• 한국어병학회지
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• 제32권2호
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• pp.81-89
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• 2019

As an epidemiological survey, mortality of marbled rockfish, Sebastiscus tertius observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues (gill, liver, spleen and kidney). Bacteria was isolated from spleen and kidney in moribund and mortality fish. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida using API 20E and 20NE, API 50CH API ZYM system. Under light microscopy, infected marbled rockfish showed the lifting of the lamella epidermal layer, edematous changes and hypertrophy of epithelial cell in the gill filament. The atrophy of the mucosal fold, erythema in the intestine, and the necrosis of hematopoietic tissue and renal tubule cells with karyolysis were observed in the kidney. In this study was demonstrated the histological reaction of red marbled rockfish infected by Aeromonas salmonicida. Furthermore, this is the first account of extensive dermatitis in Sebastiscus tertius due to atypical A. salmonicida infection, which has high potential in aquaculture among native fish species.

• 한국어병학회지
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• 제32권2호
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• pp.113-121
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• 2019

Effects on glutathione-related antioxidant parameters were examined after a chronic exposure of olive flounder, Paralichthys olivaceus to dietary 4-tert-octylphenol (4-tert-OP). Fish were fed diets containing 4-tert-OP at 0, 1, 5 and 10 mg/kg diet for 6 weeks. Antioxidant parameters examined were reduced glutathione (GSH) contents and enzyme activities of glutathione reductase (GR), glutathione S-transferase (GST) and glutathione peroxidase (GPx) in tissue homogenates of the liver, kidney and gill. It was observed that all parameters examined increased although there were some differences in dose responses and temporal patterns in the increase. GSH contents increased after exposure to 4-tert-OP in the three organs examined. However, the GSH increase was evident only after 4 weeks in the liver whereas it was elevated after 2 weeks in the kidney and gill. GR activity exhibited a significant increase in response to 4-tert-OP at 1 mg/kg in all three organs, however, its activity returned to control levels when exposed to 5 and 10 mg/kg. Hepatic GST activity showed an earlier increase at week 2 in contrast to the kidney and gill where they increased after 4 weeks of 4-tert-OP exposure. Temporal patterns in GPx activity changes to 4-tert-OP exposure were dissimilar among the organs: hepatic activity increased from week 2 through week 6; renal activity increased transiently at week 2; gill levels were higher through weeks 4 - 6. The results suggest that elevation of several GSH-related antioxidant parameters can be considered as evaluation criteria for 4-tert-OP-induced oxidative stress in a fish.

• 한국어병학회지
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• 제14권2호
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• pp.81-90
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• 2001

구리에 대한 넙치의 조직학적 반응을 알아보기 위하여 아가미, 간췌장 및 신장조직상의 변화를 조사하였다. 대조구에서는 5주 후 아가미에서 점액세포와 염세포의 증가가 관찰되었다. 간췌장과 신장에서는 혈구의 증가와 일부 흑색 대식세포들이 관찰되었다. 구리 노출구 가운데 조직학적 변화가 처음 관찰된 농도구는 0.05mg/$\ell$/7d 였으며, 아가미에서는 염세포의 증가, 간췌장에서는 간세포의 활성화와 췌장 효소원과립의 감소, 신장에서는 충혈과 흑색 대식세포군이 관찰되었다. 정상적인 기능 수행이 어려울 것으로 판단되는 한계농도는 아가미의 경우 0.18mg/$\ell$/21d 간췌장에서는 0.18mg/$\ell$/14d 그리고 신장에서는 0.08mg/$\ell$/14d 였다.

• 한국수산과학회지
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• 제48권1호
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• pp.64-70
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• 2015

We investigated the branchial osmoregulatory response of black sea bream Acanthopagrus schlegelii to short-term (3-48 h) exposure to a hyposaline environment (5 psu). Gill $Na^+/K^+$-ATPase (NKA) activity was decreased after 3 h in fish transferred to 5 psu compared to salt water-acclimated (control) fish, but the level of activity returned to that observed in the control fish at 6 h after transfer. NKA activity increased significantly at 24 h after transfer, but it returned to the level observed in the control fish at 48 h after transfer. Immunohistochemical staining revealed that gill NKA was localized to chloride cells. The number of chloride cells tended to change in parallel with NKA activity. Substantial decreases in plasma $Na^+$, $Cl^-$, and osmolality were observed after 12 h of exposure to 5 psu; however, these parameters began to recover to the values detected in the controls at 24 h after transfer. In conclusion, our results suggest that black sea bream are able to adjust their osmoregulatory mechanisms to shift from hypo- to hyperosmoregulation within 6 h of exposure to a hypoosmotic environment.

• 수산해양기술연구
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• 제55권2호
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• pp.105-120
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• 2019

The species composition and abundance variations of fishery resources in the adjacent marine ranching area, Tongyeong, Korea, were investigated by shrimp trawl, gill net, and longline during the period of July, September, and October in 2016. During the study period, the total catch were 8,522.9 kg with 34 species from the shrimp trawl, 32 species from the gill net, and nine species from the longline. The dominant species were different by gear, which were Hypodytes rubripinnis and Parapercis sexfasciata in the shrimp trawl, Platycephalus indicus and Raja kenojei in the gill net, and Conger myriaster and Scomber Japonicus in the longline. In terms of spatial distribution, Yongchodo showed the highest total catch in number as well as of in weight while Jukdo showed the lowest total catch in number and Bijindo showed the lowest total catch in weight. The amount of demersal fish resources in the survey area estimated as 301 ha, was 99,396 individuals which was converted to be 8,552.9 kg. The amount of demersal fish resources by gear were of trawling area, which area is 127 ha, were 76,251 and 3,489.5 kg, 74 ha in the gill net and longline survey area was 16,213 and 3,457.3 kg, and the other 100 ha area was 6,932 and 1,606.1 kg. In this study, the minimum resources for demersal fish is 61,687 and 4,265.2 kg, and the maximum is 149,439 and 14,197.9 kg.

• 환경생물
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• 제33권1호
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• pp.27-33
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• 2015

2005년 6월, 9월, 12월 및 2006년 3월에 동해안의 감포연안에서 저층삼중자망에 어획된 어류 종 조성을 분석하였다. 조사기간 동안 총 21과 32종, 695마리, 180,012.0 g의 어류가 채집되었다. 채집된 어류 가운데, 가장 많이 채집된 종은 망상어 (Ditrema temminckii)로서 채집 개체수의 52.4%, 생체량의 40.4%를 차지하였다. 그 다음 주요 종으로는 쥐노래미 (Hexagrammos otakii), 조피볼락 (Sebastes schlegelii), 쥐치 (Stephanolepis cirrhifer), 탁자볼락 (Sebastes taczanowskii), 용치놀래기 (Halichoeres poecilopterus) 등이었다. 이들 6종은 전체 개체수의 77.6%, 전체 생체량의 77.5%를 차지하였다. 종수, 개체수 및 생체량은 계절에 따라 변동하였다. 종수는 2005년 6월에 가장 많았고 2006년 3월에 가장 적었다. 개체수와 생체량은 2005년 9월에 가장 많았고, 2005년 6월에 가장 낮았다.

• 대한수의학회지
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• 제56권4호
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• pp.215-221
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• 2016

Due to the shortage of the fingerling/juvenile mud loach, Misgurnus mizolepis in Korea, these fish have been imported from China. However, the mortality rate during and after their transportation is very high. In this study, we examined various physiological and histological parameters to evaluate the effect of salt treatment on the survival and recovery of mud loaches in holding farms during the quarantine process. Glucose, osmolality, $Na^+$, $Cl^-$, and histological changes were assessed for three different salinities. Non-treated fish (control 0.0%) exhibited lower levels of osmolality, and $Na^+$ and $Cl^-$ concentrations compared with those kept in solar salt solution (0.5% and 1.0%). Glucose levels in control fish were higher than those in fish exposed to 0.5% and 1.0% solar salt solution. Histologically, control fish showed thinner epidermis of skin, branchial hyperplasia and lamellar fusion with an abundance of eosinophilic granule cell-like cells. After solar salt solution treatment, damaged gill structures in the fish almost recovered within 5 days. The present study demonstrates that mud loaches transported from China suffer from skin and gill damage and physiological dysfunction which may increase the mortality and morbidity. Moreover, saline treatment might alleviate the stress responses and ionic/osmotic imbalances, and help heal gill damage.

• 한국임상수의학회지
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• 제19권1호
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• pp.14-18
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• 2002

An epidemiological study was performed to evaluate the state of fish diseases between aquaculture fishes in the area of southern Kyeongnam, especially Tongyeong-si, Geoje-si, and Goseong-gun. Examination for a total of 91 cases was carried out for a variety of fishes, including Japanese flounder(Paralichthys olivaceus), rock fish (Sebastes schlegeli), sea bass (Lateolabrax japonicus), sea breams(Pagrus major), and red drum (Sciaenops ocellatus) from November, 1999 to October, 2001. The investigation resulted in exploring 155 infectious diseases, including 62 Vibriosis, 16 Edwasiella infection, 11 Streptococcosis, 7 Pseudotuberculosis, 15 Scuticociliatida infection, 20 Gill flukes, and 9 Trichodiniasis. When the infections were classified according to fish species, Japanese flounder was infected by 30 Vibrio spp., 16 Edwardsiella tarda, 6 Streptococcus spp., 12 Scuticociliatida infection. In case of sea bass, 10 Vibrio spp. was able to isolate out of 10 outbreaks, on the other hand, rock fish was dominantly infected by gill flukes since it was observed 12 out of a total of 20 cases. With reference to seasonal fluctuation, Vibrio spp. infection was continually observed through all seasons but Edwardsiella septicaemia, Streptococcosis and Pseudotuberculosis was mainly isolated in summer and autumn when the water temperature was raised high. Scuticociliatida infection was frequently occurred from late autumn to early spring but gill flukes were watched all the year regardless of temperature. As regards, multiple infection, 47 out of 91 cases was Identified to have involved in more than 2 pathogens, especially Vibrio spp. was highly mixed with other pathogens since 42 out of 47 cases was mixed with Vibrio spp.

• 한국수산과학회지
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• 제9권4호
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• pp.265-271
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• 1976

1. Formalin $50\~60ppm$, malachite green 0.2ppm, dipterex 0.6ppm 되도록 혼합하여 순환수조내의 어류기생성원충류인 Chilodonella, Costia, Trichodine, Ichthyophthirius등을 근절할 수 있었으며 이때 어체의 아가미 조직에 변화는 관찰되지 않았다. 이때에 기생충은 6기간 후부터 구제되었다. $2.130\~140ppm$ formalin에 $24\~48$시간 약욕시킨 잉어의 아가미 상피에 병리학적 변성을 볼 수 있었다. 이 경우 치료약에 장시간 약욕시킴으로서 새엽상피가 변성 괴사되고 새엽지주세포가 떨어졌다.

• 한국어병학회지
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• 제23권3호
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• pp.429-440
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• 2010

We constructed a rock bream (Oplegnathus fasciatus) gill cDNA library and a total of 1450 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 1450 EST clones, 1022 EST clones showed significant homology to previously described genes while 428 ESTs were unidentified, and 259 clones were hypothetical, or unnamed proteins. Encoding 313 different sequences were identified as putative bio-defense genes or genes associated with immune response.