• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.929-934
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• 2000

Optimum stand density of alfalfa (Medicago sativa L.) varies with locations and climates. Stand density is one of the factors that determines herbage yield, forage quality and persistence of alfalfa. As establishment costs increase, the question arises whether present population densities are optimum for obtaining maximum herbage yield and forage quality. The objectives of this study were: 1) to determine the optimum plant density for highest herbage yield and forage quality for the dehydrated alfalfa industry under Edmontons climatic conditions in Alberta, Canada; 2) to compare herbage yield and forage quality of the cultivars 'Algonquin' and 'Vernal' grown at a range of stand densities. Alfalfa seedlings of both cultivars were either transplanted at spacings of 6, 10, 15 and 25 cm or direct seeded at the 4.5 cm plant spacings, providing population densities of 494, 278, 100, 45 and $16plants/m^2$. The experimental design was a randomized complete block with a split-plot arrangement having three replicates; the main plots consisted of two alfalfa cultivars Algonquin and Vernal, and the sub-plots were the five population densities. The cultivar Vernal had significantly higher annual yield than did the cultivar Algonquin. There was no significant effect of plant density on herbage yield. There was no difference in crude protein (CP) between the two cultivars. At the first cut, there was a significant quadratic effect of plant density on CP content and the greatest CP occurred at the 100 plants/m2 density. Crude protein was not affected by plant density at the second cut. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) were not affected by plant density. The cultivar Algonquin usually had a lower ADF and NDF than cultivar Vernal. In conclusion, high population densities ($278plants/m^2$ or more) of alfalfa did not improve herbage yield and forage quality compared with low plant population densities ($100plants/m^2$ or less) of alfalfa.

• 한국초지조사료학회지
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• 제22권1호
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• pp.23-30
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• 2002

본 연구는 제주지역에서 양마의 주당 본수 1(16,000주/10a), 2(34,000주/10a), 3(48,000주y10a), 4(64,000주/10a), 및 5본(80,000주/10a)에 따른 생육특성, 사료수량성 및 사료가치를 검토하고, 가축 조사료로 이용하기 위하여 2001년 4월 13일부터 10월 8일까지 수행하였으며, 그 결과를 요약하면 다음과 같다. 초장은 주당 1본에서 176cm였으나 주당 2본에서 185cm로 길어졌으나 그 이상 주당 본수가 많아짐에 따라 점차적으로 작아져서 5본에서는 166cm로 작아졌다. 생초, 건물, 단백질 및 TDN수량은 주당 2본에서 각각 114.6MT/ha, 17.3MT/ha, 2.33MT/ha, 9.21MT/ha로 가장 증수되었으나 그 이상과 그 이하의 본수에서는 점차적으로 감소되었고, 주당 5본에서는 생초수량 44.6MT/ha, 건물 수량 4.9MT/ha 단백질 수량 0.75MT/ha, TDN 수량은 2.83MT/ha로 감수되었다. 주당 본수가 1본에서 5본으로 많아짐에 따라 조단백질 함량 13.1%에서 15.4%로, 조지방 함량 3%에서 3.7%, 가용무질소물 20.1% 에서 23.2%로, TDN 함량은 41.1%에서 45.4%로 증가되었으나 조섬유와 조회분 함량은 각각 41.2%에서 34.9%로, 9.4%에서 8.2%로 감소되었다.

• 한국초지조사료학회지
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• 제9권2호
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• pp.103-107
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• 1989

남부지방에 적합한 사초용유채의 파종적기를 구명코자 다수성 품종으로 선별된 Velox를 공시하여 실험했던 바 그 결과를 요약하면 다음과 같다. 1. 초장, 분기수, 엽수등의 수량구성 형질은 9월중 하순에 파종한 구가 가장 우수하였다. 2. 생초수량과 건물수량도 역시 9월 중하순에 파종한 구가 증수되었다. 3. 조필자질 함량은 파종기가 늦어질수록 높았고 NDF, ADF, cellulose 및 lignin 등의 조직유함량은 파종기가 늦어질수록 저하되었다. 4. IVDMD은 일정한 경향을 발견할 수 없었고 가소화건물수량은 9월 중하순에 파종한 구가 높았다.

• 한국초지조사료학회지
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• 제9권3호
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• pp.179-186
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• 1989

사초용유채의 품종선별선급실험과 유채용유채와 사초용유채품종들 간에 있어서 생산성과 영양가치를 비교한 실험에서 사초용유채 도인품종중 우리나라 남부지역의 특성에 적합한 품종으로 Velox가 사초생산성 및 영양가치면에서 가장 유망하다고 생각되었다. 따라서 Velox를 공시하여 고립적배와 실행群落培 사이에 생산성과 영양가치에 있어서 어떤 차이가 있는가를 비교하였으며 또한 생육과정중의 영양가치의 변화를 조사하였는데 그 결과를 요약하면 다음과 같다. 1. 수당 생산성에 있어서 생체중과 건물중의 개체간 변이가 매우 컸으나 고립적배가 군락적배보다 1%수준의 유의차로 우수하였다. 孤립적배에 있어서 생체중과 건물중의 구성은 분지에 크게 의존되었으며 군락적배에 있어서는 분치와 주경이 비슷한 영향을 주었다. 건물율은 고리적배와 군락적배사이에 큰 차이가 없었으며 부위별로는 주경이 가장 높았다. 2. 粗蛋白質含量은 生育期間이 經過됨에 따라 점차 저하되었으며, 播種後 90일부터 120일까지에는 孤立적배가 群落적배보다 높은 반면 180일以後 개화기까지는 군락적배가 고립적배보다 높았다. NDF, ADF, cellulose 및 lignin등의 鐵維含量은 生育초기에는 낮은 水준이었으며 생育期間이 經過됨에 따라 점차 높아지면서 생育後期에는 孤立적배가 군락적배보다 높게 變化되었다. 3. In vitro 乾物소화率은 줄기나 잎 모두 生育기간이 경과됨에 따라 점차 저하되었으며, 줄기는 군락적배가 고립적배보다 높았으나 잎은 고립적배와 군락적배간에 유의차가 인정되지 않았다.

• 한국초지조사료학회지
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• 제18권1호
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• pp.19-26
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• 1998

The effects of different fertilizer management on herbage production and botanical composition were determined. Field experiments were conducted during 1991 - 1993 on sandy loam soil at Allgiu south western Germany under variabling fertilizer management; cattle slurry, NPK, PK chemical fertilizer management and zero fertilization. The dry matter yield of forage was the highest in the plot of mineral fertilizer and lowest in the plot of without fertilization. The dry matter yield of P-K application was higher by 1.2 ton than that of without fertilization. The content of crude protein, crude fibre, digestible dry matter yield, net energy and nitrogen content of plants was not significantly different among different fertilizer management. N uptake of plants was in the order NPK chemical fertilizer (347kg N/ha) > cattle slurry (337kg N/ha) > P-K chemical fertilizer (325kg N/ha) > without fertilization (3 15kg N/ha).

• 한국초지조사료학회지
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• 제21권3호
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• pp.145-150
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• 2001

This study was conducted to obtain the transformed birdsfoot trefoil (Lotus corniculatus L.) plants with BcHSP17.6 gene using Agrobacterium turnefaciens LBA4404 and we confirmed transformed gene from the regenerated birdsfoot trefoil plants. The expression vector, pBKH4 vector, harboring BcHSP17.6 gene was used for production of transgenic birdsfoot trefoil plants. The callus of birdsfoot trefoil was cocultivated with Agrobacteriurn turnefaciens and transformed calli were selected on kanamycin-containing SH-kc medium to regenerate into plants. The transformed birdsfoot trefoil plants were produced 4 momths after cultivation on BOi2Y medium. The transgenic birdsfoot trefoil plants were analyzed by isolation of genomic DNA and genomic Southern hybridization using a -32P labelled BcHSPl7.6 fragments. (Key words : Birdsfoot trefoil, Transgenic plant. BcHSP17.6 gene, Callus induction, Plant regeneration)

• 한국초지조사료학회지
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• 제34권3호
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• pp.187-192
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• 2014

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

• 한국작물학회지
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• 제46권2호
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• pp.95-99
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• 2001

Soybean [Glycine max (L.) Merr.) is known to produce the highest total digestible mutrients (TDN) yield among summer grain legumes in Jeju area but little is known about the effects of cultural practices on forage yield and quality. A determinate soybean cv. Baegunkong was planted on 5 June, 20 June, and 3 July and grown at four plant densities (30, 50, 70 and 90 plants $m^{-2}$ in 1998 in Jeju to evaluate the effects of planting date and plant density on the yield and quality of soybean forage. Days to flowering decreased from 47 to 38 days, average plant height from 61 to 51cm and main stem diameter from 6.31 to 5.00mm as planting was delayed from 5 June to 3 July. Average plant height quadratically increased from 45 to 62cm as plant density increased from 30 to 90 plants $m^{-2}$. Planting date did not affect the average dry matter, crude protein, and TDN yields. The average dry matter and TDN yields displayed a quadratic response to plant density and the optimum plant density for both dry matter and TDN yields was estimated about 60 plants $m^{-2}$. Plant density had no effect on crude protein yield. Planting date did not significantly influence forage quality. The crude protein content was not significantly influenced by plant density. Increasing plant density slightly increased acid detergent fiber content but slightly decreased TDN content.

• 한국초지조사료학회지
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• 제20권2호
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• pp.97-102
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• 2000

To increase thennotolerance of forage crops, transgenic rice plants as a model for transformation of monocots were generated. A cDNA encoding the chloroplast-localized small heat shock protein (small HSP) of rice, Oshsp21, was introduced into rice plants via Agrobacterium-mediated gene transfer system. Calli induced from scutella were co-cultivated with a A. tumefaciens strain EHAlOl canying a plasmid, pIGhsp21. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of Oshsp2l gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot and immunoblot analyses revealed that the Oshsp21 gene was constitutively expressed and accumulated as mature protein in transgenic plants. Effects of constitutive expression of the OshspZl on thermotolerance were first probed with the chlorophyll fluorescence. Results indicate that inactivation of electron transport reactions in photosystem I1 (PSII), were mitigated by constitutive expression of the Oshsp21. These results suggest that the chloroplast small HSP plays an important role in protecting photosynthetic machinery during heat stress. (Key words : Thermotolerance, Rice, Transgenic, cDNA)

• 한국초지조사료학회지
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• 제10권3호
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• pp.147-151
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• 1990

The purpose of this study is to clarify the relation of survival rates of seedling plants under grown orchardgrass populations and control by the difference cutting frequency. The orchardgrass populations investigated in this study have passed for 10 years after establishment. Cutting frequencies were 3, 4 and 5 times in a year. This experiment was conducted from April to October, 1989 at the experimental field of Institute of Agricultural Development, Yonsei University. The results are summarized as follows: 1. The survival rates of seedling plants decreased by the days after sowing. The final survival rates of seedling plants were 7.8%, 38.9% and 33.8% in 3, 4 and 5 cuttings, respectively. 2. Relative light intensity indicated highly possitive correlation with relative survival rates of seedling plants and negative correlation with plant length of orchardgrass populations in 4 and 5 cuttings. 3. The frequency distribution of survival seedling plants at 177 days after sowing were showed high values in seeding spots near the adjacent plants of 3 cutings, but in the middle of seeding spots between adjacent plants of 4 .and 5 cuttings, respectively. 4. The plant length of seedling became longer towards the seeding spots near the adjacent plants but the number of tillers increased slightly up to the middle of seeding spots between adjacent plants in all cutting frequencies.