decreased more rapidly compared with those of P 931. IIowever, in sorghum-sudangrass hybrid the proportion of LB decreased and culmf LS increased as growth stage advanced through heading stage. 3. In the 1st-cut of sorghum hybrids crude(C) protein and C. fiber decreased, but nitrogen free extract (NFE) increased as growth stage advanced although C. fat and C. ash were similar among the growth stages. In the 2nd-cut of sorghum C. protein and C. ash increased, but NFE decreased as harvesting date delayed due to plants were younger although C. fat and C. fiber were similar among the harvesting dates. In the sorghum-sudangrass C. protein of 1st-cut decreased, but C. fiber of 1st-cut and NFE of the 1st-and 2nd-cut increased as growth stage advanced. 4. DM yield of 1st-cut of sorghum increased from heading stage to 3 weeks after heading and then levelled off, but total DM including regrowth was similar among the harvesting time. In the sorghum-sudangrass hybrid DM yield of 1st-cut and total DM yield including 2nd-and 3rd-cut increased as the harvestingtime delayed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.193-198
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• 2006

Effects of acetosyringone and on Agrobacterium-mediated transformation of orchardgrass were investigated. Embryogenic calli induced from 3 varieties, Frontier, Potomac and Roughrider, were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II(NPTII) and intron-containing ${\beta}-glucuronidase$ (intron-GUS) genes in the T-DNA region. The effects of varieties and acetosyringone(AS) concentrations on transformation and the expression of the GUS gene were investigated. Inclusion of $200{\mu}M$ AS in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of orchardgrass.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1062-1062
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• 2001

The concept of “precision agriculture” or “site-specific farming” is usually confined to the fields of soil science, crop science and agronomy. However, because plants grow in soil, animals eat plants, and humans eat animal products, it could be argued (perhaps with some poetic licence) that the fields of feed quality, animal nutrition and animal production should also be considered in this context. NIR spectroscopy has proved over the last 20 years that it can provide a firm foundation for quality measurement across all of these fields, and with the continuing developments in instrumentation, computer capacity and software, is now a major cog in the wheel of precision agriculture. There have been a few giant leaps and a lot of small steps in the impact of NIR on the animal world. These have not been confined to the amazing advances in hardware and software, although would not have occurred without them. Rapid testing of forages, grains and mixed feeds by NIR for nutritional value to livestock is now commonplace in commercial laboratories world-wide. This would never have been possible without the pioneering work done by the USDA NIR Forage Research Network in the 1980's, following the landmark paper of Norris et al. in 1976. The advent of calibration transfer between instruments, algorithms which utilize huge databases for calibration and prediction, and the ability to directly scan whole grains and fresh forages can also be considered as major steps, if not leaps. More adventurous NIR applications have emerged in animal nutrition, with emphasis on estimating the functional properties of feeds, such as in vivo digestibility, voluntary intake, protein degradability and in vitro assays to simulate starch digestion. The potential to monitor the diets of grazing animals by using faecal NIR spectra is also now being realized. NIR measurements on animal carcasses and even live animals have also been attempted, with varying degrees of success, The use of discriminant analysis in these fields is proving a useful tool. The latest giant leap is likely to be the advent of relatively low-cost, portable and ultra-fast diode array NIR instruments, which can be used “on-site” and also be fitted to forage or grain harvesters. The fodder and livestock industries are no longer satisfied with what we once thought was revolutionary: a 2-3 day laboratory turnaround for fred quality testing. This means that the instrument needs to be taken to the samples rather than vice versa. Considerable research is underway in this area, but the challenge of calibration transfer and maintenance of instrument networks of this type remains. The animal world is currently facing its biggest challenges ever; animal welfare, alleged effects of animal products on human health, environmental and economic issues are difficult enough, but the current calamities of BSE and foot and mouth disease are “the last straw” NIR will not of course solve all these problems, but is already proving useful in some of these areas and will continue to do so.

• Journal of Animal Environmental Science
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• v.8 no.1
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• pp.35-42
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• 2002

This study was carried out to determine the effect of chemical fertilizer and two fermented types of pig slurry on the total dry matter yield and quality of sorghum $\times$ sudangrass hybrid (Sorghum bicolor L. Moench) and chemical properties of soil after harvest in Jeju area. Three treatments consisted of chemical fertilizer (CF) 200kg 7kg Nha $^1$, aerobic fermented pig slurry (PS)200kg 7kg Nha $^1$, and aerobic fermented pig slurry treated with probiotics (PS+P) 200kg 7kg Nha $^1$were arranged in a randomized block design with three replications. The results obtained are summarized as follows. The heights of plant applied fermented pig slurry were slightly taller than those of plants applied chemical fertilizer during early growing stage, but there was no difference among treatments when the plants were harvested. The total forage dry matter yields were in the range of 14,848~ 15,42kg/ha, but they were not significantly different. Also, CP, NDF, ADF and mineral contents in the forage(% of DM basis) did not differ among treatments. The pH of soil was ranged from 5.35 to 5.63, but it was not significantly different. However, the content of available $P_2O_5$ of soil was higher(P<0.05) in chemical fertilizer treatment than that of soil in fermented pig slurry treatments. The content of K was higher(p<0.05) when ffrrmented pig slurry treated with probiotics was applied after the 1st harvest. but it was not different among treatments after the and harvest.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.267-274
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• 2005

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, a thermptolerance gene, DgP23, was introduced into orchardgrass using Agrobacterium - mediated transformation method. PCR and Southern blot analyses using genomic DNA showed specific DNA band on agarose gel and hybridization signal on X- ray film in transgenic orchardgrass harboring the recombinant DgP23 gene, but not in the wild type and empty vector control plants. RT-PCR and Southern blot analyses using total RNA also showed specific DNA band and hybridization signal. Transgenic orchardgrass did not showed ny morphological aberration both in the green house and field cultivation. Thermotolerance of transgenic plants was not detected in laboratory test. but may detected in field test.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.273-280
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• 1999

Hypocotyl explants of Medicago saliva cv. Vernal were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of growth regulators. After six weeks of culture, somatic embryos were formed from calli on MS medium containing $4mg/{\ell}$ 2,4-D and $0.1mg/{\ell}$ kinetin, or $4mg/{\ell}$ 2,4-D and $0.5mg/{\ell}$ kinetin. The mature somatic embryos were developed to plantlets when subcultured on MS basal medium. In order to obtain secondary somatic embryogenic calli, cotyledon of regenerated plantlets were cultured on a callus induction medium. Embryogenic calli were formed on MS medium containing $4mg/{\ell}$ 2,4-D alone. For induction and development of secondary somatic embryogenesis, the embryogenic calli were transferred to MS basal medium containing either 2,4-D or NAA. Multi-secondary somatic embryogenesis was the most effective on MS basal medium with $0.1mg/{\ell}$ 2,4-D. The rate of secondary somatic embryo formation of regenerated plants was 18 times higher than that of seed grown plants. The mature secondary somatic embryo were germinated into plantlets on MS basal medium after six weeks of culture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.155-170
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• 2021

Oats (Avena sativa L.) represent a good forage crop for cultivation in regions with short growing periods and/or cool weather, such as the mountainous areas of southern Korea. In this study, using the Korean elite summer oat varieties 'High speed' and 'Dark horse', we aimed to determine the optimal time to plant and harvest forage oats seeded in spring and summer in a mountainous area. Seeds were planted three times from late February and early August at 9- or 10-days intervals, respectively, and plants were harvested three times from late May to October at 10-day intervals. The experiment was carried out in an upland field (Jangsu-gun Jeonbuk) in 2015 and 2016. We investigated the changes in forage yield (FY) and quality [crude protein (CP) and total digestible nutrient (TDN) contents] based on the time of planting and harvest. Neither the forage quality nor yield of either spring and summer oats was significantly influenced by the time of planting. The CP of spring oats harvested three times at 10-day intervals from late May was 12.0%, 8.2%, and 6.5%, thereby indicating a reduction with a delay in the time of harvest. In summer oats, CP ranged from 8.4% to 8.7%, although unlike CP in spring oats, was not significantly influenced by the time of harvest. For both forage types, harvest time had no significant effect on TDN. The FY of spring oats harvested in late May and early and mid-June was 10.2, 18.7, and 19.5 ton ha^-1, respectively, with that of oats harvested on the latter two dates being significantly increased by 83% and 91%, respectively, compared with that in late May. Similarly, the FY of spring oats harvested in late October and early and mid-November was 7.1, 12.5, and 12.1 ton ha^-1, respectively, with that of oats harvested on the latter two dates being significantly increased by 75% and 71%, respectively, compared with that in late October. Taking into consideration forage yield and quality (not less than 8% CP), it would be profitable to plant spring oats in the mountainous areas of southern Korea until March 15 and harvest around June 10, whereas summer oats could be beneficially planted until August 25 and harvested from early November.