• 제목/요약/키워드: Fluorescent substances

검색결과 45건 처리시간 0.025초

광유발 전자전이를 이용한 유해물질 인식 형광 화학센서의 개발 (Photo-induced Electron Transfer(PET) Based Luminescent Chemosensors Detecting Hazardous Substances)

  • 윤성호
    • 한국전기전자재료학회논문지
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    • 제25권9호
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    • pp.711-716
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    • 2012
  • Based on both organic synthesis and theoretical calculations on the effects of molecular orbital energy levels of amines on the fluorescence properties of the fluorophore, fluorescent "turn-on" chemosensors detecting hazardous substances, including aldehyde chemicals and $Hg^{2+}$ ion, were developed.

가잠조성난의 형광물질과 유리아미노산의 변화 (Changes of Fluorescent Substances and Free Amino Acids in Formed Eggs of the Silkworm(Bombyx mori))

  • 성수일;박광의;김주읍
    • 한국잠사곤충학회지
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    • 제20권1호
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    • pp.11-14
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    • 1978
  • 가잠에 있어서 식도하신경구의 적출이 잠아내의 조성난내의 아미노산 및 자외선형광물질의 변화에 미치는 영향을 Paper Chromatography법에 의해 조사하여 다음과 같은 결과를 얻었다. 1) 잠아의 조성난내에 존재하는 유리아미노산의 종류나 양이 SG적출구와 대조구사이에 차이를 나타내지 않았다. 2) 형광물질인 Riboflavine이 SG적출구인 나방의 조성난내에는 대조구보다 적었다. 3) Rf식 0.06을 나타낸 주홍색의 형광물질은 SG를 적출한 나방의 조성난내에서는 전혀 검출되지 않았으나 대조구에서는 검출되었다. 4) 원점에 나타난 청색의 형광물질은 SG를 적출한 나방의 조성난내에서만 검출되었으나 대조구에서는 검출되지 않았다.

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종자 누출물질을 이용한 노화종자의 선별 (Sorting of Aged Seeds Using Leakage Substances of Crop Seeds)

  • 이동진;임무상;주용하;정길웅;김봉구
    • 한국작물학회지
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    • 제42권6호
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    • pp.783-789
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    • 1997
  • 본 실험은 노화종자를 비파괴적인 방법으로 선별하여 종자의 품질을 증진시키기 위한 것으로 주요 작물종자의 형광 누출을 확인하여 노화종자 선별기술개발 및 아미노산과 당 등의 누출물을 흡착, 시약에 의해 발색시켜 유색종자, 즉 노화종자를 선별하는 기술을 개발하고자 수행하였다. 1. 노화종자에서 형광물질 누출이 많은 작물은 배추, 무, 브로콜리, 콜리플라워, 유채, 박, 쑥갓, 범부채 등이었으며, 이외에도 28종 등 총 36종에서 누출물질을 확인하였다. 2. 배추 종자는 노화처리 기간이 무처리에서 8일까지 길어질수록 무형 광 종자 비율은 91.3%에서 1.7%로 저하되었으며 형광종자 비율은 8.7%에서 98.3%로 증가되었다. 3. 배추의 노화종자에서 sinapine이 누출되는 특성을 이용하여 종자를 수분침지 후 cellulose와 석회분말로 pelleting하여 누출물질을 흡착시킨 후 자외선 광하에서 형광종자(저활력 종자 혹은 노화 종자) 및 무형광 종자(건전종자)로 판별이 가능하였다. 4. 아미노산과 당 등 누출물질의 최적 발색조건은 0.5~l.0% 이상의 ninhydrin 용액과 sodium thiosulfate 용액을 살포 처리한 후 35$^{\circ}C$에서 30분~1시간 처리하는 것이었으며 이 방법으로 유색종자를 만들어 노화종자를 선별할 수 있었다.

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무수은 면광원 램프용 Scanning Backlight 구동회로 (Scanning Backlight Driver for Mercury Free Flat Fluorescent Lamp)

  • 오은태;정용민;이경인;유호원;이준영
    • 전력전자학회논문지
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    • 제14권1호
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    • pp.8-14
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    • 2009
  • 현재 사용하고 있는 LCD(Liquid Crystal Display) Backlight용 램프는 주로 CCFL(Cold Cathod Fluorescent Lamp)과 EEFL(External Electrode Fluorescent Lamp)이 사용되고 있다. 하지만 이 램프는 수은을 사용하여 RoHS(the Restriction of e use of certain Hazardous Substances in electrical and electronic equipment)규제가 점점 강화되면서 사용이 제한되고 있으며, 이에 따라 수은을 사용하지 않는 램프의 제작이 불가피해지고 있다. 또한 LCD TV는 CRT와 PDP와는 다른 LCD만의 Hold-type 특성과 LC(Liquid Crystal)의 응답속도로 인하여 Motion Blur현상이 나타나는 단점이 있다. 본 논문에서는 RoHS 규제를 만족시키는 무수은 면광원 램프를 구동하기 위한 인버터를 제안한다. 제안한 인버터는 포워드 방식을 사용하여 반도체 소자 및 자기 소자의 수를 줄여서 구동회로가 간단하다. 또한 면광원 램프를 세로방향으로 6블록으로 나누어 스캔구동을 하여 Motion Blur현상을 저감 시켰다. 끝으로 실험을 통하여 제안한 인버터의 유용성을 입증하였다.

Suppressive Effects of Coumarins on Pumpkin Seedling Growth and Glutathione S-Transferase Activity

  • Hossain, Md. Daud;Li, Jing;Guo, Shirong;Fujita, Masayuki
    • Journal of Crop Science and Biotechnology
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    • 제11권3호
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    • pp.187-192
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    • 2008
  • The effects of some coumarins(coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin(Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases(GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene(CDNB) and at a concentration of 22 ${\mu}M$. Both ethylacetae(EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity.

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해양 환경의 형광용존유기물에 관한 국내 연구 동향 (Domestic Research Trends on Fluorescent Dissolved Organic Matter in Marine Environment)

  • 김정현
    • Ocean and Polar Research
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    • 제43권4호
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    • pp.353-363
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    • 2021
  • Fluorescent dissolved organic matter (FDOM) is referred to organic matter which absorbs efficiently solar radiation energy and fluorescence in the water column. The component and molecular structure of marine organic matter can be changed depending on the various substances and origins of organic matter, and then the organic matter has unique fluorescent properties. As the cutting-edge analytical techniques of optical measurement continuously developing from last few decades, a study on FDOM has been applied as a biogeochemical tracer to quantify the organic matter concentration and to investigate the behaviors and origins of organic matter. Especially, the marine environment around the Korean Peninsula is an ideal research area to study FDOM because of various oceanographic characteristics and the origins of organic matter. This study describes the general properties of FDOM and introduces the cycling and behaviors of marine organic matter based on the domestic research studies.

Effect of Light-Emitting Diodes on Cordycepin Production in Submerged Culture of Paecilomyces japonica

  • HA, Si Young;JUNG, Ji Young;YANG, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • 제48권4호
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    • pp.548-561
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    • 2020
  • Paecilomyces japonica is widely cultured to produce mycelium for medicinal and health food use. Illumination is an important factor in the growth and production of mycelium in submerged culture. The effects of different light-emitting diode (LED) combinations on the growth and cordycepin content as bioactive substances of mycelium were investigated. The results showed that the mycelium dry weights were lower under dark condition and red LED treatments. Dark condition, fluorescent light, and ultraviolet-A failed to increase the cordycepin content. Blue light was necessary to increase the cordycepin content, and a red-to-blue ratio of 3:7 induced the highest cordycepin content. The cordycepin contents of mycelium in submerged culture were significantly higher in a 12 h/day illumination time under red and blue (red-to-blue ratio of 3:7) LED treatments, showing an increase of up to 38% compared with those under the fluorescent-light control condition. The results demonstrated the roles of light with different wavelengths on the biosynthesis of cordycepin as bioactive substances. The low-heat release and replacement of traditional fluorescent lights with low-energy-consuming LEDs could increase the contents of bioactive substances. After optimization of the cordycepin production using response surface methodology (Box-Behnken design) to its canonical form, the optimum combination was found to be as follows: illumination time = 17.7 h/day, sugar content in the medium = 9.7 g/50 mL, and incubation time = 61.2 h. The model predicted a maximum response of 3779.2 ㎍/mL cordycepin yield.

pH 검출을 위한 미세유동 폴리디아세틸렌 센서칩 개발 (Development of Microfluidic Polydiacetylene Sensor Chip for pH detection)

  • 황현진;송시몬
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2008년도 추계학술대회B
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    • pp.2415-2418
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    • 2008
  • Polydiacetylenes (PDAs) are very attractive chemical substances which have distinctive features of color change and fluorescence emission by thermal or chemical stress. Especially, when PDAs contact with solutions of a particular pH, such as a strong alkaline sodium hydroxide (NaOH) solution or a strong acidic hydrogen chloride (HCl) solution, PDAs change their color from non-fluorescent blue to fluorescent red. In this study, we propose a novel method to detect alkaline pH using PDAs and NaOH solutions by hydrodynamic focusing on a microfluidic chip. Preliminary results indicate that the fluorescent intensity of PDAs increases in respond to the NaOH solution concentrations. Also, the fluorescence is quenched back when the PDAs are in contact with a HCl solution. These results are useful in a microfluidic PDA sensor chip design for pH detection.

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TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구 (The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals)

  • 한양일;김광호;오영복
    • 한국환경보건학회지
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    • 제5권1호
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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연부(軟腐)고구마의 생화학적(生化學的) 연구(硏究) (Biochemical Study on Soft-rotted Sweetpotatoes)

  • 전재근
    • Applied Biological Chemistry
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    • 제10권
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    • pp.47-61
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    • 1968
  • Rhizopus nigricans에 감염(感染)된 고구마의 생화학적(生化學的) 성질(性質)을 구명(究明)하기 위하여 연부(軟腐)고구마로부터 이상대사산물(異常代謝産物)을 분리(分離)하고 그 성질(性質)에 관(關)하여 실험(實驗)한 결과(結果)를 다음과 같이 요약(要約)한다. 1. 건전(健全)한 고구마에는 없는 이상대사산물(異常代謝産物)로서 19개(個)의 Ehrlich's 시약(試藥)에 대(對)한 양성반응성질(陽性反應性質)과 3개(個)의 형광성물질(螢光性物質)의 존재(存在)를 박층(薄層)크로마토그라프로 확인(確認)하였다. 2. 이상대사산물중(異常代謝産物中)에는 그 생성량(生成量)이 가장 많은 물질(物質)로서 Ipomeamarone과 Umbelliferone을 분리(分離)하고 이들의 정제(精製)와 동정(同定)을 행(行)하였다. 3. Ipomeamarone과 Umbelliferone의 경시적(經時的) 생성량(生成量)을 관찰(觀察)하고 이들의 가능(可能)한 생합성(生合成) 경로(經路)에 관(關)하여 고찰(考察)하였다. 4. 연부(軟腐)고구마의 이상대사산물중(異常代謝産物中) Ipomeamarone과 이의 유사물질(類似物質)은 ${\alpha}-amylase$에 대(對)한 활성(活性)을 저해(抵害)하였으며 그 저해형(抵害型)은 비경쟁적(非競爭的)이었다. 5. 연부(軟腐)고구마로부터 분리한 Ipomeamarone은 Yeast의 호흡(呼吸)을 촉진(促進)시켰으며 산화적(酸化的) 인산화반응(燐酸化反應)의 연합해제제(聯合解除劑)인 2, 4-dinitrophenol과 동일(同一)한 효과(效果)를 나타내었다.

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