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Quinetides: diverse posttranslational modified peptides of ribonuclease-like storage protein from Panax quinquefolius as markers for differentiating ginseng species

  • Zhao, Qiang;Bai, Yunpeng;Liu, Dan;Zhao, Nan;Gao, Huiyuan;Zhang, Xiaozhe
    • Journal of Ginseng Research
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    • v.44 no.5
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    • pp.680-689
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    • 2020
  • Background: Peptides have diverse and important physiological roles in plants and are ideal markers for species identification. It is unclear whether there are specific peptides in Panax quinquefolius L. (PQ). The aims of this study were to identify Quinetides, a series of diverse posttranslational modified native peptides of the ribonuclease-like storage protein (ginseng major protein), from PQ to explore novel peptide markers and develop a new method to distinguish PQ from Panax ginseng. Methods: We used different fragmentation modes in the LTQ Orbitrap analysis to identify the enriched Quinetide targets of PQ, and we discovered Quinetide markers of PQ and P. ginseng using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis. These "peptide markers" were validated by simultaneously monitoring Rf and F11 as standard ginsenosides. Results: We discovered 100 Quinetides of PQ with various post-translational modifications (PTMs), including a series of glycopeptides, all of which originated from the protein ginseng major protein. We effectively distinguished PQ from P. ginseng using new "peptide markers." Four unique peptides (Quinetides TP6 and TP7 as markers of PQ and Quinetides TP8 and TP9 as markers of P. ginseng) and their associated glycosylation products were discovered in PQ and P. ginseng. Conclusion: We provide specific information on PQ peptides and propose the clinical application of peptide markers to distinguish PQ from P. ginseng.

Dynamic Object Tracking of a Quad-rotor with Image Processing and an Extended Kalman Filter (영상처리와 확장칼만필터를 이용한 쿼드로터의 동적 물체 추종)

  • Kim, Ki-jung;Yu, Ho-Yun;Lee, Jangmyung
    • Journal of Institute of Control, Robotics and Systems
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    • v.21 no.7
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    • pp.641-647
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    • 2015
  • This paper proposes a new strategy for a quad-rotor to track a moving object efficiently by using image processing and an extended Kalman filter. The goal of path planning for the quad-rotor is to design an optimal path from the start point to the destination point. To lengthen the freight time of the quad-rotor, an optimal path is required to reduce the energy consumption. To track a moving object, the mark signed on the moving object has been detected by a camera mounted first on the quad-rotor. The center coordinates of the mark and its area are calculated through the blob analysis which is one type of image processing. The mark coordinates are utilized to obtain information on the motion direction and the area of the mark is utilized to recognize whether the object moves backward or forward from the camera on the quad-rotor. In addition, an extended Kalman filter has been applied to predict the direction and speed of the dynamically moving object. Through these schemes, it is aimed that the quad-rotor can track the dynamic object efficiently in terms of flight distance and time. Through the two different route freights of the quad-rotor, the performance of the proposed system has been demonstrated.

The Strain Corrections for Accuracy Improvement to Predict Large Deformation of Wings (날개 대변형 예측의 정확성 향상을 위한 변형률 보정)

  • Lee, Hansol;Kim, In-Gul;Park, Sunghyun;Kim, Min-Sung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.1
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    • pp.1-11
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    • 2016
  • The information about the deformations of high-aspect-ratio wings is needed for the real-time monitoring of structural responses. Wing deformation in flight can be predicted by using relationship between the curvatures and the strains on the wing skin. It is also necessary to consider geometric nonlinearity when the large deformation of wing is occurred. The strain distribution on fixed-end is complex in the chordwise direction because of the geometric shape of fixed-wings on fuselages. Hence, the wing displacement can be diversely predicted by the location of the strain sensing lines in the chordwise direction. We conducted a study about prediction method of displacements regardless of the chordwise strain sensing locations. To correct spanwise strains, the ratio of spanwise strain to chordwise strain, Poisson's ratio, and the ratio of the plate strain to the beam strain were used. The predicted displacements using the strain correction were consistent with those calculated by the FEA and verified through the bending testing.

New Carotid Artery Stenosis Measurement Method Using MRA Images (경동맥 MRA 영상을 이용한 새로운 내경 측정 방법)

  • 김도연;박종원
    • Journal of KIISE:Software and Applications
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    • v.30 no.12
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    • pp.1247-1254
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    • 2003
  • Currently. the north american symptomatic carotid endarterectomy trial, european carotid surgery trial, and common carotid method are used to measure the carotid stenosis for determining candidate for carotid endarterectomy using the projection angiography from different modalities such as digital subtraction angiography. rotational angiography, computed tomography angiography and magnetic resonance angiography. A new computerized carotid stenosis measuring system was developed using MR angiography axial image to overcome the drawbacks of conventional carotid stenosis measuring methods, to reduce the variability of inter-observer and intra-observer. The gray-level thresholding is one of the most popular and efficient method for image segmentation. We segmented the carotid artery and lumen from three-dimensional time-of-flight MRA axial image using gray-level thresholding technique. Using the measured intima-media thickness value of common carotid artery for each cases, we separated carotid artery wall from the segmented carotid artery region. After that, the regions of segmented carotid without artery wall were divided into region of blood flow and plaque. The calculation of carotid stenosis degree was performed as the following; carotid stenosis grading is(area measure of plaque/area measure of blood flow region and plaque) * 100%.

Transducer Combination for High-Quality Ultrasound Tomography Based on Speed of Sound Imaging (속도 분포 기반 단층촬영을 위한 최적의 트랜스듀서의 조합)

  • Kim, Young Hun;Park, Kwan Kyu
    • Journal of the Korean Society for Nondestructive Testing
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    • v.36 no.1
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    • pp.27-34
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    • 2016
  • The type of ultrasound transducer used influences the quality of a reconstructed ultrasound image. This study analyzed the effect of transducer type on ultrasound computed tomography (UCT) image quality. The UCT was modeled in an ultrasound simulator by using a 5 cm anatomy model and a ring-shape 5 MHz 128 transducer array, which considered attenuation, refraction, and reflection. Speed-of-sound images were reconstructed by the Radon transform as the UCT image modality. Acoustic impedance images were also reconstructed by the delay-and-sum (DAS) method, which considered the speed of sound information. To determine the optimal combination of transducers in observation, point-source, flat, and focused transducers were tested in combination as trasmitters and receivers; UCT images were constructed from each combination. The combination of point-source/flat transducer as transmitting and receiving devices presented the best reconstructed image quality. In UCT implementation, the combination of a flat transducer for transmitting and a point transducer for receiving permitted acceptable image quality.

MR Angiography with Simultaneous Data Acquisition of Arteries and Veins(SAAV) Method and Artery-Vein Color Mapping in 0.3T MRI System (0.3T MRI 시스템에서의 동.정맥 동시 획득을 위한 자기공명 혈류 영상 기법(SAAV)과 동.정맥 color mapping)

  • 조종운;조지연;서성만;은충기;문치웅
    • Journal of Biomedical Engineering Research
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    • v.24 no.4
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    • pp.275-280
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    • 2003
  • The method of simultaneous data acquisition of arteries and veins(SAAV) was suggested to obtain MR angiography of arteries and veins at 0.3T low filed MRI system (Magfinder, AlLab. Korea). Two separated artery- and vein-images were put together using AVCM(Artery-Vein Color Mapping) algorithm and presented in the same image. In this study, artery- and vein-separated angiograms of volunteer's neck were obtained. Two dimensioal blood-enhanced images wre sequentially obtained using SAAV pulse sequence based on time-of-flight(TOF) method with flow compensation. Imaging parameters were TR/TE=70/12msec. FOV=230mm, slice thickness = 3mm, flip angle=90$^{\circ}$, matrix size=256${\times}$256${\times}$64mm. TSat TH/SPA=15/20mm, Ts_v=10msec and Ts_a=40ms. 3D MRA images were reconstructed using the maximum intensity projection(MIP) and the artery-vein color mapping(AVCM) algorithm. This study showed good possibility of clinical applications of MRA in 0.3T which provides valuable diagnostic information of clinical vascular diseases.

Effects of Customer Satisfaction by Airline e-Services (항공사 e-서비스가 고객 만족도에 미치는 영향)

  • Kim, Yoon-Tae
    • The Journal of the Korea Contents Association
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    • v.9 no.6
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    • pp.357-369
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    • 2009
  • With the development and generalization of internet and information technology, airlines has tried to reduce their business expenses and commissions to travel agencies and enhance service qualities through service automation and simplification, such as internet booking and ticketing, self check-in, in-flight internet and RFID for checked baggage. The statistical techniques conducted for this empirical analysis are frequency analysis, reliability analysis, factor analysis, confirmatory factor analysis and multiple regression analysis. This research has tried to examine factors of airline e-services that influence on recommendation re-purchase intention and satisfaction. Results has found that only on-line reservation and ticketing factor had significant effect for recommendation and re-purchase intention and all e-service factors produced significant effect to total satisfaction. It was also recommend that airlines have to provide easy and more familiar e-service system to their passengers to deliver better services.

Building Roof Reconstruction in Remote Sensing Image using Line Segment Extraction and Grouping (선소의 추출과 그룹화를 이용한 원격탐사영상에서 건물 지붕의 복원)

  • 예철수;전승헌;이호영;이쾌희
    • Korean Journal of Remote Sensing
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    • v.19 no.2
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    • pp.159-169
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    • 2003
  • This paper presents a method for automatic 3-d building reconstruction using high resolution aerial imagery. First, by using edge preserving filtering, noise is eliminated and then images are segmented by watershed algorithm, which preserves location of edge pixels. To extract line segments between control points from boundary of each region, we calculate curvature of each pixel on the boundary and then find the control points. Line segment linking is performed according to direction and length of line segments and the location of line segments is adjusted using gradient magnitudes of all pixels of the line segment. Coplanar grouping and pplygonal patch formation are performed per region by selecting 3-d line segments that are matched using epipolar geometry and flight information. The algorithm has been applied to high resolution aerial images and the results show accurate 3D building reconstruction.

Remote Fault Detection in Conveyor System Using Drone Based on Audio FFT Analysis (드론을 활용하고 음성 FFT분석에 기반을 둔 컨베이어 시스템의 원격 고장 검출)

  • Yeom, Dong-Joo;Lee, Bo-Hee
    • Journal of Convergence for Information Technology
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    • v.9 no.10
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    • pp.101-107
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    • 2019
  • This paper proposes a method for detecting faults in conveyor systems used for transportation of raw materials needed in the thermal power plant and cement industries. A small drone was designed in consideration of the difficulty in accessing the industrial site and the need to use it in wide industrial site. In order to apply the system to the embedded microprocessor, hardware and algorithms considering limited memory and execution time have been proposed. At this time, the failure determination method measures the peak frequency through the measurement, detects the continuity of the high frequency, and performs the failure diagnosis with the high frequency components of noise. The proposed system consists of experimental environment based on the data obtained from the actual thermal power plant, and it is confirmed that the proposed system is useful by conducting virtual environment experiments with the drone designed system. In the future, further research is needed to improve the drone's flight stability and to improve discrimination performance by using more intelligent methods of fault frequency.

Flow Analysis and Flight Experiment for Optimum Height of Weather Data Sensor (기상데이터 센서의 최적 높이를 위한 유동해석 및 비행실험)

  • Kim, Young-in;Ku, SungKwan;Park, ChangHwan
    • Journal of Advanced Navigation Technology
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    • v.22 no.6
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    • pp.551-556
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    • 2018
  • In recent years, drones have been used to measure aircraft flights data and weather information. Related applications include the measure for low-altitude atmospheric data, the measure for atmospheric fine dust, and the measure for air pollution. However, the mounting position of the atmospheric measurement sensor should be mounted by considering the effects of propeller flow, the EMI effects, and the changes in the weight of the drone. Among these, the upper flow of the propeller affects the wind speed and direction, so the optimal position should be selected. This study deals with the proper height of the atmospheric data measurement sensor. Through the flow analysis, we study the flow characteristics of around a drone and suggest the proper sensor mounting height.