• Korean Journal of Microbiology
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• v.39 no.1
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• pp.27-35
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• 2003

No currently available methods to monitor pathogenic protozoa, Cryptosporidium and Giardia in environmental water come close to acceptable sensitivity, specificity and reproducibility, and so it has to be accompanied by thorough quality control and performance evaluation to credibly predict the distribution of them. We collected surface water samples from the Han River and spiked our prepared (oo)cysts, determined Matrix Spike recoveries using USEPA Method 1623 and considered what factors influence MS recovery and validity. As a result, average 46% of spiked oocysts and 60% of spike cysts were recovered, but repetitive sampling and statistical approach seemed to be necessary to determine the environmental pollution level of two protozoa as their variation coefficients was so much as 35oio and 26%. And MS recoveries with two acid dissociations during immunomagnetic separation were improved more 10% than that with one dissociations and the use of spiked suspension enumerated by flow cytometry instead of manual preparation enhanced the validity and reliability in spiking tests. Because fluorescence characteristics of (oo)cysts stained on well slides with FITC-labeled monoclonal antibodies and DAPI was not always same, well Elides from spiked field samples were helpful to evaluate the performance of staining. We found many (oo)cyst-like objects with typical fluorescence, not (co)cysts, from the Han River water samples, and then it was concluded that nuclei staining by DAPI (4',6-diamidino-2-phenylindole) and examination by Differential Interference Contrast Microscope should be critical for valid identification.

• Progress in Superconductivity
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• v.9 no.1
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• pp.68-73
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• 2007

We fabricated the in-situ $MgB_2$ wires using the powder-in-tube method and investigated the effects of sintering temperature and SiC contents on the microstructure and superconducting properties. Pure $MgB_2$ wires and 5, 10, 20 wt.% SiC doped $MgB_2$ wires were sintered at $600-1000^{\circ}C$ for 30 minutes in Ar atmosphere. We found that $MgB_2$ phase was mostly formed at the sintering temperature of $700^{\circ}C$ and above, and the critical temperature ($T_c$) increased with increasing sintering temperature. For the $MgB_2$ sintered at $850^{\circ}C$, the highest critical current density ($J_c$) was obtained to be $3.7{\times}10^5\;A/cm^2$ at 5 K and 1.6 T by a magnetic properties measurement system (MPMS). The addition of SiC to the $MgB_2$ wires changed microstructure and critical properties. SEM observation showed that the $MgB_2$ core had considerable micro-cracks in undoped wire and the density of micro-cracks decreased with increasing SiC contents. The critical temperature decreased as the SiC contents increased, on the other hand, the critical current density of SiC doped $MgB_2$ wires in high magnetic field was enhanced compared to that of undoped $MgB_2$ wires.

• Journal of the Korean Society of Groundwater Environment
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• v.7 no.2
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• pp.89-96
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• 2000

Slug tests are the most widely used field method for quantification of hydraulic conductivity of porous media. Well recovery is affected by well casing, borehole radii, screened length, hydraulic conductivity, and specific storage of porous media. In this study, a new slug tests model was developed through finite element approximation and the validity and usefulness of the model were tested in various ways. Water level fluctuation in a well under slug test and cons-equent groundwater flow in the surrounding porous medium were appropriately coupled through estimation of well-flux using an iteration technique. Numerical accuracy of the model was verified using the Cooper et al. (1967) solution. The model has advantages in simulations for monitored slug tests, partial penetration, and inclusion of storage factor. Volume coverage of slug tests is significantly affected by storage factor. Magnitude and speed of propagation of head changes from a well increases as storage factor becomes low. It will be beneficial to use type curves of monitored head transients in the surrounding porous formation for estimation of specific storage. As the vertical component of groundwater flow is enhanced, the influence of storage factor on well recovery decreases. For a radial-vertical flow around a partially penetrated well, deviations between hydraulic estimates by various methods and data selection of recovery curve are negligible on practical purposes, whereas the deviations are somewhat significant for a radial flow.

• Research in Plant Disease
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• v.18 no.3
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• pp.210-216
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• 2012

Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313, which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical 'fluazinam' was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50% diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313, seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.260-260
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• 2013

Multiferroic materials have attracted much attention due to their fascinating fundamental physical properties and technological applications in magnetic/ferroelectric data-storage systems, quantum electromagnets, spintronics, and sensor devices. Among single-phase multiferroic materials, $BiFeO_3 $ is a typical multiferroic material with a room temperature magnetoelectric coupling in view of high magnetic-and ferroelectric-ordering temperatures (Neel temperature $T_N$~647 K and Curie temperature $T_C$~1,103 K). Rare-earth ion substitution at the Bi sties is very interesting, which induces suppressed volatility of Bi ion and improved ferroelectric properties. At the same time, Fe-site substitution with magnetic ions is also attracting, and the enhanced ferromagnetism was reported. In this study, $Bi_{1-x}Dy_xFe_{0.95}Co_{0.05}O_3$ (x=0, 0.05 and 0.1) bulk ceramic compounds were prepared by solid-state reaction and rapid sintering. High-purity $Bi_2O_3$, $Dy_2O_3$, $Fe_2O_3$ and $Co_3O_4$ powders with the stoichiometric proportions were mixed, and calcined at $500^{\circ}C$ or 24 h to produce $Bi_{1-x}Dy_xFe_{0.95}Co_{0.05}O_3$. The samples were immediately put into an oven, which was heated up to $800^{\circ}C$ nd sintered in air for 30 min. The crystalline structure of samples was investigated at room temperature by using a Rigaku Miniflex powder diffractometer. The field-dependent magnetization measurements were performed with a vibrating-sample magnetometer. The electric polarization was measured at room temperature by using a standard ferroelectric tester (RT66B, Radiant Technologies).

• Development and Reproduction
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• v.8 no.1
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• pp.1-9
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• 2004

The potential importance of proteomic approaches has been clearly demonstrated in other fields of human medical research, including liver and heart disease and certain forms of cancer. However, reproductive researches have been applied to proteomics poorly. Proteomics can be defined as the systematic analysis of proteins for their identity, quantity, and function. It could increase the predictability of early drug development and identify non-invasive biomarkers of toxicity or efficacy. Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis(2DE) and MALDI-TOF(matrix-assisted laser desorption ionization-time of flight) MS(mass spectrometry) or protein chip array and SELDI-TOF(surface-enhanced laser desorption ionization-time of flight) MS. In addition understanding the possessing knowledge of the developing biomarkers used to assess reproductive biology will also be essential components relevant to the topic of reproduction. The continued integration of proteomic and genomic data will have a fundamental impact on our understanding of the normal functioning of cells and organisms and will give insights into complex cellular processes and disease and provides new opportunities for the development of diagnostics and therapeutics. The challenge to researchers in the field of reproduction is to harness this new technology as well as others that are available to a greater extent than at present as they have considerable potential to greatly improve our understanding of the molecular aspects of reproduction both in health and disease.

• The Journal of Engineering Geology
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• v.20 no.3
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• pp.243-255
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• 2010

The slope design of an open-pit mine must consider economical efficiency and stability. Thus, the overall slope angle is the principal factor because of limited support or reinforcement options available in such a setting. In this study, slope displacement, as monitored by a GPS system, was analyzed for a coal mine at Pasir, Indonesia. Predictions of failure time by inverse velocity analysis showed good agreement with field observations. Therefore, the failure time of an unstable slope can be roughly estimated prior to failure. A GIS model that combines fuzzy theory and the analytical hierarchy process (AHP) was developed to assess slope instability in open-pit coal mines. This model simultaneously considers seven factors that influence the instability of open-pit slopes (i.e., overall slope gradient, slope height, surface flows, excavation plan, tension cracks, faults, and water body). Application of the proposed method to an open-pit coal mine revealed an enhanced prediction accuracy of failure time and failure site compared with existing methods.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.237-244
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• 2008

This study was conducted to investigate the effects of the cropping system of forage crops and application of cattle slurry on productivity of forage crops and soil fertility. The field experiments were conducted on the silt clay loam at Gongiam, Kwangju, Kyung-gi province in Korea for two years. This study was arranged in split plot design with three replicates. Main plots were the cropping systems, such as single crop and mixed crops. Subplots were the application rate of cattle slurry, such as 0, 150 and 300 kg N/ha. The yields of dry matter (DM) and nitrogen (N) were hardly influenced by the cropping system, whereas DM and N yields enhanced as increasing the rates of cattle slurry application (p<0.05). The contents of crude protein (CP) and total digestible nutrients (TDN) were hardly influenced by the cropping system, whereas CP content increased as increasing the rates of cattle slurry application (p<0.05). TDN was not differentially influenced by cattle slurry application. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were hardly influenced by the cropping system and application of cattle slurry. Organic matter (OM) content in soil samples collected at the end of the experiment were remarkably higher than those in the beginning of the experiment. The OM content of soil was significantly increased by application of cattle slurry (p<0.05).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.14-18
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• 2018

Generally. the Ohmic's law is an important factor to increase the conductivity in a micro device. So it is also known that the Ohmic contact in a semiconductor device is import. The PN junction as a structure of semiconductor involves the depletion layer, and this depletion layer induces the non linear electrical properties and also makes the Schottky contact as an intrinsic characteristics of semiconductor. To research the conduction effect of insulators in the semiconductor device, $SiO_2$ thin film and $V_2O_5/SiO_2$ thin film were researched by using the current-voltage system. In the nano electro-magnetic system, the $SiO_2$ thin film as a insulator had the non linear Schottky contact, and the as deposited $V_2O_5$ thin film had the linear Ohmic contact owing to the $SiO_2$ thin film with superior insulator's properties, which decreases the leakage current. In the positive voltage, the capacitance of $SiO_2$ thin film was very low, but that of $V_2O_5$ thin film increased with increasing the voltage. In the normal electric field system, it was confirmed that the conductivity of $V_2O_5$ thin film was increased by the effect of $SiO_2$ thin film. It was confirmed that the Schottky contact of semiconductors enhanced the performance of electrical properties to increased the conductivity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.146-159
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• 2018

The purpose of this study was to investigate the effects of Mathematical Activities using 4D-frame on 5-year-old children's mathematical ability and attitude towards Mathematics. For the study, E, K, and Y preschools located in S city were selected: 28 children from Y preschool as the experimental group(I: n=14, II: n=14) and 28 children from E and K preschool as the comparative group(III: n=14, IV: n=14). During the 8 weeks, the experimental group performed Mathematical Activities using 4D-frame and in the comparison group, Nuri-Curriculum's mathematical activities and mathematics-science integration programs were conducted. The analysis of covariance(ANCOVA) was conducted with the pre-test scores of the experimental group(I, II) and the comparison group(III, IV) as covariance. The results showed that there were statistically significant differences between the experimental and comparative groups. Mathematical Activities for Young Children using 4D-frame enhanced 5-year-old children's mathematical ability and attitude towards Mathematics. The results of this study provide an understanding of the efficiency of early childhood mathematical activities using the 4D-frame in early childhood education and provide basic data for the improvement of mathematical ability and attitude of young children and the applicable educational methods at the field.