• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2897-2901
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• 2012

Invasion is usually recognized as the main reason for the high recurrence and death rates of glioma and restricts the efficacy of surgery and other therapies. Therefore, we aimed to investigate the mechanism involved in promotion effects of mda-9/syntenin on human glioma cell migration. The wound healing method was used to test the migration ability of human glioma cells CHG-5 and CHG-hS, stably overexpressing mda-9/syntenin. Western blotting was performed to determine the expression and phosphorylation of focal adhesion kinase (FAK) and JNK in CHG-5 and CHG-hS cells. The migration ability of CHG-hS cells was significantly higher than that of CHG-5 cells in fibronectin (FN)-coated culture plates. Phosphorylation of FAK on tyrosine 397, 576, and 925 sites was increased with time elapsed in CHG-hS cells. However, phosphorylated FAK on the tyrosine 861 site was not changed. Phosphorylated Src, JNK and Akt levels in CHG-hS cells were also significantly upregulated. Phosphorylation of JNK and Akt were abolished by the specific inhibitors SP600125 and LY294002, respectively, and the migration ability of CHG-hS cells was decreased, indicating that the JNK and PI3K/Akt pathways play important roles in regulating mda-9/syntenin-induced human brain glioma migration. Our results indicate Mda-9/syntenin overexpression could activate FAK-JNK and FAK-Akt signaling and then enhance the migration capacity of human brain glioma cells.

• International Journal of Oral Biology
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• 제37권4호
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• pp.189-195
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• 2012

Resistance to the induction of apoptosis is a possible mechanism by which tumor cells can survive anti-neoplastic treatments. Melanoma is notoriously resistant to anti-neoplastic therapy. Previous studies have demonstrated focal adhesion kinase (FAK) overexpression in melanoma cell lines. Given its probable role in mediating resistance to apoptosis, many researchers have sought to determine whether the downregulation of FAK in melanoma cells would confer a greater sensitivity to anti-neoplastic agents. Genistein is a known inhibitor of protein-tyrosine kinase (PTK), which may attenuate the growth of cancer cells by inhibiting the PTK-mediated signaling pathway. This present study was undertaken to investigate the effect of genistein on the expression of FAK and cell cycle related proteins in the G361 melanoma cell line. Genistein was found to have a preferential cytotoxic effect on G361 melanoma cells over HaCaT normal keratinocytes. Genistein decreased the expression of 125 kDa phosphotyrosine kinase and the FAK protein in particular. Genistein treatment did not affect the expression of p53 in G361 cells in which p21 is upregulated. The expression of cyclin B and cdc2 was downregulated by genistein treatment. Taken together, our data indicate that genistein induces the decreased proliferation of G361 melanoma cells via the inhibition of FAK expression and regulation of cell cycle genes. This suggests that the use of genistein may be a viable approach to future melanoma treatments.

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.383-389
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• 2017

Dexmedetomidine is an ${\alpha}2$-adrenergic receptor agonist that exhibits a protective effect on ischemia-reperfusion injury of the heart, kidney, and other organs. In the present study, we examined the neuroprotective action and potential mechanisms of dexmedetomidine against ischemia-reperfusion induced cerebral injury. Transient focal cerebral ischemia-reperfusion injury was induced in Sprague-Dawley rats by middle cerebral artery occlusion. After the ischemic insult, animals then received intravenous dexmedetomidine of $1{\mu}g/kg$ load dose, followed by $0.05{\mu}g/kg/min$ infusion for 2 h. After 24 h of reperfusion, neurological function, brain edema, and the morphology of the hippocampal CA1 region were evaluated. The levels and mRNA expressions of interleukin-$1{\beta}$, interleukin-6 and tumor nevrosis factor-${\alpha}$ as well as the protein expression of inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-${\kappa}Bp65$, inhibitor of ${\kappa}B{\alpha}$ and phosphorylated of ${\kappa}B{\alpha}$ in hippocampus were assessed. We found that dexmedetomidine reduced focal cerebral ischemia-reperfusion injury in rats by inhibiting the expression and release of inflammatory cytokines and mediators. Inhibition of the nuclear factor-${\kappa}B$ pathway may be a mechanism underlying the neuroprotective action of dexmedetomidine against focal cerebral I/R injury.

• Reproductive and Developmental Biology
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• 제35권2호
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• pp.167-174
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• 2011

Acute ischemic stroke results from sudden decrease or loss of blood supply to an area of the brain, resulting in a coinciding loss of neurological function. The antioxidant action of melatonin is an important mechanism among its known effects to protective activity during ischemic/reperfusion injury. The focus of this research, therapeutic efficacy of melatonin on recovery of neurological function following long term treatment in ischemic brain injured rats. Male Sprague-Dawley rats (n=40; 8 weeks old) were divided into the control group, and MCAo groups (Vehicle, MT7 : MCAo+ melatonin injection at 7:00, MT19 : MCAo+melatonin injection at 19:00, and MT7,19 : MCAo+melatonin injection at 7:00 and 19:00). Rat body weight and neurological function were measured every week for 8 weeks. After 8 weeks, the rats were anesthetized with a mixture of zoletil (40 mg/kg) and xylazine (10 mg/kg) and sacrificed for further analysis. Tissues were then collected for RNA isolation from brain tissue. Also, brain tissues were analyzed by histological procedures. We elucidated that melatonin was not toxic in vital organs. MT7,19 was the most rapidly got back to mild symptom on test of neurological parameter. Also, exogenous melatonin induces both the down-regulation of detrimental genes, such as NOSs and the up-regulation of beneficial gene, including BDNF during long term administration after focal cerebral ischemia. Melatonin treatment reduced the loss of primary motor cortex. Therefore, we suggest that melatonin could be act as prophylactic as well as therapeutic agent for neurorehabilitative intervention.

• 유통과학연구
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• 제14권8호
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• pp.93-100
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• 2016

Purpose - This study examines lenders' reactions to M&A, based on the strength of the lender-borrower relationship and the lender's expectations of the potential benefits or risks of the deal. Research design, data, and methodology - This research addresses the lender's influence on the implementation stage of a large-scale strategic action such as M&A to understand the motivation and dynamics of lenders' responses and empirically examines how the lender-borrower relationship influences the focal firm's merger and acquisition (M&A) transactions, using data on 501 M&A deals in Japan from 1990 to 2010. Results - The presented analysis found that lenders that have a strong lender-borrower relationship, especially those showing a high debt equity ratio, support borrowers' M&A progress and the target firm's lenders resist the deal progressing and may raise the acquisition premium if their current power relative to borrowers is weak. Conclusions - Stakeholders including lenders do not favor strategies of focal firms that threaten their future benefits, while they also tend to estimate the potential benefits and losses by comparing their current circumstances with those of other stakeholders. The empirical results of the presented analysis help explain the mechanism of lenders' reactions and offer insights into the power of a closer and stronger lender-borrower relationship.

• Bulletin of the Korean Chemical Society
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• 제29권4호
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• pp.731-735
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• 2008

The fluid ensemble in the liquid lens, which is composed of electrolyte and silicone oil, is the key material system to vary the focal length processing of the electrochemical desorption. In order to characterize the capability of the liquid lens according to response time and optical range, we prepared a fluid ensemble comprising the electrolyte and oil. To elucidate the physical mechanism of the effective response time, we examined the viscosity dependency while satisfying the requirements for the density and refractive index of the electrolyte and oil, respectively. The characterization results indicated that the response time (up and down) is influenced by the viscosity of the electrolyte and oil. On this basis, we prepared a fluid ensemble capable of reversibly adjusting for the focal length of the liquid lens, as well as the response time. The ensemble is applicable to various systems such as micro-lens and optical sensors.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 춘계학술대회
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• pp.97-99
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• 1996

Intracellular gradients of the free calcium concentration are thought to be critical for the localization of functional responses within a cell. The mechanism of mechanotransduction may be associated with the localized accumulation of calcium stores for shear stress-exposed endothelial cells. The distribution of the calcium stores and the formation of the stress fibers were investigated by the indirect double immunofluorescent staining method with the calreticulin antibody and rhodamine phalloidin under flow condition. The shear stress of steady flow reorganized the cytoskeleton structure including the bundling and translocation to focal contacts. The calcium stores translocated from the cytoplasm to the focal contacting area. Consequently. accumulation of the calcium stores may participate in the shear stress-induced cytoskeleton organization of endothelial cells.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2010년도 학술대회 초록집
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• pp.27-27
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• 2010

There have been several studies about empirical relation between seismic source parameters(e.g., focal mechanisms, depths, magnitudes, etc.) and T-wave observation. In order to delineate the relation, numerical and theoretical approaches to figure out T-wave excitation mechanism are required. In an attempt to investigate source radiation and wave scattering effects in the oceanic crust on T-wave envelopes, we perform three-dimensional numerical modeling to synthesize T-wave envelopes. We first calculate seismic P- and SV-wave energy on the seafloor using the Direct Simulation Monte Carlo based on the Radiative Transfer Theory, which enables us to take into account both realistic seismic source parameters and wave scattering in heterogeneous media, and then estimate excited T-wave energy by normal mode computation. The numerical simulation has been carried out considering the following different conditions: source types (strike and normal faults), source depths (shallow and deep), and wave propagation through homogeneous and heterogeneous Earth media. From the results of numerical modeling, we confirmed that T-wave envelopes vary according to spatial seismic energy distributions on the seafloor for the various input parameters. Furthermore, the synthesized T-wave envelopes show directional patterns due to anisotropic source radiation, and the slope change of T-wave envelopes caused by focal depth. Seismic wave scattering in the oceanic crust is likely to control the shape of envelopes.

• 지질공학
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• 제4권1호
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• pp.29-42
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• 1994

한반도 남부의 순수 대륙을 통과한 지진파를 가지고 미소지진과 지하 인공 폭발의 구별에 관한 연구를 한다.지하 인공 폭발과 미소지진긔 파형 특성은 관측 및 이론 지진 기록지를 가지고 수행한다. 이러한 지지파 파형 특성은 주로 진원메키니즘이 전파 과정보다 강한 영향을 미친다. 미소지진에서는 double-couple진원,그리고 폭발에서는 single-couple진원이 주어진다. 진원이 아주 천부층에 있을때 Rayleigh파의 fundamental mode(1-6초 주기)인 $R_g$파와 guide파인 $L_g$파(8-12초 주기)가 장 나타난다. 또한 폭발에서 SH파는 nodal line이 없기 때문에 초동 진폭이 약하게 나타나을 알 수있다. 인공폭발의 이론 지진 기록지는 압축 초동의 P파와 $R_g{\;}및{\;}L_g$파가 나타남을 알 수있다. 그 반면에 전자의 초동은 관측점에 따라 압축(compression)과 팽창 (dilatation)이 나타났고 Lg파는 복잡하고 $R_g$파는 깊이가 커짐에 따라 사라지는 것을 알 수 있다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.372-375
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• 2006

The MSC is a high resolution multi-spectral camera system which is mounted on the KOMPSAT-II satellite. The electro-optic camera system has a refocusing mechanism which can be used in-orbit by ground commands. By adjusting locations of some elements in optics, the system can be focused precisely. The focus mechanism in MSC is implemented with stepper motor and potentiometer. By reading the value of the potentiometer, rough position of the motor can be understood. The exact location of the motor can not be acquired because the information from the potentiometer can not be so accurate. However, before and after certain events of the satellite, like a satellite launch, the direction of the movement or order of the magnitude of the movement can be understood. In this paper, the trend analysis of the focus motor position during the ground test phase is introduced. This result can be used as basic information for the focus calibration after launch. By studying the long term trend, deviation from the best focal point can be understood. The positions of the focus motors after launch are also compared.