• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.192-197
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• 1995

This experiment was conducted to evaluate the grazing performance of dairy cattle for winter rye, sorghum-sudangrass hybrid and oat including of rape in Hwa seong-gun, Kyeonggido, from 1993 to 1994. Each crops were utilized from the stage of 8-leaves to late blooming for sorghum-sudangrass, and from final leaves to soft dough for winter rye and oat plant. Under three different cropping system of winter rye-sorghum x sudangrass, spring oat-sorghum X sudangrass-autumn oat, and silage corn-autumn oat, a succeshl hehage production was available for grazing performance from April 26 to December 15, continuously. Annual dry matter yields for grazing allowance were 26.1 tontha in average of all cropping system. Autumn oat rnixtured with rape showed the highest herbage utilization with 91.6% grazing intensity and the lowest in sorghum-sudangrass with a value of 60.6- 69.1 %. Labor investment for herbage production were 805 hour in the cutting management and 339 hour per hectar in the grazing utilization.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.2
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• pp.135-140
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• 1997

This study was conducted to investigate the effect of levels of nitrogen fertilizer on the nitrate accumulation in Italian ryegrass. Italian ryegrass was grown at the experimental field, College of Agriculture, Chonnam National University, Kwangju from September, 29, 1987 to June, 24, 1988. The experiment was arranged as a randomized complete block design with three treatment(2.5, 7.5 and 15kg-NIlOa). The results obtained are summarized as follow; Nitrate nitrogen content of the stem, leaves and whole was increased as increasing nitrogen fertilizer(P< 0.05). Total nitrogen content in the leaves was much higher than that of stem, and not significance among the levels of nitrogen fertilizer. Total water soluble carbohydrate content in stem was higher than that in the leaves and decreased after cutting as increasing nitrogen fertilizer(P < 0.05)

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.3
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• pp.170-174
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• 2018

Environmental stresses caused by climate change, such as high temperature, drought and salinity severely impact plant growth and productivity. Among these factors, high temperature stress will become more severe during summer. In this study, we examined physiological and molecular responses of maize plants to high temperature stress during summer. Highest level of $H_2O_2$ was observed in maize leaves collected July 26 compared with June 25 and July 12. Results indicated that high temperature stress triggers production of reactive oxygen species (ROS) in maize leaves. In addition, photosynthetic efficiency (Fv/Fm) sharply decreased in leaves with increasing air temperatures during the day in the field. RT-PCR analysis of maize plants exposed to high temperatures of during the day in field revealed increased accumulation of mitochondrial and chloroplastic small heat shock protein (HSP) transcripts. Results demonstrate that Fv/Fm values and organelle-localized small HSP gene could be used as physiological and molecular indicators of plants impacted by environmental stresses.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.3
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• pp.237-241
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• 2017

Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.3
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• pp.190-195
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• 2018

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.223-230
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• 1997

The experiments were undertaken to investigate the dynamics of $NO_3\;^-$ uptake at low temperature with forage rape(Bmssica napus), barle(Hordeum vulgare) and winter rye (Scale cereale). A net $NO_3\;^-$uptake and the contents of nitrogenous compound in tissues at low temperature ($5^{\circ}C$) were compared with normal temperature ($25^{\circ}C$).The net uptake of $NO_3\;^-$ by Brass ica nqus was the highest among three species at both temperature applied. During 9 hours after temperature treatment, $NO_3\;^-$ uptake of Brassica napus, Hordeum vulgm and Scale c e d e grown at $5^{\circ}C$ decreased to about 31, 50 and 27% compared with those grown at $25^{\circ}C$. $NO_3\;^-$ contents in roots were much sensitively affected by low temperature than those of leaves. Comparing with the plants grown at $25^{\circ}C$, $NO_3\;^-$ contents in roots of increased to 48.3, 38.7 and 53.5% repectively, for Brassica napus, Hordeum vulgare and Scale cereale during 9 days. A relatively less changes in the content of $NH_4\;^+$-N were observed according to the temperature treatment. The ontents of amino acids-N in leaves at $5^{\circ}C$ slightly decreased while that of roods remarkably increased compared with $25^{\circ}C$. Protein-N was the largest pool of nitrogen and its contents in leaves and roots showed a similar response on the temperature treatment. These results suggested that $NO_3\;^-$uptake was inhibited at low temperature. The dynamics of $NO_3\;^-$ uptake could be closely related to the increased concentration of $NO_3\;^-$ and amino acids in roots.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.183-187
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• 2022

Alfalfa is one of the most useful forage crops worldwide, containing a high level of amino acids that are essential to both human and animal health. However, amino acids and their concentrations may differ between plant parts. Hence, detecting amino acids in different plant parts would be useful in the development of diet supplements. The purpose of this study was to determine the amino acid content in alfalfa leaves, stems, and inflorescences using an amino acid analyzer. Asparagine and glutamic acid were the most abundant amino acids found in stems, leaves, and inflorescences than other amino acids. All parts of alfalfa had low concentrations of cysteine and methionine. All amino acids except asparagine were present in the highest concentration in leaves followed by inflorescences. Leaf had a rich amino acid content, namely asparagine, glutamic acid, leucine, proline, and lysine. However, the stem had a lower amino acid composition than the leaf or inflorescence. Overall, the data showed determining the amino acid content of forages provides a good approach to making animal feed with essential and specific amino acids and preventing excessive inclusion of amino acids.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.169-178
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• 2014

Samples of Clitoria ternatea L. (Cunh$\tilde{a}$) were harvested at 35, 50, 70, and 90 d after a uniformity harvest in a field study designed as a completely randomized design with a total of 18 experimental plots. The dry matter yield of the whole plant was separated quantitatively into leaves, stems, and pods at each harvesting age. Chemical analyses and in vitro gas production kinetics were performed to assess the quality of the plant parts. Yields, chemical composition, and estimates of gas production parameters were analyzed by fitting a mixed statistical model with two types of covariance structures as follows: variance components and an unrestricted structure with heterogeneous variances. Fast and slow gas yielding pools were detected for both leaves and stems, but only a single pool was detected for pods. The homoscedasticity assumption was more likely for all variables, except for some parameters of the gas production kinetics of leaves and stems. There was no presence of typical pods at 35 and 50 d. In the leaves, the fibrous fractions were affected, whereas the non-fibrous fractions were unaffected by the harvesting age. The harvesting age affected the majority of the chemical constituents and gas kinetic parameters related to the stems. The leaves of this legume were the least affected part by the aging process.

• Korean Journal of Organic Agriculture
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• v.28 no.4
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• pp.615-626
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• 2020

The objective of present experiment was to examine the impact of different organic nitrogen (N) fertilizer application rates and different harvesting periods on the forage yield and feed value of kenaf (Hibiscus cannabinus L.). This study was carried out from May to September 2019, the amount of 80 kg of kenaf seed/ha were applied with different rates of nitrogen fertilizer. The plants were sampled at 10 days intervals (100, 110, 120, and 130 days after seeding) from different harvesting dates. In the organic fertilizer treatments, the highest dry matter (DM) yield was observed in the application rate at 250 kg of N/ha. Crude protein (CP) content in leaves was similar between the organic fertilizer rates at 200 and 250 kg of N/ha and were higher compared with other fertilizer treatments. The highest CP content in the stem was 4.3% in the organic fertilizer application rates st 250 kg of N/ha. Neutral detergent fiber (NDF) in leaves showed no significant difference between the chemical fertilizer rates at 200 and 250 kg of N/ha, and the organic fertilizer rates at 150 and 250 kg of N/ha. In addition, DM yield of kenaf was highest in the harvest of 100 days after seeding, and tended to decreased significantly with increase of harvesting periods (p<0.05). As the growth progressed, the plant height and stem ratio increased but the leaves ratio decreased significantly (p<0.05) and the highest was found at 110 days after seeding. The highest CP, Acid detergent fiber (ADF), NDF and total digestible nutrient (TDN) contents in leaves were 13.9, 25.4, 40.5 and 71.1%, respectively that were affected by different harvesting periods. Also, the ADF and NDF in stem increased significantly with increase of kenaf maturity (p<0.05). In conclusion, the optimal organic fertilizer application rates and the proper harvesting periods for the forage yield and quality of kenaf were at 200 to 250 kg of N/ha, and 100 to 110 days after seeding, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.162-167
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• 2023

The experiment aim to breed an early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.). It was conducted at the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea, from 2022 to 2023. The new variety named "Oasis" is a diploid with green leaves and has a semi-erect growth habit in late fall, and an erect growth habit in early spring. With a heading date of May 10, Oasis is categorized as an early-maturing variety. Compared with the "Florida80" as a control variety, Oasis's flag leaf was 1 mm wider and 2 cm shorter, while the plant length was 7.8 cm longer. Additionally, the ear of Oasis was 1.2 cm longer than that of Florida80, and it showed lodging resistance. The dry matter yield of Oasis (8,805 kg/ha) was higher than that of Florida80. The total digestible nutrient and crude protein contents of Oasis were 64.5%, and 9.3%, respectively, which were 1.1% and 0.6% higher than those of Florida80, respectively. The neutral and acid detergent fiber contents in Oasis were 54.2% and 30.8%, respectively, which was 1.9% and 1.4% lower than those of Florida80, respectively. These results indicate that Oasis has good dry matter yield in the most of region and especially, middle and northern region of South Korea.