• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.419-432
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• 2010

This study was conducted to determine effects of applying cattle slurry and mixed sowing with legumes such as hairy vetch or forage pea on productivity, feed values and organic stock carrying capacity of whole crop barley and rye as winter forage crops, and to obtain organic forages together with higher soil fertility. Experimental plots consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (containing phosphate and potassium: P+K), chemical fertilizer (containing nitrogen, phosphate and potassium: N+P+K), organic fertilizer, cattle slurry, cattle slurry application (mixture with hairy vetch), and cattle slurry application (mixture with forage pea) plots. Each treatment was triplicates, and seven treatments were allocated in a completely randomized block design. For whole crop barley or its mixture crops, annual dry matter (DM), crude protein (CP), and total digestible nutrients (TDN) yields of N+P+K plots were significantly (P<0.05) higher than other plots except for cattle slurry plots. The CP content of barley or its mixture crops was significantly higher tor N+P+K plot (9.8%) and mixture plots with legumes (8.6~9.7%) than those of other treatments. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley or 70% mixture crops with legumes, mixture plots are capable of raising average 1.7 to 1.8 heads/ha a year. For rye or its mixture crops, annual DM, CP, and TDN yields represented 6.9~7.1, 0.5~0.6 and 4.3~4.4 ton/ha, respectively. The N+P+K plot contained 10.8% CP, which was higher (P<0.05) than all other treatments. In case of 450 kg Hanwoo heifers fed diets included 70% rye or 70% mixture crops with legumes, mixture plots can rear average 1.9 heads/ha a year. When it was considered based on crop yields and organic stock carrying capacity, applying cattle slurry to whole crop barley or rye had the comparable yields and feed values to chemical fertilizer application. Moreover, whole crop barley and rye within cattle slurry plots had a greater combination with hairy vetch and forage pea, respectively, and their mixture crops with legumes had higher crude protein and TDN yields within cattle slurry plots. In conclusion, it would be expected that mixed sowing with legumes in the application of cattle slurry to grass crops could be substituted for imported organic grains as dietary protein sources in feeding organic livestock.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.3
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• pp.325-333
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• 1994

This study was undertaken at Gowa, South Sulawesi, Indonesia as part of a larger forage genetic resource evaluation project. The experimental program consisted of a field experiment where grasses and legume species were grown in monocultures and the yield, N content and rumen bag digestibility using goats were measured. The field experiment was conducted from December 1985 to October 1986. Eight species of legumes (Desmodium heterophyllum cv. John-stone, Desmodium triflorum from Gowa, South Sulawesi, Arachis sp. from Maiwa, South Sulawesi, Clitoria ternalea CPI 50973, Macroptilium atropurpureum ev. Siratro, Neonotonia wightii cv. Tinaroo, Centrosema pubescens CPI 58575, Centrosema plumeri CPI 58568) were grown as monocultures. After establishment all plants were cut to a uniform height of 5 cm, and subsequent cuts were made on regrowth after 14, 28, 42, and 56 days (cycle 1). Cycle 2 commenced towards the end of the wet season and continued for 157 days into the dry season. The highest yielding legumes were C. ternatea in the wet season and Arachis sp. in the dry season. The mean rumen bag dry matter digestibility (RBDMD) of legumes of 67.6% for leaf material (averaged over all cycles and ages) was 7.6% higher than for stem material. The RBDMD of Arachis was significantly higher than all other species. The RBDMD of all legumes declined with age. Calculation of yield of digestible DM (yield $\times$ RBDMD) showed that Arachis sp. was the best legume. The combination of plant "quality" with yield measures is a valuable adjunct to routine agronomic survey procedures in plant evaluation programs. Arachis sp. appears to offer considerable promise and should be more widely evaluated.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.264-270
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• 1994

This experiment was undertaken to determine the effect of pasture ages on the total production and seasonal yield trends, botanical composition and forage quality of pasture mixtures. This experiment was conducted at the Livestock Experiment Station, RDA, Suweon. The results obtained were as follows : 1. Total dry matterDM) yield of pasture mixtures was the highest in the first year, and thereafter no significant reduction in the 2nd through the 4th year, but decreased by 30% in the 5th year. 2. The 34.4% of total DM yield was produced at the first cut, and decreased from then on. Total DM yield of pasture mixtures varied by year and climatic facton. In 1992, Total DM and TDN yields of pasture mixtures were 13.4 and 8.5 IvlT/ha, respectively. 3. The content of crude protein(CP), in virro DM digestibility(IVDMD) and TDN of pasture mixtures were 12-14, 70-80, and 62-65%, respectively. The highest forage quality was obtained at the 4th cutting. 4. The botanical compositions of legumes and weeds were increased as the year of establishment advanced, and highly increased at the 3rd cut after summer growth. 5. Based on the results of this study it appears that economic forage production could be expended to the 4th year with proper management, and the special renovation techniques may be needed for maintenance of pasture yield from the 5th year.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.41-47
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• 1992

Cheju Island has an optimal environment for herviborous animal production. However, the infrastructure has been degraded under the pressure from the development of tourism and thus the technological development for livestock production has not been great. This is the time to re-evaluate and re-direct the system of livestock production on the island. My suggestions on the development of grassland agriculture on the island are as follows : 1. Grassland area of Cheju, 65,695 ha should be maintained and the local government should try to improve more grasslands which can hold 3 or more cattle per ha on pasture. including enterpriseowned lands. 2. For better management of co-operatives village. the local government should evaluate the conditions of the grasslands yearly after being grazed and suggest a new management method accordingly. 3. In order to strengthen grassland research. it is recommended that Cheju Experiment Station be reorganized as a Grassland Research Institute or Department of Grassland Science be added. 4. [mport of good forage varieties. especially legumes, from foreign countries and distribution to farmers after adaption studies, are required to improve nutritive values of forage produced. 5. Methods for production of good quality forage seeds at rcasonablc prices should be established.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.127-132
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• 1999

No comprehensive study of yield and quality losses of forage legumes harvested and cured in spring has been conducted in Korea, therefore, this experiment was carried out to gain information on yield and quality, losses of alfalfa(Medicgo sativa L.), crimson clover (Trifolium incarnatum L.), and red clover(Trifolium pratense L.) during field curing in spring(mid-May). Alfalfa was highest in dry matter content of 15% at harvest, while crimson clover and red clover had 12.4 and 11.5%, respectively. Species differences for the dry matter content were maintained and consistent trends were observed during the field curing. Alfalfa took seven days to reach dry matter content over 80%, while both crimson clover and red clover took eight days. Crimson clover and alfalfa tended to show a higher leaf-stem ratio than red clover based on dry matter, but red clover showed a higher leaf-stem ratio than alfalfa and crimson clover based on crude protein content. Losses in dry matter of alfalfa from leaf shattering were higher than crimson clover and red clover, and similar trends were detected losses in crude protein for three different legumes. Fresh legume forages averaged higher in clude protein(CP), total digestible nutrients(TDN), and relative feed value(RFV), while dry legume forages averaged lower in acid detergent fiber(ADF) and neutral detergent fiber(NDF). Results of the experiment indicate that hay curing in spring affects crude protein concentration more than ADF and NDF, and this is due, in part, to leaf shattering caused by field operations.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.28-34
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• 1997

This study was conducted to determine the protein content, cell wall fractions, and mineral concentrations of some minor plant species collected for one year in pastures grazed by goats in the Philippines. An assessment of nutrient variability and a comparison of forage protein and mineral concentrations to the critical value of protein and minerals based on animal needs were also studied. The plant species were the following: grasses(Axonopus compressus, Eleusine indica, Rottboellia exaltata); legumes (Aeschynomene indica, Calopogonium muconoides, Desmodium tortousum); and herbs (Corchorus olitorius, Ipomea aquatica, Sida acuta, Synedrella nodiflora). The two seasons (dry and wet) were subdivided into Dry-1 (December to February, 132 mm total rainfall), Dry-2 (March to May, 25 mm total rainfall), Wet-1 (June to August, 1,138 mm total rainfall), and Wet-2 (September to November, 1,118 mm total rainfall). Results showed that significant differences were obtained on various nutrient fractions including those mineral concentrations across species. Across season, acid detergent lignin (ADL) had higher (p < 0.05) value at Dry-1. Legumes and herbs were higher in crude protein (CP) especially Sida acuta. Grasses showed the highest neutral detergent fiber (NDF) and acid detergent fiber (ADF) with the addition of Sida nodiflora (herb) for it contained high NDF. Aeschynomene indica contained the highest amount of ADL and the herbs (Ipomea aquatica and Sida acuta) had exceptionally high concentration of minerals. Coefficient variation of the various nutrient values ranged from 27.3 to 136.7%. Some forage minerals appeared to be deficient (sodium, phosphorus and copper) or excess (molybdenum) for the whole or part of the year. This study shows that some minor plant species could extend the range of concentration of some nutrients (i.e., CP and minerals) beyond that normally found in conventional pasture species.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.257-259
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• 1992

Three tropical legumes, very different in growth form, but believed to be of potential value for animal production were evaluated by substituting the leaf meal made from each for lucerne at the level used in a standard diet for growing rabbits (50%). Each leaf meal had a nitrogen content of close to 3.5% and contributed about 60% of the crude protein in the diet. Albizia lebbeck and Clitoria ternatea showed no evidence of toxic or antinutrient effects. The protein digestibility of the complete diets were 66 and 61% respectively, implying a protein digestibility of the leaf of at least 50%. Both species would be suitable for practical production diets for rabbits and should be excellent for ruminants. In contrast, the diet containing Desmanthus virgatus had a protein digestibility of only 40%, implying that only about 15% of the leaf protein was available. The leaves showed marked non-enzymic browning on drying. When dried this species is clearly unsuitable as a feed for rabbits and possibly also for ruminants. However, it may well be valuable as fresh forage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.2
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• pp.96-102
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• 1989

This field experiment was conducted to determine the effect of grazing and cutting management on the forage production and botanical composition at different legumes sown swards of the experimental field of Livestock Experiment Station. in Suweon, from Aug. 1985 to Oct. 1988. The results obtained are as follows: 1. Dry matter yield of cutting management (1213.0 kg/lOa) was higher by 19% than that of grazing management (1016.8 kg/lOa). Yield of pasture mixtures including alfalfa both cutting and grazing were higher than those including ladino clover and red clover. Yield under grazing was inferior to cutting in the first year but superior in the second year. 2. Seasonal dry matter production under grazing showed 5 1% in spring, 33% in summer and 16% in autumn, but under cutting showed 46% in spring, 42% in summer and 12% in autumn. 3. Daily dry matter production per 10a under grazing is decreased 5.9 kg in spring, 5.5 kg in summer and 3.7 kg in autumn. But under cutting is decreased 8.7 kg in summer, 6.6 kg in spring and 3.4 kg in autumn. 4. Botanical compositions of mixtures swards involving ladino clover and red clover were not influenced by method of defoliation. But mixtures swards including alfalfa were highly influenced by method of defoliation. Alfalfa ratio at the time of final utilization under grazing was about 30%, but under cutting was about 70-80%.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.1
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• pp.18-22
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• 1983

This experiment was performed in order to investigate the effect of the early grazing management on the forage yield and sward composition of oversown pasture. Two levels of the grazing intensity were treated when the grasses were 15cm, 25cm, and 35cm high. It was observed that the treatment C (light grazing intensity at the 25cm grass height) increased the dry matter yield and the botanical composition of the introduced grasses and legumes. From these facts it is suggested that the early grazing management contribute to the high forage yield and good sward composition of the oversown mountainous pasture and can take the place of cutting or other methods economically.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.187-192
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• 2014

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.