• Title/Summary/Keyword: Field Trial

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Pregnancy Rate of In Vitro Produced Korean Cattle Embryos according to Transport Time Course

  • Park, Hyo-Young;Kim, Eun-Young;Kim, Young-Hun;Mun, Seong-Ho;Oh, Chang-Eon;Han, Young-Joon;Kim, Nam-Hyung;Lee, Sung-Soo;Ko, Moon-Suck;Riu, Key Zung;Park, Se-Pill
    • Reproductive and Developmental Biology
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    • v.33 no.4
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    • pp.257-262
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    • 2009
  • This study was to investigate pregnancy rate of IVM/IVF/IVC Korean cattle (registered in government) embryos according to transport time course. For the production of embryos, oocytes recovered from slaughtered excellent grade cow and highly motile frozen-thawed bull semen (purchased from LIMC, KPN#497) was used. In vitro produced embryos were cultured in CR1aa medium for 8 days and some of them were frozen. The rate of average cleavage (>2-cell) was 83.0% (308/371) and blastocyst rate at day 8 was 34.7% (107/308). Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. These embryos were produced in a laboratory, embryo transfer (ET) was planned in 10 areas of the remote island (Jeju) from the laboratory by airplane. Thus, we examined the pregnancy rate in recipient cow according to embryo of transport time course before ET. From embryo transferred 44 recipient cows, overall pregnancy was 40.9% (18/44), these 18 cows were all calved [single, 94% (17/18); twin, 6% (1/18)] and total embryo implantation rate was 26% (19/66). Comparing transport time in the base of 6 hr, pregnancy rate in ET group required less 4 hr (60%, 9/15) was significantly higher than that required more 6 hr (26.3%, 5/19). In direct ET of freezing embryos, the pregnancy rate was 40% (4/10). However, it was difficult to find the meaning of temperature, pH and corpus luteum quality of recipients on comparison of pregnancy rate. When the cell death level of embryos according to storage time in thermos (straw container) before ET was measured by TUNEL staining, apoptotic index was increased with storage time-dependent. These results demonstrated that long distance transfer of IVM/IVF/IVC embryos is possible and the time of embryo transport is very important for the pregnancy rate on field trial.

Agronomic Characterization of Sorghum bicolor (L) Moench Germplasm Collected in Gangwon-do for Bio-energy Crop (바이오에너지 자원으로서 강원도 지역에서 수집된 수수의 작물학적 특성 평가)

  • Seong, Eun-Soo;Yoo, Ji-Hye;Lee, Jae-Geun;Hwang, In-Seong;Kim, Nam-Jun;Kim, Myong-Jo;Lee, Ju-Kyung;Ghimire, Bimal Kumar;Lim, Jung-Dae;Yu, Chang-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.151-158
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    • 2011
  • Forty nine accessions of the Sorghum bicolor (L) Moench collected from different agronomical zone of Gangwondo province, Korea were characterized morphologically on field trial. Plant length, stem diameter, tiller number, ear type, ear length, ear width, leaves length, leaves width and leaf color differed significantly in the cultivated accessions. Out of forty nine test accessions, six accessions (12.2%) showed comparatively taller height, measuring more than 300 cm, while, other ten test accessions (20.4%) were measured from 200~300 cm. There were no significant differences in the diameter of stem in the collected test accessions (2~3 cm). Of the total test accession, seven accessions showed leaf length of over 70 cm, and twenty two test accession revealed leaf length between 60-70 cm. Forty nine of the collected test accessions showed narrow and short leaves with white mid vain. These results provided additional information for the breeding programs and conservation of Sorghum bicolor as a bio-energy crop.

Persistence and Dislodgeable Residues of Chlorpyrifos and Procymidone in Lettuce Leaves under Greenhouse Condition (상추의 생산단계별 Chlorpyrifos 및 Procymidone의 잔류허용기준 설정)

  • Kim, Young-Sook;Park, Ju-Hwang;Park, Jong-Woo;Lee, Young-Deuk;Lee, Kyu-Seung;Kim, Jang-Eok
    • Korean Journal of Environmental Agriculture
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    • v.21 no.2
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    • pp.149-155
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    • 2002
  • Two pesticides commonly used for lettuce cultivation including chlorpyrifos and procymidone were subjected to a field residue trial to ensure safety of terminal residues in the harvest. After pesticides were applied at standard and double rates in a foliar spray, leaf persistence of their residues was investigated far 10 days prior to harvest. Even though far exceeded the tolerances, initial leaf residues were rapidly dissipated with time and remained only 0.4$\sim$7.2% of the residues in the harvest. As well fitted by the first-order kinetics, biological half-lives of the pesticide residues in lettuce leaves ranged 1.2$\sim$2.6 days. Slow dissipation of the residues in the harvest was observed during storage at room temperature and 4$^{\circ}C$ for 7 days. Portions of dislodgeable residues which resided in detergent washings decreased as time elapsed. Patterns in dissipation and distribution of dislodgeable residues were not largely affected by the application rate of pesticides. It is concluded that timing of pesticide application, that is, pre-harvest interval would be the first factor to determine the terminal residue level in edible portions of lettuce.

A Study of the Apply Proximity Sensor for Improved Reliability Axle Detection (열차 차축검지 신뢰성 향상을 위한 근접센서 방식 Axle Counter 적용 연구)

  • Park, Jae-Young;Choi, Jin-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.8
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    • pp.5534-5540
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    • 2015
  • This In the railway signaling system, applications of axle counter in addition to track circuit goes on increasing for detecting train position. Consequently, this paper compares sensor methods of axle counter with between geo-magnetism method and proximity sensor method. And it presents differences and results, to improve reliabilities of train detection and axle counting. Also, this article presents an applied result which is based on field experience, with regard to installation, considering attachment condition of sensor part for accurate axle counting. This study acquires expandability that is able to perform not only axle counting function but also various other functions (direction detection of train, speed detection of train, and so on). It was a result of a change of design in order to judge phase difference of sensors, to improve reliability of axle counting. Furthermore, it does not subordinate to characteristics (type, weight of train). And it is confirmed that the omission of axle counting was not occurred in 350km/h. This was the result of Lab test after the construction of transfer equipment of trial axle and Test Bed for axle counting. Both of them are self-productions. Through this, it prepares foundation which is able to apply not only to train detection but also to speed of passing trains, formation number of trains, detector locking condition - when the train passes the section of switch point, and level crossing devices. Furthermore, it would be judged to contribute safety train operation if proximity sensor method applies to the whole railway signaling system from now on.

The Degradation Patterns of Two Pesticides in Spinach by Cultivation, Storage and Washing (시금치의 생육, 저장기간 및 세척에 따른 잔류농약 분해특성)

  • Seo, Jung-Mi;Ha, Dong-Ryong;Lee, Hyang-Hee;Oh, Mu-Sul;Park, Jong-Jin;Shin, Hyeon-Wo;Kim, Eun-Sun
    • Journal of Food Hygiene and Safety
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    • v.25 no.2
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    • pp.91-99
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    • 2010
  • Two pesticides commonly used in spinach were subjected to a field residue trial to ensure safety of terminal residues in the harvest. The residual patterns of two pesticides, which were Azoxystrobin and chlorpyrifos were examined after applying with the recommended dose and their $DT_{50}$ were calculated. Also degradation patterns of pesticides at storage $4^{\circ}C$ were compared to those at $20^{\circ}C$, and removal rates of pesticides by washing spinach with water were measured. Biological half-lives of azoxystrobin and chlorpyrifos were 3.2~3.8 and 3.8~4.7 days, respectively. During the storage period, the degradation patterns were appeared more obviously at $20^{\circ}C$ than $4^{\circ}C$. Removal rates of azoxystrobin and chlorpyrifos were 9.6~90.0% and 17.7~85.8% by various washing methods.

An Analysis of Decision Making Factor by Delphi and DEMATEL Model for Decision Support Information System development -Wartime Operational Control Transition approach- (의사결정 지원 정보시스템 개발을 위한 Delphi-DEMATEL모델에 의한 의사결정 요인분석 -전작권 전환 사례를 중심으로-)

  • Park, Sangjung;Koh, Chan
    • Journal of Digital Convergence
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    • v.10 no.11
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    • pp.47-58
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    • 2012
  • This study selects political and military decision factors of Participatory Government's Wartime Operational Control(OPCON) Transition and analyzes, both quantitatively and qualitatively, the effects and relations between those factors. Previous research utilizing the Analytic Hierarchy Process(AHP) selected their decision factors based on academic data and field experience, requiring more objective analysis of the factors. For this study, we conducted a survey among security subject matter experts(SME) both online and offline. The results show that OPCON transition's decision factors were to 'recover military sovereignty', 'set the conditions for peaceful reunification' and 'improve ROK image through enhancing national power' which differs little from the previous AHP method studies. It also showed that 'recover military sovereignty' and 'set the conditions for peaceful reunification' had no relationship to each other and that the key factor that decided the OPCON Transition was actually 'recover military sovereignty' which represents the interest of the liberal party in ROK. This study finds its meaning by analyzing the decision factors of Participartory Government's OPCON Transition thorugh Delphi and DEMATEL method.

Relationship Between Heterosis and Genetic Divergence in Tongil-type Rice(Oryza sativa L.) (벼에서 RAPDs 분석에 의한 양친의 유전적 유사성과 잡종강세의 상관)

  • Ahn, Sang-Nag;Kim, Yun-Sun
    • Korean Journal of Agricultural Science
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    • v.28 no.1
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    • pp.1-7
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    • 2001
  • In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.

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A Study on Estimating Construction Cost of Apartment Housing Projects Using Genetic Algorithm-Support Vector Regression (유전 알고리즘 - 서포트 벡터 회귀를 활용한 공동주택 공사비 예측에 관한 연구)

  • Nan, Jun;Choi, Jae-Woong;Choi, Hyemi;Kim, Ju-Hyung
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.4
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    • pp.68-76
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    • 2014
  • The accurate estimation of construction cost is important to a successful development in construction projects. In previous studies, the construction cost are estimated by statistical methods. Among the statistical methods, support vector regression (SVR) has attracted a lot of attentions because of the generalization ability in the field of cost estimation. However, despite the simplicity of the parameter to be adjusted, it is not easy to find optimal parameters. Therefore, to build an effective SVR model, SVR's parameters must be set properly without additional data handling loads. So this study proposes a novel approach, known as genetic algorithm (GA), which searches SVR's optimal parameters, then adopt the parameters to the SVR model for estimating cost in the early stage of apartment housing projects. The aim of this study is to propose a GA-SVR model and examine the feasibility in cost estimation by comparing with multiple regression analysis (MRA). The experimental results demonstrate the estimating performance based on the percentage of estimations within 25% and find it can effectively do the accurate estimation without through the trial and error process.

Speed Control of Marine Gas Turbine Engine using Nonlinear PID Controller (비선형 PID 제어기를 이용한 선박용 가스터빈 엔진의 속도 제어)

  • Lee, Yun-Hyung;So, Myung-Ok
    • Journal of Navigation and Port Research
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    • v.39 no.6
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    • pp.457-463
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    • 2015
  • A gas turbine engine plays an important role as a prime mover that is used in the marine transportation field as well as the space/aviation and power plant fields. However, it has a complicated structure and there is a time delay element in the combustion process. Therefore, an elaborate mathematical model needs to be developed to control a gas turbine engine. In this study, a modeling technique for a gas generator, a PLA actuator, and a metering valve, which are major components of a gas turbine engine, is explained. In addition, sub-models are obtained at several operating points in a steady state based on the trial running data of a gas turbine engine, and a method for controlling the engine speed is proposed by designing an NPID controller for each sub-model. The proposed NPID controller uses three kinds of gains that are implemented with a nonlinear function. The parameters of the NPID controller are tuned using real-coded genetic algorithms in terms of minimizing the objective function. The validity of the proposed method is examined by applying to a gas turbine engine and by conducting a simulation.

Antibiotic Properties of Helicosporium sp. KCTC 0635BP to Rhizoctonia solani AG2-2 IV (Rhizoctonia solani AG2-2 IV에 대한 Helicosporium sp. KCTC 0635BP의 항균활성)

  • Lee, Sang Myeong;Kim, Dong Soo;Lee, Kwang-Soo;Lee, Chong-Kyu;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.202-206
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    • 2013
  • Biocontrol potential of an isolate of Helicosporium spp. against Rhizoctonia solani, Fusarium oxysporium and Phytophthora drechsleri was evaluated in vitro and in vivo. A selected biocontrol agent designated as Helicosporium 0635BP strongly inhibited growth and lysed mycelium of Rhizoctonia solani and Fusarium oxysporium on PDA. Autoclaved culture filtrate of the agent also completely inhibited growth of the turfgrass large patch pathogen, R. solani AG2-2 IV at the concentration of 50 ml $L^{-1}$. The pathogen was killed when dipped under the 20% filtrate for four hours or 50% for one hr. In a field trial, plots applied with the crude or times diluted culture filtrate showed 100% control efficacy of the turfgrass large patch as a chemical applied for a comparison. Results indicated that Helicosporium 0635BP is a promising biocontrol agent on control of the turfgrass large patch disease and its culture filtrate contained unknown heat suitable antifungal substance (s). Further studies on mass production, purification and identification of the unknown compound (s) are in progress for practical use.