Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Biocontrol Efficacy of Endophytic Bacteria Flavobacterium hercynim EPB-C313 for Control of Chinese Cabbage Clubroot

Flavobacterium hercynium EPB-C313 균주를 이용한 배추 뿌리혹병 생물적 방제

  • Hahm, Soo-Sang (Chungnam Agricultural Research and Extension Services) ;
  • Kim, Jong-Tae (Chungnam Agricultural Research and Extension Services) ;
  • Han, Kwang-Seop (Chungnam Agricultural Research and Extension Services) ;
  • Kim, Byung-Ryun (Chungnam Agricultural Research and Extension Services) ;
  • Kim, Hong-Kyu (Chungnam Agricultural Research and Extension Services) ;
  • Nam, Yun-Kyu (Chungnam Agricultural Research and Extension Services) ;
  • Yu, Seung-Hun (Department of Applied Biology, Chungnam National University)
  • Received : 2012.06.19
  • Accepted : 2012.08.21
  • Published : 2012.09.30
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Abstract

Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313, which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical 'fluazinam' was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50% diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313, seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage.

Plasmodiophora brassicae에 의한 배추 뿌리혹병은 배추뿐만 아니라 순무, 양배추에 발병하여 큰 피해를 주는 병원균이다. 배추 뿌리혹병균에 길항효과가 있는 미생물을 선발하기 위하여 배추 조직에서 분리한 Flavobacterium hercynium EPB-C313 균주의 항균활성 검정을 하였고, 포장시험을 통하여 배추 뿌리혹병에 대한 방제효과를 조사하였다. F. hercynium EPB-C313의 균체, 배양액, 배양여액를 배추 뿌리혹병 휴면포자와 혼합 처리하여 24시간 후 조사하였을 때 각각 90.4%, 36.8% 및 26.0%의 휴면포자가 불활성화 되었다. 온실 포트검정에서는 배추의 어린묘를 F. hercynium EPB-C313 배양액에 침지한 후 정식하면 뿌리혹 형성 억제율이 100%로 대조약제 fluazinam액상수 화제의 91.7%보다 우수한 효과를 나타내었다. 배추 뿌리혹병이 50% 이상 발병되었던 재배포장에 F. hercynium EPB-C313 혼합펠렛을 토양혼화 하고, F. hercynium EPBC313 배양액의 유묘관주 및 정식 10일 후 1회 토양관주하면 63.7%의 방제효과를 보였다. 이상의 결과로 볼 때 F. hercynium EPB-C313 균주는 매우 유용한 배추 뿌리혹병 생물적 방제제로 판단된다.

Keywords

References

  1. Arie, T., Kobayashi, Y., Okada, G., Kono, Y. and Yamaguchi, I. 1988. Control of soilborne clubroot disease of cruciferous plant by epoxydon from Phoma glomerata. Plant Pathol. 47: 743-748.
  2. Barraquio, W. L., Revilla, L. and Ladha, J. K. 1997. Isolation of endophytic diazotrophic bacteria from well and rice. Plant Soil. 194: 15-24. https://doi.org/10.1023/A:1004246904803
  3. Chang, S. W., Hong, S. S., Kim, S. K., Kim, H. D. and Yi, E. S. 2000. Improvement of control effect by change of chemical application method on clubroot disease of Chinese cabbage caused by Plasmodiophora brassicae in field. Res. Plant Dis. 6: 39-42. (In Korean)
  4. Dymock, D., Weightman, A. J., Scully, C. and Wade, W. G. 1996. Molecular analysis of micoflora associated with dentoalveolara abscesses. J. Clin. Microbiol. 34: 537-542.
  5. IFOAM. 2010. The World of Organic Agriculture : Statistics and Emerging Trends. (In Korean)
  6. James, E. K. and Olivares, F. L. 1998. Infection and colonization of sugar cane and other graminaceous plants by endophytic diazotrophs. Criti. Rev. Plant Sci. 17: 77-119. https://doi.org/10.1016/S0735-2689(98)00357-8
  7. Kim, C. H., Cho, W. D. and Kim, H. M. 2000a. Yield loss of spring Chinese cabbage as affected by infection time of clubroot disease in fields. Res. Plant Dis. Res. 6: 23−26. (In Korean)
  8. Kim, C. H., Cho, W. D. and Kim, H. M. 2000b. Some environmental factors affecting germination and survival of resting spores of Plasmodiophora brassicae. Korean J. Pesti. Sci. 4: 66-71. (In Korean)
  9. Kim, C. H., Cho, W. D. and Kim, H. M. 2000c. Distribution of Plasmodiophora brassicae.causing clubroot disease of Chinese cabbage in soil. Res. Plant Dis. 6: 27-32. (In Korean)
  10. Kim, C. H., Cho, W. D. and Lee, S. B. 2003. Review of researches on clubroot disease of Chinese cabbage in Korea and future tasks for its management. Res. Plant Dis. 9: 57-63. (In Korean) https://doi.org/10.5423/RPD.2003.9.2.057
  11. Kim, C. H., Cho, W. D. and Yang, J. M. 1999. Effects of plant age, inoculum concentration, and inoculation method on root gall development of clubroot disease of Chinese cabbage caused by Plasmodiophora brassicae. Plant Dis. Agric. 5: 90-94. (In Korean)
  12. Narisawa, K., Ohki, K. T. and Hashiba, T. 2000. Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 49: 141-146. https://doi.org/10.1046/j.1365-3059.2000.00425.x
  13. Narisawa, K., Tokumasu, S. and Hashiba, T. 1998. Suppression of clubroot formation in Chinese cabbage by the root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 47: 206-210. https://doi.org/10.1046/j.1365-3059.1998.00225.x
  14. Oh, J. H., Cho, J. H., Kim, B. G., Chae, J. C., Chung, G. U., Hwang, C. H. and Kim, D. W. 1997. Environmental factors favoring the disease development and chemical control of clubroot (Plasmodiophora brassicae) in Chinese cabbage. Korean J. Plant Pathol. 13: 244−247. (In Korean)
  15. Takahashi, K. and Yamaguchi, T. 1987. An improved method for estimating the number of resting spores of Plasmodiophora brassicae in soil. Ann. Phytopathol. Soc. Japan 53: 507-515. https://doi.org/10.3186/jjphytopath.53.507
  16. Tanaka, S. 1996. Recent progress in studies on clubroot disease of crucifers. Shokubutsu Bokei (Plant Protection) 50: 281-284.
  17. Zhang, X. Z., Lee, S. U., Kim, J. S., Yoon, Choi, G. S., Kim, H. K. and Kim, B. S. 2005. Control efficacy of flusulfamide GR on Chinese cabbage clubroot caused by Plasmodiophora brassicae. Res. Plant Dis. 11: 43-47. https://doi.org/10.5423/RPD.2005.11.1.043

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