• International Journal of Oral Biology
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• v.33 no.4
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• pp.179-186
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• 2008

Several lines of evidence suggest that osteocytes play a critical role in bone remodeling. Both healthy and apoptotic osteocytes can send signals to other bone surface cells such as osteoblasts, osteoclasts, osteoclast precursors, and bone lining cells through canalicular networks. Osteocytes responding to mechanical strain may also send signals to other cells. To determine the role for osteocytes an mechanical strain in bone remodeling, we examined the effects of fluid flow shear stress on osteoclast precursor cell and osteoblast proliferation and recruitment induced by osteocytes. In addition, the effects of fluid flow shear stress on osteocyte M-CSF, RANKL, and OPG mRNA expression were also examined. MLO-Y4 cells were used as an in vitro model for osteocytes, RAW 264.7 cells and MOCP-5 cells as osteoclast precursors, and 2T3 cells as osteoblasts. MLO-Y4 cells conditioned medium (Y4-CM) was collected after 24h culture. For fluid flow experiments, MLO-Y4 cells were exposed to 2h of pulsatile fluid flow (PFF) at 2, 4, 8, $16{\pm}0.6\;dynes/cm^2$ using the Flexcell $Streamer^{TM}$ system. For proliferation assays, MOCP-5, RAW 264.7, and 2T3 cells were cultured with control media or 10-100% Y4 CM. Cells were cultured for 3d, and then cells were counted. RAW 264.7 and 2T3 cell migration was assayed using transwells with control media or 10-100% Y4-CM. M-CSF, RANKL and OPG in MLO-Y4 mRNA expression was determined by semiquantitative RT-PCR. Y4-CM increased osteoclast precursor proliferation and migration, but decreased 2T3 cell proliferation and migration. CM from MLO-Y4 cells exposed to PFF caused decreased RAW 267.4 cell proliferation and migration and 2T3 migration compared to control Y4-CM. However, Y4-CM from cells exposed to PFF had no effect on 2T3 osteoblastic cell proliferation. PFF decreased RNAKL mRNA and increased OPG mRNA in MLO-Y4 cells compared to control(without PFF). PFF had no effect on M-CSF mRNA expression in MLO-Y4 cells. These results suggest that osteocytes can regulate bone remodeling by communication with osteoclast precursors and osteoblasts and that osteocytes can communicate mechanical signals to other cells.

• Journal of Ocean Engineering and Technology
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• v.12 no.2 s.28
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• pp.79-86
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• 1998

A three-dimensional WIG (Wing In Ground effect) moving above free surface is numerically studied by means of finite difference techniques. The air flow field around the WIG is analyzed by MAC (Marker & Cell) method, and interactions between WIG and the free surface are appeared as the variation of pressure distribution acting on the free surface. To analyze the wavemaking phenomena by those pressure distributions, the NS (Navier-Stokes) solver is employed in which nonlinearities of the free surface conditions can be included. Through the numerical simulation, Cp values and lift/drag ratio are carefully reviewed by changing the height/chord ratio. The section shape of model is NACA0012 with the span/chord ratio of 3.0. Through computational results, it is confirmed that the effect of free surface is small enough to treat it as a rigid wavy wall.

• International Journal of Naval Architecture and Ocean Engineering
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• v.7 no.6
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• pp.1034-1043
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• 2015

This is the continuation of a numerical study on vortex shedding from a blunt trailing-edge of a hydrofoil. In our previous work (Lee et al., 2015), numerical schemes for efficient computations were successfully implemented; i.e. multiple domains, the approximation of domain boundary conditions using cubic spline functions, and particle-based domain decomposition for better load balancing. In this study, numerical results through a hybrid particle-mesh method which adopts the Vortex-In-Cell (VIC) method and the Brinkman penalization model are further rigorously validated through comparison to experimental data at the Reynolds number of $2{\times}10^6$. The effects of changes in numerical parameters are also explored herein. We find that the present numerical method enables us to reasonably simulate vortex shedding phenomenon, as well as turbulent wakes of a hydrofoil.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3073-3076
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• 2012

Terpinen-4-ol is a terpene found in the rhizome of Plai (Zingiber montanum (Koenig) Link ex Dietr.). In this study apoptogenic activity and mechanisms of cell death induced by terpinen-4-ol were investigated in the human leukemic MOLT-4 cell line. Terpinen-4-ol exhibited cytotoxicity in MOLT-4 cells, with characteristic morphological features of apoptosis by Wright's staining. The mode of cell death was confirmed to be apoptosis by flow cytometric analysis after staining with annexin V-FITC and propidium iodide. A sub-G1 peak in DNA histograms of cell cycle assays was observed. Terpinen-4-ol induced-MOLT-4 cell apoptosis mediated through an intrinsic pathway involving the loss of mitochondrial transmembrane potential (MTP) and release of cytochrome c into the cytosol. In addition, terpinen-4-ol also induced apoptosis via an extrinsic pathway by caspase-8 activation resulting in the cleavage of cytosolic Bid. Truncated-Bid (tBid) translocated to mitochondria and activated the mitochondrial pathway in conjunction with down-regulation of Bcl-2 protein expression. Caspase-3 activity also increased. In conclusion, terpinen-4-ol can induce human leukemic MOLT-4 cell apoptosis via both intrinsic and extrinsic pathways.

• Applied Chemistry for Engineering
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• v.32 no.6
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• pp.613-620
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• 2021

A flow channel shape of PEMFC has an influence on the internal flow uniformity. If the reactant distribution in a flow path is not uniform during operation, both catalyst deactivation and mechanical damage of membrane could occur resulting in decreasing the membrane electrode assembly (MEA) durability. Numerous studies concerning flow design have been conducted to make smooth supply and uniform distribution of reactants in fuel cells. The baffle of flow path could improve fuel cell performance through the forced convection effect. A sub-channel, as an additional air flow path, could increase the reactant concentration and reduce the mass transfer loss via a smooth water discharge. In this study, computational fluid dynamics (CFD) was used to analyze the effect of blocks and sub-channels on the current density and oxygen concentration of the fuel cell. As a result, the limit current density and oxygen concentration at a rear block increased when using blocks and sub-channels in a flow channel. In particular, the current density increased significantly when the sub-channel was placed between two blocks. Also, the sub-channel position was optimized by analyzing the oxygen concentration, and the oxygen concentration was recovered at a rear block in the fuel cell.

• Membrane Journal
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• v.29 no.5
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• pp.284-291
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• 2019

In this study, for the improvement of flow pattern with the CDI module that had the larger electrodes, it was designed with the rectangular type which is gradually wider from the inlet. Based on this, both the flow pattern of feed solution and dead zone were observed and the internal pressure, streaming line and velocity vector distribution were analyzed through the computational flow dynamics and compared with the experimental results. For all flow rates of 10, 20, 30 mL/min, there were no dead zones and the flow patterns were maintained constant. Therefore, it may be possible that the larger electrodes are applied to the CDI process.

• Journal of Life Science
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• v.21 no.11
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• pp.1573-1578
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• 2011

Milk thistle (silybum marianum) is a famous dietary supplement widely used in the United States and Europe. Silbinin is a major biologically active compound of milk thistle and has strong antioxidant and radical scavenger activities. Anticancer activities, as well as chemopreventive effects on various cancer cell lines, including prostate, lung, colon, skin, and bladder, have also been reported in silbinin. In the present study, we investigated the anticancer effects of silibinin and apoptosis through cell cycle arrest on prostate cancer cell PC-3. We performed cell viability by MTT assay and western blotting to confirm cell cycle check point proteins such as cyclin A/D1/E and cyclin-dependent kinase (CDK) 2/4/6. To quantify silibinin-induced apoptotic cell death of PC-3, Annexin V and PI double staining was performed by flow cytometry, by which its cell distribution was determined. As a result, silibinin inhibited the cell growth of PC-3 cells in a time- and dose-dependent manner, and its treatment resulted in cell cycle arrest at the G1 phase. Also the level of cell cycle check point proteins (cyclin, CDK) was decreased by silibinin in a dose-dependent manner. Taken together, we suggest that apoptosis of prostate cancer cell line PC-3 induced by silibinin is associated with cell cycle arrest through decrease of cell cycle check point proteins, caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage.

• The Korean Journal of Physiology
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• v.28 no.2
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• pp.203-214
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• 1994

Using the methods of stopped-flow and epifluorescence microscopy with entrapped fluorophore, membrane permeability of $NH_3$ and weak acids in liposomes, renal brush border (BBMV) and basolateral membrane vesicles (BLMV), and primary culture cells from renal proximal tubule was measured. Permeability coefficient (cm/sec) of $NH_3$ was $(2.9{\times}10^{-2}$ in phosphatidylcholine liposome $25^{\circ}C)$, $5.9{\times}10^{-2}$ in renal proximal tubule cell $(37^{\circ}C)$, $4.0{\times}10^{-2}\;and\;2.4{\times}10^{-2}$ in BBMV and BLMV $(25^{\circ}C)$, respectively. Formic acid has the highest permeability coefficient among the weak acids tested, which was $4.9{\times}10^{-3}$ in liposome, $5.0{\times}10^{-3}$ in renal proximal tubule cell, $9.1{\times}10^{-3}$ in BBMV and $3.8{\times}10^{-3}$ in BLMV. There was a linear relationship between external concentration of nonionized formic acid and initial rate of flux of formic acid in liposome, and the slope coincided with the value of permeability coefficient of formic acid measured in pH 7.0. These results show that techniques of stopped-flow and epifluorescence microscopy with entrapped fluorophore provide the precise method of measurement of very rapid transport of nonelectrolytes through membranes with the advantages of instantaneous mixing effect, good resolution time and easy manipulation.

• Transactions of the Korean hydrogen and new energy society
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• v.22 no.2
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• pp.191-198
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• 2011

According to the propagation of fuel cell system, the importance of that system efficiency is being magnified. Thus, the efficiency improvement of reformer which is the important factor of fuel cell system will be required. This study has been experimentally performed to evaluated the performance of plate type STR reactor. At first, we changed fuel flow rate (2, 3 and 4 l/min) in burner, and then we measured a proportion of hydrogen in produced gas through the STR reactor by G.C for evaluating the performance of plate type STR reactor in various fuel supply conditions. And we changed S/C ratio (2 and 4) and measured a proportion of hydrogen in produced gas through the STR reactor. As a results, condition at fuel flow rate 2 and 3 l/min could not be supplied amount of heat for STR sufficiently. Condition at fuel flow rate 4 l/min could supplied a heat excessively. And condition at S/C ratio 2, reaction occurred insufficiency. But condition at S/C ratio 4 was excess. From above, we found the optimum conditions that were fuel rate 3.5 l/min and S/C ratio 3.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.1131-1142
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• 2013

This study aims to verify the applicability of TRANSIMS (TRansportion ANalysis SIMulation System) in interrupted traffic flow through calibration and validation process based on observation data; such as headway, traffic volume, speed, and travel time from Dalguberl Boulevard in Dae-gu metropolitan city. On this study, several micro-simulation parameters are derived from the calibration and validation process through performing a headway comparison and applying an ID back tracking methodology. As a result, it is figured out that actual circumstances of Korean roadway; for example, traffic volume per lane, speed, and travel time, can be applied on the TRANSIMS. Especially, it was possible to find out the influence of cell size parameter to traffic flow characteristic of simulation. However, it is hard to conclude that TRANSIMS is applicable to Korean roadway environment with studying particular target area. Therefore, additional studies; such as more case studies with various types of road, signal, and land use, will be required to localize TRANSIMS to Korea.