• 수산해양기술연구
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• 제50권2호
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• pp.169-175
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• 2014

The age and growth of rabbit fish, Siganus fuscescens were investigated from samples monthly collected in the coast of Jeju island, Korea from February to December 2012. Ages were determined from annuli in otoliths, and annuli were formed in one or two months immediately after spawning once a year. Also, main spawning period was estimated between July and August, thus rings were considered as annual marks. The von Bertalanffy growth equations estimated from a non-linear regression method were $L_t=37.24(1-e^{-0.33(t+1.24)})$ ($R^2=0.92$) for female and $L_t=31.44(1-e^{-0.45(t+1.23)})$ ($R^2=0.90$) for male, and the growth between female and male was different.

• 한국해양바이오학회지
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• 제6권1호
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• pp.17-25
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• 2014

Periodically, harmful algal blooms (HABs) have occurred, with impacts on various areas including public health, tourism, and aquatic ecosystems, especially aquacultured and caged fisheries. To prevent or manage invasions of HABs into fish farms on an emergency basis, many methods have been proposed. Frequently over the past 30 years in coastal countries, treatments of clay and clay mixed with polyaluminum chloride (PAC) and chitosan have been tested for HAB-removal effectiveness in both the laboratory and the field. In Korea, yellow loess clay (hwangto) has been dispersed using electrolytic clay dispensers, both to decrease the amount of yellow loess clay's usage in containers and enhance HAB-removal efficiency. However, this emergency method has limitations, among which is the requirement for more effective controlling agents for field applications. Thus, in this paper, we review technologies for clay-based red tides prevention and control and their limitations, and, further, introduce next-generation algicidal technologies for the emergency protection of fish farms.

• Fisheries and Aquatic Sciences
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• 제7권4호
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• pp.175-183
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• 2004

The dinoflagellate Alexandrium tamiyavanichii Balech, a producer of toxins causing paralytic shellfish poisoning (PSP), has recently been considered as one of main organisms responsible for toxication of shellfish in Japan. In this study, A. tamiyavanichii was subjected to a molecular phylogenetic analysis inferred from 28S rDNA D1-D2 sequences and a species-specific LSU rRNA-targeted oligonucleotide DNA probe was designed to identify A. tamiyavanichii using the whole cell-FISH (fluorescence in situ hybridization). The sequences of the 28S rDNA D1-D2 region of A. tamiyavanichii showed no difference from A. cohorticular AF1746l4 (present name A. tamiyavanichii) and formed a distinct clade from the 'tamarensis species complex'. The probe, TAMID2, reacted specifically with A. tamiyavanichii cultured cells, without any cross-reaction with other species belonging to the same genus, including A. tamarense, A. catenella, A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax. In a test of cross-reactivity with a field sample, TAMID2 reacted consistently with only A. tamiyavanichii, indicating that the present protocol involving the TAMID2 probe might be useful for detecting toxic A. tamiyavanichii in a simple and rapid manner.

• Animal cells and systems
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• 제3권1호
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• pp.1-7
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• 1999

Gonadotropin-releasing hormone (GnRH) was originally isolated as a hypothalamic peptide that regulates reproduction by stimulating the release of gonadotropins. Using comparative animal models has led to the discovery that GnRH has a more ancient evolutionary origin. Durinq evolution GnRH peptide underwent gene duplication and structural changes to give rise to multiple molecular forms of GnRHs. Mammalian GnRH initially considered to be the sole molecular form, is now grouped as a family of peptides along with GnRH variants determined from representatives in all classes of vertebrates. Vertebrate species including primates and humanshave more than one GnRH variant in individual brains; a unique GnRH form in the forebrain and chicken IIGnRH in the midbrain. Furthermore, several species of bony fish have three molecular variants of GnRH: salmon GnRH sea-bream GnRH and chicken II GnRH. Also, it has been shown that in addition to the olfactory placodes and the midbrain, there is a third embryonic source of GnRH neurons from the basal diencephalon in birds and fish, which might be true for other vertebrates. Therefore, comparative animal models like fish with discrete sites of expression of three molecular variants of GnRH in individual brains, could provide insight into novel functions of GnRH variants, conservation of gene regulation, and mechanisms governing reproduction in vertebrates.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.496-502
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• 2006

Off-flavors and unfavorable odors in tuna fish oil were successfully removed and identified using supercritical carbon dioxide extraction, while retaining variable compounds, polyunsaturated fatty acids such as EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid). Samples of oil were extracted in a 100 mL semi-batch stainless steel vessel under conditions which ranged from 8 to 20 MPa and $20\;to\;60^{\circ}C$ with solvent ($CO_{2}$) flows from 10 g/min. GC-MS was used to identify the main volatile components contributing to the off-flavors and odors which included 2-methyl-1-propanol, 2,4-hexadienal, cyclopropane, and octadiene. Analyses of oil extracted at $40^{\circ}C$, 20 MPa showed a 99.8% reduction in dimethyl disulfide. Other significant off-flavors identified were 2-methyl-butene, 3-hydroxy butanal and ethylbenzene.

• 한국식생활문화학회지
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• 제27권1호
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• pp.1-11
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• 2012

This study investigated the effects of a healthy image on the preference and intake frequency of meat and animal products. The study looked into beef, pork, chicken, sausage, mackerel, cutlass fish, croaker, tuna, squid, shrimp, clams, fish cakes, eggs, milk, yogurt, ice cream, and cheese. A total of 359 usable surveys given to elementary school students, college students, and adults were collected using a convenient sampling method. While milk had the healthiest image, sausage had the least healthy image. The respondents preferred yogurt the most and sausage the least. The intake frequency of eggs was the highest and clams the lowest. The healthy image, preference, and intake frequency for all studied foods showed significant differences across both gender and age. The relationship between healthy image and preference was significant for all foods, and a healthy image always had a positive influence on preference. The relationship of healthy image and intake frequency was significant in 14 foods except for mackerel, cutlass fish, and tuna. Also a healthy image created a positive effect on the intake frequency of 14 foods.

• 수산해양기술연구
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• 제46권2호
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• pp.148-156
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• 2010

This study was conducted to collect the information on the behavioral characteristics and the habitat environment of mandarin fish (Siniperca schezeri) and catfish (Parasilurus asitus) with acoustic telemetry method in Chungju Lake, Korea. Mandarin fish tended to stay within 1km from the release points in downstream, and had a strong diurnal behavior. They approached to the lakefront at night. They also preferred to stay at deep water off the lakefront. The average swimming speed was faster at night (0.4BL/s) than during a day (0.2BL/s). They swam the shallow water area at night. Catfish frequently moved between upstream and downstream. Catfish tended to act during a day. The average swimming speed was faster during a day (0.3BL/s) than at night (0.2 BL/s). The average swimming depth was 14.3m, and they tended to float about 4m during a day.

• 해양환경안전학회지
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• 제25권3호
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• pp.373-380
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• 2019

In this paper, a pico hydro turbine employing low head circulation water at fish farms is designed and evaluated. Due to the advantages of simple structures, small head requirements, and low-cost investment, the constant thickness propeller turbine is considered as a feasible solution. The design process based on the free vortex method is presented in full detail, and a 4-blade runner is built using BladeGen. The turbine performance is analyzed both numerically and via experimental methods. Despite slight differences, the results show similar trends between CFD simulations and experiments carried out on factory test-rigs in a wide range of working conditions. At the design flow rate, the turbine achieves the best efficiency of 70 %, generating 3.5 kW power when rotating at 420 rpm. The internal flow field, as well as the turbine's behavior, are investigated through the distribution of blade streamlines, pressure, and velocity around the runner. Moreover, the pressure coefficient on the blade surface at 3 span positions is plotted while the head loss for each simulation domain is calculated and displayed by charts.

• 한국동물생명공학회지
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• 제36권4호
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• pp.169-174
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• 2021

Mutations in the luteinizing hormone/chorionic gonadotropin receptors (LH/CGRs), representatives of the G protein-coupled receptor family, have been rapidly identified over the last 20 years. This review aims to compare and analyze the data reported the activating and inactivating mutations of the LH/CGRs between human, rat, equine and fish, specifically (Japanese eel Anguilla japonica). Insights obtained through detailed study of these naturally-occurring mutations provide a further update of structure-function relationship of these receptors. Specifically, we present a variety of data on eel LH/CGR. These results provide important information about LH/CGR function in fish and the regulation of mutations of the highly conserved amino acids in glycoprotein hormone receptors.

• Fisheries and Aquatic Sciences
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• 제25권1호
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• pp.31-39
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• 2022

To clarify the factors influencing the physical properties of fish after heat treatments, we investigated changes in the properties of proteins in the dorsal ordinary and dark muscle of yellowtail (Seriola quinqueradiata) heated under different conditions commonly used for the purposes of food hygiene. High-temperature/short-time heating (85℃ for 90 s and 75℃ for 60 s) affected the protein solubility more than low-temperature/long-time heating (63℃ for 30 min). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and differential scanning calorimetry showed that low-temperature/long-time heating reduced the degree of actin denaturation in fish compared with that by other heating conditions. In addition, collagen solubility was enhanced with low-temperature/long-time heating. Therefore, these results suggest that differences in the degree of actin and collagen denaturation are responsible for the enhanced meat tenderness and diminished meat shrinkage, resulting from low-temperature/long-time heating.