• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 추계학술대회 논문집 전기물성,응용부문
• /
• pp.89-91
• /
• 2003

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, the improvement of DNA detection system is very important for the determination of this hybridization reaction. In this study, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. First, we have fabricated DNA chip microarray. The particles which were immobilized oligonucleotides were arranged by the random fluidic self-assembly on the pattern chips, using hydrophobic interaction. Second, we have detected DNA hybridization reaction using evanescent field microscopy. The 5'-biotinylated probe oligonucleotides were immobilized on the surface of DNA chip microarray and the hybridization reaction with the Rhodamine conjugated target oligonucleotide was excited fluorescence generated on the evanescent field microscopy. In the foundation of this result, we could be employed as the basis of a probe olidonucleotide, capable of detecting the target oligonucleotide and monitoring it in a large analyte concentration range and various mismatching condition.

• 한국추진공학회지
• /
• 제11권4호
• /
• pp.1-9
• /
• 2007

많은 산업체들은 현재 연소기의 열효율을 증가시키기 위해 노력하고 있으며, 증가되는 NOx 발생으로 환경적인 규제에 직면하고 있다. 우리는 이렇게 열효율 증가와 NOx 발생 감소를 동시에 해결할 수 있도록 새로운 방법을 연구하였다. 이 새로운 개념은 확실히 NOx와 같은 오염물질을 절감시키며, 동시에 반경방향 열전달을 증가시키는 것으로 알려져 있고 이를 세차제트 연소기라고 부른다. 이러한 세차제트 노즐의 특징은 가스터빈의 연소효율의 증가로 이어질 수 있을 것이다. 기본적인 세차제트 노즐에 대한 특설파악을 위해 FLUENT를 이용한 수치해석, 레이저를 이용한 가시화 시험을 진행하였고, 노즐단면에서의 속도분포를 다른 논문의 실험결과와 비교하였다. Centerbody가 있는 경우 세차현상이 잘 일어났고 노즐 출구에서의 확산각이 증가함을 확인하였다.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2011년도 제37회 추계학술대회논문집
• /
• pp.404-407
• /
• 2011

노즐 출구에서 유출되는 이차제트의 코안다 효과를 이용하는 유체역학적 추력편향제어 방법은 초음속 제트의 효율적인 추력편향을 위한 새로운 방법이다. 그동안 진행된 유동가시화 결과에서 관련 기술의 장단점이 관찰된 바 있으나 그 결과가 정성적인 한계가 있었다. 따라서 이차제트의 코안다 효과를 이용한 추력편향제어의 성능특성에 관한 정량적 관찰 연구가 진행되었으며, 이를 통한 시험장치 설계, 보정 및 자료획득 연구결과가 제시되었다.

• 한국산학기술학회:학술대회논문집
• /
• 한국산학기술학회 2011년도 추계학술논문집 2부
• /
• pp.514-517
• /
• 2011

본 연구에서는 사각형태 화장품 케이스 사출성형 시 발생되었던 수지 미성형 불량을 해결하기 위하여 사출성형해석 기술을 통해 성형품 형상변경 및 사출금형 내 유동 시스템 수정방안 도출을 수행하였다. 대상제품인 사각형태 화장품 케이스는 상측부 케이스로서 케이스 외관에 게이트 및 취출흔적이 남지 않아야 함에 따라 측면부 게이트 적용으로 유동거리가 길어져 미성형 불량이 다수 발생하는 제품이다. 따라서, 수지 유동성 향상과 더불어 효율적인 보압전달을 통하여 수지 충전 및 변형발생 저감을 위하여 성형품 형상변화 및 유동시스템 변화에 따른 사출성형해석을 수행하고 결과를 고찰하였다. 이로써 최종적으로 수지 미충전으로 인한 미성형 불량을 제거하고 성형품 변형을 줄일 수 있는 수지유동시스템 수정방안을 제시하였다.

• Nuclear Engineering and Technology
• /
• 제52권3호
• /
• pp.589-596
• /
• 2020

Many components in the assembly section of Sodium-cooled Fast Reactor are made of good corrosionresistant 316 LN Stainless Steel material. To avoid self-welding of the components with the coolant sodium at elevated temperature, hardfacing is inevitable. Ni-based colmonoy-5 is used for hardfacing due to its lower dose rate by Plasma Transferred Arc process due to its low dilution. Since Ni-Cr-B-Si alloy becomes very fluidic while depositing, the major height of the weld overlay rests inside the groove. Hardfacing is also done over the plain surface where grooving is not possible. Therefore, grooved and ungrooved hardfaced specimens were prepared at different travel speeds. Fe content at every 100 ㎛ of the weld overlay was studied by Energy Dispersive Spectroscopy and also the micro hardness was determined at those locations. A correlation between iron dilution from the base metal and the micro hardness was established. Therefore, if the Fe content of the weld overlay is known, the hardness at that location can be obtained using the correlation and vice-versa. A new correlation between micro hardness and dilution coefficient is obtained at different locations. A comparative study between those specimens is carried out to recommend the optimum travel speed for lower dilution.

• KSBB Journal
• /
• 제32권2호
• /
• pp.160-167
• /
• 2017

Coating of individual cells with organic or inorganic materials has drawn a great deal of attention, because it provides the cells with physicochemical durability, which would contribute to the development of bioreactors, biosensor, and lab-on-a-chip, as well as to the fundamental studies in single cell-based biology. Although many strategies have been developed for coating of microbial cells, limited methods are available to coat mammalian cells because most mammalian cells do not have a robust membrane or exoskeleton. Instead, they are enclosed in a lipid bilayer, which is fluidic and vulnerable to changes in its environments. It is more difficult to treat mammalian cells in vitro than microbial cells because the surfaces of mammalian cells are not protected or reinforced by a tough coat. In this work, we report a cytocompatible and degradable nanocoat for mammalian cells. Three types of mammalian cells (HeLa cells, NIH 3T3 fibroblasts, and Jurkat T cells) were individually coated within metal-polyphenol. To maintain the viability of the mammalian cells, we performed the whole processes under strictly physiological culture conditions, and carefully selected nontoxic materials.

• 대한조선학회논문집
• /
• 제47권4호
• /
• pp.525-532
• /
• 2010

In this paper, wave breakers which occur in two dimensional coasts are simulated using a SPH(Smoothed Particle Hydrodynamics) method which represents the movement of fluidic physical volume with particles. As continuative fluid is approximated to the particles, the simulations are performed using fully Lagrangian method without any grid system. Two-dimensional Navier-Stokes equations and continuity equation are used for the numerical simulations. To generate incident waves, a piston type wavemaker is employed. The accuracy of the wave which is numerically generated by the wavemaker is verified by comparing with analytical results. The computations are carried out with various wave heights and slopes. The wave patterns generated through the numerical simulations are compared with several existing experimental and computational results. Agreement between the experimental data and the computation results is comparatively good. Also, the breaker depth index and the breaker height index from the present calculations are compared with the existing experimental results, and the tendency is very similar.

• 센서학회지
• /
• 제13권2호
• /
• pp.85-89
• /
• 2004

This paper represents the development of high sensitivity photo-sensor for the fluorescence detection in the integrated biological analysis system. The finger-type PIN photodiodes were fabricated as the photo-sensor, and had a high sensitivity ($I_{light}/I_{dark}$ = 8720). The interference filter consisted of $TiO_{2}$ and $SiO_{2}$ was directly deposited on the photodiodes. Deposited filter with 95.5% reflection under 532 nm and 98% transmission over 580 nm exceedingly decreased the magnitude of background signal in the detection. The PDMS micro-fluidic channels are bonded on the photodiode by $O_{2}$ plasma treatment. The detection current was proportional to two primary parameters (light intensity, concentration), and the on-chip detection system could detect fluorescence signals down to 100 nM concentration (LOD = Limit of detection of rhodamine).

• 한국가시화정보학회지
• /
• 제12권2호
• /
• pp.18-22
• /
• 2014

Caenorhabditis elegans (C. elegans) is an undulatory nematode which exhibits two distinct locomotion types of swimming and crawling. Although in its natural habitat C. elegans lives in a non-Newtonian fluidic environment, our current understanding has been limited to the behavior of C. elegans in a simple Newtonian fluid. Here, we present some experimental results on the penetrating behavior of C. elegans at the interface from liquid to solid environment. Once C. elegans, which otherwise swims freely in a liquid, makes a contact to the solid gel boundary, it begins to penetrate vertically to the surface by changing its stroke motion characterized by a stiffer body shape and a slow stroke frequency. The particle image velocimetry (PIV) analysis reveals the flow streamlines produced by the stroke of worm. For the worm that crawls on a solid surface, we utilize a technique of traction force microscopy (TFM) to find that the crawling nematode forms localized force islands along the body where makes direct contacts to the gel surface.

• 한국마이크로전자및패키징학회:학술대회논문집
• /
• 한국마이크로전자및패키징학회 2000년도 Proceedings of 5th International Joint Symposium on Microeletronics and Packaging
• /
• pp.29-33
• /
• 2000

MEMS, Micro Electro-Mechanical Systems, present one of the greatest advanced packaging challenges of the next decade. Historically hybrid technology, generally thick film, provided sensors and actuators while integrated circuit technologies provided the microelectronics for interpretation and control of the sensor input and actuator output. Brought together in MEMS these technical fields create new opportunities for miniaturization and performance. Integrated circuit processing technologies combined with hybrid design systems yield innovative sensors and actuators for a variety of applications from single crystal silicon wafers. MEMS packages, far more simple in principle than today's electronic packages, provide only physical protection to the devices they house. However, they cannot interfere with the function of the devices and often must actually facilitate the performance of the device. For example, a pressure transducer may need to be open to atmospheric pressure on one side of the detector yet protected from contamination and blockage. Similarly, an optical device requires protection from contamination without optical attenuation or distortion being introduced. Despite impediments such as package standardization and complexity, MEMS markets expect to double by 2003 to more than ＄9 billion, largely driven by micro-fluidic applications in the medical arena. Like the semiconductor industry before it. MEMS present many diverse demands on the advanced packaging engineering community. With focused effort, particularly on standards and packaging process efficiency. MEMS may offer the greatest opportunity for technical advancement as well as profitability in advanced packaging in the first decade of the 21st century! This paper explores MEMS packaging opportunities and reviews specific technical challenges to be met.