• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.1041-1044
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• 1996

The plastic-film-covered hill planter is a new-type seeding machine, including tow types which are mounted by 11kw and 40 kw tractors. It is made up of fertilizing , plastic-film covering perforating film and hole seeding, soil sealing apparatus, and can work at 5-7kw/h. The plastic-film covering and seeding of cotton, corn and soybean can all use this machine. The plastic-film-covered hill planter is mainly composed of plastic film covering unit, drum-type hill-drop unit and furrow coverer, some other types are also equipped with fertilizer drill unit. It can do combined work of covering plastic film , sowing , plastic film perforating , soil covering at one time, and it is suitable to the covering plastic film as well as planting of the grandulated crops, such as cotton , corn, soybean and so on.

• Journal of Life Science
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• v.9 no.5
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• pp.628-637
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• 1999

This study was conducted to elucidate the leaf characteristics, leaf number and fertilizing level in perilla, Perilla frutescens, which was cultivated using five types organic compost with three level. Number of the harvested leaves was effective in Heulgnara with 800 kg/10 and Poongjag with 1200 kg/10 at 70 days and organic compost were the highest in Sarang followed by Heulgnara and Poongjag in order. Number of leaf harvested during the fifth times was the highest Poongjag followed by Heulgnara and Sarang in order. Leaf length and leaf width were the highest in Poongjag with 1200 kg/10 a at 70 days. Simple method for calculation of fertilizer level was N 6.09 kg and slaked lime 36.56 kg in Heulgnara 100 kg per 10 a. Recommendation level of chemical fertilizer in Heulgnara due to fertilizer method was N 275.10 kg, P 4.3 kg and K 10 kg per 10 a.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.45-51
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• 2000

According to the research of the cultivation result on the spot farmers for 5 years in order to investigate its cultivation conditions and problems of Lycium chinense Mill, the development of the variety with early mature, pest tolerance, and high yielding is urgent and it is call for the development of rooted cutting cultivation by testing of bed soil, growth regulator and cutting times. The development of cultivation method with planting year, planting density, supplementary period and amounts of supplementary of Lycium chinense Mill planted in growing field are required and for the disease and the effective control of disease and insect pest resistance is needed by auxotrophic fertilizing and organic agricultural method, met by agricultural chemicals.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2000.10a
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• pp.605-610
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• 2000

The effect of slow release fertilizer application on ponded and percolation water in paddies were evaluated at the experimental plots during irrigation period. We laid out three experimental plots such as standard, 100% slow-release and 80% slow-release. For ponded water, the concentrations of T-N in slow-release plots slowly increase after fertilizing and decrease slowly. The concentration of COD in percolated water was independent of the type of plots. Amount of nitrogen uptake of rice plant in a standard plot was highest at the middle or end of July and was higher than the other plots.

• Journal of Aquaculture
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• v.18 no.2
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• pp.81-85
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• 2005

We isolated two parvalbumin cDNAS by expressed sequence tag analysis (1,577 ESTs in total) from the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae). Two isoforms of parvalbumin genes showed high similarity to those of carp at 88% and 91% amino acid residues identity, respectively, and showed 79.8% similarity between two parvalbumin isoforms. Of 1,577 ESTs from R. marmroatus sequenced, parvalbumin 1 gene was most abundant. This gene was strongly expressed in the order of muscle, eye, and brain, while it was expressed slightly in other tissues. In this paper, we discussed on the R. marmoratus parvalbumin genes on its sequence and basic characteristics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.84-88
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• 1990

This experiment was conducted to evaluate the effect of cutting frequency and nitrogen fertilization on $(NO_3$-N leaching losses under 12 years mixture permanent meadow, The results of this study were summarized as follows: 1. There was no difference in the $(NO_3$-N content of soil water within 1 m soil depth during the whole experimental period. It means that the content of $(NO_3$-N leaching losses was not influenced by botanical composition, cutting frequency and nitrogen fertilization in this experiment. 2. The level of $(NO_3$-N content during the whole experimental period was not in excess of 0.5 ppm level.

• Korean Journal of Environmental Agriculture
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• v.22 no.1
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• pp.41-46
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• 2003

The suitability of the life Cycle Assessment (LCA) methodology to analyze the environmental impact of rice cultivation with different fertilizing systems is investigated. The arst part of an LCA is an inventory of parameters used and emissions released due to the system under investigation. In the following step, the Life Cycle Impact Assessment the inventory data were analyzed and aggregated in order to finally get one index representing the total environmental burden. For the life Cycle Impact Assessment (LCIA) the Eco-indicator 95 method has been chosen because this is well documented and regularly applied impact assessment method. The resulting index is called Eco-indicator value. The higher the Eco-indicator value the stronger is the total environmental impact of an analyzed fertilizing system. The rice field experiment conducted in middle parts of korea was chosen as an example for the life cycle impact analysis. In this experiment the treatments were consisted of none fertilizer plot (NF), standard fertilizer plot (SF) applied chemical fertilizers based on soil chemical analysis before rice transplanting, and efflux fertilized plot (EF) applied with pig wastes fermented as the same rates of SF plot as basis on total nitrogen content. The obtained Eco-indicator values were clearly different among the treatments in the rice trial. The total Eco-indicator values for SF and EF have been observed 58 and 38% relative to the NF, respectively. For all the treatments the environmental effects of eutrophication contributed most to the total Eco-indicator value. The results appeared that the LCA methodology is basically suitable to assess the environmental impact associated with different fertilizer applications for rice cultivation. A comparative analysis of the fertilizing system's contribution to global warming and eutrophication is possible.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.4
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• pp.286-293
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• 1993

This study was carried out to determine the effects of the fertilization levels and oversowing treatment on liveweight gain of glazing cattle, changs of botanical composition, and dry matter(DM) yield in tall fescue dominant mixed pasture during the grazing period. The treatments were T$_1$(low fertilizing; 120-100-100 kg/ha), T$_2$(medium fertilizing; 280-200-200 kg/ha) and T$_3$(medium fertilizing+oversowing). The botanical composition of tail festuce was increased in T$_1$ and that of tall fescue, orchardgrass and pernnial ryegrass in T$_3$ was 30.5%, 23.8% and 24.1%, respectively. The total forage DM yield was the highest in T$_3$, and the average stocking rate (animal unit; AU) per day during the grazing period in T$_1$, T$_2$ and T$_3$ was 2.4 AU. 3.0 Au and 3.3 AU, respectively. The total grazing days (animal unit day; AUD) in T$_3$(664 AUD) was higher than that of T$_1$, and T$_2$. There is no significant difference in average daily liveweight gain per head among the treatments but daily liveweight gain per ha in T$_3$ was higher than that of T$_1$, and T$_2$. The total liveweight gain per ha during the grazing period in T$_1$, T$_2$ and T$_3$ was 601kg. 762kg and 877kg, respectively. The herbage consumption per day per 100kg LW was similer among the treatments but crude protein, P, K and Ca contents in herbage were increased with medium fertilization levels(T$_2$) and with oversowing(T$_3$).

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.409-419
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• 2001

This study was to examine whether the in vitro friability, motility and intact acrosome of frozen-thawed bovine and human sperm can be improved by adding Pentoxifylline (PF) or Fertilization Promoting Peptide (FPP). Human semen was frozen ultra-rapidly using Test yolk-buffer (TYB) freezing medium. Additive (PF, FPP) effects in frozen-thawed bovine and human sperm were analyzed by microscopic count for sperm motility and coomassie brilliant blue staining method f3r sperm acrosome intact. The in vitro motility of frozen-thawed bovine sperm with 5 mM PF treatment group (50.0%) was significantly higher than that of control (34.0%) (P＜0.05). In the frozen-thawed bovine sperm was examined, the intact acrosome rate of 50 nM FPP treatment (49.0%) was significantly higher than those of control (30.0%) and 25 nM FPP (38.0%) treatment groups (P＜0.01). In human semen, when in vitro motility of sperm with PF addition prior to freezing was examined, the result of 5 mM treatment group (51.0%) was significantly higher than those of control and 2.5 mM treatment group (39.0, 40.0%) (P＜0.01). In addition, 50 nM (75.5%) FPP adding in all treatment procedures for human semen freezing (before freezing, freezing and after thawing) was significant effect on maintenance of the sperm intact acrosome percentage (control: 45.0; 25 nM: 53.0; 100 nM: 68.0%) (P＜0.01). Also, the intact acrosome rate of human sperm with FPP (65.0%) was significantly higher than that with PF (43.0%) (P＜0.05), although sperm motility was slightly higher in PF treatment group. These results suggest that improved sperm motility and intact acrosome of frozen thawed bovine and human sperm can be obtained by addition of PF or FPP, and that the enhanced in vitro viability, motility and intact acrosome can be obtained by addition of FPP in all semen freezing procedures.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.327-337
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• 2001

This study investigated the effects of superoxide dismutase (SOD) on lipid peroxidation and fertilizing ability in vitro of boar spermatozoa frozen-thawed. The percentages of motile sperm were highest when SOD of 10 units/$m\ell$ was added to washing medium for spermatozoa. However, the rates of motile sperm were not significantly different in different concentrations of SOD. On the other hand, the motile rates of sperm washed with SOD were lower in sperm inculbated for 120 min than 30 min regardless of the different concentrations of SOD. The percentage of spermatozoa that reached acrosome reaction were increased with incubation periods prolonged. No significant differences, however, were observed in acrosome reaction rates between sperm incubated with and without SOD supplementation for 0, 60 and 120 min. When oocyies inseminated with different concentrations of SOD, the penetration rates were significantly (P＜0.05) higher in medium with 1 unit/$m\ell$ than 0, 10 and 100 units/$m\ell$ of SOD. However, the proportions of polyspermit oocytes were significantly (P＜0.05) lower in medium with 10 and 100 units/$m\ell$ than 0 unit/$m\ell$ of SOD. In another experiment, the sperm suspension were also treated with different concentrations of SOD and were assayed far sulfhydryl(-SH) group content. In the groups treated with 100 units/$m\ell$ of SOD, sperm-SH group were higher than another groups. However, sperm-SH group content were not siginificantly different in spermatozoa treated with different concentrations of SOD. Under the same conditions, the lipid peroxidation of sperm was evaluated on the basis of malondialdehyde production. The addition of SOD to sperm suspension decreased the formation or malondialdehyde. However, there were not significantly different in sperm treated with different concentrations of SOD. The activity of sperm binding to zona pellucida was also evaluated through binding to salt-stored porcine oocytes. The sperm binding to zona pellucida were gradually increased with SOD concentrations added. The number of spermatozoa binded to zona pellucida were significantly (P＜0.05) higher in medium with 100 units/$m\ell$ than 0 units/$m\ell$ of SOD. These findings suggested that SOD cause an enhancement penetrarion ability and sperm zona binding in boar spermatozoa frozen-thawed.