• 한국초지조사료학회지
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• 제17권4호
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• pp.379-386
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• 1997

대만산 배추 (Brassica campestris M.)로부터 분리한 BcHSPI7.6 cDNA(내열성 유전자)를 pBKSl-l vector에 subcloning하므로서, NPTII 유전자와 P35S-HSPI7.6 cDNA를 가지는 pBKH4 재조합 플라스미드를 제작하였다. 이들 플라스미드를 갖는 A. tumefaciens LBA4404로서 담배잎 단편을 24시간 동안 공배양하므로서 감염시켰으며, 이들 유전자로 형질전환된 shoot는 $100\;{\mu\textrm{g}}/ml$의 가나마이신을 첨가한 MS-n/B 배지에서 선발하였다. 담배((Nicotiana. tabacum)의 열에 대한 치사온도는 $50^{\circ}$에서 15분 이상이었으며, BcHSPI7.6 cDNA를 갖는 형질전환된 식물체는 이 온도에서 내열성을 나타내었다. 식울체의 형질전환 여부는 ${\alpha}^{_32}P$로 표지한 BcHSPI7.6 cDNA 단편을 probe로 이용해서 Southern blot hybridization을 실시하므로서 확인하였다. BcHSPI7.6 cDNA의 발현정도는 Northern blot 분석과 이중면역확산 방법으로 확인하였다. 본 연구에서, 담배에 도입한 BcHSPI7.6 cDNA는 내열성과 관련이 있는 유전자로서, HSPI7.6 단백질은 식물체를 열에 의한 손상으로부터 방지해 주는 protector 역할을 하는 것 으로 사료된다.

• 한국초지조사료학회지
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• 제8권2호
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• pp.128-134
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• 1988

This experiment was carried out to compare chemical composition of corn-cowpea inter cropping and corn monocropping plants at different harvestion time and obtained the following results. 1. In both cropping systems, the contents of crude fat and nitrogen free extract (NFE) were significantly increased (P<0.01), while that of crude protein crude fiber and crude ash were decreased (P<0.01) with each harvesting time. 2. The acid detergent fiber (ADF) and crude fiber contents were decreased same patterns in growing period, however, according to maturing of corn ears the difference between ADF and crude fiber contents reduced. 3. At mature stage, crude protein yields per 10a in corn monocropping and corn-cowpea intercropping system were 127.6kg and 152.1kg, respectively. The difference of crude protein content between corn-cowpea intercropping and corn monocropping system was 19.2%. 4. TDN yields of each cropping system, at mature stage, obtained similar results and TDN yields per 10a of corn monocropping and corn-cowpea intercropping system were 1006.1kg and 990.1, respectively. 5. Conseqently, corn-cowpea intercropping system could be increased protein yield without decreasing of dry matter yield in comparison with corn monocropping system.

• 한국초지조사료학회지
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• 제16권2호
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• pp.155-160
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• 1996

Spring sown oat crops were grown during early March to late June in 1993~1994. The crops were harvested at different stage of maturity to determine the yield performance, chemical components and net energy value of the plants for silage utilization. The pattern of dry matter accumulation, absolute growth rate(Abs. GR), leaf weight ratio(LWR), stalk diameter(SD) and other growth parameters were analysed to evaluate the suitability of the plant materials for ensiling. The effect5 of wilting treatment and formic acid addition on the quality of silage was also disscused during the experiments. Spring sown oat crops was evaluated as a suitable materials for whole plant silage making. The best qualitative oat silage was obtained at the stage of soti dough to hard dough, when the plant reached 32.2~35.7% in dry matter concentration. Spring sown oat produced 9.43 ton in DM basis, 5.79 ton in TDN and 57.550 MJ-NEL/ha in net energy lactation. Early harvesting of the oat corps needed wilting treatment of the material before ensiling. Under pre-witing treatment (1/2 day) and formic acid addition(0.3%) silage quality point of oat crops harvested at the bloom stage was improved from 58 to 84 point.

• 한국초지조사료학회지
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• 제10권1호
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• pp.48-54
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• 1990

In order to investigate effects of starch addition on the fermentative quality and dry matter digestibility (DMD) of Kudzu, Puerarie thunbergii Bentham plants were ensiled by the conventional method in small experimental plastic silo of 7.5 liter with different levels of starch addition. The fermentative quality, neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) of silage were determined by chemical analysis, and in vitro dry matter digestibility (DMD) by pepsin-cellulase technique. Total digestibile nutrients (TDN), digestible energy (DE) and metabolizable energy (ME) were calculated with DMD. The results obtained were as follows; 1. Acetate, butyrate, PH, NDF, ADF, and ADL of Kudzu silage were reduced with increasing of starch addition but the contents of lactate and total acid were increased. 2. The DMD was marked 47.5, 49.5, 51.2, 57.9 and 62.0 % under starch addition with a rate of 0, 2, 4, 6 and 8 %, respectively. 3. TDN, DF and ME were increased with high rate of starch addition. 4. The regression equation and correlation of Kudzu silage between level of starch (X) and DMD (Y) were Y= 41.6 + 1.86 X (I= 0.96, P < 0.01), of silage between Flieg's score (X) and DMD (Y) were Y= 41.6 + 0.23 X (I= 0.96, P < 0.01) and of silage between level of starch (X) and Flieg's score (Y) were Y= 21.3 + 7.97 X (I= 0.97, P < 0.01), respectively.

• 한국초지조사료학회지
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• 제8권3호
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• pp.128-134
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• 1988

This experiment was carried out to compare chemical composition of corn-cowpea intercropping and corn monocropping plants at different harvesting time and obtained the following results. 1. In both cropping systems, the contents of crude fat and nitrogen free extract (NFE) were significantly increased (P<0.01), while that of crude protein, crude fiber and crude ash were decreased (P<0.01) with each harvesting time. 2. The acid detergent fiber (ADF) and crude fiber contents were decreased same patterns in growing period, however, according to maturing of corn ears the difference between ADF and crude fiber contents reduced. 3. At mature stage, crude protein yields per 10a in corn monocropping and corn-cowpea intercropping system were 127.6kg and 152.lkg, respectively. The difference of crude protein content between corn-cowpea intercropping and corn monocropping system was 19.2%. 4. TDN yields of each cropping system, at mature stage, obtained similar results and TDN yields per 10a of corn monocropping and corn-cowpea intercropping system were 1006.lkg and 990.1, respectively. 5. Conseqently, corncowpea intercropping system could be increased protein yield without decreasing of dry matter yield in comparison with corn monocropping system.

• 한국초지조사료학회지
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• 제37권3호
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• pp.237-241
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• 2017

Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

• 한국초지조사료학회지
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• 제40권4호
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• pp.285-290
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• 2020

To understand the role of small heat shock protein (sHSPs) in rice plant response to various stresses such as the heat and oxidative stresses, a cDNA encoding a 24.1 kDa mitochondrial small HSP (Oshsp24.1) was isolated from rice by rapid amplification of cDNA ends (RACE) PCR. The deduced amino acid sequence shows very high similarity with other plant small HSPs. DNA gel blot analysis suggests that the rice genome contains more than one copy of Oshsp24.1. High level of expression of Oshsp24.1 transcript was observed in rice seedlings in response to heat, methyl viologen, hydrogen peroxide, ozone, salt and heavy metal stresses. Recombinant OsHSP24.1 protein was produced in E. coli cells for biochemical assay. The protein formed oligomeric complex when incubated with Sulfo-EGS (ethylene glycol bis (succinimidyl succinate)). Our results shows that Oshsp24.1 has an important role in abiotic stress response and have potential for developing stress-tolerant plants.

• 한국초지조사료학회지
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• 제42권3호
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• pp.208-214
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• 2022

Drought stress is a condition that occurs frequently in the field, it reduces of the agricultural yield of field crops. The aim of the study was to screen drought-adapted genotype of Italian rye grass. The experiments were conducted between the two Italian ryegrass (Lolium multiflorum L.) cultivars viz. Hwasan (H) and Kowinearly (KE). The plants were exposed to drought for 14 days. The results suggest that the morphological traits and biomass yield of KE significantly affected by drought stress-induced oxidative stress as the hydrogen peroxide (H₂O₂) level was induced, while these parameters were unchanged or less affected in H. Furthermore, the cultivar H showed better adaptation by maintaining several physiological parameter including photosystem-II (Fv/Fm), water use efficiency (WUE) and relative water content (RWC%) level in response to drought stress. These results indicate that the cultivar H shows improved drought tolerance by generic variation, improving photosynthetic efficiency and reducing oxidative stress damages under drought stress. These findings can be useful to the breeder and farmer for improving drought tolerance in Italian rye grass through breeding programs.

• 한국초지조사료학회지
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• 제9권1호
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• pp.56-61
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• 1989

本 연구는 濟州道內 放救用 野草地(마을共同收場)에서 野草類의 植生 및 收量性을 조사하여 草地改良 및 野草地의 利用度 增進을 위한 基鍵資料로 活用코자 放牧期間인 7 月부터 9 月까지 수행되었으며 quadrat method를 利用하여 조사한 結果는 다음과 같다. 1. 野草의 總草種은 64種으로 未本科 13草種, 두과 8草種, 엉거시科 12草種, 그리고 기타 31草 종으로 대부분 多年生 草類였다. 2.. 식생적性率은 띠 (Imperata cylindrica) 32.7%, 고사리 (Pteridium aquilinum) 18.9%, 억새 (Miscanthus sinensis) 8.4% 順이었고, 科別로는 未本科草種이 47.5%를 차지하였다. 3. 野草地의 l0a當 乾物收量은 7月이 160kg, 8月이 200-400kg, 9月 이 400-1,000kg으로 평균收量은 383 kg였으며, 生草收量은 평균 1,030kg으로 生育이 進行되면서 점차 증가하였다.

• 한국초지조사료학회지
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• 제17권3호
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• pp.293-304
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• 1997

A field experiment was conducted at the forage experimental plots, Seoul National University, Suweon h m 1995 to 1996 to determine the effect of legume residues as a N source and N fertilizer on corn (Zea mays L.) silage yield, N uptake, and availability of inorganic N in the soil. Corn was grown following (i) red clover (Trifolium pratense L.), (ii) crimson clover (Trifolium incarnatum L.), (iii) alfalfa (Medicago sativa L.) and (iv) winter fallow. The plots were split into two rates of fertilizer N (0 and 90kg Nlha) in a split-plot experimental plan. Compared with fallow treatment, legumes depleted soil water in the surface 15cm at corn planting by 17 to 26%. As a result, corn emergence was markedly delayed with legume residues by 8 to 11 days. Corn silage DM yield was significantly reduced in the presence of legume residues by 2.0 to 3.4 and 1.5 to 2.5 tonlha compared with winter fallow treatment at 0 and 90kg fertilizer Nlha, respectively, but no significant difference in the corn DM yield was found between legume residue treatments. There was an overall tendency for increased corn yields with 90kg fertilizer Nlha compared to Okg fertilizer Nlha, although not all yield increases were significantly greater. The corn yield response to applied N suggests that a source of N from legume residues was not sufficient for a succeeding corn crop. There was significantly more N (P