• 한국동물위생학회지
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• 제41권1호
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• pp.29-39
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• 2018

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

• 한국산학기술학회논문지
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• 제12권11호
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• pp.5063-5070
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• 2011

구제역은 소 돼지, 양, 염소 및 사슴 등의 발굽이 두 개인 가축 또는 야생 동물에서 발생하는 질병이다. 본 연구는 구제역에 대한 지식, 인식과 생활 습관을 경기도 총 5개 지역 초등학생 398명을 대상으로 조사하였다. 구제역이 무엇인지 들어보았는지 조사한 결과 구제역 교육을 받은 그룹은 49명 92.5%가 '있다'고 하였으며, 구제역 교육 받지 않은 그룹은 139명 68.5%가 '있다'고 하였다. '구제역 바이러스는 섭씨 $50^{\circ}C$ 이상에서 파괴되기 때문에 조리시 모두 바이러스 균이 모두 죽는다'고 지식을 조사한 결과 구제역 교육 받은 그룹은 26명 49.1%, 구제역 교육을 받지 않은 그룹은 82명 40.4%만 올바르게 알고 있는 것으로 나타났다. 구제역과 같은 질병에 대한 교육은 언제하는 것이 좋다고 생각하는지 조사한 결과 두 그룹 모두 초등학교 시기라고 응답하였다. 구제역은 다국가간에 수차례 감염 발생이 일어나고 있는 질병으로 우리나라도 안전하지 못한 상재지역이다. 이에 국민들이 올바른 인식과 지식으로 대비해야 할 것이다.

• 한국산학기술학회논문지
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• 제17권9호
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• pp.292-301
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• 2016

구제역 발생에 따른 피해는 매우 크기 때문에 구제역의 피해를 최소화하기 위해서는 선제적인 구제역 진단 및 대응이 필수적이다. 주요 구제역 증상은 소의 체온 상승, 식욕 부진, 유량 감소, 입 발굽 유방에 물집 형성 등이며, 이중 확인하기 가장 쉽고 빠른 방법은 체온을 검사하는 방법이다. 본 논문에서는 선제적 구제역 대응을 위해 소 발굽 검출 알고리즘을 개발 구현하고, 축사에 고해상도 카메라 모듈과 열화상 카메라, 온습도 모듈 설치하여 발굽 검색 테스트를 시행하였다. 본 연구에서 개발한 알고리즘과 시스템을 통해 구제역 의심 가축의 조기 상황 대처를 할 수 있으며, 가축의 최적 성정 환경을 조성할 수 있다. 특히, 본 연구에서는 기존의 휴대용이 아닌 열화상 카메라를 활용한 구제역 대응 시스템은 축사에 고정으로 부착하여 별도 인력을 필요로 하지 않고 이미지 알고리즘을 통하여 가축의 발굽 온도를 자동 측정하는 기능과 스마트 폰을 활용한 자동 경고 기능을 가지고 있다. 이러한 시스템은 실시간을 구제역 가능성 예측을 가능케 하며, 별도의 인력이 없이 가축 질병에 대한 초동 방역 대응을 할 수 있다.

• 한국멀티미디어학회논문지
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• 제20권3호
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• pp.474-481
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• 2017

In Korea, foot-and-mouth disease has not been reported for several decades, but it began to develop again in 2000. For 2010~2011, when the worst occurred, 3.5 million animals were disposed of resulting in a loss of 2.8 trillion won. In order to prevent the harmful effects of foot-and-mouth disease, vaccination and housing management are being implemented. Despite these measures, foot-and-mouth disease is infected with air through the respiratory tract and accompanies fever after latency. Therefore, it is recognized that measuring and managing the body temperature of livestock at the early stage is the first step of managing this disease. In this paper, we propose a temperature monitoring system that can measure the body temperature by incorporating temperature sensor mounted in ear-tag of cattle and collect body temperature data of each individual cattle through BLE into the control server. The proposed body temperature monitoring system has various advantages such as easy installation without the help of livestock specialists and not damaging the organs of the livestock. So, it is possible to manage the abnormal symptom of cattle in real time and it is believed that the proposed monitoring system will revolutionize the prevention of foot-and-mouth disease.

• 한국동물위생학회지
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• 제34권2호
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• pp.95-102
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• 2011

Foot-and-mouth disease (FMD) is one of the most infectious diseases affecting cloven-hoofed animals including cattle, sheep, goats, and pigs. Seven serotypes of foot-and-mouth disease virus with multiple subtypes within each serotype have been identified until now. In particular, it has been demonstrated that the outbreak of the serotype Asia1 reported from China, Mongolia and North Korea since 2005 is mostly classified into genetic group V. Though it has been recommended that Asia1 Shamir strain can be used as a high priority vaccine by World References Laboratory for FMD, the complete nucleotide sequences of the strain has not yet been determined. In this study, to be prepared for Asia1 type viruses that may be brought into Korea, the complete genome sequence of this vaccine strain Asia1 Shamir including its 5' and 3' non-coding region was identified.

• 한국시스템다이내믹스연구
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• 제14권4호
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• pp.37-62
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• 2013

The purpose of this paper is to find out some clues about effectiveness and implementing timing of the control measures against Foot-and-Mouth Disease (FMD) in Korea. To do that, the case of FMD, which broke out during November 2010 in Korea, is examined and constructed as a system dynamics simulation model. The implications of simulation result are as follows. First of all, it is most effective measure to prevent the movement of vehicle from infected farmhouse to other noninfected farmhouses, which are located far away, in the early stage of FMD diffusion. Secondly, earlier vaccination can be adopted as a reliable means to control the epidemic when FMD spreads widely. Finally, reducing the time to vaccinate the whole noninfected livestock is a little help to restrain the additional infections of FMD.

• 한국임상수의학회지
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• 제21권2호
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• pp.172-176
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• 2004

We described the clinical signs, gross lesions, histopathological lesions, and electronmicroscopy of pig Foot-and-Mouth Disease cases which had occurred in 2002, Korea. Grossly affected pigs showed vesicles on snout, tongue, coronary band, and udder. Histoapthologically, severe intercellular edema and necrosis of prickle cells, and separation between epidermis and dermis were found on the mucosa of tongue and coronary band. And myocardial necrosis and mononuclear cells infiltration in myocardium were found. Electronmicroscopically numerous picornavirus particles(18∼22 nm in diameter) were found in the cytoplasm of prickle cells. By those results, we confirm that those cases were typical FMD cases.

• 한국자기공명학회논문지
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• 제7권2호
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• pp.89-97
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• 2003

Foot-and-mouth disease virus (FMDV) belongs to the aphthovirus genus within the picornavirus which has a single copy of a positive sense RNA. The translation initiation process of FMDV occurs by a cap-independent mechanism directed by a highly structured element (∼435 nt) termed an internal ribosome entry site (IRES). We have designed and prepared FMDV 4-2 RNA (28nt) by in vitro transcription. The 2D NMR data revealed that FMDV 4-2 IRES domain RNA has a flexible loop and bulge conformation. In further study, we need to make an isotope labeled RNA sample and conduct 3D NMR experiments to completely determine the 3D structure. This study may establish a new drug design strategy to treat foot-and mouth disease.

• 미생물학회지
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• 제53권4호
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• pp.304-308
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• 2017

구제역 바이러스 연구가 BSL-3 시설에 제한되기 때문에 여러 가지 소독제나 항바이러스 제제에 대한 효력 및 효능 평가가 쉽게 이루어질 수 없다. 따라서 구제역 바이러스와 계통학적으로 유사한 bovine rhinovirus (BRV)와 human rhinovirus (HRV)의 특성을 열, pH 그리고 여러가지 소독제를 이용하여 평가하였다. 그 결과 구제역 바이러스의 성상과 매우 흡사한 것을 확인할 수 있었다. 이러한 결과로 BRV와 HRV는 구제역 바이러스를 대체할 수 있는 모델 바이러스로 이용이 가능하다.

• 한국동물위생학회지
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• 제41권1호
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• pp.9-13
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• 2018

The best way to prevent foot-and-mouth disease (FMD) constantly occurring in Korea will be vaccination. In this study, FMD vaccines were given to Korean native cattle (Hanwoo), dairy cattle, and pigs to investigate the antibody positive rate of FMD vaccine by age in year and month. Hanwoo, dairy cattle, and pigs showed antibody positive rates of 99.5%, 97.7%, and 95.9%, respectively. High antibody positive rates more than 95% were found in Hanwoo and dairy cattle. In particular, high antibody positive rates were found in Hanwoo and dairy cattle regardless of age. Pigs showed a relatively low antibody positive rate of 57.6% at 3 months of age and then constantly maintained a high antibody positive rate of above 95.0% after 4 months of age. As a result of this study, high antibody positive rates were found when regular FMD vaccination was given to newborn calves and piglets after FMD vaccination twice to them. Therefore, it is considered the most important to receive vaccination thoroughly according to vaccination plan in order to prevent FMD.