• 제목/요약/키워드: Fish consumption

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Development of Species-Specific PCR Primers for the Rapid and Simultaneous Identification of the Six Species of Genus Takifugu

  • Dong, Chun Mae;Park, Yeon Jung;Noh, Jae Koo;Noh, Eun Soo;An, Cheul Min;Kang, Jung-Ha;Park, Jung Youn;Kim, Eun-Mi
    • 한국발생생물학회지:발생과생식
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    • 제23권4호
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    • pp.367-375
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    • 2019
  • Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

운동 여부에 따른 성인 남자의 식습관, 식행동 및 건강관심도에 관한 연구 (A Study on Dietary Behaviors, and the Health of Male Adults according to Their Exercising Habits)

  • 정근희;신경옥;최경순;유광욱;유재현
    • 한국식품영양학회지
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    • 제26권3호
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    • pp.329-338
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    • 2013
  • This study is conducted to compare the problems affecting dietary habits and health status between a long-term exercise group and non-exercise group of males older than 50 years. Most subjects of the two groups consider regular exercise to be the most important factor for maintaining health, and recognize hypertension as the number one concern. The most common nutritional supplement among subjects is multiple vitamins, and sleeping time range from 6~8 hours. Breakfast fasting rate was 15.9% among non-exercise group (NEG), where overeating and eating out rates were higher among exercise group (EG) when comparing to NEG. Fasting rates of breakfast, and consumption rates of milk and dairy products, vegetables such as kimchi, and fruits and fruit juices are higher among the EG. Overeating and eating out rates are lower among NEG, whereas eating rates of proteins like meat, fish, eggs, beans, fried foods, high-fat meats carbonated drinks, ice cream, and salt intakes are higher. Rates of health, dietary habits, and nutrient intakes are higher among EG. Energy intakes among the two groups were below the standard level, Vitamin $B_2$ intake is low in EG, and folic acid and calcium intakes were higher in NEG. The exercising group care more about health, eating habits and nutrient intakes, and low smoking rates are being observed. Thus, specific dietary improvement programs for adult men, are required, and it is necessary to consider the practice of nutrition education.

경기도지역 어린이집과 유치원 급식의 소금함량 및 유아 식사준비자의 나트륨 섭취관련 식태도 조사 (A Survey on the Salt Content of Kindergarten Lunch Meals and Meal Providers' Dietary Attitude to Sodium Intake in Gyeonggi-do Area)

  • 김진남;박서연;안소현;김혜경
    • 대한지역사회영양학회지
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    • 제18권5호
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    • pp.478-490
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    • 2013
  • Dietary habit of excess sodium consumption is formed mainly by excessive salt intake from the younger age and this may lead to hypertension, stroke, and stomach cancer. This study was performed to estimate the salt content in kindergarten meals and provide basic data on meal providers' dietary attitude to sodium intake for nutrition education. We collected data on161 food items from 16 institutions in Gyeonggi-do and salt content was calculated from salinity and weight of individual food items. The average salt content from lunch meals was 2.2 g, which was about daily adequate intake of sodium for children aged 3 to 5 years old. Greatest contributor to the salt content in a meal was soup and stew (47.8%). The most salty dishes were sauces and kimchi followed by stir-fried food, deep-fried food, braised food, and grilled food. The salt content was higher in soup and stew despite of low salinity, due to the large quantity per serving. The salt contents of soups and kimchi were 40.6% and 14.3%, respectively of the total salt content in dish groups. Staff members and caregivers at home who prepared food for the child showed preference for one-dish rice meal, dried fish and salted mackerel, and broth when eating soup, stew, and noodles. Caregivers showed higher sodium index score and had higher preference for processed food such as Ramen, canned food, and ham compared with staff members (p < 0.05). These results suggested that monitoring salt content of kindergarten meals and nutrition education for those prepare meals for children are needed to lower sodium intake in childhood.

인천지역 일부 고등학생의 아침결식에 따른 식행동 및 식사의 질 평가 (Evaluation of Dietary Behaviors and Dietary Quality of High School Students in Incheon according to Breakfast Skipping)

  • 이현정;김은미;김미현
    • 한국식생활문화학회지
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    • 제34권6호
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    • pp.726-738
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    • 2019
  • This study examined the dietary behaviors and dietary quality of high school students according to the number of times they skipped breakfast. A total of 474 high school students (225 boys and 249 girls) residing in Incheon participated in this survey. The subjects were divided into the three groups according to the frequency of eating breakfast; Regualr (eating breakfast everyday, n=226), Irregualar (eating breakfast 1~6 times/week, n=143, and Skipping (skipping breakfast everyday, n=105). The dietary quality was assessed using a nutrient quotient for adolescents (NQ-A). A higher monthly allowance and a higher rate of working mothers were significantly associated with a higher frequency of skipping breakfast. A higher intake frequency of processed beverages, and street food was significantly associated with a higher frequency of skipping breakfast. Students who had a higher rate of breakfast skipping had significantly lower intake frequency of fruits, white milk, bean and bean products, and fish. The high frequency of skipping breakfast among high school students was associated with lower dietary quality, as illustrated by the low total NQ-A score and sub group score including balance, environment, and practice. This information can inform efforts to provide nutrition education program to promote breakfast consumption and guidelines to students who skipped breakfast frequently.

LED와 메탈핼라이드 집어등을 겸용한 갈치채낚기 어선의 조업 성능 (Operating Performance of hair-tail angling vessel using the LED and metal halide fishing lamp combination)

  • 안희춘;배봉성;이경훈;박성욱;배재현
    • 수산해양기술연구
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    • 제48권4호
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    • pp.337-345
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    • 2012
  • One of representative Korean fisheries, jigging and angling has 5,700 vessels for squid and hairtail. Hairtail angling is the most typical fishery in Jeju and has an enormous impact on regional economy. However, the price hike in oil and labor costs triggered the necessity of developing a high efficient and energy saving fish luring lamp in recent times. For that reason, this study aimed to analyze the fishing performance of the aircooled LED lamp targeting hairtail angling fishery. The experiment was conducted from September through October in 2009-2011 for a 9.77 ton of fishing vessel setting up 100 lamps. The fishing performance was tested compared with 6 vessels using metal halide lamps in the same waters. As the LED lamp's performance goes up, different lamps were used in 2008 for 80W, 2009 for 120W and 2011 for 180W respectively. The catch and CPUE of the experimental vessel have gradually increased respectively taking the $4^{th}$ and $6^{th}$ place in 2009, the $4^{th}$ and $2^{nd}$ place in 2010, the $1^{st}$ and $1^{st}$ place in 2011 among the 7 vessels. In summary, the LED fishing lamp showed higher fishing performance than comparison groups. It saved 33% of oil consumption and cut down on operating expenses and greenhouse gases emission.

메탈핼라이드 집어등용 전자식 안정기 개발 (Development of a metal-halide lamp's electronic ballast)

  • 박성욱;배봉성
    • 수산해양기술연구
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    • 제49권2호
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    • pp.116-125
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    • 2013
  • Jigging and angling fishery is prevalent in the East Sea of Korea and this fishery needs many lamps to attract the fish. And the fishing boat uses 24~47 ballasts by the vessel's tonnage to turn on the fishing lamp. A 3.5kW magnetic-type ballast being currently used at many fishing boats can drive two 1.5kW metal-halide lamps. Meanwhile, this ballast has large weight (25kg) and volume. Therefore it is one of reason for the over-consumption of energy and the fire, resulted from overheat and electrical short, occurs occasionally because the ballast is installed at narrow and hot engine room. In addition, most of magnetic ballast has several problems such as periodic condenser replacement, low energy efficiency and making lamp short life, etc. So it is necessary to improve or develop newly the electronic ballast, which has to be smaller, lighter and more efficient. An electronic ballast was designed for the fishing boat by considering duration and electromagnetic interference in the study. Its weight and volume are respectably 40% and 66% compared to current ballast on the basis of PCB. The metal-halide lamp's spectrum of the designed ballast was nearly same to that of the current ballast in the test of lighting. In particular, the light stability was improved and there isn't any radio interference. As mentioned above, it is expected that the developed electronic ballast can replace current magnetic ballast because of many advantages related to energy-saving.

24시간 소변 분석, 식사기록법 및 음식섭취빈도조사에 의한 여자 성인의 나트륨 섭취량 조사 (Estimation of Sodium Intake of Adult Female by 24-Hour Urine Analysis, Dietary Records and Dish Frequency Questionnaire (DFQ 55))

  • 신은경;이혜진;이중정;안문영;손숙미;이연경
    • Journal of Nutrition and Health
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    • 제43권1호
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    • pp.79-85
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    • 2010
  • This study assesses the sodium intake of adults by 24-hour urine analysis, dietary records review and a food intake questionnaire. Subjects were 236 adult female in Daegu. The results are summarized as follows: Sodium intake as indicated by the 24-hour urine analysis was $5,805.4\;{\pm}\;3836.8\;mg$. This was significantly higher than intake indicated by dietary records ($4415.4\;{\pm}\;1935.1\;mg$) and the dish frequency questionnaire (DFQ 55) ($4293.5\;{\pm}\;1526.5\;mg$). The results of the 24-hour urine analysis and DFQ 55 showed that sodium intake was higher for the 65-to-74-year-old age group than for other age groups (p < 0.05, p < 0.05). Review of dietary records to examine typical sodium intake by food groups showed that 53.7% of the sodium consumed by subjects in all age groups came from seasonings and spices ($2399.0\;{\pm}\;1526.5\;mg$). The analysis of sodium intake by food groups using DFQ 55 showed 34.2% of their sodium came from consumption of kimchi (p < 0.001) and kimch, soup, stew and fish jorim accounted for 57.8% of total sodium intake. The results indicate positive correlation between age and sodium intake, as shown by the 24-hour urine analysis and food intake questionnaire (p < 0.05). Therefore, these results would be valuable as basic data for planning nutrition education for sodium intake reduction.

광양만 잘피밭에 서식하는 붕장어 (Conger myriaster)의 식성 (Feeding Habits of Conger myriaster in the Eelgrass (Zostera marina) Bed in Kwangyang Bay)

  • 허성회;곽석남
    • 한국수산과학회지
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    • 제31권5호
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    • pp.665-672
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    • 1998
  • 1994년 1월부터 1994년 12월까지 광양만 대도주변 잘 피밭에서 채집된 붕장어의 식성을 조사하였다. 붕장어의 주요 먹이는 어류, 새우류 및 게류였다. 그밖에 옆새우류, 복족류, 두족류, 갯지렁이류, 곤쟁이류, 쿠마류, 등각류 등이 위내용물 중 발견되었다. 붕장어가 성장함에 따라 먹이생물의 조성이 점차 변하였다. 체장이 작은 붕장어는 새우류, 어류, 게류 그리고 옆새우류를 고르게 먹었으나, 체장이 증가하면서 옆새우류와 새우류의 점유율은 점차 감소하였고, 어류의 점유율은 점차 증가하였다. 붕장어는 계절에 따른 환경 생물의 조성과는 관계없이 특정 생물을 먹이로 선호하는 경향을 보였다. 특히 어류는 다른 환경 생물에 비해 잘피밭 환경에서 출현량이 많지 않았음에도 불구하고 연중 지속적으로 선택되는 먹이생물이었다.

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노인 중풍 발생에 미치는 요인 분석 (Analisis of chief influencing factor on aged man's cerebrovascular accidents - case control study -)

  • 최영미;유태식;이종섭
    • 농촌의학ㆍ지역보건
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    • 제23권1호
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    • pp.51-64
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    • 1998
  • This study was carried out during 3 years from March 1992 to March 1995, 140 healthy people and 140 C.V.A patients were investigated, at an oriental clinic in Taejon. The data collected was analysed using a SAS. The results are as follows. 1. The Relationships between patients who have undergone one or more abortion procedures and C.V.A occurrence. A statistical significant difference was recorded between the control group and the outpatients group of P<0.05 2. Number of years after initial C.V.A diagnosis before C.V.A symptom onset. within 1 years 52% (2-3 years, 3-4 years, 4-5 years, 5 years and over) 3. Additional diseases occurring after initial stroke 1 disease 50.7% (2, 3, 4, 5 or more) 4. Alcohol Consumption "A statistical significant difference was recorded between the control group and the outpatients group of" P<0.01 5. Previous Treatment Experience for stoke outpatients Oriental Hospital, clinic 64.3% (General Hospital, clinic, a public health center drug store, home remedies, etc) 6. Exercise Frequency Outpatients Regular 28.6%, Irregular 71.4% Control sample Regular 27%, Irregular 72.9% 7. Degree of Appetite "A statistical significant difference was recorded between the control group and the outpatients group of" P<0.01 8. People who include fish in their diet "A statistical difference was recorded between the control group and the outpatients group of" P<0.01.

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Association between Amalgam Tooth Fillings and Blood Mercury Levels in Children

  • SaKong, Joon;Choi, Youn-Hee;Chung, Sun-Young;Kwon, Ho-Jang;Karmaus, Wilfried;Merchant, Anwar T.;Ha, Mi-Na;Hong, Yun-Chul;Kang, Dong-Mug;Song, Keun-Bae
    • International Journal of Oral Biology
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    • 제36권3호
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    • pp.123-128
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    • 2011
  • The notion that dental amalgam is a potential source of mercury exposure remains a controversial issue. However, there are few epidemiological analyses that have addressed whether this occurs in children. We aimed in our current study to identify the relationship between dental amalgam filling surfaces and the blood mercury levels in a cohort of 711 South Korean children aged between 8-9 years. Oral examinations were conducted to detect the number of amalgam filling surfaces on the teeth of these individuals. Blood samples were also taken from these children to assess the levels of mercury accumulation in the body. The amalgam filling surfaces were classified into four groups based on their number: 0 (n = 368), 1-5 (n = 219), 6-10 (n = 89), and 11+ (n = 35). The blood mercury levels in the children with more than 10 amalgam surfaces was 0.47 ${\mu}g$/L higher on average than those with no amalgam surfaces after adjusting for the frequency of fish or seafood consumption, age, and gender (P < 0.05). We found from our data that a higher number of dental amalgam fillings correlated with a higher blood mercury level in Korean children. Further studies are needed to investigate whether these elevated mercury levels exert neurotoxic or nephrotoxic effects.