• 한국식품영양학회지
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• 제30권2호
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• pp.356-362
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• 2017

The aims of this study was to investigate the satisfactions with school food service and its influencing factors for elementary and middle school parents. We surveyed parents regarding their concerns about food ingredients and dietary life, perceptions of free school food service, the necessity of food supply systems such as school food service centers, and the satisfaction about the quality and management of school food services. Compared to the last year, concerns about food ingredients and dietary life as well as perceptions about free school food services decreased. The reported necessity of supply system such as school food service centers and co-purchasing was lower than in 2015. The satisfaction about the school food service decreased, compared to 2015. More individuals had higher concerns about local food and stated the necessity for school food supply systems, as well as a higher satisfaction about the quality and management of school food services. In conclusion, it is important to emphasize characteristics of school food services such as co-purchasing local foods.

• 한국식품영양학회지
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• 제30권6호
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• pp.1245-1251
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• 2017

The objective of this study is to investigate the satisfaction of elementary, middle and high school nutrition teachers in the Chungnam province concerning the free school food service. We surveyed satisfaction related to policies on school food service and the school food service center. Satisfaction on the school food service center was separated according to four dimensions; perception, food materials, operational system and service. Furthermore, we analyzed factors that affect satisfaction with the school food service center with a multiple regression model. First, satisfaction about the free school food service and dietary life education are higher than the total average of satisfaction with the policy about the school food service. Second, satisfaction about a variety of food materials and reasonable prices are lower than total average of satisfaction with the school food service center. Third, when more teachers have a high level of a positive perception and have higher satisfaction with the operational system of the school food service center, then they also subsequently display higher satisfaction with the school food service center. Therefore it is necessary to improve or make up for pricing and diversity on food materials by the school food service center.

• 한국수산과학회지
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• 제50권5호
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• pp.473-480
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• 2017

We investigated the nutritional characteristics of the uncooked Skipjack tuna Katsuwonus pelamis frame (U-STF) as a basic resource for preparing flavor-enriching concentrates. The bone rate based on the fish frame (FF) was 53.2% in U-STF, thus lower than those of cooked skipjack tuna frame (C-STF), the uncooked salmon frame (U-SF), and beef leg bone (BLB). The composition of FF had 48.6-58.0% moisture, 18.4-21.3% crude protein, 7.0-15.0% crude lipid, and 12.8-22.1% ash. Compared to the proximate composition of BLB, that of FF was higher moisture and crude protein but lower solids, crude lipids, and ash. The total amino acid contents of FF was 17.6-20.8 g/100 g, which was higher than that of BLB. The levels of calcium and phosphorus of FF were 4.7-8.5 g/100 g and 2.4-4.2 g/100 g, respectively, thus lower than those of BLB. The brix concentration of FF extract was $5.0-8.2^{\circ}$, being highest in U-STF extracts, followed by extracts of U-SF and then extracts of C-STF. Our results indicate that U-STF is an optimal resource for preparing flavor-enriching concentrates.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.71-71
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• 2018

본 연구는 각시톱시네고사리(Dryopteris hangchowensis Ching)의 전엽체 증식 및 포자체 형성을 위한 번식방법을 구명하고자 수행되었다. 각시톱지네고사리의 식물체를 무가온 온실에 재배하면서 포자를 채취하였으며, 수집된 포자는 기내에서 발아시켜 전엽체를 획득한 다음 계대배양하면서 실험의 재료로 사용하였다. 전엽체 대량증식을 위하여 1/4, 1/2, 1배로 조절한 MS배지와 Knop배지를 조성하였다. 이후 전엽체 300mg을 균일하게 다져 조성된 배지에 8주간 배양하였다. 연구의 결과, 전엽체의 생체중은 1/2MS와 1/4MS에서 3.4g으로 가장 많이 증가하였다. 현미경 관찰의 결과, 두 배지 모두 정상적인 전엽체의 형태로 발달하였으며, 생육도 양호하였다. 포자체 형성을 위한 적정 배양토의 혼합조건을 확인하고자 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하였다. 준비된 배양토를 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 후 기내 배양한 전엽체 1g과 증류수를 함께 10초간 분쇄하여 토양표면에 균일하게 분주한 다음 12주간 재배하였다. 연구의 결과, 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v) 비율로 혼합한 토양에서 포트 당 223.7개로 가장 많은 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과, 상기 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 각시톱시네고사리의 전엽체 증식에 적합한 배지는 경제성과 생육수준을 고려하여 1/4MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v)로 혼합한 토양이 적합하다고 판단되었다.

• 한국축산식품학회지
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• 제37권3호
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• pp.402-409
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• 2017

A novel peptide having free radical scavenging activity was separated, using an on-line high-performance liquid chromatography (HPLC) - ABTS screening method, from bovine skim milk fermented by Lactococcus lactis SL6 (KCTC 11865BP). It was further purified using reverse phase-HPLC (RP-HPLC) and sequenced by RP-HPLC-tandem mass spectrometry. The amino acid sequence of the identified peptide was determined to be Phe-Ser-Asp-Ile-Pro-Asn-Pro-Ile-Gly-Ser-Glu-Asn-Ser-Glu-Lys-Thr-Thr-Met-Pro-Leu-Trp (2,362 Da), which is corresponding to the C-terminal fragment of bovine ${\alpha}_{s1}$-casein (f179-199). The hydroxyl radicals scavenging activity ($IC_{50}$ $28.25{\pm}0.96{\mu}M$) of the peptide chemically synthesized based on the MS/MS data showed a slightly lower than that of the natural antioxidant Trolox ($IC_{50}$ $15.37{\pm}0.52{\mu}M$). Furthermore, derivatives of the antioxidant peptide were synthesized. The antioxidative activity of the derivatives whose all three proline residues replaced by alanine significantly decreased, whereas replacement of two proline residues in N-terminal region did not affect its antioxidative activity, indicating that $3^{rd}$ proline in C-terminal region is critical for the antioxidative activity of the peptide identified in this study. In addition, N-terminal region of the antioxidant peptide did not show its activity, whereas C-terminal region maintained antioxidative activity, suggesting that C-terminal region of the peptide is important for antioxidative activity.

• 한국축산식품학회지
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• 제35권5호
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• pp.653-659
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• 2015

High-protein fermented whey beverage (FWB) was manufactured using whey protein concentrate (WPC) and Lactobacillus plantarum DK211 isolated from kimchi. This study was designed to evaluate the anti-obesity activity of FWB in male rats fed a high-fat diet. Male Sprague-Dawley rats were randomly assigned to three groups (n=8 per group). The three groups differed in their diet; one group received a normal diet (ND), another, a high-fat diet (HD), and the third, a HD plus fermented whey beverage (HDFWB), for 4 wk. Supplementation with FWB (the HDFWB group) prevented weight gain and body fat accumulation. The food intake in the HDWFB group was significantly lower (p<0.05) than that of the HD group. The HDWFB group also showed a significant decrease in organ weights (p<0.05), except for the weight of the testis. There was a significant decrease in total cholesterol, LDL-cholesterol, and triglycerides in the HDFWB group compared with the HD group (p<0.05), but there was no significant difference in serum HDL-cholesterol levels among the experimental groups. Rats ingesting FWB (the HDFWB group) also showed a significant decrease in blood glucose levels, and plasma levels of insulin, leptin, and ghrelin compared to HD group (p<0.05). These results indicate that FWB has beneficial effects on dietary control, weight control, and reduction in fat composition and serum lipid level; consequently, it may provide antiobesity and hypolipidemic activity against high fat diet-induced obesity in rats.

• 한국축산시설환경학회지
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• 제11권1호
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• pp.61-70
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• 2005

본 실험은 남은 음식물에 유기성 부산물인 육계분, 제과부산물, 밀기울, 맥강 등을 주요 수분흡수제 또는 영양 보충제로 혼합한 후 rotary drum 방식을 이용하여 양돈 사료화 하는데 있어서 이상적인 배합비 도출을 위한 공정단계별 사료의 영양적 flow를 4차 시험에 걸쳐서 추적하였다. Rotary dm 방식을 이용한 살균 및 숙성 과정을 거치면서 영양소의 변화 폭은 매우 낮았으며, 살균 및 숙성 처리 후의 건물 손실율은 $8{\~}15\%$ 수준이었다. 시험별로 영양소 변화는 혼합되는 사료 원료의 종류에 따라 다르게 나타났다. 남은 음식물, 퇴적발효육계분, 제과부산물, 맥강의 최적 혼합 비율은 45: 10: 22.5:22.5 이었고, 이때 사료간의 뭉쳐지는 현상을 극복할 수 있었다. 결과적으로 rotary durm 방식으로 살균하고, 숙성한 남은 음식물 혼합물은 양돈 사료로서의 충분한 사료 영양적 가치가 있었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.44-44
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• 2019

This study aimed to develop a suitable method for inducing the proliferation of prothallus and producing sporophytes of rock polypody (Polypodium vulgare L.). The prothalli used in all experiments were obtained from spore germination and sub-cultured for 8-week intervals. The most appropriate media for prothallus propagation were investigated by culturing 300 mg of prothallus in MS ($1/4{\times}$, $1/2{\times}$, $1{\times}$, and $2{\times}$ strength) medium and in Knop medium for 8 weeks. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\pm}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark). Fresh weight of prothalli was 4.8 g on $1{\times}$ MS, 4.5 g on $1/2{\times}$ MS and 4.3 g on 1/4 MS medium. To select a suitable soil combination for sporophyte formation, 1.0 g of prothallus was ground with distilled water, spread in five combinations onto different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and then cultivated for 13 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). The results showed that a mixture containing a 2:1 (v:v) ratio of horticultural substrate and perlite, increased sporophyte formation to 462.5 sporophytes per pot (7.5 cm2). The other soil substrates produced from 314.5 to 405.3 sporophytes per pot. Therefore, our results will provide conditions suitable for mass production of Polypodium vulgare L.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.57-57
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• 2019

실내 수준에서 조사되는 광량조건에 따른 자생 상록활엽 2종의 생육과 광화학반응을 조사하였다. 식물재료는 3년생 사철나무(Euonymus japonicus Thunb.) 및 2년생 돈나무[Pittosporum tobira (Thunb.) W. T. Aiton] 실생묘로 유리온실에서 재배하면서 실험에 사용하였다. 실내 광량은 10, 50, 100 및 200 PPFD(${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 달리하여 8주간 재배하였으며, 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$), 습도($55{\pm}3%$) 및 관수(1회/3일)조건은 고정되었다. 생육특성 및 광화학반응의 요인들이 측정되었으며, 동일기간 동안 유리온실에서 재배된 식물을 대조구로 사용하였다. 실험의 결과, 사철나무는 100, 200 PPFD의 광량에서 대조구에 비해 초장, 줄기직경, 엽수 및 엽장의 생육반응이 우수하였으며, 엽록소함량은 100 PPFD 이상의 광량에서 감소하였다. 또한 100 PPFD 이상의 광량에서 스트레스지수(Fm/Fo), 최대양자수율(Fv/Fm) 및 전자전달효율(ETo/RC)이 감소하였으며, 이는 대조구와도 유사한 경향이었다. 돈나무는 모든 광량조건에서 줄기직경과 엽폭을 제외하고 대조구와 관계없이 생육반응이 일정하였다. 그러나 스트레스지수와 최대양자수율은 10 PPFD 처리구에서 가장 낮게 조사되었고, 비광학적 에너지의 손실(DIo/RC)은 2.53으로 가장 높았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.58-58
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• 2019

본 연구에서는 자생 참나무속 2종의 몇 가지 저광량 조건하에서 생육 및 엽록소 형광 반응을 조사하였다. 연구의 재료는 참가시나무(Quercus salicina Blume)와 붉가시나무(Q. acuta Thunb.)의 2년생 실생묘를 사용하였다. 다양한 실내 광환경조건을 조사하여 광량을 10, 50, 100 및 200 PPFD (${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 설정하였으며, 실험기간 동안 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$) 및 습도($55{\pm}3%$)를 동일하게 설정하여 총 8주간 재배하였다. 대조구는 유리온실에서 동일기간 동안 재배된 식물을 사용하였다. 연구의 결과, 참가시나무는 50 PPFD 이하에서 전체 식물 중 50%가 잎이 마르며 떨어지는 경향을 보였으며, 100 PPFD에서도 25%의 잎마름이 관찰되었다. 200 PPFD는 줄기직경을 제외한 초장, 엽수, 엽장, 엽폭 및 엽록소 함량(SPAD)이 대조구와 유사하거나 높았으며, 엽록소 형광 반응에서도 모든 측정값이 대조구와 유의한 차이를 보이지 않았다. 붉가시나무는 50 PPFD 이상의 광량에서는 줄기직경, 엽수, 엽장 및 SPAD가 대조구와 유사하거나 높은 결과를 보였으나, 모든 광량조건에서 초장과 엽폭이 대조구에 비해 유의적으로 낮았다. 엽록소 형광 반응은 모든 광량에서 대조구와 비슷한 양상을 보였으나, 10 PPFD는 초기형광값(Fo)과 비광학적 에너지의 손실(DIo/RC)이 대조구에 비해 유의적으로 낮은 결과를 보였다.