Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of Pathogens Causing Grape Rot on 'Red Glove' Variety Imported from Chile

칠레산 수입포도 '레드글로브' 품종에 발생한 부패병 병원균의 동정

  • Song, Min-Ji (Department of Plant Medicine, Chungbuk National University) ;
  • Lee, Hyok-In (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Yea, Mi-Chi (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Dae-Ho (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
  • 송민지 (충북대학교 식물의학과) ;
  • 이혁인 (농림수산검역검사본부 식물검역부) ;
  • 예미지 (농림수산검역검사본부 식물검역부) ;
  • 김대호 (농촌진흥청 미생물은행) ;
  • 홍승범 (농촌진흥청 미생물은행) ;
  • 차재순 (충북대학교 식물의학과)
  • Received : 2012.07.09
  • Accepted : 2012.09.26
  • Published : 2012.09.30
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Abstract

Post-harvest rot of grape causes a severe economic loss and lower of the grape quality. It is also one of the important limiting factors for grape export. Grape rots and their casual agents on 'Red Globe' variety imported from Chile were identified. Grapes shown rotting symptom were collected from the storages near the import harbor. The 3 different rots were identified on the imported 'Red Globe'; melting decay, gray mold, and blue mold. A bacterium that isolated from a typical melting decay symptom was identified as Gluconobacter cerinus on basis of its nucleotide sequence of 16S rDNA and fatty acid profile. By inoculation on grape, it caused cracking and dissolution of epidermis of grape which were the characteristics of melting decay. Botrytis cinerea and Penicillium expansum were isolated from grapes showing gray mold and blue mold. The 2 fungal isolates were identified on basis of their morphological characteristics and nucleotide sequence of their beta-tubulin genes. They showed strong pathogenicity on 'Campbell Early' variety that is a major table grape in Korea.

수확 후 부패는 큰 경제적 손실과 포도의 품질을 낮추는 요인이다. 또한 이는 포도 수출의 중요한 제한 요인 중 하나이다. 본 연구에서는 칠레로부터 수입한 '레드글러브' 품종의 부패병과 원인 병원균을 동정하였다. 포도 수입항 근처의 저장창고로부터 부패병이 발생한 포도를 수집하였다. 수입포도 '레드글러브' 품종에서 melting decay, 잿빛곰팡이병, 푸른곰팡이병의 3가지 부패병이 관찰되었다. 전형적인 melting deay로부터 분리한 세균은 16S rDNA의 염기서열과 지방산 프로필을 기초로 Gluconobacter cerinus로 동정하였다. 건전한 포도에 접종에 의해 분리균은 melting decay의 특징인 포도 알 표피의 열과와 괴저를 유도하였다. 잿빛곰팡이병과 푸른곰팡이병 병징을 보이는 포도로부터 Botrytis cinerea와 Penicillium expansum을 분리하였다. 이 2가지 균류는 형태적 특징과 beta-tubulin 유전자의 염기서열을 기초로 동정하였다. 이들은 한국에서 가장 많이 생산되는 '캠벨얼리' 품종에서 강한 병원성을 보여 주었다.

Keywords

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  1. Post-harvest Decay of 'Campbell Early' Grape vol.20, pp.4, 2014, https://doi.org/10.5423/RPD.2014.20.4.275
  2. Molecular Phylogenetic Analysis of Botrytis cinerea Occurring in Korea vol.42, pp.2, 2014, https://doi.org/10.4489/KJM.2014.42.2.138